LOCUS Exported 15489 bp ds-DNA circular SYN 10-AUG-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS S2 File SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15489) AUTHORS Haider Sami TITLE Direct Submission JOURNAL Exported Friday, Aug 10, 2018 from SnapGene 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15489 /organism="recombinant plasmid" /mol_type="other DNA" LTR 94..327 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 399..520 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 539..674 /note="SV40 ori" /note="SV40 origin of replication" promoter complement(707..725) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(735..751) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 896..1351 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1380..1484 /gene="bla" /note="AmpR promoter" CDS 1485..2345 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2516..3104 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 3392..3413 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 3428..3458 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 3466..3482 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3490..3506 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 3527..3545 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" LTR 3801..3981 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 4025..4150 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 4643..4876 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 5365..5482 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 5537..5561 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB1" /note="recombination site for the Gateway(R) BP reaction" enhancer 5589..5968 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 5969..6172 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 6217..6235 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 6277..6996 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" protein_bind 7037..7057 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB5" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 7091..7101 /note="TCF binding site" misc_feature 7106..7116 /note="TCF binding site" misc_feature 7121..7131 /note="TCF binding site" misc_feature 7137..7147 /note="TCF binding site" misc_feature 7152..7162 /note="TCF binding site" misc_feature 7167..7177 /note="TCF binding site" misc_feature 7182..7192 /note="TCF binding site" promoter 7232..7263 /note="minP" /note="minimal TATA-box promoter with low basal activity" misc_feature 7291..7295 /note="MCS" /note="multiple cloning site" CDS 7325..7840 /codon_start=1 /product="NanoLuc(R) luciferase" /note="Nluc" /note="human codon-optimized" /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILA" polyA_signal 7880..8101 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" protein_bind complement(8200..8220) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB4" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 8247..8287 /note="bom" /note="basis of mobility region from pBR322" polyA_signal 8526..8660 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" polyA_signal 8754..8888 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" misc_feature 8922..8960 /note="3xGLIBS" /note="Identified in JM94 2017-11-21, sequence was given by collaborator, see lab book 17.8.17." misc_feature 8961..8999 /note="3xGLIBS" /note="Identified in JM94 2017-11-21, sequence was given by collaborator, see lab book 17.8.17." misc_feature 9000..9038 /note="3xGLIBS" /note="Identified in JM94 2017-11-21, sequence was given by collaborator, see lab book 17.8.17." promoter 9129..9191 /note="Mini-TK promoter" /note="minimal herpes simplex virus (HSV) thymidine kinase promoter" misc_feature 9247..10900 /note="Luciferase, manually added, JM94 2017-11-21" intron 11032..11097 /note="small t intron" /note="simian virus 40 (SV40) small t antigen intron" CDS 11227..11247 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" polyA_signal 11672..11806 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" protein_bind 12358..12378 /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB3" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 12459..12617 /note="CBF binding site (from depositor's map)" /note="CBF binding sites" misc_feature 12460..12531 /note="4 CBF binding elements (from depositor's map)" /note="Sequence of each element: GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG JM" misc_feature 12492..12497 /note="CBF1 binding site" /note="Presumptive first CBF binding site GTGGGAA" misc_feature 12527..12531 /note="CBF1 binding site" misc_feature 12562..12567 /note="CBF1 binding site" misc_feature 12597..12602 /note="CBF1 binding site" promoter 12618..12814 /note="SV40 promoter" /note="SV40 early promoter" rep_origin 12665..12800 /note="SV40 ori" /note="SV40 origin of replication" gene 12883..13440 /gene="GLuc" /label=GLuc /note="GLuc" CDS 12883..13440 /codon_start=1 /gene="GLuc" /product="Gaussia princeps luciferase" /label=Gaussia princeps luciferase /note="GLuc" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ RCATFASKIQGQVDKIKGAGGD" sig_peptide 12883..12933 /gene="GLuc" /label=GLuc(1) /note="GLuc(1)" /note="secretory signal sequence" protein_bind complement(13469..13493) /gene="mutant version of attB" /bound_moiety="BP Clonase(TM)" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" misc_feature 13524..14112 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(13995..14006) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" promoter 14126..14625 /note="PGK promoter" /note="mouse phosphoglycerate kinase 1 promoter" CDS 14667..15461 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgcgtatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag 61 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa 121 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 301 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 361 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 601 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 661 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg 721 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 781 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 841 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg 901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 1021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 1081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 1141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 1201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 1261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 1321 aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc 1381 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 1441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 1501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 1561 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 1621 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 1681 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 1741 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 1801 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 1861 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 1921 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 1981 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 2041 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 2101 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 2161 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 2221 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 2281 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 2341 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 2401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 2461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 3241 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 3301 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 3361 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 3421 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 3481 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact 3541 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc 3601 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc 3661 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg 3721 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg 3781 cctagctcga tacaataaac gggtctctct ggttagacca gatctgagcc tgggagctct 3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc 4021 agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg 4081 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg 4141 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag 4201 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga 4261 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg 4321 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt 4381 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga 4441 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct 4501 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag 4561 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga 4621 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa 4681 gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta 4741 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac 4801 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa 4861 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg 4921 tgccttggaa tgctagttgg agtaataaat ctctggaaca gattggaatc acacgacctg 4981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga 5041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag 5101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat 5161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt 5221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga 5281 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt 5341 agtgaacgga tctcgacggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc 5401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca 5461 aattacaaaa attcaaaatt ttattccagt gtggtggaat tgagtattcc agtgtggtgg 5521 aattctgcag atatcaacaa gtttgtacaa aaaagcaggc tgcgatgtac gggccagata 5581 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 5641 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 5701 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 5761 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 5821 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 5881 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 5941 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 6001 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 6061 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 6121 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct 6181 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac 6241 ccaagcttgg taccgagctc ggatcctaaa gccaccatgg tgagcaaggg cgaggagctg 6301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 6361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 6421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 6481 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 6541 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 6601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 6661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 6721 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 6781 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 6841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 6901 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 6961 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagagctcga 7021 gcatgcatct agaggacaac tttgtataca aaagttggga gacccaagct tggtaccgag 7081 ctcttacgcg agatcaaagg gggtaagatc aaagggggta agatcaaagg ggcgcgagat 7141 caaagggggt aagatcaaag ggggtaagat caaagggggt aagatcaaag gggcgcgccc 7201 gcgtgctagc ccgggctcga gatctagact ctagagggta tataatggaa gctcgaattc 7261 cagcttggca ttccggtact gttggtaaaa agcttggcaa tccggtactg ttggtaaagc 7321 caccatggtc ttcacactcg aagatttcgt tggggactgg cgacagacag ccggctacaa 7381 cctggaccaa gtccttgaac agggaggtgt gtccagtttg tttcagaatc tcggggtgtc 7441 cgtaactccg atccaaagga ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca 7501 tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt 7561 taaggtggtg taccctgtgg atgatcatca ctttaaggtg atcctgcact atggcacact 7621 ggtaatcgac ggggttacgc cgaacatgat cgactatttc ggacggccgt atgaaggcat 7681 cgccgtgttc gacggcaaaa agatcactgt aacagggacc ctgtggaacg gcaacaaaat 7741 tatcgacgag cgcctgatca accccgacgg ctccctgctg ttccgagtaa ccatcaacgg 7801 agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa ggccgcgact ctagagtcgg 7861 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca 7921 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 7981 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 8041 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 8101 aaaatcgata aggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca 8161 tcacactggc ggccgctcga gcatgcatct agaggcaccc aacttttcta tacaaagttg 8221 ggagacccaa gcttggtacc gagctctata cactccgcta tcgctacgtg actgggtcat 8281 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 8341 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 8401 accgtcatca ccgaaacgcg cgaggcagga tcagccatat cacatttgta gaggttttac 8461 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 8521 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8641 tatcttatca tgtctggatc agccatatca catttgtaga ggttttactt gctttaaaaa 8701 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttaacttgt 8761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 8821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 8881 tctggatcca agctcagatc caagcttatc gataccgtcg agtgggtggt ctgggtggtc 8941 tgggtggtct gggtggtcga gtgggtggtc tgggtggtct gggtggtctg ggtggtcgag 9001 tgggtggtct gggtggtctg ggtggtctgg gtggtcgacc tcgagatccg gcaaaccccg 9061 cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg 9121 aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc 9181 gacccgctta acagcgtcaa cagcgtgccg cagatctgag cttggcattc cggtactgtt 9241 ggtaaaatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag 9301 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga 9361 acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa 9421 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc 9481 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc 9541 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg 9601 aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac 9661 gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa aacggattac 9721 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa 9781 tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc 9841 tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga 9901 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta 9961 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt 10021 ggatttcgag tcgtcttaat gtatagattt gaagaaaagc tgtttttacg atcccttcag 10081 gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc 10141 actctgattg anaaatacga tttatctaat ttacacgaaa ttgcttctgg ggggcgcacc 10201 tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc catcttccag ggatacgaca 10261 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa 10321 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac 10381 cgggaaaacg ctgggcgtta atcagagagg cgaattatgt gtcagaggac ctatgattat 10441 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct 10501 acattctgga gacatagctt actgggacga agacgaacac ttcttcatag ttgaccgctt 10561 gaagtcttta attaaataca aaggatatca ggtggccccc gctgaattgg aatcgatatt 10621 gttacaacac cccaacatct tcgacgcggg cgtggcaggt cttcccgacg atgacgccgg 10681 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat 10741 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt 10801 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat 10861 cctcataaag gccaagaagg gcggaaagtc caaattgtaa aatgtaactg tattcagcga 10921 tgacgaaatt cttagctatt gtaatgactc tagaggatct ttgtgaagga accttacttc 10981 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata 11041 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt 11101 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg 11161 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct 11221 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta 11281 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc 11341 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt 11401 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat 11461 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt 11521 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca 11581 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 11641 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 11701 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 11761 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc cccaggaagc 11821 tcctctgtgt cctcataaac cctaacctcc tctacttgag aggacattcc aatcataggc 11881 tgcccatcca ccctctgtgt cctcctgtta attaggtcac ttaacaaaaa ggaaattggg 11941 taggggtttt tcacagaccg ctttctaagg gtaattttaa aatatctggg aagtcccttc 12001 cactgctgtg ttccagaagt gttggtaaac agcccacaaa tgtcaacagc agaaacatac 12061 aagctgtcag ctttgcacaa gggcccaaca ccctgctcat caagaagcac tgtggttgct 12121 gtgttagtaa tgtgcaaaac aggaggcaca ttttccccac ctgtgtaggt tccaaaatat 12181 ctagtgtttt catttttact tggatcagga acccagcact ccactggata agcattatcc 12241 ttatccaaaa cagccttgtg gtcagtgttc atctgctgac tagtaacggc cgccagtgtg 12301 ctggaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagaggacaa 12361 ctttgtataa taaagttggg agacccaagc ttggtaccga gctcggatcc actagtaacg 12421 gccgccagtg tgctggaatt cgtattaccg ccatgcatta gttattaatc gagatctggt 12481 gtaaacacgc cgtgggaaaa aatttatgga tctggagtaa cacgccgtgg gaaaaaattt 12541 atggatctgg agtaacacgc cgtgggaaaa aatttatgga tctggagtaa cacgccgtgg 12601 gaaaaaattt atggatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 12661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 12721 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 12781 aggaggcttt tttggaggcc taggcttttg caaaaagctg gctagcgcta ccggactcag 12841 atctcgagct caagcttggt accgagctcg gatccagcca ccatgggagt caaagttctg 12901 tttgccctga tctgcatcgc tgtggccgag gccaagccca ccgagaacaa cgaagacttc 12961 aacatcgtgg ccgtggccag caacttcgcg accacggatc tcgatgctga ccgcgggaag 13021 ttgcccggca agaagctgcc gctggaggtg ctcaaagaga tggaagccaa tgcccggaaa 13081 gctggctgca ccaggggctg tctgatctgc ctgtcccaca tcaagtgcac gcccaagatg 13141 aagaagttca tcccaggacg ctgccacacc tacgaaggcg acaaagagtc cgcacagggc 13201 ggcataggcg aggcgatcgt cgacattcct gagattcctg ggttcaagga cttggagccc 13261 atggagcagt tcatcgcaca ggtcgatctg tgtgtggact gcacaactgg ctgcctcaaa 13321 gggcttgcca acgtgcagtg ttctgacctg ctcaagaagt ggctgccgca acgctgtgcg 13381 acctttgcca gcaagatcca gggccaggtg gacaagatca agggggccgg tggtgactaa 13441 gcggccgctc gagcatgcat ctagaggtac ccagctttct tgtacaaagt ggttgatatc 13501 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga 13561 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 13621 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 13681 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 13741 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 13801 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 13861 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 13921 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 13981 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 14041 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 14101 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga 14161 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc 14221 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg 14281 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc 14341 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct 14401 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct 14461 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg 14521 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc 14581 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc 14641 ttaccacact cctgcaggcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 14701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 14761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 14821 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 14881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 14941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 15001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 15061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 15121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 15181 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 15241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 15301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 15361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 15421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 15481 aatgaccga //