LOCUS pNJ020 3561 bp DNA circular 13-FEB-2019 DEFINITION . ACCESSION urn.local...aqo-a1od340 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers rep_origin complement(66..910) /label="p15A ori" misc_feature 125..128 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 640..643 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 644..1098 /created_by="lrf" /modified_by="lrf" /label="Methylated" misc_feature 644..705 /created_by="lrf" /modified_by="lrf" /label misc_feature 702..705 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 706..1098 /created_by="lrf" /modified_by="lrf" /label="Unmethylated" misc_recomb 937..984 /modified_by="lrf" /label misc_feature 985..1159 /label="PgapDH" /note="Geneious type: promoter prokaryotic" misc_feature 1095..1098 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" CDS 1160..1804 /label="cat2" misc_recomb 1811..1856 /label="FRT" rep_origin 1951..3485 /label="putative minimal C. thermocellum ori" CDS complement(2026..3030) /label="repB" misc_feature 2475..2478 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2479..2482 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2724..2727 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 2927..2930 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" repeat_region 3119..3484 /label="pNW33N Repeat 1" CDS complement(3159..3407) /label="putative mob/pre fragment" misc_feature 3273..3276 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 3375..3378 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 3426..3429 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" misc_feature 3447..3450 /Recognition_pattern="CATG^" /annotation_group="CATG" /label="NlaIII" /note="Geneious type: restriction site" ORIGIN 1 ctagttgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 61 ggcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt 121 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg 181 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg 241 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg 301 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc 361 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg 421 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg 481 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag 541 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc 601 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca 661 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta 721 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga 781 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga 841 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa 901 atatttctag gcaccaataa ctgccttaaa gatatcgaag ttcctatact ttctagagaa 961 taggaacttc ggaataggaa cttcttaaaa aattaattat tttttatcta aactattgaa 1021 aatgaaaata aaataatata aaatgaatca tagtgcaaga gatacttgcc agaggatgaa 1081 tattttactg cattcatgct ttatggcagc taatagaggc attaaattaa attttaattt 1141 acaataggag gtgatattaa tgaacttcaa taagattgat ttagacaact ggaagagaaa 1201 agagatattc aatcattatt tgaaccaaca aacgactttt agtataacca cagagattga 1261 tattagtgtt ttatatagga acataaaaca agaaggatat aagttttacc ctgcctttat 1321 tttcttagtg acaagggtga taaactcaaa tacagcgttt agaactggtt acaatagcga 1381 cggagagtta ggttattggg ataagttaga gccactttat accatttttg atggtgtatc 1441 taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt tttatgattt 1501 atacctttct gatgtagaga aatataatgg ttcggggaaa ctttttccca aaacacctat 1561 acctgaaaat gctttttctc tttctattat tccttggact tcatttactg ggttcaacct 1621 gaatatcaat aataatagta actaccttct acccattatt acagcaggaa agttcatcaa 1681 taaaggtaat agtatatatt taccgctatc tttacaggtc catcattctg tttgtgatgg 1741 ttatcacgca ggattgttta tgaactctat tcaggaactt tcagatagac ctaatgactg 1801 gcttttataa gaagttccta tactttctag agaataggaa cttcggaata ggaactaagg 1861 aggatattca tatgcccggg gcggccgcgg taccaagctt caattttatt aaagttcatt 1921 tgatatgcct cctaaatttt tatctaaagt gaatttagga ggcttacttg tctgctttct 1981 tcattagaat caatcctttt ttaaaagtca atcccgtttg ttgaactact ctttaataaa 2041 ataatttttc cgttcccaat tccacattgc aataatagaa aatccatctt catcggcttt 2101 ttcgtcatca tctgtatgaa tcaaatcgcc ttcttctgtg tcatcaaggt ttaatttttt 2161 atgtatttct tttaacaaac caccatagga gattaacctt ttacggtgta aaccttcctc 2221 caaatcagac aaacgtttca aattcttttc ttcatcatcg gtcataaaat ccgtatcctt 2281 tacaggatat tttgcagttt cgtcaattgc cgattgtata tccgatttat atttattttt 2341 cggtcgaatc atttgaactt ttacatttgg atcatagtct aatttcattg cctttttcca 2401 aaattgaatc cattgttttt gattcacgta gttttctgta ttcttaaaat aagttggttc 2461 cacacatacc aatacatgca tgtgctgatt ataagaatta tctttattat ttattgtcac 2521 ttccgttgca cgcataaaac caacaagatt tttattaatt tttttatatt gcatcattcg 2581 gcgaaatcct tgagccatat ctgacaaact cttatttaat tcttcgccat cataaacatt 2641 tttaactgtt aatgtgagaa acaaccaacg aactgttggc ttttgtttaa taacttcagc 2701 aacaaccttt tgtgactgaa tgccatgttt cattgctctc ctccagttgc acattggaca 2761 aagcctggat ttacaaaacc acactcgata caactttctt tcgcctgttt cacgattttg 2821 tttatactct aatatttcag cacaatcttt tactctttca gcctttttaa attcaagaat 2881 atgcagaagt tcaaagtaat caacattagc gattttcttt tctctccatg gtctcacttt 2941 tccacttttt gtcttgtcca ctaaaaccct tgatttttca tctgaataaa tgctactatt 3001 aggacacata atattaaaag aaacccccat ctatttagtt atttgtttgg tcacttataa 3061 ctttaacaga tggggttttt ctgtgcaacc aattttaagg gttttcaata ctttaaaaca 3121 catacatacc aacacttcaa cgcacctttc agcaactaaa ataaaaatga cgttatttct 3181 atatgtatca agaatagaaa gaactcgttt ttcgctacgc tcaaaacgca aaaaaagcac 3241 tcattcgagt gctttttctt atcgctccaa atcatgcgat tttttcctct ttgcttttct 3301 ttgctcacga agttctcgat cacgctgcaa aacatcttga agcgaaaaag tattcttctt 3361 ttcttccgat cgctcatgct gacgcacgaa aagccctcta ggcgcatagg aacaactcct 3421 aaatgcatgt gaggggtttt ctcgtccatg tgaacagtcg catacgcaat attttgtttc 3481 ccatactgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 3541 cttccgcttc ctcgctcact a //