Sequence(4nt + 20nt protospacer  + PAM + 3nt)	Efficiency Score
AACTGAAGGCTGAACAGCAGGGGTGGGAGA	50.34572853
GGGGGAAAGAGGAAATGTGGACGAAGGCAG	49.79952186
ATCTGTCCCCTCCACCCCACAGTGGGGCCA	45.31911754
GGCAGCAACACAGCAGAGAGCAAGGGGAAG	37.19863205
GGGAGCTGGCTCAGGTTCAGGAGAGGGCAG	33.63310257
GGGGGAAAGAGGAAATGTGGACGAAGGCAG	29.1890051
AAGTGTAAGGAAGCTGCAGCACCAGGATCA	27.88861171
GCTAGCTCTTCCAGCCCCCTGTCATGGCAT	27.88751706
AAGTGTAAGGAAGCTGCAGCACCAGGATCA	26.9798325
ACATGCAGGGATCCAAGCACACAAGGTCCT	26.67260624
AAGTGCCTGCCTGGTCCGAATGCAGGGAGT	26.13372571
GGCAGCAACACAGCAGAGAGCAAGGGGAAG	25.95852768
GCCAGTCCCTGGTCCCCACACCCTGGGCGT	25.1379674
GGGAGCTGGCTCAGGTTCAGGAGAGGGCAG	22.87243816
GATGGAGAAAGAGAAAGGGAGTAGAGGCGG	22.55085866
ATCTGTCCCCTCCACCCCACAGTGGGGCCA	21.75532704
ATTAGTACTCATACCAAAAAAGAGTGAATA	19.89842003
AACTGAAGGCTGAACAGCAGGGGTGGGAGA	19.8604913
GATGGAGAAAGAGAAAGGGAGTAGAGGCGG	19.50601877
GCTAGCTCTTCCAGCCCCCTGTCATGGCAT	19.17376386
TCGTGTACAGTTAGTACTCAGCAGTGGACT	18.99598281
GACAGCATCTCTGTGTATACCAAACGATGC	18.63611072
CACAGGCTGCTGAGAAACCTGGCGGGACGC	18.26685575
CTCGGCCACCACAGGGAAGCTGGGTGAATG	17.76088459
CTCTGAGCGGATCCTCCCCGTGTCTGGGTC	17.4675292
ACACGGACACCCCCCTCCTCACCACAGCCC	17.30717021
GGGTGGCTACTGGCCTTATCTCACAGGTAA	15.48692816
TGCAGCTTCCTTACACTTCCCAAGAGGAGA	15.28576791
GGGGGCGCTCGGCCACCACAGGGAAGCTGG	14.90084273
CTCAGTACCTATCTGAGCATACCGTGTGTT	14.68922059
GCCGGTTAATGTGGCTCTGGTTCTGGGTAC	14.32425631
AACGGCTCTCAGGCCCTGTCCGCACGTAAC	14.26528832
CTCAGAAGACTTGGAGAAGCCAGAGGCTGT	13.22328718
TGCAGAAACAGCTGATTCTAGGGTTGGGAC	13.10034765
CCAGGGATCCTGTGTCCCCGAGCTGGGACC	12.95281984
GCCGGCGCCTGTGGCTTCTCTCAATGGAGG	12.52231243
TCTGGGCTTCTGGGTTTGAGTCCTTGCAAG	12.26441154
ATTGGCATTGCTGTCATCCTCGTGGGCACT	12.08198203
TACAGTTGCCCTCATTATTCAGCACGGCTG	11.80788591
TCTGGCGGTCTCAAGCACTACCTACGTCAG	11.44536653
GCCGGCGCCTGTGGCTTCTCTCAATGGAGG	10.70742119
GGGAGTAGAGGCGGCCACGACCTGGTGAAC	10.44433095
ACCTGGTGAACACCTAGGACGCACCATTCT	10.2398524
GAGAGCAAGGGGAAGAGGGAGTGGAGGAAG	9.974165136
TCTGGGCTTCTGGGTTTGAGTCCTTGGCAA	9.806876838
TCATGTCTGACCGGAAGATCCAGGGGGTGG	9.637771081
TCATGGCATCTTCCAGGGGTCCGAGAGCTC	8.760410654
TGAAGAGCCAAAGTTAGAACTCAGGACCAA	8.481470759
TTTAGGTAACTCAGCAGTTGAAGGCGCGAT	8.396933589
CTTAGGACTGTTTGAAAGGTGCAGAGAAAT	8.046494188
GAGAGCAAGGGGAAGAGGGAGTGGAGGAAG	8.039514859
TCATGGCATCTTCCAGGGGTCCGAGAGCTC	7.995786165
AATGGGGGTGTGTCACCAGATAAGGAATCT	7.845433255
TGCAGAGCAAATACCAGAGATAAGAGAGTA	7.809178265
TGCAGCTTCCTTACACTTCCCAAGAGGAGA	7.520563498
GATTGCGTACTTCGAAAAGGTAGGCGACAC	7.455885205
CCGTGGTTGCCCCCAACAGCCCCAGGATAT	7.438650307
GTAAGCAAACCTTAGAGGTTCTGGCAAGGA	7.217090069
CATGGCATCTTCCAGGGGTCCGAGAGCTCA	6.946408192
GGCAGTTAGAACTTTATCTTTCAGAGGTGG	6.690287439
ATGAGTAGACCAGGAGAATAAAGGAGAAAG	6.572413793
TGAAGAGCCAAAGTTAGAACTCAGGACCAA	6.526756946
GGTCATACTGTGTTAGCCTCTTAAGTACGT	6.418351511
CTGTGCTTTACCCAGTTCTCTGGGAGCAGG	6.292900799
TCTGGCTCCATCGTAAGCAAACCTTAGAGG	5.941918227
CAGGGCTCTGTCAGAAGATCTCCAGGTCCC	5.578575926
AGTTGGCTAAGAGAGGTTTCCGAAGGGTTT	5.46231858
GCCGGTTAATGTGGCTCTGGTTCTGGGTAC	5.138225745
GGCCGGGAAAGAGTTGCTGCACCAGGTGGT	4.866180049
TTCAGTACGGTGTTGAATACCAGTAGTAAA	4.685740032
CCCAGCGCTGTCTCCTGTCTATCTGGGTCT	4.627981815
AGCGGGCATCCTCCTCGTTGGATGTGGAGG	4.338877581
CATGGCATCTTCCAGGGGTCCGAGAGCTCA	4.117939681
GGCTGGCTACTGGCCTTATCTCACAGGTAA	4.031634707
TTGTGTTCTGTCACCAACTGAAGTGGCTAA	3.612647271
CCAGGGATCCTGTGTCCCCGAGCTGGGACC	3.546016011
ACACGGACACCCCCCTCCTCACCACAGCCC	3.319853966
CCCCGTCCTGGCAGGGCTGTGGTGAGGAGG	3.27075144
GCAGGAGGTGGGGAAGGCCGAAGCGGAGCG	2.996474736
ACCTGGTGAACACCTAGGACGCACCATTCT	2.958579882
AATGGGGGTGTGTCACCAGATAAGGAATCT	2.885492554
AATGGGGGTGTGTCACCAGATAAGGAATCT	2.679119098
CCTAGGTGTTCACCAGGTCGTGGCCGCCTC	2.657342657
CCCCGTCCTGGCAGGGCTGTGGTGAGGAGG	2.654688122
GTGCGTCCTAGGTGTTCACCAGGTCGTGGC	2.649563754
GTGCGTCCTAGGTGTTCACCAGGTCGTGGC	2.626059838
ATCAGCAACCATTCCTTCAGCTCATGGGCA	2.471019696
AAAAGTCTCTCTCTTAATGACACGGGCATC	2.377920032
TACCTTTGTACTACACCTTGCCCAGTAGCT	1.975970193
AGCAGGAGGATTTCGGAATCTTCCAGGTGC	1.967948773
GGAAGCTGCAGCACCAGGATCAGTGAAACG	1.83630555
GGCAGGGCTGTGGTGAGGAGGGGGGTGTCC	1.774708591
AGCGGGCATCCTCCTCGTTGGATGTGGAGG	1.591646189
CAAGGCAGATTTCTTGAGTCCAGGAGTTGA	1.475728155
GCAGGGCTGTGGTGAGGAGGGGGGTGTCCG	1.451211723
GGCAGGGCTGTGGTGAGGAGGGGGGTGTCC	1.435956597
GGCAGGGCTGTGGTGAGGAGGGGGGTGTCC	1.424870466
ATCTGCCTAACAGGAGGTGGGGGTTAGACG	1.292915873
GCATGTTTGCTGCCTCCAGGGATCCTGTGT	1.284558457
ACCTGGTGAACACCTAGGACGCACCATTCT	1.258254043
CCAAAGATGAGCTGCGTCAGATAGACGTCC	1.008539645
TGCTGCTTCGAAGTGCTTGAAAATGGTTTA	0.96479441
CACGGACACCCCCCTCCTCACCACAGCCCT	0.864275486
TGCTGCAGCTTCCTTACACTTCCCAAGAGG	0.848827472
AAAGGGAGTAGAGGCGGCCACGACCTGGTG	0.751878456
TTATAAAGCATTTCAAAAGGGGAAGTCTCG	0.721396806
CCGTGTGGAAAACTCCCTTTGTGAGAATGG	0.641411013
GGAAGCTGCAGCACCAGGATCAGTGAAACG	0.618005827
GTAAGGAAGCTGCAGCACCAGGATCAGTGA	0.588655452
ATCTGCCTAACAGGAGGTGGGGGTTAGACC	0.532667988
GCAGGGCTGTGGTGAGGAGGGGGGTGTCCG	0.515835789
TAGAGGCGGCCACGACCTGGTGAACACCTA	0.515463918
GGAAAAGTGTAAACCGGCATGTCAGCGACG	0.479815907
CCCAGAAGCCCAGAGCAGGGCCTTAGGGAA	0.434821805
TAGAGGCGGCCACGACCTGGTGAACACCTA	0.429088663
GGGAGTAGAGGCGGCCACGACCTGGTGAAC	0.409766483
GTAAGGAAGCTGCAGCACCAGGATCAGTGA	0.404571169
GCCGGCGGTCTGCGGTGCACATGCTGCGCT	0.37037037
CCTAGGTGTTCACCAGGTCGTGGCCGCCTC	0.32403036
GTTAGCAGACCCAGATAGACAGGAGACAGC	0.232851626
ACAGGCTGCTGAGAAACCTGGCGGGACGCT	0.197361382
TGGCGCCCCCTGTTGGGGGAACTCTGGAAT	0.139406771
CCGTGTGGAAAACTCCCTTTGTGAGAATGG	0.133141459
CGCTGTCTCCTGTCTATCTGGGTCTGCTAA	0.125269698
CTGTGGTGAGGAGGGGGGTGTCCGTGTGGA	0.11949244
TAGAGGCGGCCACGACCTGGTGAACACCTA	0.089336314
TGCTGCAGCTTCCTTACACTTCCCAAGAGG	0.086301702
AAGCGGACCCTGCTCTGGGCGGAGGAATAT	0.079372906
CTCAGCTAGTCTTCTTCCTCCAACCCGGGC	0.076951494
AAGAGGAGAAGCAGTTTGGAAAAACAAAAT	0.074074747
AGCGGGACCCTGCTCTGGGCGGAGGAATAT	0.07357371
GTAAGCAAACCTTAGAGGTTCTGGCAAGGA	0.070131866
GGGTGTCCGTGTGGAAAACTCCCTTTGTGA	0.058471477
GGGAGTAGAGGCGGCCACGACCTGGTGAAC	0.056130539
AGCAGCCTGTGGGAGCGCTTGCCTCAGAAC	0.053670916
GTAAGCAAACCTTAGAGGTTCTGGCAAGGA	0.05092298
TCTGGCTCCATCGTAAGCAAACCTTAGAGG	0.050266001
GGGTGTCCGTGTGGAAAACTCCCTTTGTGA	0.048671333
TGGCGGCTAGTCACTAAAGTCAAAAGCCCG	0.015936648
TCTGGCTCCATCGTAAGCAAACCTTAGAGG	0.005422712
GCATGTTTGCTGCCTCCAGGGATCCTGTGT	0.004725658
CACGGACACCCCCCTCCTCACCACAGCCCT	0.002743232
GCATGTTTGCTGCCTCCAGGGATCCTGTGT	0
CTCAGGACCAACTTATTCTGATTTTGTTTT	0
ACTAGAAAGGTGAAGAGCCAAAGTTAGAAC	0
CTGTGGTGAGGAGGGGGGTGTCCGTGTGGA	0
GACTGGGGTCAACGTTTGCACTATGACCTT	0
GCCGGGTTCCAACTCTTCTTGGGGCCGACG	0
AAGAGGAGAAGCAGTTTGGAAAAACAAAAT	-0.023434156
CTCAGGACCAACTTATTCTGATTTTGTTTT	-0.031721096
ACTAGAAAGGTGAAGAGCCAAAGTTAGAAC	-0.177752422