FungiDiaporthalesDiaporthaceaeYangQinJiangNingTianCheng-MingNew species and records of Diaporthe from Jiangxi Province, ChinaMycoKeys141202177416410.3897/mycokeys.77.59999FF202454-C6E7-5DAF-AFA6-F783DE9AA27A Diaporthe multiguttulata F. Huang, K.D. Hyde & Hong Y. Li, in Huang et al., Fungal Biology 119(5): 343. 2015.Figure 6Description.

Conidiomata pycnidial, 692–750(–800) μm diam., solitary and with single necks erumpent through host bark. Tissue around neck is cylindrical. Locule circular, undivided, 450–565(–600) μm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells unbranched, straight or slightly curved, apical or base sometimes swelling, (8.5–)9–10.5(–11) × 1.5–2 μm. Alpha conidia hyaline, aseptate, ellipsoidal, biguttulate or with one large guttulate, rounded at one end, slightly apex at the other end, occasionally submedian constriction, (7.5–)8–9(–10.5) × 4–5(–5.5) μm. Beta conidia not observed.

10.3897/mycokeys.77.59999.figure6107C5622-DAE3-5C6D-9465-E648297048B8

Diaporthe multiguttulata on Citrus maxima (BJFC-S1614) a, b habit of conidiomata on twig c conidiomata on PDAd transverse section through conidiomata e longitudinal section through conidiomata f conidiogenous cells attached with alpha conidia g alpha conidia h the colony on PDA. Scale bars: 200 μm (b, d, e); 10 μm (f, g).

https://binary.pensoft.net/fig/497466Culture characters.

Colony originally flat with white felty aerial mycelium, becoming pale green mycelium with age, margin area irregularly, with visible solitary conidiomata at maturity.

Specimens examined.

China. Jiangxi Province: Ganzhou City, on branches of Citrus maxima, 25°51'28"N, 114°55'19"E, 11 May 2018, Q. Yang, Y. Liu & Y.M. Liang (BJFC-S1614; living culture: CFCC 53095, CFCC 53096 and CFCC 53097).

Notes.

Diaporthe multiguttulata was originally described as an endophyte from a healthy branch of Citrus grandis in Fujian Province, China (Huang et al. 2015). In the present study, three isolates (CFCC 53095, CFCC 53096 and CFCC 53097) from symptomatic branches of Citrus maxima were congruent with D. multigutullata, based on DNA sequence data and confirmed from the morphological analysis (Fig. 1). The clade, therefore, was verified as D. multigutullata which could exist both as an endophyte and a pathogen.

10.3897/mycokeys.77.59999.figure13BD327BB-DE45-5565-B923-CC5DDA9E7E26

Phylogram of Diaporthe from a Maximum Likelihood analysis based on combined ITS, cal, his3, tef1 and tub2. Values above the branches indicate Maximum Likelihood bootstrap (left, ML BP ≥ 50%) and Bayesian probabilities (right, BI PP ≥ 0.90). The tree is rooted with Diaporthella corylina. Strains in current study are in blue font and the ex-type cultures are in bold font.

https://binary.pensoft.net/fig/497460HuangFUdayangaDWangXHouXMeiXFuYHydeKDLiHY (2015) Endophytic Diaporthe associated with Citrus: A phylogenetic reassessment with seven new species from China.Fungal Biology119: 331–347. https://doi.org/10.1016/j.funbio.2015.02.006