This PDF file includes:

• Fig. S1. Disordered motif of U1-70K predicted by different models.
• Fig. S2. Statistics of average sizes of droplets of LC1 and LC2.
• Fig. S3. FRAP of the irregular droplets.
• Fig. S4. Images showing effects of high salt (500 mM NaCl) to LC2 LLPS.
• Fig. S5. Characterization of aggregation and secondary structure of RD19.
• Fig. S6. The effects of RNA to the LLPS of LC1 and LC2.
• Fig. S7. TEM images of aggregates formed by LC1 and LC2 in solution and in droplets.
• Table S1. Densities of droplets of LC1 and LC2 under various pH values or with different amino acids added.
• Table S2. Primers used for gene construction.
• Table S3. Parameters of LC1, LC2, and LC predicted by CIDAR.

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