LOCUS Exported 4107 bp ds-DNA circular SYN 04-JUN-2020 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4107) AUTHORS rvieira TITLE Direct Submission JOURNAL Exported Thursday, Jun 4, 2020 from SnapGene 5.1.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..4107 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" promoter 96..200 /label=KanR promoter /note="color: #ffffff; direction: RIGHT" CDS 201..1016 /codon_start=1 /label=KanR /note="color: #ccffcc" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 1187..1775 /direction=RIGHT /label=ori /note="color: #ffff00" primer_bind 2114..2133 /label=pUC57 fwd1 /note="color: black; sequence: tccggctcgtatgttgtgtg" primer_bind 2161..2178 /label=M13 Fw /note="color: black; sequence: caggaaacagctatgacc" misc_feature 2223..2322 /label=attB /note="attB" /note="color: #999999" misc_feature 2333..2366 /label=FRT 14 /note="FRT14" /note="color: #ff9900; direction: RIGHT" misc_feature 2417..2450 /label=FRT /note="FRT" /note="color: #ffff99; direction: RIGHT" misc_feature 2549..2580 /label=SD /note="Predicted by www.fruitfly.org" /note="color: #00ccff" CDS complement(2597..3316) /codon_start=1 /product="GFP" /label=GFP /label=EGFP /note="color: #00ff00" /translation="GSVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKD DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGI KANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVL LEFVTAAGITLGMDELYK" misc_feature 3338..3376 /label=SA /note="predicted using www.fruitfly.org" /note="color: #00ccff" misc_feature complement(3456..3489) /label=FRT14 /note="FRT14 " /note="color: #ff9900; direction: LEFT" misc_feature complement(3540..3573) /label=FRT /note="FRT" /note="color: #ffff99; direction: LEFT" misc_feature 3582..3681 /label=attB /note="attB " /note="color: #999999" primer_bind complement(3713..3730) /label=M13 rv /note="color: black; sequence: tgtaaaacgacggccagt" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagccata ttcaacggga aacgtcttgc tctaggccgc 241 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 301 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc 361 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 421 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 481 catggttact caccactgcg atccctggga aaacagcatt ccaggtatta gaagaatatc 541 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 601 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 661 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 721 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg 781 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 841 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 901 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 961 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaactgt 1021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 1081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 1141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 1201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 1261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 1321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 1381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 1441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 1501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 1561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 1621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 1681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 1741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 1801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 1861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 1921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 1981 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 2041 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 2101 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 2161 caggaaacag ctatgaccat gctcgagcca agctcggcgc gccattggga tgaaccgagc 2221 tcgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc 2281 ccgggcgcgt actccacctc acccatctgg tccatcatga tggaagactt ctgaagttcc 2341 tattctatca gaagtatagg aacttcgcag aatggtagct ggattgtagc tgctattagc 2401 aatatgaaac ctcttagaag ttcctattct ctagaaagta taggaacttc gaattctggc 2461 gacttgtcgc atctttctct gagtgggtaa ggtcattgtg gggtcttaag ggctaaaggg 2521 tagtgatgat gaaggcattt gatgccattg ttcaataact tacctcgccg gaacctccgc 2581 ttccaccgct acctcccttg tacagctcat ccatgcccag ggtgatgccg gcggcggtca 2641 cgaactccag cagcaccatg tgatcgcgct tctcgttggg gtccttggac agcacgctct 2701 gggtgctcag gtagtggtta tcgggcagca gcactgggcc gtcgccgatg ggggtgttct 2761 gctggtagtg atcggccagc tgcacggagc catcctccac attgtggcgg atcttgaagt 2821 tggccttgat gccgttcttc tgcttatcgg cggtgatgta cacgttgtgg ctgttgaagt 2881 tgtactccag cttgtggccc aggatgttgc catcctcctt gaaatcgatg cccttcagct 2941 cgatgcggtt caccagggta tcgccctcga acttcacctc ggcgcgggtc ttgtaggtgc 3001 cgtcatcctt gaagctgatg gtgcgctcct gcacgtagcc ctcgggcatg gcgctcttga 3061 agaaatcgtg ctgcttcatg tgatcggggt agcggctgaa gcactgcacg ccgtaggtca 3121 gggtggtcac cagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca 3181 gggtcagctt gccgttggtg gcgtcgccct cgccctcgcc gcgcacgctg aacttgtggc 3241 cgttcacgtc gccatccagc tccaccagga tgggcaccac gccggtgaac agctcctcgc 3301 ccttggacac ggatccggat ccgccgctac ctccgcttcc accggaacct cccacctgcg 3361 gaagagagat aaatcggttg attaaaatta gttcaagaat aattagtata tttaaacatg 3421 ccggggatgg gacaagtcgc catgttggat cgactgaagt tcctatactt ctgatagaat 3481 aggaacttct tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagag 3541 aagttcctat actttctaga gaataggaac ttcccgtctt ccatcatgat ggaccagatg 3601 ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc cctggcaccc gcaccgcggc 3661 ttcgagaccg tgacctacat caagcttgtt ccatcccaat acgcgtcaat tcactggccg 3721 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 3781 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 3841 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc 3901 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 3961 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 4021 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 4081 tttcaccgtc atcaccgaaa cgcgcga //