This PDF file includes:

• Fig. S1. Basic EXPAR system with hot start.
• Fig. S2. Detailed chemical reaction network of the molecular program for the detection of microRNAs.
• Fig. S3. Bistable amplification switch.
• Fig. S4. Experimental condition optimizations.
• Fig. S5. Extended data from Fig. 2A.
• Fig. S6. Droplet analysis.
• Fig. S7. Extended data from Fig. 3D.
• Fig. S8. Specificity of the trigger production.
• Fig. S9. RT-qPCR calibration.
• Fig. S10. Detection of cel-miR-39 in plasma sample.
• Fig. S11. Microfluidic chip design.
• Table S1. Oligonucleotide sequences used throughout the study.

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