The PDF file includes:
- Fig. S1. Sample preparation and cryo-EM images of P-Rex1–Gβγ.
- Fig. S2. Cryo-EM data processing overview.
- Fig. S3. Validation of cryo-EM reconstruction.
- Fig. S4. Cryo-EM map of the P-Rex1–Gβγ complex from fig. S2E showing the P-Rex1 N-terminal region and loops extending from the IP4P domain core.
- Fig. S5. Comparison of the independently crystallized P-Rex1 PDZ1 domain (PDB: 3QIK) with the PDZ1 domain in the context of the P-Rex1 C-terminal core.
- Fig. S6. Full-length P-Rex1 lacks phosphatase activity.
- Fig. S7. Detailed view of P-Rex1–Gβγ interaction sites.
- Fig. S8. Effects of PIP3 and Gβγ on guanine nucleotide exchange accelerated by P-Rex1.
- Fig. S9. HDX-MS of P-Rex1 alone.
- Table S1. Cryo-EM data collection, refinement, and validation statistics.
- Legends for data files S1 and S2
Download PDF
Other Supplementary Material for this manuscript includes the following:
- Data file S1 (.pdf format). Ribbon maps representing HDX-MS experiments.
- Data file S2 (Microsoft Excel format). Kinetic data for HDX-MS experiments.