NETosis occurs independently of neutrophil serine proteases

Supporting Information

• Supporting Information - Description of supporting movies
• Movie 1 - Time course of NET formation in PMA-treated neutrophils co-incubated with a Cy5-labeled NE probe (red), and SYTOX GreenTM to mark DNA.
• Movie 2 - Nuclear DNA is contained within the nucleus during pyroptosis induced by treatment of macrophages by LPS and ATP, and SYTOX GreenTM to mark DNA
• Movie 3a - Z-stack of fresh neutrophils stimulated with PMA to induce NETosis BODIPY-labeled NE probe (green) and propidium iodide (red) to visualize DNA
• Movie 3b - Z-stack of fresh neutrophils stimulated with PMA to induce NETosis BODIPY-labeled CatG probe (green) and propidium iodide (red) to visualize DNA
• Movie 3c - Z-stack of fresh neutrophils stimulated with PMA to induce NETosis BODIPY-labeled PR3 probe (green) and propidium iodide (red) to visualize DNA