Ophiosphaerella was introduced by Spegazzini (1909) to accommodate O. graminicola Speg. as the type species. The species of this genus are characterized by papillate ascomata bearing fissitunicate, cylindrical asci frequently narrower near the base, with a short furcate pedicel and filamentous, pale brown, multi-septate ascospores without swollen cells or separating into part spores. Barr (1987) placed Ophiosphaerella in Phaeosphaeriaceae and this was confirmed by Zhang et al. (2009, 2012) and Hyde et al. (2013) based on molecular phylogeny. Most Ophiosphaerella species are often found as pathogens or saprobes worldwide on Poaceae and Cyperaceae (Câmara et al. 2000). Currently, twelve Ophiosphaerella species are listed in Index Fungorum (2020). In this study, we introduce Ophiosphaerella taiwanensis from Agave tequilana F.A.C. Weber (Asparagaceae) as a new species.