O-GlcNAcylation of Thr¹²/Ser⁵⁶ in short-form O-GlcNAc transferase (sOGT) regulates its substrate selectivity

Supporting Information

• Supporting Information (to be published online) - supporting figures, table, methods
• Supplementary mass spectra - Manually confirm O-GlcNAc Mass spectra of peptides with a score ≥ 300
• sheet S1 - Peptides in ETD-MS/MS analysis
• sheet-S2 - Total proteins identified in O-GlcNAc proteomic study
• sheet-S3 - Differential proteins between T12A and wild type sOGT
• sheet S4 - Differential proteins between S56A and wild type sOGT
• sheet S5 - Manually confirm O-GlcNAc peptides with a score ≥ 300
• sheet S6 - Putative O-GlcNAc proteins identified in this work (previously reported in literatures). O-GlcNAc proteins were checked in: p: dbPTM (http://dbptm.mbc.nctu.edu.tw/browse_showPTM.php?type=O-linked Glycosylation&AA=S); u: Uniprot (2018_uniprot_sp_glyco_database_sites) ; *: validated in MS data or western blot in this paper; Literatures L1(1), L2(2), L3(3), L4(4).