This PDF file includes:

• Fig. S1. Characterization of the methacrylated hyaluronic acid (MeHA) macromers.
• Fig. S2. The mechanical characterizations of the MeHA hydrogels conjugated with various groups of peptide.
• Fig. S3. Foxy5 peptide-conjugated hydrogels upregulate the expression of mechanotransduction signaling molecules in the seeded hMSCs.
• Fig. S4. The promoting effect of conjugated Foxy5 peptide on the osteogenesis of hMSCs is dependent on ROCK and non-muscle myosin II activities.
• Fig. S5. Conjugated Foxy5 peptides enhance the expression of canonical Wnt-related genes of hMSCs seeded in the 3D hydrogel.
• Fig. S6. The hydrogel-conjugated Foxy5 peptide promotes the osteogenesis in a wide range of hydrogel substrate stiffness.
• Fig. S7. The conjugated Foxy5 peptide promotes the osteogenesis of the hMSCs from multiple donors.
• Fig. S8. The conjugated Foxy5 peptide promotes the mechanotransduction and osteogenesis of the seeded rMSCs.
• Table S1. The sequence of the primers and probes used for real-time PCR are listed.
• Table S2. The donor information of the hMSCs used for the in vitro experiments is listed.

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