The PDF file includes:

• Fig. S1. Venus-Na_V1.7-BAD and Halo-Na_V1.7 display WT currents and express in cultured DRG neurons (related to Figs. 1A and 2A).
• Fig. S2. Venus-Na_V1.7-BAD surface labeling does not display bleed-through, and DRG neurons display autofluorescence (related to Fig. 1).
• Fig. S3. Venus-Na_V1.7-BAD and Halo-Na_V1.7 nanoclusters are visible in DRG axons (related to Fig. 1).
• Fig. S4. Methods for estimating number of channels per nanocluster (related to Fig. 1G).
• Fig. S5. Retrograde, but not anterograde, vesicles can be visualized using the fluorescent protein signal (related to Fig. 2).
• Fig. S6. Rab6 expression in cultured DRG neurons (related to Fig. 3).
• Fig. S7. Fluorescence intensity for individual JF549-Halo molecules (related to Fig. 5).
• Fig. S8. Modification of MFCs (related to Materials and Methods).
• Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi format). Anterograde trafficking of Halo-Na_V1.7.
• Movie S2 (.avi format). Anterograde trafficking of Halo-Na_V1.7 under control conditions and after exposure to IM.