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["Table S3"]
["A) Results of single gene knock out experiments. Listed are genes that caused a suboptimal growth rate when knocked out in the first part and in the second part genes without phenotype in the model, but mentioned in the genetic screens of zebrafish. Literature references are: 1= Amsterdam et al., 2004, 2= Golling et al., 2002, \n3=Amsterdam et al., 1999, 4=Driever et al., 1996, 5=Brockerhoff et al., 1995, 6=Muto et al., 2005, 7=Covassin et \nal., 2009, 8=Iwanami et al., 2016, *=found to have a knock-down phenotype mentioned in the ZFIN database."]
[null,"Single knockouts"]
[null,"No/reduced growth"]
[null,"Gene name","Flux phenotype","Growth rate fraction","Mentioned in"," Involved subsystems  ","Reactions statuses ZebraGEM 2.0","Diseases associated with human homologs"]
[null,"paics","lethal",0,1,"Purine synthesis","present"]
[null,"tyms","lethal",0,1,"Nucleotide interconversion","present"]
[null,"pah","lethal",0,6,"Tetrahydrobiopterin metabolism","present","Phenylketonuria; [Hyperphenylalaninemia, non-PKU mild]"]
[null,"uqcrb","lethal",0,"*","Oxidative phosphorylation","added","Mitochondrial complex III deficiency, nuclear type 3"]
[null,"hsd17b4","lethal",0,"*","Phenylalanine metabolism, Tryptophan metabolism, Bile acid synthesis, Fatty acid synthesis, Fatty acid oxidation, Valine, leucine, and isoleucine metabolism, Cholesterol metabolism","added, present","D-bifunctional protein deficiency; Perrault syndrome 1"]
[null,"dhfr","lethal",0,"*","Folate metabolism","present","Megaloblastic anemia due to dihydrofolate reductase deficiency"]
[null,"fh","lethal",0,"*","Citric acid cycle","present","Fumarase deficiency; Leiomyomatosis and renal cell cancer"]
[null,"gmps","lethal",0,"*","Bile acid synthesis, Miscellaneous, Nucleotide interconversion","present"]
[null,"sptlc3","lethal",0,"*","Sphingolipid metabolism","present"]
[null,"cad","lethal",0,"1, *","Pyrimidine synthesis, Glutamate metabolism","present","Epileptic encephalopathy, early infantile, 50"]
[null,"cdipt","lethal",0,"1, *","Glycerophospholipid metabolism","present"]
[null,"rrm1","lethal",0,"1,2,3","Glutathione metabolism, Nucleotide interconversion","present"]
[null,"abat","lethal",0,null,"beta-Alanine metabolism, Pyrimidine catabolism, Glutamate metabolism","present","GABA-transaminase deficiency"]
[null,"acacb","lethal",0,null,"Fatty acid oxidation, Biotin metabolism","added, present"]
[null,"adsl","lethal",0,null,"Purine synthesis, Nucleotide interconversion","present","Adenylosuccinase deficiency"]
[null,"aldh6a1","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Propanoate metabolism","present","Methylmalonate semialdehyde dehydrogenase deficiency"]
[null,"asns","lethal",0,null,"Alanine and aspartate metabolism, Glutamate metabolism","present","Asparagine synthetase deficiency"]
[null,"atic","lethal",0,null,"Purine synthesis","present","AICA-ribosiduria due to ATIC deficiency"]
[null,"cmpk","lethal",0,null,"Nucleotide interconversion","present"]
[null,"cox4i1","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"cox6a1","lethal",0,null,"Oxidative phosphorylation","added","Charcot-Marie-Tooth disease, recessive intermediate D"]
[null,"cox6a2","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"cox6b1","lethal",0,null,"Oxidative phosphorylation","added","Mitochondrial complex IV deficiency"]
[null,"cox6c","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"cox7b","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"cox7c","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"cox8a","lethal",0,null,"Oxidative phosphorylation","added","?Mitochondrial complex IV deficiency"]
[null,"crls1","lethal",0,null,"Glycerophospholipid metabolism","present"]
[null,"cth","lethal",0,null,"Methionine and cysteine metabolism, Selenoamino acid metabolism","present","Cystathioninuria; Homocysteine, total plasma, elevated "]
[null,"ctps1a","lethal",0,null,"Pyrimidine synthesis, Nucleotide interconversion","present","Immunodeficiency 24"]
[null,"cyc1","lethal",0,null,"Oxidative phosphorylation","added","Mitochondrial complex III deficiency, nuclear type 6"]
[null,"cyp51","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present"]
[null,"CYTB","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"dhcr24","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present","Desmosterolosis"]
[null,"dhcr7","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present","Smith-Lemli-Opitz syndrome"]
[null,"dhodh","lethal",0,null,"Pyrimidine synthesis","present","Miller syndrome"]
[null,"dtymk","lethal",0,null,"Nucleotide interconversion","present"]
[null,"ebp","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present","Chondrodysplasia punctata, X-linked dominant; MEND syndrome"]
[null,"fasn","lethal",0,null,"Fatty acid synthesis, Fatty acid oxidation","added, present"]
[null,"fdft1","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present","Squalene synthase deficiency"]
[null,"gart","lethal",0,null,"Purine synthesis","present"]
[null,"idi1","lethal",0,null,"Cholesterol metabolism, Squalene and cholesterol synthesis","added, present"]
[null,"kdsr","lethal",0,null,"Sphingolipid metabolism","present","Erythrokeratodermia variabilis et progressiva 4"]
[null,"lss","lethal",0,null,"Cholesterol metabolism","present","Cataract 44; Hypotrichosis 14"]
[null,"mcee","lethal",0,null,"Valine, leucine, and isoleucine metabolism","present","Methylmalonyl-CoA epimerase deficiency"]
[null,"mut","lethal",0,null,"Valine, leucine, and isoleucine metabolism","present","Methylmalonic aciduria, mut(0) type"]
[null,"mvda","lethal",0,null,"Cholesterol metabolism","present","Porokeratosis 7, multiple types"]
[null,"mvk","lethal",0,null,"Squalene and cholesterol synthesis","present","Hyper-IgD syndrome; Mevalonic aciduria; Porokeratosis 3, multiple types"]
[null,"nsdhl","lethal",0,null,"Cholesterol metabolism","added, present","CHILD syndrome; CK syndrome"]
[null,"pfas","lethal",0,null,"Purine synthesis","present"]
[null,"pisd","lethal",0,null,"Glycerophospholipid metabolism","present"]
[null,"pmvk","lethal",0,null,"Squalene and cholesterol synthesis","present","Porokeratosis 1, multiple types"]
[null,"ppat","lethal",0,null,"Purine synthesis","present"]
[null,"rpia","lethal",0,null,"Pentose phosphate pathway","present","?Ribose 5-phosphate isomerase deficiency"]
[null,"sc5d","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present","Lathosterolosis"]
[null,"sdha","lethal",0,null,"Citric acid cycle","present","Cardiomyopathy, dilated, 1GG; Leigh syndrome; Mitochondrial respiratory chain complex II deficiency; Paragangliomas 5"]
[null,"sdhb","lethal",0,null,"Citric acid cycle","present","Gastrointestinal stromal tumor; Paraganglioma and gastric stromal sarcoma; Paragangliomas 4; Pheochromocytoma"]
[null,"sdhc","lethal",0,null,"Citric acid cycle","present","Gastrointestinal stromal tumor; Paraganglioma and gastric stromal sarcoma; Paragangliomas 3"]
[null,"sgms2","lethal",0,null,"Sphingolipid metabolism","present"]
[null,"si:ch1073-325m22.2","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"slc25a20","lethal",0,null,"Transport, mitochondrial, Fatty acid oxidation","added, present","Carnitine-acylcarnitine translocase deficiency"]
[null,"slc27a4","lethal",0,null,"Transport, extracellular","added","Ichthyosis prematurity syndrome"]
[null,"soat1","lethal",0,null,"Cholesterol metabolism","present"]
[null,"sptlc1","lethal",0,null,"Sphingolipid metabolism","present","Neuropathy, hereditary sensory and autonomic, type IA"]
[null,"sqlea","lethal",0,null,"Cholesterol metabolism","present"]
[null,"tm7sf2","lethal",0,null,"Steroid metabolism, Cholesterol metabolism","present"]
[null,"txnrd1","lethal",0,null,"Selenoamino acid metabolism, Nucleotide interconversion","present"]
[null,"umps","lethal",0,null,"Pyrimidine synthesis, Miscellaneous","present","Orotic aciduria"]
[null,"uqcr10","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"uqcrc1","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"uqcrc2b","lethal",0,null,"Oxidative phosphorylation","added","Mitochondrial complex III deficiency, nuclear type 5"]
[null,"uqcrfs1","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"uqcrh","lethal",0,null,"Oxidative phosphorylation","added"]
[null,"uqcrq","lethal",0,null,"Oxidative phosphorylation","added","Mitochondrial complex III deficiency, nuclear type 4"]
[null,"hmgcs1","reduced",0.404,null,"Cholesterol metabolism","present"]
[null,"atp5o","reduced",0.509,1,"Oxidative phosphorylation","added"]
[null,"atp5a1","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5c1","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5d","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5e","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5f1","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5h","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5l","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"atp5s","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"ATP6","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"ATP8","reduced",0.509,null,"Oxidative phosphorylation","added"]
[null,"pgk1","reduced",0.647,"*","Glycolysis/gluconeogenesis","present","Phosphoglycerate kinase 1 deficiency"]
[null,"aclya","reduced",0.813,"*","Citric acid cycle","present"]
[null,"slc25a1b","reduced",0.813,null,"Transport, mitochondrial","present","Combined D-2- and L-2-hydroxyglutaric aciduria; ?Myasthenic syndrome, congenital, 23, presynaptic"]
[null,"gde1","reduced",0.827,null,"Glycerophospholipid metabolism","added"]
[null,"cs","reduced",0.831,null,"Citric acid cycle","present"]
[null,"cept1b","reduced",0.872,null,"Glycerophospholipid metabolism","present"]
[null,"dbt","reduced",0.89,"*","Valine, leucine, and isoleucine metabolism, Propanoate metabolism","added, present","Maple syrup urine disease, type II"]
[null,"dldh","reduced",0.89,"*","Propanoate metabolism, Glycine, serine, alanine and threonine metabolism, Glycolysis/gluconeogenesis, Lysine metabolism, Citric acid cycle, Valine, leucine, and isoleucine metabolism","added, present","Dihydrolipoamide dehydrogenase deficiency"]
[null,"bckdha","reduced",0.89,null,"Valine, leucine, and isoleucine metabolism, Propanoate metabolism","added, present","Maple syrup urine disease, type Ia"]
[null,"bckdhb","reduced",0.89,null,"Valine, leucine, and isoleucine metabolism, Propanoate metabolism","added, present","Maple syrup urine disease, type Ib"]
[null,"hibch","reduced",0.89,null,"Valine, leucine, and isoleucine metabolism, beta-Alanine metabolism","present","3-hydroxyisobutryl-CoA hydrolase deficiency"]
[null,"cps1","reduced",0.91,"*","Glutamate metabolism","present","Carbamoylphosphate synthetase I deficiency; {Pulmonary hypertension, neonatal, susceptibility to}; {Venoocclusive disease after bone marrow transplantation} "]
[null,"asl","reduced",0.91,null,"Alanine and aspartate metabolism","present","Argininosuccinic aciduria"]
[null,"ass1","reduced",0.91,null,"Alanine and aspartate metabolism","present","Citrullinemia"]
[null,"otc","reduced",0.91,null,"Urea cycle","present","Ornithine transcarbamylase deficiency"]
[null,"slc25a15a","reduced",0.91,null,"Transport, mitochondrial","present","Hyperornithinemia-hyperammonemia-homocitrullinemia syndrome"]
[null,"bcat1","reduced",0.934,null,"Valine, leucine, and isoleucine metabolism","present","?Hyperleucinemia-isoleucinemia or hypervalinemia"]
[null,"cds1","reduced",0.947,"*","Glycerophospholipid metabolism","present"]
[null,"si:ch1073-100f3.2","reduced",0.961,null,"Transport, mitochondrial","present"]
[null,"slc5a1","reduced",0.967,null,"Transport, extracellular","present","Glucose/galactose malabsorption"]
[null,"arg1","reduced",0.968,null,"Urea cycle","present","Argininemia"]
[null,"hal","reduced",0.97,"*","Histidine metabolism","present","[Histidinemia]"]
[null,"ftcd","reduced",0.97,null,"Folate metabolism, Histidine metabolism","added, present","Glutamate formiminotransferase deficiency"]
[null,"khk","reduced",0.97,null,"Fructose and mannose metabolism","present","[Fructosuria]"]
[null,"si","reduced",0.97,null,"Galactose metabolism, Starch and sucrose metabolism","added, present","Sucrase-isomaltase deficiency, congenital"]
[null,"si:ch73-71d17.1","reduced",0.97,null,"Histidine metabolism","present"]
[null,"uroc1","reduced",0.97,null,"Histidine metabolism","present","?Urocanase deficiency"]
[null,"phgdh","reduced",0.977,null,"Glycine, serine, alanine and threonine metabolism","present","Neu-Laxova syndrome 1; Phosphoglycerate dehydrogenase deficiency"]
[null,"psat1","reduced",0.977,null,"Glycine, serine, alanine and threonine metabolism, Vitamin B6 metabolism","present","Neu-Laxova syndrome 2; ?Phosphoserine aminotransferase deficiency"]
[null,"psph","reduced",0.977,null,"Glycine, serine, alanine and threonine metabolism","present","Phosphoserine phosphatase deficiency"]
[null,"aco2","reduced",0.978,null,"Citric acid cycle","present","Infantile cerebellar-retinal degeneration; ?Optic atrophy 9 "]
[null,"idh2","reduced",0.978,null,"Citric acid cycle, Miscellaneous","present","D-2-hydroxyglutaric aciduria 2"]
[null,"bcat2","reduced",0.979,null,"Valine, leucine, and isoleucine metabolism","present","?Hypervalinemia or hyperleucine-isoleucinemia"]
[null,"mthfd1b","reduced",0.983,null,"Folate metabolism","present","Combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia; {Neural tube defects, folate-sensitive, susceptibility to}"]
[null,"mthfd2","reduced",0.983,null,"Folate metabolism","added, present"]
[null,"rpe","reduced",0.987,"*","Pentose phosphate pathway","present"]
[null,"taldo1","reduced",0.987,null,"Pentose phosphate pathway","present","Transaldolase deficiency"]
[null,"oat","reduced",0.993,null,"Urea cycle","present","Gyrate atrophy of choroid and retina with or without ornithinemia"]
[null,"aldh4a1","reduced",0.994,null,"Arginine and Proline Metabolism, Glutamate metabolism, Urea cycle","present","Hyperprolinemia, type II"]
[null,"shmt1","reduced",0.994,null,"Glycine, serine, alanine and threonine metabolism","present"]
[null,"aacs","reduced",0.996,null,"Propanoate metabolism","present"]
[null,"fah","reduced",0.996,null,"Tyrosine metabolism","present","Tyrosinemia, type I"]
[null,"got1","reduced",0.996,null,"Methionine and cysteine metabolism, Phenylalanine metabolism, Arginine and Proline Metabolism, Alanine and aspartate metabolism, Tyrosine metabolism","present","Aspartate aminotransferase, serum level of, QTL1"]
[null,"gstz1","reduced",0.996,null,"Tyrosine metabolism","present","[Maleylacetoacetate isomerase deficiency]"]
[null,"hgd","reduced",0.996,null,"Tyrosine metabolism","present","Alkaptonuria"]
[null,"lpl","reduced",0.996,null,"Triacylglycerol synthesis","present","Combined hyperlipidemia, familial; [High density lipoprotein cholesterol level QTL 11]; Lipoprotein lipase deficiency"]
[null,"sod2","reduced",0.998,"*","ROS detoxification","added","{Microvascular complications of diabetes 6}"]
[null,"COX1","reduced",0.998,null,"Oxidative phosphorylation","added"]
[null,"COX2","reduced",0.998,null,"Oxidative phosphorylation","added"]
[null,"COX3","reduced",0.998,null,"Oxidative phosphorylation","added"]
[null,"cox4i2","reduced",0.998,null,"Oxidative phosphorylation","added","Exocrine pancreatic insufficiency, dyserythropoietic anemia, and calvarial hyperostosis"]
[null,"cox6b2","reduced",0.998,null,"Oxidative phosphorylation","added"]
[null,"cox7a1","reduced",0.998,null,"Oxidative phosphorylation","added"]
[null,"gnmt","reduced",0.999,null,"Glycine, serine, alanine and threonine metabolism","present","Glycine N-methyltransferase deficiency"]
[null,"ldhd","reduced",0.999,null,"Pyruvate metabolism","added, present"]
[null,"sardh","reduced",0.999,null,"Urea cycle","present","[Sarcosinemia]"]
[null,"slc16a1b","reduced",0.999,null,"Transport, mitochondrial, Transport, extracellular","present","Erythrocyte lactate transporter defect; Hyperinsulinemic hypoglycemia, familial, 7; Monocarboxylate transporter 1 deficiency"]
[null,"bdh2","reduced",1,"*","Butanoate metabolism","present"]
[null,"aqp8a.1","reduced",1,null,"Transport, mitochondrial","present"]
[null,"bdh1","reduced",1,null,"Butanoate metabolism","present"]
[]
[null,"Normal growth, mentioned in genetic screens",{"$nulls":4},"Reactions statuses ZebraGEM 2.0","Remark"]
[null,"adssl","normal",1,"1,2,3,*","Nucleotide interconversion","present","At expense of biomass"]
[null,"atp1a1a.1","normal",1,"1,4,*","Transport, extracellular","present","Blocked reaction"]
[null,"atp6ap1b","normal",1,"1,3,*","Transport, lysosomal","present","At expense of biomass"]
[null,"atp6v0ca","normal",1,"1,2,*","Transport, lysosomal","present","At expense of biomass"]
[null,"atp6v0d1","normal",1,"1,*","Transport, lysosomal","present","At expense of biomass"]
[null,"atp6v1e1b","normal",1,"1,2","Transport, lysosomal","present","At expense of biomass"]
[null,"atp6v1f","normal",1,"1,*","Transport, lysosomal","present","At expense of biomass"]
[null,"atp6v1h","normal",1,"1,2,*","Transport, lysosomal","present","At expense of biomass"]
[null,"b3gat3","normal",1,"1,2","Chondroitin synthesis","present","Blocked reaction"]
[null,"b4galt7","normal",1,1,"Chondroitin synthesis","present","Blocked reaction"]
[null,"dlat","normal",1,5,"Glycolysis/gluconeogenesis, Miscellaneous","present","At expense of biomass"]
[null,"dlst","normal",1,"1,*","Lysine metabolism, Miscellaneous, Citric acid cycle","present","At expense of biomass"]
[null,"dnmt1","normal",1,"8,*","Methionine and cysteine metabolism","present","Blocked reaction"]
[null,"fech","normal",1,"4,*","Heme synthesis","present","Blocked reaction"]
[null,"kars","normal",1,1,"Lysine metabolism","present","Blocked reaction"]
[null,"lclat1","normal",1,"4,*","Glycerophospholipid metabolism","present","Blocked reaction"]
[null,"LOC100330864","normal",1,2,"Glutathione metabolism, Nucleotide interconversion","present","Unknown"]
[null,"mars","normal",1,"1,*","Methionine and cysteine metabolism, Selenoamino acid metabolism","present","Blocked reaction"]
[null,"odc1","normal",1,"1,2","Urea cycle","present","Blocked reaction"]
[null,"plcg1","normal",1,"1,7,*","Inositol phosphate metabolism","present","At expense of biomass"]
[null,"qars","normal",1,1,"Glutamate metabolism","present","Blocked reaction"]
[null,"rrm2","normal",1,"1,2,3","Glutathione metabolism, Nucleotide interconversion","present","Redundant"]
[null,"sars","normal",1,"1,*","Glycine, serine, alanine and threonine metabolism","present","Blocked reaction"]
[null,"slc25a5","normal",1,"1,2","Transport, mitochondrial","present","Redundant"]
[null,"slc35d1a","normal",1,1,"Transport, endoplasmic reticular","present","Blocked reaction"]
[null,"synj1","normal",1,6,"Inositol phosphate metabolism","present","Unknown"]
[null,"ugdh","normal",1,"4,*","Starch and sucrose metabolism","present","Blocked reaction"]
[null,"uxs1","normal",1,"1,2","Nucleotide sugar metabolism","present","Blocked reaction"]
[null,"vars","normal",1,"1,2","Valine, leucine, and isoleucine metabolism","present","Blocked reaction"]
[]
[]
[]
["B) Results of double knock-out experiments. First listed are gene pairs with genes without a single KO phenotype in the model. Second are gene pairs with at least one gene with a non-lethal single KO phenotype that result in lethal double Kos. Bold faced genes do appear in the monogenetic knockdown abnormal phenotype search in ZFIN. Italic genes are found in the genetic screens."]
["Double knockouts without single KO phenotype"]
["Gene 1","Gene 2","Phenotype","Growth rate fraction",null,"subsystems","Reactions statuses ZebraGEM 2.0"]
["rrm2","LOC100330864","lethal",0,null,"Glutathione metabolism, Nucleotide interconversion","present"]
["cbsb","cbsa","lethal",0,null,"Glycine, serine, alanine and threonine metabolism, Methionine and cysteine metabolism","present"]
["suclg2","suclg1","lethal",0,null,"Citric acid cycle","present"]
["adkb","adka","lethal",0,null,"Nucleotide interconversion","present"]
["cox5ab","cox5aa","lethal",0,null,"Oxidative phosphorylation","added"]
["degs1","degs2","lethal",0,null,"Sphingolipid metabolism","added, present"]
["nmnat2","si:ch211-168k14.2","lethal",0,null,"Glycerophospholipid metabolism, NAD metabolism","added, present"]
["ggps1","fdps","lethal",0,null,"Cholesterol metabolism","present"]
["guk1b","guk1a","lethal",0,null,"Nucleotide interconversion","present"]
["ptdss1a","pcyt2","lethal",0,null,"Glycerophospholipid metabolism","present"]
["ptdss1a","ptdss2","lethal",0,null,"Glycerophospholipid metabolism","present"]
["gpt2l","si:ch211-217a12.1","lethal",0,null,"Glutamate metabolism","present"]
["pccb","pcca","lethal",0,null,"Valine, leucine, and isoleucine metabolism","present"]
["msmo1","faxdc2","lethal",0,null,"Cholesterol metabolism","present"]
["sdhda","sdhdb","lethal",0,null,"Citric acid cycle","present"]
["coasy","si:ch211-168k14.2","lethal",0,null,"CoA synthesis, Glycerophospholipid metabolism","present"]
["cratb","crata","lethal",0,null,"Fatty acid oxidation","added"]
["hmgcrb","hmgcra","lethal",0,null,"Squalene and cholesterol synthesis","present"]
["sptlc2a","sptlc2b","lethal",0,null,"Sphingolipid metabolism","present"]
["cox7a2a","cox7a2b","lethal",0,null,"Oxidative phosphorylation","added"]
["slc16a10","slc6a14","lethal",0,null,"Transport, extracellular","present"]
["cox5b2","cox5b1","lethal",0,null,"Oxidative phosphorylation","added"]
["atp5ia","atp5ib","reduced",0.509427237495741,null,"Oxidative phosphorylation","added"]
["atp5j2","atp5j","reduced",0.50942723749686,null,"Oxidative phosphorylation","added"]
["atp5b","zgc:163069","reduced",0.50942723749686,null,"Oxidative phosphorylation","added"]
["zgc:163069","atp5b","reduced",0.50942723749686,null,"Oxidative phosphorylation","added"]
["gapdh","gapdhs","reduced",0.646627246934422,null,"Glycolysis/gluconeogenesis","present"]
["tpi1a","tpi1b","reduced",0.672514098656466,null,"Glycolysis/gluconeogenesis","present"]
["gad1b","gad2","reduced",0.716969497866551,null,"Glutamate metabolism, Taurine and hypotaurine metabolism, beta-Alanine metabolism","present"]
["pgm1","pgm2","reduced",0.744046060187671,null,"Glycolysis/gluconeogenesis, Pentose phosphate pathway","present"]
["prps1a","prps1b","reduced",0.756006026869058,null,"Pentose phosphate pathway","present"]
["nme6","nme4","reduced",0.818004901322838,null,"Nucleotide interconversion","present"]
["slc25a12","mdh2","reduced",0.831099302946357,null,"Transport, mitochondrial, Citric acid cycle","present"]
["chka","chkb","reduced",0.872395369185018,null,"Glycerophospholipid metabolism","present"]
["acat2","acat1","reduced",0.912773379714914,null,"Fatty acid oxidation, Valine, leucine, and isoleucine metabolism, Tryptophan metabolism","present"]
["ckma","ckbb","reduced",0.917686405300154,null,"Urea cycle","present"]
["ckmt1","ckmt2b","reduced",0.917686405300154,null,"Urea cycle","present"]
["si:dkey-180p18.9","akr1a1b","reduced",0.969725800961074,null,"Pyruvate metabolism, Galactose metabolism, Glycolysis/gluconeogenesis, Pentose phosphate pathway, Glycerophospholipid metabolism, Fructose and mannose metabolism","added, present"]
["slc2a11a","slc5a9","reduced",0.969725800961074,null,"Transport, extracellular","present"]
["cat","prdx6","reduced",0.973662246814981,null,"Phenylalanine metabolism, ROS detoxification, Glutathione metabolism, Tryptophan metabolism, Miscellaneous","added, present"]
["lipf","rpe65c","reduced",0.984643130323382,null,"Steroid metabolism, Vitamin A metabolism, Transport, extracellular","added, present"]
["lcat","rpe65c","reduced",0.984643130323382,null,"Vitamin A metabolism, Fatty acid oxidation","added, present"]
["tktb","tkta","reduced",0.986885675685786,null,"Pentose phosphate pathway","present"]
["hpda","hpdb","reduced",0.996008598385582,null,"Phenylalanine metabolism, Tyrosine metabolism","present"]
[]
["Lethal double KO with non-lethal genes"]
["Gene 1 ","Gene 2","Phenotype","Growth rate fraction",null,"Subsystems","Reactions statuses ZebraGEM 2.0"]
["mthfd1b","mthfd1l","lethal",0,null,"Folate metabolism","added, present"]
["phgdh","shmt1","lethal",0,null,"Glycine, serine, alanine and threonine metabolism","present"]
["cs","ldhd","lethal",0,null,"Pyruvate metabolism, Citric acid cycle","added, present"]
["cs","gcat","lethal",0,null,"Glycine, serine, alanine and threonine metabolism, Citric acid cycle","present"]
["cs","hagh","lethal",0,null,"Pyruvate metabolism, Citric acid cycle","present"]
["cs","mao","lethal",0,null,"Phenylalanine metabolism, Arginine and Proline Metabolism, Citric acid cycle, Glycine, serine, alanine and threonine metabolism, Tyrosine metabolism, Tryptophan metabolism, Miscellaneous, Histidine metabolism","present"]
["cs","glo1","lethal",0,null,"Pyruvate metabolism, Citric acid cycle","present"]
["taldo1","pgd","lethal",0,null,"Pentose phosphate pathway","present"]
["taldo1","pgls","lethal",0,null,"Pentose phosphate pathway","present"]
["taldo1","g6pd","lethal",0,null,"Pentose phosphate pathway","present"]
["psat1","shmt1","lethal",0,null,"Glycine, serine, alanine and threonine metabolism, Vitamin B6 metabolism","present"]
["rpe","pgd","lethal",0,null,"Pentose phosphate pathway","present"]
["rpe","pgls","lethal",0,null,"Pentose phosphate pathway","present"]
["rpe","g6pd","lethal",0,null,"Pentose phosphate pathway","present"]
["aacs","hmgcs1","lethal",0,null,"Cholesterol metabolism, Propanoate metabolism","present"]
["shmt1","psph","lethal",0,null,"Glycine, serine, alanine and threonine metabolism","present"]
["hmgcs1","bckdhb","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism, Propanoate metabolism","added, present"]
["hmgcs1","bckdha","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism, Propanoate metabolism","added, present"]
["hmgcs1","auh","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism","present"]
["hmgcs1","dbt","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism, Propanoate metabolism","added, present"]
["hmgcs1","dldh","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Lysine metabolism, Citric acid cycle, Propanoate metabolism, Glycine, serine, alanine and threonine metabolism, Glycolysis/gluconeogenesis, Cholesterol metabolism","added, present"]
["hmgcs1","mccc1","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism","present"]
["hmgcs1","mccc2","lethal",0,null,"Valine, leucine, and isoleucine metabolism, Cholesterol metabolism","present"]
["gnmt","pemt","lethal",0,null,"Glycine, serine, alanine and threonine metabolism, Glycerophospholipid metabolism","present"]
["got1","slc25a12","lethal",0,null,"Methionine and cysteine metabolism, Phenylalanine metabolism, Alanine and aspartate metabolism, Transport, mitochondrial, Tyrosine metabolism, Arginine and Proline Metabolism","present"]
["cept1b","mtr","lethal",0,null,"Methionine and cysteine metabolism, Selenoamino acid metabolism, Glycerophospholipid metabolism","present"]
["cept1b","mthfr","lethal",0,null,"Glycerophospholipid metabolism, Folate metabolism","present"]
["cept1b","pcyt2","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cept1b","pemt","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cept1b","si:ch211-197l9.4","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cept1b","ptdss2","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cds1","cmpk2","lethal",0,null,"Glycerophospholipid metabolism, Nucleotide interconversion","present"]
["cds1","cds2","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cds1","pemt","lethal",0,null,"Glycerophospholipid metabolism","present"]
["cds1","slc29a1a","lethal",0,null,"Transport, mitochondrial, Glycerophospholipid metabolism, Transport, extracellular","present"]
["cds1","slc25a26","lethal",0,null,"Transport, mitochondrial, Glycerophospholipid metabolism","present"]