CRISPRa sequence (Boettcher group) (4bp + 20bp protospacer + PAM + 3bp)	Efficiency	CRISPRa sequence (Simeonov group) (4bp + 20bp protospacer + PAM + 3bp)	Efficiency
CTAGGGTCTGCTCTTTCCGGGAATGGGTGG	7.43	TGGTGCCTGGCTGCCAGGCAGGTAAGGGCC	-2.856288935
CTGGCGTGCCGGGGAGCGAGAAAAGGGGGA	3.44	GAGGAACTGCTATGTGTCAGGCACAGGGTT	-0.622568773
GGAGGATTCGGAGCCAGACGGTCGCGGCGC	0.53	GGTACAGGAGGAAGAGGGAAAGTCAGGGAG	-0.381248167
TCTCACTGCCTCCACCATGTGCTTTGGCTT	2.55	GGTGGAGGGGACTGTGGGGTGGAGGGGACT	-1.328623594
GGCACTCCAGGCCCTCTGGACCACCGGGTG	0.93	CTGGCTTGCCCCTGAATTCCTTCCTGGGCA	-0.187852554
GCTCCCTAGCTCCCTTCTACGAGTCGGGGG	4.97	TATGAATCCATCTTCCTGACCCTTGGGACC	-0.99984023
TGGTCGTCAGAACAGGACAATAAGAGGCTT	5.35	TCATGTTGCTCACATGGGCTCCAGGGGCCA	-0.543698842
TCCCCTCCCTCCCGCGGCCCCATTGGGCCG	1.92	AAAGCATAAGCTATTTTCCAGGAGAGGCAG	-0.124367847
AATCAAGGAGGATTCGGAGCCAGACGGTCG	0.3	GGGGGCGCGTTTTCTCCCCTGGGAAGGTTT	-0.06577467
TCCCTAGCTCCCTTCTACGAGTCGGGGGCT	2.36	CAACCTCTTGTGTGGTAGGACTTCCGGCTA	-0.312481148
GAGAGGTCCTTCGTACCGCAGGAGAGGCAG	4.67	CTGCAGGAGGCCGGCTGCTCTAGCAGGTTG	-0.038086281
GGCCAAAAGATCAGGCCTGCAACAGGGAAG	2.94	ACAAAAATAACTTCCCTGCTCACATGGCAC	0.272430694
TCACATCCTGAGCGAACGGGCGATGGGAGG	0.93	AGGCCCAGTCAACGTGATGTGTTTTGGAAC	0.406212986
CGTACCTACACAGCTCTGAGTCAAAGGCTG	3.56	CGTTGTGACTCTGTCAAAAAATACAGGACT	-0.028844398
CGGTCGCGGCGCAAGCAAACAACTCGGCTC	1.08	CAAAGTCCTGACAAGATTCTGGTCAGGCAG	-4.241199866
CAGTGGTGCAGAGAGAGGTGCAAGGGGCGC	0.87	TTTTTGGCATCGCGCCGGAGGATGTGGGAT	-0.140231325
ATCCTGAGCGAACGGGCGATGGGAGGGAGT	1.54	CAAAAACGCCCAAAGGGGACTGTGGGGTGG	-1.734683272
CCCCTCCCGGGTTCCCAAAGCAGAGGGCGT	11.3	AGGTGGCAATTCCTGAATGTGGTATGGTGA	-0.457207113
TGGGTGGGGTGCGCACTCCTCAACAGGGCT	4.14	GGCTTCTGAAGGAGGTATCTATTTTGGTCC	-1.385430004
TGCCTCCACCATGTGCTTTGGCTTTGGCTA	4.48	TTTCAAGTTCAATTGCTGGAGGTGTGGGCT	-2.605889037
GCGTCCCATCCTCACTAGCAAGCCTGGCGT	1.67	CTCTTAACAGATGGCCTCCTTGGTGGGCCT	-0.123662797
CGCAGTGGTGCAGAGAGAGGTGCAAGGGGC	2.4	CCCTCTTCCCAGCCACAGAACCAGAGGGAG	-1.171173071
CAGAGGGGTGCAGCACTTCAGGGAAGGACA	4.79	GGGTCCCCGTGGGTCACAGGCAAGAGGTGG	-3.973079972
TGACTAGAGTCCGAGAAGCAAAATGGGAGG	3.28	ATGTATACACTGCTTTTACGTCTTAGGAAA	0.246385899
ACACAAAGTTGAGACCGGTGTCCAGGGAGG	4.02	GCTGTAACGTCCTCAGGAGTCAGCCGGGAC	-3.284066231
CGAAGGCTGCGCCGCTCTAACAGGTGGGAT	7.57	GTGGGTGCCCCCGGAATTCCGGGAAGGCTG	-0.124715215
CCCCCACCCCATTGAGAACGTCAGGGGAGC	6.02	GAGGTTCCCACTCTTCAGCCAGTGAGGCCA	0.008212895
GGCAGCGGGAGCCTGAGCAAGAGCAGGATG	5.58	CTCACTGCATGTACCTGGGGCTGAAGGAGC	0.015998298
CCCCCGACTCGTAGAAGGGAGCTAGGGAGC	3.12	GTGTGTGCCTCTGAGGCGTGAGGGGGGGCG	-0.121369058
TTCGGGACCTGTAGACAGTAGGAGAGGACA	16.63	CTTATAAAATGACAATGCACTTTCAGGAGC	0.014224317
TGAGTCAAAGGCTGTCAGTCATCTCGGCTC	4.15	CTGAAGTTCAAAATTAGTGGTGTTTGGTTT	0.000233924
GTGCGTCCTGAGGAGGTGGCACGGGGGAGG	4.16	CTAAAGATGCCCCCAAGCTGCTCTGGGGGT	-0.445232852
ACCAGACTGAGAGACGAGGTTAGGAGGAAA	3.56	ACTCCCTTCTTGGAACCATCTACCTGGGGA	-2.223032603
TTCCCCCAAAGTGGCGGGGGAAGTGGGGGG	8.46	TATTGCTGAGAGTACAGAAAGCAGCGGCTT	-0.491240393
TTTACATCCTAGAGCTAGAGTGCTCGGCTG	17.03	CCTCTGCACAGACAATTATGACACAGGCTA	0.085819928
AAAAACTAGTTAGACGCTCAGCTTCGGTCA	6.22	GCAGTACAGGCAGCTGATGGGTTGAGGGTA	-0.3634593
GACAGTAGGAGAGGACAGGTAAAGAGGCCC	23.81	ACCTTTAGCAGCAGTTTAGACAGGAGGAAA	-0.00155136
GCCCCTGGCGCTGGCGGGTGTGAGGGGAAG	9.85	GTCCGCCTGGGCTGTCACCCTTGTGGGTCC	-3.977764905
CCGCAGGGTTGGCAGCGAAGCGCGGGGCGT	9.19	ACTGCCACGCTCCCAGAAAGCAAAGGGTTA	-2.479514644
TAATGTTCGGGACCTGTAGACAGTAGGAGA	26.12	AGGGGAATCTCCCTCTCCTTTTATGGGCGT	-0.991899006
CATCTGTGGTGAGGCTGATTGGCTGGGCAG	204.58	GTCAAGAGAGCCTGAAATAGCATTAGGTTC	-2.233545552
GCGGGGCTGGTCCCGGGTCACAGAGGGAGC	23.74	TGAGGCGGTACCCACAGAGCCGCGTGGCAC	-0.339254394
AGGCGTCCGCAGTGGTGCAGAGAGAGGTGC	5.16	CAGGTGGTCCTTAAGCCATCCTGCAGGAGG	0.00130782
TAAGATTGGTATCACAGGAAACTTGGGTAG	6.49	CCTTTTATGGGCGTAGCTGAAGAAAGGATT	-5.844882129
ACGGCTCGCAGGAAGCGCTCGCTAGGGTCT	9.22	TGGTCCTTGGGGTCTGGGTTCTCCGGGTCC	-1.163676294
GGTAGCAGCTGAGTGAACAGGAGAGGGAAG	16.01	AGCAGCATTATAATAGCATGGATTTGGTCT	0.160123149
AGCCGCAGGGTTGGCAGCGAAGCGCGGGGC	9.02	GTGCTGCTCAATCTGCATCCACAGCGGTTT	-0.676379403
GCCTCAGGAGAAAGCGAGACTCCGGGGCGC	15.25	AGAACCCAGACCCCAAGGACCAGGTGGTCC	-0.090877366
GATAGCCAAAGCCAAAGCACATGGTGGAGG	9.37	AACATGGGGAAATATGGGTCATAAAGGAGA	0.380582713
CCGGGTTCCCAAAGCAGAGGGCGTGGGGGA	19.53	GGACCAGCCGGGGCAGTGAAGCGGAGGTCT	-0.358183286
TGCGAAACAGGTTGAATTTCCAGGAGGAAT	46.57	GGGGAAGTGACCGCTCACGTCTGCAGGGAT	-0.727290649
CCGGCCGGGATGCAGGAGCGATGGAGGCAG	26.5	GGAGACTGTGAGCGTCCTCAGTGCAGGGCT	-2.140136331
GGCGGTGGCTTTCTGTATCCTCGCGGGTCC	24.23	AACAGAGGAAATTCTGAGCACCTCCGGGCG	-0.554307317
GGGCGGTGGTACCAGTCCTGCTGGCGGCTC	72.96	CTTCATAAGCTGAGTCCTCCCCTCAGGGCT	-4.073582487
ATCCGGGCCGGGAGCAGTCATCTGTGGTGA	51.14	CTTTAAGTCTTAGACAAATTTCCAGGGAGC	-0.742552209
TCTTGTCCCGGAACATGAGGTAGGTGGTGG	41.37	CTTCCTTCTACCCCCAGAGCAGCTTGGGGG	-1.656689412
AGGGGCCACTGCGTTCAGCTCTGGCGGTCC	82.06	GCAAAACCCAGCACATCTCACATCAGGGAC	-0.420901127
ACGGCAGGGTGACCCTAGCCCCGAGGGAGG	209.74	GGCTAGTACAGGTTATAAGCCTTGGGGAAT	-2.31250735
GCAGAGGGGGGAGGTTGCTTCTGTCGGAGG	190.89	GCAGAGAGCCTGCTGTTTATGTACTGGATG	-0.646153785
CTGGCCCAGACCTCTGTATTGGAAAGGTCT	301.08	TATTTTAATCAGGTTGACATTCTGTGGTGA	-0.249219402
		AGTTGCTCTCTTTAAGTATTGGGCTGGCGT	-5.599429467
		TAATTTCTTGAGAAACCTGCTCATCGGGAC	-0.637119632
		CAGCTTTGAGTGGCAACCTTAATGTGGTCC	-0.962360384
		AAGAGTGGGTGATTCTGTGGGCAGAGGTGG	-0.746970815
		GTGTGGCATGATATTTGGGATATTTGGATG	-0.660239107
		GGGTGAGCAAGCACTTCTTGGCAGTGGTCT	-7.651194265