LOCUS pAMG216 4700 bp DNA circular 13-FEB-2019 DEFINITION . ACCESSION urn.local...29k-9k3unee KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers rep_origin 6..1540 /label="putative minimal C. thermocellum ori" CDS complement(81..1085) /label="repB" misc_feature 530..533 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 534..537 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 779..782 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 982..985 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" repeat_region 1174..1539 /label="pNW33N Repeat 1" CDS complement(1214..1462) /label="putative mob/pre fragment" misc_feature 1328..1331 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" primer 1415..1433 /label="NJ150" misc_feature 1430..1433 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 1481..1484 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 1502..1505 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 1571..2218 /label="P-gapD" /note="Geneious type: promoter prokaryotic" misc_feature 1583..1586 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 1602..1605 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 1655..1658 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2154..2157 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" CDS 2219..2869 /label="cat" misc_feature 2652..2655 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2804..2807 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2886..2889 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" CDS 2887..3432 /label="hpt (putative)" terminator 3541..3794 /label="CYC1 term" misc_feature 3543..3546 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 3725..3728 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 3977..3980 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" rep_origin 3978..4651 /label="pUC origin" misc_feature 4697..4700 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" ORIGIN 1 ctagtgaatt taggaggctt acttgtctgc tttcttcatt agaatcaatc cttttttaaa 61 agtcaatccc gtttgttgaa ctactcttta ataaaataat ttttccgttc ccaattccac 121 attgcaataa tagaaaatcc atcttcatcg gctttttcgt catcatctgt atgaatcaaa 181 tcgccttctt ctgtgtcatc aaggtttaat tttttatgta tttcttttaa caaaccacca 241 taggagatta accttttacg gtgtaaacct tcctccaaat cagacaaacg tttcaaattc 301 ttttcttcat catcggtcat aaaatccgta tcctttacag gatattttgc agtttcgtca 361 attgccgatt gtatatccga tttatattta tttttcggtc gaatcatttg aacttttaca 421 tttggatcat agtctaattt cattgccttt ttccaaaatt gaatccattg tttttgattc 481 acgtagtttt ctgtattctt aaaataagtt ggttccacac ataccaatac atgcatgtgc 541 tgattataag aattatcttt attatttatt gtcacttccg ttgcacgcat aaaaccaaca 601 agatttttat taattttttt atattgcatc attcggcgaa atccttgagc catatctgac 661 aaactcttat ttaattcttc gccatcataa acatttttaa ctgttaatgt gagaaacaac 721 caacgaactg ttggcttttg tttaataact tcagcaacaa ccttttgtga ctgaatgcca 781 tgtttcattg ctctcctcca gttgcacatt ggacaaagcc tggatttaca aaaccacact 841 cgatacaact ttctttcgcc tgtttcacga ttttgtttat actctaatat ttcagcacaa 901 tcttttactc tttcagcctt tttaaattca agaatatgca gaagttcaaa gtaatcaaca 961 ttagcgattt tcttttctct ccatggtctc acttttccac tttttgtctt gtccactaaa 1021 acccttgatt tttcatctga ataaatgcta ctattaggac acataatatt aaaagaaacc 1081 cccatctatt tagttatttg tttggtcact tataacttta acagatgggg tttttctgtg 1141 caaccaattt taagggtttt caatacttta aaacacatac ataccaacac ttcaacgcac 1201 ctttcagcaa ctaaaataaa aatgacgtta tttctatatg tatcaagaat agaaagaact 1261 cgtttttcgc tacgctcaaa acgcaaaaaa agcactcatt cgagtgcttt ttcttatcgc 1321 tccaaatcat gcgatttttt cctctttgct tttctttgct cacgaagttc tcgatcacgc 1381 tgcaaaacat cttgaagcga aaaagtattc ttcttttctt ccgatcgctc atgctgacgc 1441 acgaaaagcc ctctaggcgc ataggaacaa ctcctaaatg catgtgaggg gttttctcgt 1501 ccatgtgaac agtcgcatac gcaatatttt gtttcccata gctagtaagc ttggatcctc 1561 gcgaggccgg ccagtattct gacatgggtg tatcaataac ccatgcgttt ccgtattgta 1621 tcggaatggt ttcggacagg gcggtgggaa tagacatgga aaagattttt ttgcccgagg 1681 atgcattgat aaagtatttc ttttccgaaa gagaggaaaa gattctaaag agttttggaa 1741 atactgatga atattgtgtg cagagtacaa ttctatggac aagaaaagag gctttgtcaa 1801 aactttttcg tctgggaatg aggatggatt ttaaaaagct ggatactttg gaggacgagg 1861 tggtttttca ggaaacaaac agggcgcgtc tgttttcttt tatatgcaat aattactgta 1921 tctctctggc attgccaggt tttaataaag attaaaatta ttgactagaa ataaaaaaat 1981 tgtccataat attaatggac aaaaaaacaa agaattacat caaaggaaga taaaaatact 2041 ttgttaaaaa attaattatt ttttatctaa actattgaaa atgaaaataa aataatataa 2101 aatgaatcat agtgcaagag atacttgcca gaggatgaat attttactgc attcatgctt 2161 tatggcagct aatagaggca ttaaattaaa ttttaattta caataggagg cgatattaat 2221 gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt 2281 gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa 2341 cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat 2401 aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag gttattggga 2461 taagttagag ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg 2521 gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa 2581 atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct 2641 ttctattatt ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa 2701 ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt 2761 accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat 2821 gaactctatt caggaattgt cagataggcc taatgactgg cttttataat aaaggaggtc 2881 gacgtcatgg aaaatttatc aaaagacatc gatgaaattt tgatcacaga agaagaactt 2941 aaggaaaaga taaaagagct tgggaggcaa atcacaaaag actacaaagg gaaaaatttg 3001 atgttggtag gagttttaaa aggtgcttta atgtttatgg ctgatttgtc aagacacata 3061 gatttgcctt tatcacttga ttttatggct gtttccagct atggaagctc aactcattca 3121 tcaggaatag taaagataat caaagatctt gatataagca tagaaggcaa agatgttctg 3181 attgtggaag acataattga cagcggtttg actttgtctt acttaaggga aactttactt 3241 ggaaggaagc caaaaagcct gaaaatatgc acaatattag acaaaccgga gagaagagaa 3301 gcatctgtaa aagtcgatta tgtaggattt aagatacctg ataagtttgt cgtgggttat 3361 ggattggact ttgatgaaaa gtacaggaac cttcctttta taggcgtttt gaaacctgaa 3421 atgtacagct aagcccgggc ctcgagaaaa caaaaggctc agtcggaaga ctgggccttt 3481 tgttttggta ccgaattcgg cgcgcctcag cgtttaaacc cgctgatcct agagggccgc 3541 atcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc 3601 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg 3661 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg 3721 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct 3781 ttaatttgca agctgcggcc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg 4021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4441 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4681 gttaagggat tttggtcatg //