This PDF file includes:

• Table S1. DNA binding and self-association equilibrium constants of PU.1 constructs.
• Table S2. Primers in RT-PCR experiments.
• Fig. S1. Calibration of transgenic PU.1 dosage.
• Fig. S2. Characterization of a peptide-based PU.1 inhibitor.
• Fig. S3. ¹H-¹⁵N HSQC-detected titration of ΔN117 and ΔN165 by cognate DNA.
• Fig. S4. Purity of recombinant PU.1 constructs.
• Fig. S5. Spectral analysis of far-UV CD of ΔN165 in 0.15 and 0.05 M NaCl.
• Fig. S6. Salt-dependent line broadening of methyl protons in ΔN165.
• Fig. S7 The short C-terminal IDR is required for DNA-free PU.1 dimerization.
• Fig. S8. Effect of macromolecular crowding on dimerization of ΔN165 in the unbound and DNA-bound states.

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