The PDFset includes:

• Fig. S1. Genetic deletion of Mtif3 in mice (related to Fig. 1).
• Fig. S2. mtRNA abundance is perturbed in the absence of MTIF3 (related to Fig. 3).
• Fig. S3. The relative abundance of OXPHOS steady-state proteins levels in control and Mtif3 knockout mouse mitochondria (related to Fig. 3).
• Fig. S4. Mitochondrial translation initiation requires MTIF3 for assembly of OXPHOS complexes (related to Figs. 3 and 5).
• Fig. S5. Changes in the mitochondrial proteome of 10-week-old Mtif3 knockout mice (related to Fig. 4).
• Fig. S6. The effects of MTIF3 loss on mRNA recognition (related to Fig. 5).
• Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Micorsoft Excel format). Mitochondrial proteome of Mtif3 knockout mice compared to control mice.