This PDF file includes:

• Fig. S1. Deletion of either RAD51 or RAD52 impairs DNA damage repair process.
• Fig. S2. Deletion of either CLB2 or CLB3 affects the phosphorylation of Rad51 and Rad52 in cells.
• Fig. S3. CDK1 phosphorylates S125 and S375 of Rad51 and T412 of Rad52 in cells.
• Fig. S4. Nonphosphorylatable mutation of Rad51 impairs the strand invasion process even in the G₂/M phase–arrested cells.
• Fig. S5. CDK1-dependent phosphorylation regulates the DNA binding affinity of Rad51.
• Fig. S6. Nonphosphorylatable mutation of Rad52 impairs ligation process even in the G₂/M phase–arrested cells.
• Fig. S7. Both wild-type Rad52 and Rad52-T412A form ring structures.
• Fig. S8. Rapamycin treatment does not restore the defect in the ligation process in cells that express Rad52-T412A protein without FKBP or FRB attachment.
• Fig. S9. Full images of Western blots.
• Table S1. Yeast strains used in this study.

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