,,,,,,,,,,,,,
Figure4 The expression of hepcidin and upstream regulators of hepcidin in thalassemic mice,,,,,,,,,,,,,
,,,delta Ct,,,,,,,,,,
Hepcidin mRNA expression in the liver,Average mActb Cq,Averag mHamp Cq,Cq mHamp - Cq mActb,,,,,,,,,,
WT 6-7 weeks old,23.07,19.69,-3.38,,,,,,,,,,
,22.90,19.03,-3.86,,,,,,,,,,
,22.67,19.16,-3.51,,,,,,,,,,
,22.76,21.11,-1.65,,,,,,,,,,
,23.32,19.51,-3.81,,,,,,,,,,
BKO 6-7 weeks old,23.11,20.36,-2.75,,,,,,,,,,
,22.49,19.04,-3.45,,,,,,,,,,
,22.68,21.05,-1.63,,,,,,,,,,
,22.68,18.83,-3.85,,,,,,,,,,
,22.81,18.58,-4.23,,,,,,,,,,
WT 16-20 weeks old,22.83,19.79,-3.04,,,,,,,,,,
,22.63,20.56,-2.06,,,,,,,,,,
,22.20,17.75,-4.45,,,,,,,,,,
,23.10,19.37,-3.72,,,,,,,,,,
,22.77,18.75,-4.02,,,,,,,,,,
BKO 16-20 weeks old,22.40,15.41,-6.99,,,,,,,,,,
,22.15,17.08,-5.06,,,,,,,,,,
,22.88,17.40,-5.48,,,,,,,,,,
,23.85,18.31,-5.53,,,,,,,,,,
,23.11,17.51,-5.60,,,,,,,,,,
,,,,,,,,,,,,,
,,,,,,,,,,,,,
Hepcidin/liver iron content,delta Ct hepcidin,liver iron content (nmole/mg tissue),Delta Ct hepcidin/liver iron,,,,,,,,,,
WT 6-7 weeks old,-3.38,1.63,-2.07,,,,,,,,,,
,-3.86,1.81,-2.13,,,,,,,,,,
,-3.51,3.37,-1.04,,,,,,,,,,
,-1.65,2.85,-0.58,,,,,,,,,,
,-3.81,1.83,-2.09,,,,,,,,,,
BKO 6-7 weeks old,-2.75,11.98,-0.23,,,,,,,,,,
,-3.45,12.84,-0.27,,,,,,,,,,
,-1.63,5.00,-0.33,,,,,,,,,,
,-3.85,5.19,-0.74,,,,,,,,,,
,-4.23,10.22,-0.41,,,,,,,,,,
WT 16-20 weeks old,-3.04,2.45,-1.24,,,,,,,,,,
,-2.06,2.24,-0.92,,,,,,,,,,
,-4.45,2.26,-1.97,,,,,,,,,,
,-3.72,1.73,-2.15,,,,,,,,,,
,-4.02,0.94,-4.26,,,,,,,,,,
BKO 16-20 weeks old,-6.99,10.41,-0.67,,,,,,,,,,
,-5.06,5.77,-0.88,,,,,,,,,,
,-5.48,10.85,-0.51,,,,,,,,,,
,-5.53,8.61,-0.64,,,,,,,,,,
,-5.60,7.98,-0.70,,,,,,,,,,
,,,,,,,,,,,,,
,,,delta Ct,,,,,,,,,,
Bmp6 mRNA expression in the liver,Average mActb Cq,Averag mBmp6 Cq,Cq mBmp6 - Cq mActb,,,,,,,,,,
WT 6-7 weeks old,23.07,30.59,7.52,,,,,,,,,,
,22.90,30.67,7.78,,,,,,,,,,
,22.67,30.71,8.04,,,,,,,,,,
,22.76,31.19,8.43,,,,,,,,,,
,23.32,30.96,7.64,,,,,,,,,,
BKO 6-7 weeks old,23.11,29.95,6.84,,,,,,,,,,
,22.49,28.58,6.09,,,,,,,,,,
,22.68,29.21,6.53,,,,,,,,,,
,22.68,29.61,6.93,,,,,,,,,,
,22.81,29.38,6.57,,,,,,,,,,
WT 16-20 weeks old,22.90,30.05,7.15,,,,,,,,,,
,22.82,30.33,7.51,,,,,,,,,,
,22.80,30.17,7.37,,,,,,,,,,
,22.83,30.57,7.74,,,,,,,,,,
,22.56,31.02,8.47,,,,,,,,,,
BKO 16-20 weeks old,22.93,29.84,6.91,,,,,,,,,,
,22.72,29.36,6.64,,,,,,,,,,
,22.91,28.92,6.01,,,,,,,,,,
,23.22,29.20,5.97,,,,,,,,,,
,22.89,28.69,5.80,,,,,,,,,,
,,,,,,,,,,,,,
,,,,,,,,,,,,,
,,,delta Ct,,,,,,,,,,
Gdf15 mRNA expression in the spleen,Average mActb Cq,Averag mGdf15 Cq,Cq mGdf15 - Cq mActb,,,,,,,,,,
WT 6-7 weeks old,20.39,33.94,13.54,,,,,,,,,,
,19.93,31.99,12.06,,,,,,,,,,
,20.24,32.70,12.46,,,,,,,,,,
,19.10,30.78,11.68,,,,,,,,,,
,19.64,31.69,12.05,,,,,,,,,,
BKO 6-7 weeks old,20.61,30.94,10.33,,,,,,,,,,
,20.63,29.22,8.59,,,,,,,,,,
,21.25,30.56,9.31,,,,,,,,,,
,21.30,33.06,11.75,,,,,,,,,,
,20.92,31.57,10.65,,,,,,,,,,
WT 16-20 weeks old,22.05,33.61,11.56,,,,,,,,,,
,21.71,33.03,11.31,,,,,,,,,,
,25.12,37.69,12.56,,,,,,,,,,
,20.43,33.08,12.65,,,,,,,,,,
,20.03,32.67,12.64,,,,,,,,,,
BKO 16-20 weeks old,19.87,30.61,10.75,,,,,,,,,,
,20.41,31.87,11.46,,,,,,,,,,
,20.48,31.09,10.61,,,,,,,,,,
,20.51,30.98,10.47,,,,,,,,,,
,20.93,31.14,10.20,,,,,,,,,,
,,,,,,,,,,,,,
,,,delta Ct,,,,,,,,,,
Twsg1 mRNA expression in the spleen,Average mActb Cq,Averag mTwsg1 Cq,Cq mTwsg1 - Cq mActb,,,,,,,,,,
WT 6-7 weeks old,20.39,34.04,13.65,,,,,,,,,,
,19.93,33.25,13.32,,,,,,,,,,
,20.24,34.26,14.02,,,,,,,,,,
,19.10,32.44,13.34,,,,,,,,,,
,19.64,32.38,12.75,,,,,,,,,,
BKO 6-7 weeks old,20.61,32.30,11.69,,,,,,,,,,
,20.63,31.46,10.83,,,,,,,,,,
,21.25,32.35,11.10,,,,,,,,,,
,21.30,33.25,11.94,,,,,,,,,,
,20.92,32.21,11.29,,,,,,,,,,
WT 16-20 weeks old,22.05,34.76,12.70,,,,,,,,,,
,21.71,34.51,12.79,,,,,,,,,,
,25.12,37.30,12.18,,,,,,,,,,
,20.43,32.75,12.33,,,,,,,,,,
,20.03,34.40,14.37,,,,,,,,,,
BKO 16-20 weeks old,19.87,31.07,11.21,,,,,,,,,,
,20.41,31.38,10.98,,,,,,,,,,
,20.48,31.34,10.86,,,,,,,,,,
,20.51,31.88,11.37,,,,,,,,,,
,20.93,32.10,11.16,,,,,,,,,,
,,,,,,,,,,,,,
,,,delta Ct,,,,,,,,,,
Erfe mRNA expression in the spleen,Average mActb Cq,Averag mFam132b Cq,Cq mFam132b - Cq mActb,,,,,,,,,,
WT 6-7 weeks old,20.39,34.41,14.02,,,,,,,,,,
,19.93,33.59,13.66,,,,,,,,,,
,20.24,34.80,14.56,,,,,,,,,,
,19.10,33.44,14.34,,,,,,,,,,
,19.64,31.58,11.95,,,,,,,,,,
BKO 6-7 weeks old,20.61,29.35,8.74,,,,,,,,,,
,20.63,28.34,7.71,,,,,,,,,,
,21.25,29.50,8.25,,,,,,,,,,
,21.30,30.88,9.57,,,,,,,,,,
,20.92,29.36,8.44,,,,,,,,,,
WT 16-20 weeks old,22.05,35.24,13.19,,,,,,,,,,
,21.71,35.06,13.35,,,,,,,,,,
,20.43,34.28,13.86,,,,,,,,,,
,20.03,34.06,14.03,,,,,,,,,,
BKO 16-20 weeks old,19.87,30.59,10.72,,,,,,,,,,
,20.41,30.02,9.62,,,,,,,,,,
,20.48,30.08,9.60,,,,,,,,,,
,20.51,30.75,10.24,,,,,,,,,,
,20.93,30.23,9.30,,,,,,,,,,
,,,,,,,,,,,,,