FungiEntomophthoralesAncylistaceaeNieYongYuDe-ShuiWangCheng-FangLiuXiao-YongHuangBoA taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new generaMycoKeys303202066558110.3897/mycokeys.66.46575C501E77E-6761-514E-8E81-5DC66636ED0F Conidiobolus coronatus 283037 (Costantin) A. Batko, Entomophaga, Mémoires hors série 2: 129 (1964)Figure 4 Boudierella coronataCostantin, Bull. Soc. mycol. Fr. 13: 40 (1897). Basionym. Delacroixia coronata(Costantin) Sacc. & P. Syd., Syll. fung. (Abellini) 14(1): 457 (1899). Entomophthora coronata(Costantin) Kevorkian, J. Agric. Univ. Puerto Rico 21(2): 191 (1937). = Conidiobolus villosus G.W. Martin, Bot. Gaz. 80(3): 317 (1925). Specimens examined.

China, Shandong Province, Plant detritus, 20 Mar 2009, C.F. Wang, RCEF 4518.

Description.

Colonies grown on PDA for 3 d at 21 °C, reaching ca. 65 mm in diameter. Mycelia colourless, 8–20 μm wide. Primary conidiophores, positively phototropic, colourless, unbranched and producing a single globose conidium, extending to a length of 53–287 μm into the air, 7.5–20.5 μm wide. Primary conidia forcibly discharged, colourless, globose, measuring 36–52 μm in greatest width and 42–65 μm in total length, including a basal papilla 12–18 μm high and 6.5–14 μm wide. After discharging on to 2% water-agar, similar and smaller secondary conidia arise from primary conidia. Microconidia produced readily from primary conidia, globose or almond-shaped, 13–19 × 11–15 μm. Villose spores formed after 4–5 d, globose, 20–42 μm.

Notes.

The ex-type living culture is ATCC 28691 (United States, Louisiana, Plant detritus, 3 January 1972). Due to the absence of molecular data of ex-type strain ATCC 28691, the molecular data of the authentic strain NRRL 28638, which has been applied in many other phylogenetic analysis (James et al. 2006; Liu and Voigt 2011; Gryganskyi et al. 2012; Tretter et al. 2014; Spatafora et al. 2016), was used in this study instead. The monotypic genus Delacroixia was typified by D. coronata which was transferred from an ascomycete Boudierella coronata Costantin (Costantin 1897; Saccardo and Sydow 1899). After that, it was reclassified as a subgenus of Conidiobolus, namely Conidiobolus sub. Delacroixia (Sacc. & P. Syd.) Tyrrell & MacLeod to define all those Conidiobolus species capable of forming microspores and, consequently, D. coronata was recombined as C. coronatus (Tyrrell and MacLeod 1972; Ben-Ze’ev and Kenneth 1982).

10.3897/mycokeys.66.46575.figure40DE74037-068A-518D-A599-C9BE5A317E2C

Conidiobolus coronatusa colony on PDA after 3 d at 21 °C b primary conidia c production of secondary conidia d, e primary conidiophores bearing primary conidia f, g microconidia h villose spores. Scale bars: 10 mm (a); 20 μm (b–h).

https://binary.pensoft.net/fig/395595
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