FungiAgaricalesEntolomataceaeHeXiao-LanHorakEgonWangDiLiTai-HuiPengWei-HongGanBing-ChengDescriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l.MycoKeys051220196112610.3897/mycokeys.61.46446B2AA2990-48FB-56A3-A3C6-2F396B4F8804 Entoloma byssisedum var. microsporum Esteve-Rav. & Noordel.Figures 1l, m, 7Description of Chinese material.

Pileus 5–20 mm, reniform, broadly convex, expanding to applanate, whitish-grey to greyish, entirely matted-tomentose to matted-appressed fibrillose, fibrils whitish, slightly hygrophanous, not striate. Lamellae with 2–3 tiers of lamellulae, adnexed, ventricose, up to 2.5 mm wide, moderately close, pale greyish at first, becoming greyish-pink, entire margin concolorous. Stipe 1–5 × 0.5–1 mm, strongly reduced, lateral, grey, covered with minutely, pale greyish fibrils, at base with white hairy mycelium. Basal rhizoids present, white. Context thin, unchanging. Odour absent. Taste not distinctive.

Basidiospores 8–10 × 5.5–7 (7.5) µm (x = 9 ± 0.3 × 6.5 ± 0.2 µm), Q = 1.29–1.52, Q = 1.39 ± 0.04, 5–6 (7)-angled, heterodiametric in profile view. Basidia 30–34 × 9–11 µm, clavate, 4-spored, rarely 2-spored, clampless. Lamellar edge fertile. Cheilocystidia, pleurocystidia and caulocystidia absent. Pileipellis a cutis composed of cylindrical hyphae, repent terminal cells (30–) 35–50 × 4–7 µm, cylindrical (or slender subclavate), non-gelatinised wall thin, smooth or minutely encrusted with pale brown pigment. Oleiferous hyphae numerous in pileipellis. Clamp-connections present in the pileipellis.

Habitat.

On decaying stump of Betula sp. in deciduous forest dominated by Betula sp. and Quercus sp.

Materials examined.

China. Sichuan Prov.: Kangding County, Mugecuo, ca. 2700 m elev., 30°13'N, 101°83'E, on decaying stump of Betula sp., 4 August 2015, X.L. He (SAAS 1160); on decaying stump of Betula sp., 3 September 2015, X.L. He (SAAS 1828); on decaying stump of Betula sp., 3 September 2015, X.L. He (SAAS 1279; ZT 13608). Xizang Autonomous Region (Tibet): Linzhi County, Kadinggou, ca. 2980 m elev., 29°50'N, 93°25'E, on decaying stump of Betula sp., 25 September 2014, X.L. He (SAAS 1025); Linzhi County, Sejila Mountain, ca. 3600 m elev., 29°35'N, 94°25'E, on decaying stump of Betula sp., 24 September 2014, X.L. He (SAAS 1953).

Remarks.

Entoloma byssisedum var. microsporum closely resembles typical E. byssisedum by its small crepidotoid pale greyish-brown basidiomes whose pileipellis is covered with fine, whitish arachnoid fibrils and lateral, strongly reduced to absent stipe. However, the basidiospores of the Chinese specimens are distinctly smaller as recorded for typical E. byssisedum and, thus, the morphotaxonomic characters correspond well with European collections of E. byssisedum var. microsporum (Noordeloos 2004). The identification is further supported by ITS sequences which demonstrate that the Chinese specimens of E. byssisedum var. microsporum are 99% identical as compared to those reported for European material (KJ001409). It is noteworthy that, in GenBank, there are two sequences labelled “E. byssisedum” (EU784209, KJ001413) which, however, are significantly different. The ITS sequences of the Chinese E. byssisedum var. microsporum are 97% identical to EU784209 but do not correspond with KJ001413. Accordingly, it could be speculated that E. byssisedum var. microsporum and E. byssisedum var. byssisedum actually represent two different species.

10.3897/mycokeys.61.46446.figure7080CC3E4-087F-5AE5-87E7-52D869AE5EAD

Microscopic structures of Entoloma byssisedum var. microsporum (SAAS 1279) a Basidiospores b Basidia c Pileipellis.

https://binary.pensoft.net/fig/362626
10.3897/mycokeys.61.46446.figure1ED1EED75-DEB7-5613-AE51-99BC9FB18590

Basidiomes of Claudopus species a Basidiomes of E. conchatum on soil (SAAS 1712) b Basidiomes of E. conchatum on stem of live Pinus (SAAS 1014) c Pileus of E. flabellatum (SAAS 1501) d Lamellae of E. flabellatum (SAAS 1080) e Basidiomes of C. gregarious on bark-wood of live Castanopsis (SAAS 1220) f Red droplets on the lamellar edges of E. gregarium (SAAS 1493) g Basidiomes of E. pleurotoides on decaying bark-wood of Castanopsis (SAAS 1215) h Basidiomes of E. pleurotoides on bark-wood of live Castanopsis (SAAS 1252) i Basidiomes of E. reductum on decaying stump of Castanopsis (holotype, SAAS 1091) j Mature basidiomes of E. reductum on rock (SAAS 2068) k Young basidiomes of E. reductum on soil (SAAS 1016) l Lamellae of E. byssisedum var. microsporum (SAAS 1828) m Basidiomes of E. byssisedum var. microsporum on decaying stump of Betula (SAAS 1160).

https://binary.pensoft.net/fig/362620
NoordeloosME (2004) Entoloma s.l. Fungi Europaei, vol. 5a. Edizione Candusso, Italy.