The PDFset includes:

• Fig. S1. Topological loading of yeast cohesin on plasmid DNA.
• Fig. S2. Analysis of yeast cohesin on DNA curtains.
• Fig. S3. Intramolecular cohesin bridging requires ATP.
• Fig. S4. Purification of budding yeast cohesin ATPase mutant.
• Fig. S5. Permanent cohesin bridges are not displaced by physical stretching of λ-DNA.
• Fig. S6. Cohesin does not capture two λ-DNAs in sequential steps.
• Fig. S7. DNA friction experiments confirm the presence of cohesin complexes on extended λ-DNA.
• Fig. S8. Generation of permanent cohesin bridges using a quadrupole-trap optical tweezer.
• Fig. S9. Purification of human cohesin and yeast condensin.
• Fig. S10. Budding yeast condensin, but not cohesin, compacts λ-DNA against 1 pN stretching force.
• Legends for tables S1 and S2
• Legends for movies S1 to S6

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Mass spectrometry analysis of cohesin wild type and ATPase mutant (Smc3-K38I) tetramer complexes and the loader complex Scc2-Scc4.
• Table S2 (Microsoft Excel format). Mass spectrometry analysis of cohesin ATPase mutant (Smc3-K38I) tetramer peptides showing peptides containing the K38I mutation for SMC3.
• Movie S1 (.mp4 format). Time-lapse videos showing cohesin tethering.
• Movie S2 (.mp4 format). Time-lapse videos showing cohesin tethering.
• Movie S3 (.mp4 format). Time-lapse videos showing cohesin tethering.
• Movie S4 (.mp4 format). Time-lapse videos showing sliding of intermolecular bridges in a quadruple-trap optical tweezer.
• Movie S5 (.mp4 format). Time-lapse videos showing sliding of intermolecular bridges in a quadruple-trap optical tweezer.
• Movie S6 (.mp4 format). Time-lapse video showing pulling on intermolecular bridges in a quadruple-trap optical tweezer.