This PDF file includes:

• Fig. S1. Conditions during the conjugation period effectively decouple conjugation from growth dynamics.
• Fig. S2. The log-linear correlation between T₀ and τ is maintained with respect to strain and choice of OD threshold.
• Fig. S3. Antibiotic resistance of plasmid donors.
• Fig. S4. Antibiotic modulation of conjugation for strain GN02766.
• Fig. S5. Identification of plasmid features lost in strain GN02766.
• Fig. S6. Sequencing read coverage plot for p2766-1.
• Fig. S7. Macrolide promotion of conjugation is transferrable.
• Table S1. All strains used in this study.
• Table S2. Plasmid composition of pathogenic E. coli isolates.
• Table S3. MICs and IC₅₀s for plasmid donors in this study.
• Model development and assumptions
• Reference (49)

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