# Epstein & Tiffin, Comparative genomics reveals high rates of horizontal transfer and strong purifying selection on rhizobial symbiosis genes # Supplementary Table S1 # List of all strains included in the initial steps of the analysis # Strains with a "1" in the "Target" column were included in HT and selection analyses # GTDB species is the species name given by Genome Taxonomy Database (https://gtdb.ecogenomic.org) # The assemblies for two of the strains were downloaded from MaGe (https://mage.genoscope.cns.fr/microscope/home/index.php) instead of NCBI Strain NCBI Species GTDB Species RefSeq Accession Genome Size Target Assembly Level Unique Genes ORS571 Azorhizobium caulinodans Azorhizobium caulinodans GCF_000010525.1 5369772 1 Complete Genome 756 USDA110 Bradyrhizobium diazoefficiens Bradyrhizobium diazoefficiens GCF_000011365.1 9105828 1 Complete Genome 711 LMTR13 Bradyrhizobium icense Bradyrhizobium icense GCF_001693385.1 8322773 1 Complete Genome 1265 USDA6 Bradyrhizobium japonicum Bradyrhizobium japonicum GCF_000284375.1 9207384 1 Complete Genome 926 ORS285 Bradyrhizobium sp. Bradyrhizobium sp. GCF_900176205.1 7797098 1 Complete Genome 952 CCNWGS0123 Mesorhizobium amorphae Mesorhizobium amorphae GCF_001686985.1 7343952 1 Complete Genome 613 WSM2073 Mesorhizobium australicum Mesorhizobium australicum GCF_000230995.2 6200534 1 Complete Genome 417 MAFF303099 Mesorhizobium loti Mesorhizobium japonicum GCF_000009625.1 7596297 1 Complete Genome 773 ORS2060 Methylobacterium nodulans Methylobacterium nodulans GCF_000022085.1 8839022 1 Complete Genome 2826 HAMBI540 Neorhizobium galegae Neorhizobium galegae GCF_000731315.1 6455027 1 Complete Genome 583 N561 Rhizobium esperanzae Rhizobium esperanzae GCF_001664265.1 6478651 1 Complete Genome 258 CFN42 Rhizobium etli Rhizobium etli GCF_000092045.1 6530228 1 Complete Genome 192 IE4803 Rhizobium etli Rhizobium sophorae GCF_000816125.1 6997434 1 Complete Genome 314 Mim1 Rhizobium etli Rhizobium etli GCF_000442435.1 7197998 1 Complete Genome 244 R602 Rhizobium gallicum Rhizobium azibense GCF_000816845.1 7311190 1 Complete Genome 569 3841 Rhizobium leguminosarum Rhizobium leguminosarum GCF_000009265.1 7751309 1 Complete Genome 401 WSM1325 Rhizobium leguminosarum Rhizobium leguminosarum GCF_000023185.1 7418122 1 Complete Genome 368 WSM2304 Rhizobium leguminosarum Rhizobium acidisoli GCF_000021345.1 6872702 1 Complete Genome 327 N161 Rhizobium phaseoli Rhizobium phaseoli GCF_001664165.1 6720332 1 Complete Genome 227 CCGE532 Rhizobium sp. Rhizobium sp. GCF_003627775.1 6923224 1 Complete Genome 327 IRBG74 Rhizobium sp. Agrobacterium pusense GCF_000499645.1 5464982 1 Complete Genome 495 NXC24 Rhizobium sp. Rhizobium sp. GCF_002944315.1 7302537 1 Complete Genome 523 CIAT899 Rhizobium tropici Rhizobium tropici GCF_000330885.1 6686334 1 Complete Genome 324 CCGM7 Sinorhizobium americanum Sinorhizobium americanum GCF_000705595.2 6853050 1 Complete Genome 368 NGR234 Sinorhizobium fredii Sinorhizobium fredii GCF_000018545.1 6891900 1 Complete Genome 351 WSM419 Sinorhizobium medicae Sinorhizobium medicae GCF_000017145.1 6817576 1 Complete Genome 384 1021 Sinorhizobium meliloti Sinorhizobium meliloti GCF_000006965.1 6691694 1 Complete Genome 393 UFLA1-100 Azorhizobium doebereinerae Azorhizobium doebereinerae GCF_000473085.1 5817514 0 Scaffold NA RST91 Bradyrhizobium algeriense Bradyrhizobium algeriense GCF_003062295.1 8907982 0 Scaffold NA USDA3384 Bradyrhizobium arachidis Bradyrhizobium arachidis GCF_000472685.1 9800269 0 Scaffold NA UBMA182 Bradyrhizobium canariense Bradyrhizobium canariense GCF_002108845.1 8216640 0 Contig NA PAC68 Bradyrhizobium jicamae Bradyrhizobium jicamae GCF_001440395.1 8738846 0 Scaffold NA CCNWSX0360 Bradyrhizobium liaoningense Bradyrhizobium liaoningense GCF_001595995.1 8590559 0 Contig NA BR10303 Bradyrhizobium macuxiense Bradyrhizobium macuxiense GCF_001542415.1 8722143 0 Scaffold NA BR3351 Bradyrhizobium manausense Bradyrhizobium manausense GCF_001440035.1 9145311 0 Scaffold NA ARR65 Bradyrhizobium sp. Bradyrhizobium sp. GCF_000472385.1 8613869 0 Contig NA Ai1a-2 Bradyrhizobium sp. Bradyrhizobium sp. GCF_000426245.1 9029266 0 Scaffold NA Cp5.3 Bradyrhizobium sp. Bradyrhizobium neotropicale GCF_000426265.1 8918781 0 Scaffold NA Tv2a-2 Bradyrhizobium sp. Bradyrhizobium sp. GCF_000472425.1 8496279 0 Contig NA th.b2 Bradyrhizobium sp. Bradyrhizobium sp. GCF_000426785.1 10118060 0 Scaffold NA BR446 Bradyrhizobium stylosanthis Bradyrhizobium stylosanthis GCF_001641335.1 8801717 0 Contig NA LmjM3 Bradyrhizobium valentinum Bradyrhizobium valentinum GCF_001440405.1 8845258 0 Scaffold NA BR3267 Bradyrhizobium yuanmingense Bradyrhizobium yuanmingense GCF_001439885.1 7904309 0 Scaffold NA STM6070 Cupriavidus taiwanensis Cupriavidus taiwanensis GCF_000372525.1 6771773 0 Contig NA ca181 Mesorhizobium ciceri Mesorhizobium wenxiniae GCF_000474725.1 7041479 0 Chromosome NA UFLA01-765 Mesorhizobium loti Mesorhizobium loti GCF_001510895.1 7464539 0 Contig NA ORS3324 Mesorhizobium sp. Mesorhizobium sp. GCF_000824825.2 6580560 0 Scaffold NA WSM3557 Microvirga lotononidis Microvirga lotononidis GCF_000262405.1 7082538 0 Scaffold NA DSM21826 Neorhizobium alkalisoli Neorhizobium alkalisoli GCF_002968635.1 5869725 0 Contig NA IPA7.2 Ochrobactrum cytisi Ochrobactrum cytisi GCF_001876955.1 5965364 0 Scaffold NA LUP21 Ochrobactrum lupini Ochrobactrum anthropi GCF_002252535.1 5582813 0 Contig NA TJ182 Paraburkholderia caribensis Paraburkholderia caribensis GCF_003028645.1 9205673 0 Contig NA LMG26031 Paraburkholderia diazotrophica Paraburkholderia diazotrophica GCF_900108945.1 8688577 0 Scaffold NA WSM3556 Paraburkholderia dilworthii Paraburkholderia dilworthii GCF_000472525.1 7679067 0 Scaffold NA KB15 Paraburkholderia kirstenboschensis Paraburkholderia kirstenboschensis GCF_001636975.1 8342886 0 Contig NA LMG23256 Paraburkholderia mimosarum Paraburkholderia mimosarum GCF_000472825.1 8410967 0 Scaffold NA DSM21604 Paraburkholderia nodosa Paraburkholderia nodosa GCF_000519185.1 9627966 0 Scaffold NA BR3459a Paraburkholderia phenoliruptrix Paraburkholderia phenoliruptrix GCF_000300095.1 7651131 0 Complete Genome NA STM815 Paraburkholderia phymatum Paraburkholderia phymatum GCF_000020045.1 8676562 0 Complete Genome NA WSM3937 Paraburkholderia rhynchosiae Paraburkholderia rhynchosiae GCF_002879865.1 8029899 0 Contig NA WSM5005 Paraburkholderia sprentiae Paraburkholderia sprentiae GCF_001865575.1 7829542 0 Complete Genome NA WSM4176 Paraburkholderia tuberum Paraburkholderia tuberum GCF_000372945.1 9065247 0 Scaffold NA CCBAU03422 Phyllobacterium sophorae Phyllobacterium sophorae GCF_003010965.1 6358399 0 Contig NA LPU83 Rhizobium favelukesii Rhizobium favelukesii GCF_000577275.1 7038809 0 Chromosome NA H152 Rhizobium giardinii Rhizobium giardinii GCF_000379605.1 6810126 0 Scaffold NA FH14 Rhizobium hidalgonense Rhizobium hidalgonense GCF_002531855.1 7268818 0 Contig NA 4292 Rhizobium leguminosarum Rhizobium leguminosarum GCF_000379005.1 7346596 0 Scaffold NA STM3625 Rhizobium mesoamericanum Rhizobium mesoamericanum GCF_000312665.1 6461927 0 Scaffold NA LCM4573 Rhizobium sp. Neorhizobium sp. GCF_001854865.1 5521535 0 Contig NA 14971 Rhizobium taibaishanense Allorhizobium taibaishanense GCF_001938985.1 5418020 0 Scaffold NA LMG14919 Sinorhizobium arboris Sinorhizobium arboris GCF_000427465.1 6850303 0 Scaffold NA LMG7837 Sinorhizobium saheli Sinorhizobium saheli GCF_001651875.1 5994348 0 Scaffold NA USDA4894 Sinorhizobium terangae Sinorhizobium terangae None (from MaGe) 7081278 0 Draft NA USDA1106 Sinorhizobium meliloti Sinorhizobium meliloti None (from MaGe) 6716226 Complete Genome NA