This PDF file includes:

• Fig. S1. Purified sample of P68 contains native virions, particles in process of genome release, and empty particles; attachment of P68 virions to S. aureus cell walls; and interactions of P68 virions with liposomes.
• Fig. S2. Resolution and interpretability of cryo-EM reconstructions.
• Fig. S3. Details of P68 head.
• Fig. S4. Incorporation of P68 portal complex into capsid and changes in the structure of P68 portal complex upon genome release.
• Fig. S5. Structures of P68 tail fiber and tail spike.
• Fig. S6. Schemes of cryo-EM reconstruction strategies.
• Table S1. Cryo-EM structure quality indicators.
• Table S2. List of P68 proteins.
• Table S3. HHpred searches for homologs of P68 tail fiber, head fiber, tail knob, and tail spike.
• Table S4. X-ray structure quality indicators.

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