LOCUS BXB1kan_PROMOTER_3079GALPppopt 4019 bp ds-DNA circular 06-MAY-2019 DEFINITION . ACCESSION urn.local...7wy-afoiakz KEYWORDS . SOURCE ORGANISM . . COMMENT COMMENT COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers rep_origin 35..717 /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ColE1 origin" rep_origin 95..714 /locus_tag="/product="" /label=pBR322_replication_origin Replication origin|rep_or igin /ApEinfo_fwdcolor=""#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator complement(906..954) /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="L3S3P22 terminator" terminator 967..1008 /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ECK120035133" gene complement(1018..1812) /gene="nptII" /vntifkey=60 /ApEinfo_fwdcolor="#66ff66" /ApEinfo_revcolor="#66ff66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="nptII" CDS complement(1018..1812) /gene="nptII" /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="ACJ66844.1" /db_xref="GI:215434895" /vntifkey=4 /ApEinfo_fwdcolor="#66ff66" /ApEinfo_revcolor="#66ff66" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="neomycin\phosphotransferase" primer_bind 1519..1535 /ApEinfo_fwdcolor="#76788c" /ApEinfo_revcolor="#76788c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="(oJE529)_pJE990_seq_2" -10_signal complement(1977..1985) /ApEinfo_fwdcolor="#07e500" /ApEinfo_revcolor="#07e500" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="-10 KanR (BPROM_pred)" -35_signal complement(2003..2008) /ApEinfo_fwdcolor="#07e500" /ApEinfo_revcolor="#07e500" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="-35 KanR (BPROM_pred)" regulatory 2132..2163 /regulatory_class="terminator" /note="TonB terminator" /ApEinfo_fwdcolor="#ff4649" /ApEinfo_revcolor="#ff4649" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="TonB terminator" source complement(<2178..>2341) /organism="Pseudomonas putida KT2440" /mol_type="genomic DNA" /strain="KT2440" /db_xref="taxon:160488" gene <2178..2262 /gene="aroF-II" /locus_tag="PP_3080" /db_xref="GeneID:1043781" CDS <2178..2262 /gene="aroF-II" /locus_tag="PP_3080" /EC_number="2.5.1.54" /note="Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 2903857, 6104668, 6146618, 9387, 6782075, 6396419, 2857723, 3294794, 3041242, 2903857, 2656411, 1970560, 1977738, 5335890, 11506923, 12402320, 16828913, 22127754, 9056491; Product type e : enzyme; Aminoacidbiosynthesis : Aromatic amino acid family" /transl_table=11 /product="tyr-sensitive phospho-2-dehydro-3-deoxyheptonatealdolase" /protein_id="NP_745224.1" /db_xref="GI:26989799" /db_xref="GeneID:1043781" /translation="MHASSLALPLTQPQAANTTVSRRLPSPHLLKQQMPLPSELAQQV QAHRQAIRNILEGRDPRLLVIVGPCSIHDPRSALEYADRLAALGREVNDKLLLVMRAY VEKPRTTVGWKGLAYDPHLDGSDDMHSGIALSRGLMLSMIERGLPIATELLQPMAAGY FDDLLAWAAIGARTTESQIHREMVSGLELPVGFKNGTDGGIAIASDAMRSAAHPHRHF GMDAQGHPAIIETLGNPDTHLVLRGGHKGPNHDANSIAMARQALAKAGLQARIMVDCS HANSGKDPARQPAVFNDVLEQRLAGDRSLVGVMIEGHLFDGCQALGKGTLKYGVSITD GCLGWASTETLLREAAQKL" /codon_start=2 misc_feature 2280..2285 /created_by="gp0" /label="-35 region" misc_feature 2305..2313 /created_by="gp0" /modified_by="gp0" /label="-10 region" CDS 2345..3739 /ApEinfo_fwdcolor="#acdcff" /ApEinfo_revcolor="#acdcff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="Ec_galP (Ppu opt)" misc_feature 3740 /ApEinfo_fwdcolor="#b90746" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="mKate2 optimized" terminator complement(3747..3797) /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="L3S1P13 Terminator" terminator complement(3805..3849) /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ECK120010850" misc_recomb 3858..3915 /locus_tag="/product="" /label=BxB1 attP|misc_recomb /ApEinfo_fwdcolor=""#06ff00" /ApEinfo_revcolor="#06ff00" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /modified_by="gp0" /label="attP bxb1" terminator 3922..3974 /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ECK120033736" ORIGIN 1 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 61 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 781 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 901 cctctgggag accagaaaca aaaaaaggcc gcgttagcgg ccttcaataa ttggacctgg 961 ctcctaactg atttttaagg cgactgatga gtcgcctttt ttttgtctaa gaattcatca 1021 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc 1081 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt 1141 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc 1201 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac 1261 gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag 1321 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg 1381 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt 1441 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga 1501 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt 1561 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc 1621 tgcctcgtct tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg 1681 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc 1741 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc 1801 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg 1861 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt 1921 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc 1981 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc 2041 ccttgtccag atagcccagt agctgacatt catccgggac gtcgtgcccc aactggggta 2101 acctttgagt tctctcagtt gggggatcga tagtcaaaag cctccggtcg gaggcttttg 2161 actagcacct cggtaccgaa atacggtgtg tcgattaccg acggctgcct gggctgggcc 2221 tcgaccgaaa ccctgttgcg tgaggcggca caaaaactgt aggccaatgg ttgcaagcct 2281 ggcgagacat accccggaca agtgcgctag gcttgccttt ttacccccaa ggagcagtac 2341 ccatatgcct gacgccaaga aacaaggtcg ctcgaacaaa gcaatgacct tcttcgtgtg 2401 cttcctggcc gccctggccg gtctgctgtt cggcctggac attggcgtga ttgcgggcgc 2461 cctcccgttc attgccgacg agtttcagat cacctcgcac acccaggagt gggtcgtgag 2521 cagcatgatg ttcggcgcgg ccgtgggggc cgtcggcagc ggttggctga gcttcaagct 2581 gggccgcaag aagagcctca tgatcggtgc aatcctgttc gtcgccggga gcttgttctc 2641 ggccgcggcc ccgaacgtcg aagtgctgat cctgagccgc gtcctgctgg gcctcgccgt 2701 gggcgtggcc tcgtacaccg cgcccctgta cttgtcggag atcgccccgg aaaaaatccg 2761 cggctcgatg atctccatgt accaactgat gatcaccatt ggcatcctgg gcgcctatct 2821 gagcgacacc gcgttctcgt acaccggcgc gtggcgttgg atgctgggcg tcatcatcat 2881 cccagcgatc ctgctgctga ttggcgtgtt cttcctgccg gattcgccgc gctggtttgc 2941 cgccaagcgc cgcttcgtgg acgccgagcg ggtgctcctg cgcctgcgtg acaccagcgc 3001 ggaagccaag cgggaactgg atgaaattcg cgagtccctg caggtgaagc agagcgggtg 3061 ggcgctgttc aaagagaact cgaatttccg ccgcgccgtg ttcctgggcg tcctcctgca 3121 ggtgatgcaa cagttcaccg gcatgaacgt gatcatgtac tatgccccca agatcttcga 3181 gctggccggt tacacgaaca ccaccgagca gatgtggggt acggtcattg tgggcctgac 3241 caacgtgctc gccaccttca tcgccattgg cctggtggac cgctgggggc gtaagccgac 3301 cctgaccctg ggtttcctgg tcatggccgc cggcatgggc gtgctgggca ccatgatgca 3361 catcggcatc cactccccct ccgcccaata cttcgccatc gccatgttgc tcatgttcat 3421 cgtcggcttc gccatgagcg caggtccgct gatctgggtg ctgtgtagcg agattcagcc 3481 gctcaagggt cgcgatttcg gtattacctg tagcaccgcc acgaactgga tcgccaacat 3541 gattgtcggc gcgaccttcc tcaccatgct gaataccttg ggcaacgcca ataccttctg 3601 ggtgtacgcc gccctcaacg tcctgtttat cttgctgacc ctgtggctcg tcccggaaac 3661 caagcatgtg agcctcgagc atattgagcg caatctgatg aagggccgca aactgcgcga 3721 aatcggcgcc catgattaaa tctagagacg aacaataagg cctccctaac ggggggcctt 3781 ttttattgat aacaaaaatc cacaaggaaa aattaaaggg gagataaaat cccccctttt 3841 tggttaactg cggccgcgtc gtggtttgtc tggtcaacca ccgcggtctc agtggtgtac 3901 ggtacaaacc ccgacgctag caacgcatga gaaagccccc ggaagatcac cttccggggg 3961 cttttttatt gcgctgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcc //