Protein Temperature (¼C) Start End Charge Sequence Time Point (min) replicate Average (daltons) SD Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 -4.83E-13 0.061823751 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 5.247616926 0.102639431 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 5.258652031 0.695667255 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 5.084212484 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 5.024792687 0.26410146 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 5.882559899 1.938144578 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 6.655866114 1.109826363 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 5.74080124 0.552812374 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 8.556026335 1.663839198 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 8.914242825 1.537790774 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 9.340367655 0.770696079 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 9.256331085 1.019191543 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 10.03600371 1.223870175 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 9.328908122 0.809711067 Leu343Ala 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.92178311 0.697467947 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 4.83E-13 0.046217756 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 6.252660348 1.093019906 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 6.562916574 1.115228438 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 7.126131364 0.608033589 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 6.515805164 0.312720138 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 7.045065784 0.056421676 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 9.615820856 0.159061107 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 9.197760142 0.915951882 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 10.24949055 0.989180014 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 10.44939572 0.125448194 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.48716974 0.506594619 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 10.82671143 0.420161414 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 11.40181018 0.983777939 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 11.7651198 0.186671714 Leu343Ala 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 11.33390184 0.542008224 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 0 0.087033436 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 6.978430726 0.07142744 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 5.919060631 0.177068024 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 7.463126498 0.149457417 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 6.676663042 0.098437817 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 6.641011164 0.545009377 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 9.23638301 0.668056648 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 8.852700892 0.399153343 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 9.210917383 0.486787009 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 10.39676676 0.209480476 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.86703201 0.015005765 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 10.24736841 0.414759338 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 11.59322681 1.222069483 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 10.74097715 1.27008793 Leu343Ala 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.69768559 0.367341122 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 -4.83E-13 0.086433205 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 6.19493826 0.50779508 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 6.41606479 0.325925211 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 8.460530233 0.471781244 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 7.159236679 0.519799692 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 8.073028271 1.187856339 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 9.088682372 0.744285933 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 9.873023692 0.925555571 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 10.13531965 0.362539277 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 10.8559969 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.32800957 0.199276556 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 10.75922752 0.235290392 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 10.6947146 1.264085625 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 11.03128531 0.407556571 Leu343Ala 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.62001542 0.440569254 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 4.83E-13 0.089434358 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 7.918112372 0.526402228 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 8.285241832 0.241292698 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 8.548386647 1.470564948 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 9.316175309 1.014989929 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 9.42355537 1.19806026 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 9.857744315 0.450773174 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 10.59327652 0.258099154 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 11.34875679 0.196875634 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 11.38440867 1.183654725 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 11.0249189 0.557614219 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 10.72951762 0.830118907 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 10.41034843 0.331927517 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 11.06736161 2.245462641 Leu343Ala 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 11.01473265 0.065425134 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 -4.83E-13 0.024609454 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 5.317222973 0.108641737 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 5.057049148 0.090034589 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 6.285765664 0.207079554 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 6.079494083 0.378745503 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 5.338019902 0.199276556 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 6.773007999 0.682462182 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 6.587957774 0.536005918 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 7.873971951 0.772496771 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 8.741500987 0.875736432 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 9.644681901 0.662054342 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 9.231289885 0.139853728 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 9.453265269 0.673458723 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 10.47740791 1.133835586 WT 10C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 9.885756505 0.045017294 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 0 0.135051883 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 5.475958717 0.939360875 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 6.502223496 0.022208532 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 6.226770294 1.25928378 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 6.225072586 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 5.697509674 0.18307033 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 8.008090921 1.375128284 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 8.064115301 1.995766715 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 9.317873017 0.321123366 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 9.599268199 0.255698232 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.4803789 1.761076554 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 11.77530605 0.197475864 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 9.750364254 0.747887316 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 9.718107792 0.205278862 WT 20C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.22190279 0.778499076 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 0 0.089434358 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 6.686849293 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 6.318870979 0.156660184 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 8.00681764 1.228071789 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 7.118916103 1.601415217 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 8.301370062 1.821099613 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 9.543243819 2.102007529 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 8.717733068 0.788102766 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 10.23336232 1.139237662 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 11.51173681 1.49337371 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.70956955 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 11.08943182 1.64523205 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 11.32498887 0.523401075 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 10.54956052 1.166248038 WT 25C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.83222899 1.175851728 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 -4.83E-13 0.05041937 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 6.722925599 0.82231591 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 6.361313691 0.160861798 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 7.117218394 0.189072636 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 7.422805921 0.795305533 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 7.918112372 0.249695926 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 8.531409562 0.099038048 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 9.739329149 0.708872328 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 9.74357342 0.004201614 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 10.57969485 1.024593619 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 9.954513699 0.085232744 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 10.11112731 0.574420676 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 120 1 10.00035183 0.254497771 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 10.1298021 0.479584242 WT 30C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 10.28259586 0.634443735 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0 1 0 0.069026518 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.166666667 1 7.506418064 0.419561183 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.333333333 1 8.19399 2.032981012 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.5 1 8.51697904 0.967571713 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 0.75 1 8.662133115 0.787502535 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 1 1 9.297924943 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 3 1 11.29782554 0.746686855 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 5 1 11.56394134 0.045017294 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 10 1 11.1547936 0.114644043 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 30 1 11.13824094 0.662654572 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 60 1 10.50669338 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 90 1 11.32838429 0.8535279 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 180 1 10.07420215 0.267102613 WT 40C 2 23 3 AVSKVYARSVYDSRGNPTVEVE 240 1 11.02619218 1.094820598 Leu343Ala 10C 22 31 2 VELTTEKGVF 0 1 1.96E-13 0.009022636 Leu343Ala 10C 22 31 2 VELTTEKGVF 0.166666667 1 2.112803634 0.089250937 Leu343Ala 10C 22 31 2 VELTTEKGVF 0.333333333 1 2.170913063 0.226053603 Leu343Ala 10C 22 31 2 VELTTEKGVF 0.5 1 2.445079208 0.285066518 Leu343Ala 10C 22 31 2 VELTTEKGVF 0.75 1 2.291787571 0.12875545 Leu343Ala 10C 22 31 2 VELTTEKGVF 1 1 2.538709533 0.304818774 Leu343Ala 10C 22 31 2 VELTTEKGVF 3 1 3.114803301 0.161188168 Leu343Ala 10C 22 31 2 VELTTEKGVF 5 1 3.118941658 0.113392584 Leu343Ala 10C 22 31 2 VELTTEKGVF 10 1 3.927818003 0.238002499 Leu343Ala 10C 22 31 2 VELTTEKGVF 30 1 4.523051672 0.304331064 Leu343Ala 10C 22 31 2 VELTTEKGVF 60 1 4.725486297 0.079496736 Leu343Ala 10C 22 31 2 VELTTEKGVF 90 1 4.83791166 0.057549785 Leu343Ala 10C 22 31 2 VELTTEKGVF 120 1 4.996203812 0.022434662 Leu343Ala 10C 22 31 2 VELTTEKGVF 180 1 5.127769075 0.091933342 Leu343Ala 10C 22 31 2 VELTTEKGVF 240 1 5.10811188 0.100712123 Leu343Ala 20C 22 31 2 VELTTEKGVF 0 1 -1.96E-13 0.014387446 Leu343Ala 20C 22 31 2 VELTTEKGVF 0.166666667 1 2.618890198 0.226541313 Leu343Ala 20C 22 31 2 VELTTEKGVF 0.333333333 1 2.79959845 0.209959172 Leu343Ala 20C 22 31 2 VELTTEKGVF 0.5 1 3.092904496 0.075107346 Leu343Ala 20C 22 31 2 VELTTEKGVF 0.75 1 3.102043368 0.081203722 Leu343Ala 20C 22 31 2 VELTTEKGVF 1 1 3.339826458 0.159968893 Leu343Ala 20C 22 31 2 VELTTEKGVF 3 1 4.181809658 0.036822108 Leu343Ala 20C 22 31 2 VELTTEKGVF 5 1 4.25819683 0.109247049 Leu343Ala 20C 22 31 2 VELTTEKGVF 10 1 4.76497312 0.113392584 Leu343Ala 20C 22 31 2 VELTTEKGVF 30 1 5.008618883 0.100955978 Leu343Ala 20C 22 31 2 VELTTEKGVF 60 1 5.091903315 0.017313706 Leu343Ala 20C 22 31 2 VELTTEKGVF 90 1 5.184843914 0.12582919 Leu343Ala 20C 22 31 2 VELTTEKGVF 120 1 5.252092214 0.190206915 Leu343Ala 20C 22 31 2 VELTTEKGVF 180 1 5.254678687 0.180696569 Leu343Ala 20C 22 31 2 VELTTEKGVF 240 1 5.207604877 0.144362172 Leu343Ala 25C 22 31 2 VELTTEKGVF 0 1 0 0.019264547 Leu343Ala 25C 22 31 2 VELTTEKGVF 0.166666667 1 2.813565405 0.053648104 Leu343Ala 25C 22 31 2 VELTTEKGVF 0.333333333 1 2.789942284 0.201180391 Leu343Ala 25C 22 31 2 VELTTEKGVF 0.5 1 3.225159486 0.116806554 Leu343Ala 25C 22 31 2 VELTTEKGVF 0.75 1 3.252920963 0.137046521 Leu343Ala 25C 22 31 2 VELTTEKGVF 1 1 3.36241499 0.123146785 Leu343Ala 25C 22 31 2 VELTTEKGVF 3 1 4.233366688 0.040967643 Leu343Ala 25C 22 31 2 VELTTEKGVF 5 1 4.337342905 0.226053603 Leu343Ala 25C 22 31 2 VELTTEKGVF 10 1 4.706518828 0.035602833 Leu343Ala 25C 22 31 2 VELTTEKGVF 30 1 5.056727282 0.173868629 Leu343Ala 25C 22 31 2 VELTTEKGVF 60 1 5.231572861 0.219713373 Leu343Ala 25C 22 31 2 VELTTEKGVF 90 1 5.145701955 0.075351201 Leu343Ala 25C 22 31 2 VELTTEKGVF 120 1 5.310374074 0.277507012 Leu343Ala 25C 22 31 2 VELTTEKGVF 180 1 5.062589954 0.086080822 Leu343Ala 25C 22 31 2 VELTTEKGVF 240 1 5.171739118 0.098517428 Leu343Ala 30C 22 31 2 VELTTEKGVF 0 1 0 0.012680461 Leu343Ala 30C 22 31 2 VELTTEKGVF 0.166666667 1 2.875468327 0.114124149 Leu343Ala 30C 22 31 2 VELTTEKGVF 0.333333333 1 3.196190987 0.020483822 Leu343Ala 30C 22 31 2 VELTTEKGVF 0.5 1 3.676757684 0.017801416 Leu343Ala 30C 22 31 2 VELTTEKGVF 0.75 1 3.625028222 0.017313706 Leu343Ala 30C 22 31 2 VELTTEKGVF 1 1 3.935922285 0.112661019 Leu343Ala 30C 22 31 2 VELTTEKGVF 3 1 4.453561762 0.017557561 Leu343Ala 30C 22 31 2 VELTTEKGVF 5 1 4.694276189 0.059012915 Leu343Ala 30C 22 31 2 VELTTEKGVF 10 1 4.932921437 0.060963755 Leu343Ala 30C 22 31 2 VELTTEKGVF 30 1 5.073970436 Leu343Ala 30C 22 31 2 VELTTEKGVF 60 1 5.009308609 0.202643521 Leu343Ala 30C 22 31 2 VELTTEKGVF 90 1 5.121216677 0.084373837 Leu343Ala 30C 22 31 2 VELTTEKGVF 120 1 5.150185175 Leu343Ala 30C 22 31 2 VELTTEKGVF 180 1 5.149323018 0.016338286 Leu343Ala 30C 22 31 2 VELTTEKGVF 240 1 5.074315299 0.087787807 Leu343Ala 40C 22 31 2 VELTTEKGVF 0 1 0 0.00438939 Leu343Ala 40C 22 31 2 VELTTEKGVF 0.166666667 1 3.630546031 0.30652576 Leu343Ala 40C 22 31 2 VELTTEKGVF 0.333333333 1 3.854534599 0.037553673 Leu343Ala 40C 22 31 2 VELTTEKGVF 0.5 1 4.069556727 0.292869878 Leu343Ala 40C 22 31 2 VELTTEKGVF 0.75 1 4.412350624 0.367489514 Leu343Ala 40C 22 31 2 VELTTEKGVF 1 1 4.490462111 0.287748923 Leu343Ala 40C 22 31 2 VELTTEKGVF 3 1 4.85360293 0.11948896 Leu343Ala 40C 22 31 2 VELTTEKGVF 5 1 5.002238916 0.015606721 Leu343Ala 40C 22 31 2 VELTTEKGVF 10 1 5.140184146 0.063890015 Leu343Ala 40C 22 31 2 VELTTEKGVF 30 1 5.251230057 0.198010276 Leu343Ala 40C 22 31 2 VELTTEKGVF 60 1 5.20898433 0.166065268 Leu343Ala 40C 22 31 2 VELTTEKGVF 90 1 5.213812413 0.018776837 Leu343Ala 40C 22 31 2 VELTTEKGVF 120 1 5.152426785 0.081203722 Leu343Ala 40C 22 31 2 VELTTEKGVF 180 1 5.15294408 0.056574365 Leu343Ala 40C 22 31 2 VELTTEKGVF 240 1 5.240539301 0.032188863 WT 10C 22 31 2 VELTTEKGVF 0 1 -1.96E-13 0.00146313 WT 10C 22 31 2 VELTTEKGVF 0.166666667 1 2.181948681 0.079984446 WT 10C 22 31 2 VELTTEKGVF 0.333333333 1 2.274716849 0.156067213 WT 10C 22 31 2 VELTTEKGVF 0.5 1 2.483531441 0.294333009 WT 10C 22 31 2 VELTTEKGVF 0.75 1 2.66510185 0.064865435 WT 10C 22 31 2 VELTTEKGVF 1 1 2.59216331 0.126560755 WT 10C 22 31 2 VELTTEKGVF 3 1 3.366380915 0.144606027 WT 10C 22 31 2 VELTTEKGVF 5 1 3.557090196 0.029018747 WT 10C 22 31 2 VELTTEKGVF 10 1 4.070936179 0.261168726 WT 10C 22 31 2 VELTTEKGVF 30 1 4.537880784 0.05925677 WT 10C 22 31 2 VELTTEKGVF 60 1 4.9141264 0.176794889 WT 10C 22 31 2 VELTTEKGVF 90 1 4.95792401 0.111441744 WT 10C 22 31 2 VELTTEKGVF 120 1 4.968097471 0.040967643 WT 10C 22 31 2 VELTTEKGVF 180 1 5.164324561 0.302867934 WT 10C 22 31 2 VELTTEKGVF 240 1 5.081557423 0.12582919 WT 20C 22 31 2 VELTTEKGVF 0 1 -1.96E-13 0.008047216 WT 20C 22 31 2 VELTTEKGVF 0.166666667 1 2.563367243 0.286529648 WT 20C 22 31 2 VELTTEKGVF 0.333333333 1 2.962028959 0.150946257 WT 20C 22 31 2 VELTTEKGVF 0.5 1 3.048934454 0.320181641 WT 20C 22 31 2 VELTTEKGVF 0.75 1 3.277406242 WT 20C 22 31 2 VELTTEKGVF 1 1 3.246713428 0.140948201 WT 20C 22 31 2 VELTTEKGVF 3 1 3.916609953 0.523069017 WT 20C 22 31 2 VELTTEKGVF 5 1 4.200949559 0.548673794 WT 20C 22 31 2 VELTTEKGVF 10 1 4.605991242 0.218737953 WT 20C 22 31 2 VELTTEKGVF 30 1 5.016895596 0.007315651 WT 20C 22 31 2 VELTTEKGVF 60 1 5.056382419 0.06803555 WT 20C 22 31 2 VELTTEKGVF 90 1 5.250195467 0.234832384 WT 20C 22 31 2 VELTTEKGVF 120 1 5.073625572 0.255803915 WT 20C 22 31 2 VELTTEKGVF 180 1 4.974477438 0.366757949 WT 20C 22 31 2 VELTTEKGVF 240 1 5.128803665 0.303599499 WT 25C 22 31 2 VELTTEKGVF 0 1 0 0.006340231 WT 25C 22 31 2 VELTTEKGVF 0.166666667 1 3.144461526 0.233613109 WT 25C 22 31 2 VELTTEKGVF 0.333333333 1 3.101870936 0.086568532 WT 25C 22 31 2 VELTTEKGVF 0.5 1 3.567263657 0.357491459 WT 25C 22 31 2 VELTTEKGVF 0.75 1 3.562090711 0.215567837 WT 25C 22 31 2 VELTTEKGVF 1 1 3.990410651 0.403336202 WT 25C 22 31 2 VELTTEKGVF 3 1 3.981099348 WT 25C 22 31 2 VELTTEKGVF 5 1 4.468735737 0.25019525 WT 25C 22 31 2 VELTTEKGVF 10 1 4.978443364 0.286773503 WT 25C 22 31 2 VELTTEKGVF 30 1 5.149840312 0.37748757 WT 25C 22 31 2 VELTTEKGVF 60 1 5.245712247 WT 25C 22 31 2 VELTTEKGVF 90 1 5.193982786 0.319937786 WT 25C 22 31 2 VELTTEKGVF 120 1 5.246919268 0.280189417 WT 25C 22 31 2 VELTTEKGVF 180 1 5.191741176 0.040236078 WT 25C 22 31 2 VELTTEKGVF 240 1 5.218640496 0.255316205 WT 30C 22 31 2 VELTTEKGVF 0 1 0 0.013412026 WT 30C 22 31 2 VELTTEKGVF 0.166666667 1 3.162394406 0.232881544 WT 30C 22 31 2 VELTTEKGVF 0.333333333 1 3.374140334 0.080716011 WT 30C 22 31 2 VELTTEKGVF 0.5 1 3.694000837 0.12534148 WT 30C 22 31 2 VELTTEKGVF 0.75 1 3.865052923 0.209227607 WT 30C 22 31 2 VELTTEKGVF 1 1 4.118527284 0.139972781 WT 30C 22 31 2 VELTTEKGVF 3 1 4.492013995 0.056574365 WT 30C 22 31 2 VELTTEKGVF 5 1 4.791872439 0.034871268 WT 30C 22 31 2 VELTTEKGVF 10 1 4.948440276 0.097785863 WT 30C 22 31 2 VELTTEKGVF 30 1 5.107249722 0.232149979 WT 30C 22 31 2 VELTTEKGVF 60 1 5.07897095 0.186305235 WT 30C 22 31 2 VELTTEKGVF 90 1 5.055347829 0.250926815 WT 30C 22 31 2 VELTTEKGVF 120 1 5.065176427 0.079009026 WT 30C 22 31 2 VELTTEKGVF 180 1 5.126217192 0.198010276 WT 30C 22 31 2 VELTTEKGVF 240 1 5.146908976 0.231174559 WT 40C 22 31 2 VELTTEKGVF 0 1 0 0.010973476 WT 40C 22 31 2 VELTTEKGVF 0.166666667 1 3.683482514 0.18362283 WT 40C 22 31 2 VELTTEKGVF 0.333333333 1 4.063349192 0.345786418 WT 40C 22 31 2 VELTTEKGVF 0.5 1 4.264404365 0.305794195 WT 40C 22 31 2 VELTTEKGVF 0.75 1 4.432869977 0.159725038 WT 40C 22 31 2 VELTTEKGVF 1 1 4.519258178 WT 40C 22 31 2 VELTTEKGVF 3 1 5.081557423 0.278726287 WT 40C 22 31 2 VELTTEKGVF 5 1 5.208639467 0.075595056 WT 40C 22 31 2 VELTTEKGVF 10 1 5.212088097 0.214104707 WT 40C 22 31 2 VELTTEKGVF 30 1 5.212432961 0.198985696 WT 40C 22 31 2 VELTTEKGVF 60 1 5.061382933 WT 40C 22 31 2 VELTTEKGVF 90 1 5.284509343 0.145825302 WT 40C 22 31 2 VELTTEKGVF 180 1 5.197086554 0.138753506 WT 40C 22 31 2 VELTTEKGVF 240 1 5.156047848 0.041699208 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 0 1 -2.28E-13 0.021295144 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 4.785212816 0.013912827 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 5.127730776 0.191088426 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 5.806341975 0.264911593 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 5.586094494 0.068712332 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 1 1 6.173755731 0.88559406 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 3 1 7.522545682 0.242764643 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 5 1 7.54844534 0.905753463 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 10 1 8.595072612 0.684000029 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 30 1 9.386718754 0.33390786 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 60 1 9.737468388 0.286490672 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 90 1 10.56485204 0.762082224 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 120 1 10.75397978 0.592288942 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 180 1 10.83308416 0.009085928 Leu343Ala 10C 32 49 2 RSIVPSGASTGVHEALEM 240 1 11.46290763 0.609041122 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 0 1 0 0.141115822 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 6.277354363 0.326241608 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 6.455640382 0.104204239 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 7.202313474 0.091427152 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 7.472553318 0.187965139 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 1 1 7.789773938 0.733688698 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 3 1 8.532230806 0.449753444 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 5 1 9.0968032 0.335611471 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 10 1 9.676433535 0.569858056 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 30 1 10.50622646 0.754699907 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 60 1 11.68978061 0.455716084 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 90 1 11.68355666 0.491207991 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 120 1 12.22343403 1.126939026 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 180 1 12.27101712 0.831930297 Leu343Ala 20C 32 49 2 RSIVPSGASTGVHEALEM 240 1 12.89541973 0.640557935 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 0 1 0 0.007098381 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 6.901756975 0.408582832 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 7.380398721 0.484109609 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 7.756847241 0.594560424 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 7.887550167 0.25071483 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 1 1 7.975086996 0.619830661 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 3 1 8.931768169 0.03293649 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 5 1 9.159042689 0.275417197 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 10 1 9.739476113 0.628916589 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 30 1 10.78650493 0.506540495 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 60 1 12.40433009 0.406595285 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 90 1 11.96704749 0.238505614 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 120 1 12.82093312 0.430161911 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 180 1 12.67236144 0.412557925 Leu343Ala 25C 32 49 2 RSIVPSGASTGVHEALEM 240 1 13.4419226 0.015900374 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 0 1 -2.28E-13 0.027257784 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 7.654252471 0.257813211 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 8.293913796 0.751292684 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 8.614547549 0.500577854 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 8.698671245 0.752996296 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 1 1 8.702887468 0.826535527 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 3 1 9.480077987 1.051696184 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 5 1 10.15206369 1.90037866 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 10 1 10.94872915 1.431317619 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 30 1 12.33305584 0.823980109 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 60 1 12.64947337 0.714948972 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 90 1 13.25098791 1.352099683 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 120 1 14.28918273 1.233130812 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.10783709 1.234834423 Leu343Ala 30C 32 49 2 RSIVPSGASTGVHEALEM 240 1 13.54411582 1.11472981 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 0 1 0 0.059342468 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 8.701482061 0.147362397 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 9.041992295 0.980996305 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 9.261637459 0.379053565 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 9.806734916 0.329932766 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 1 1 9.804124873 0.5959801 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 3 1 10.92323103 0.719775871 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 5 1 11.55425914 0.90234624 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 10 1 12.03249934 1.684871802 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 30 1 13.31643976 1.555681261 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 60 1 13.70393077 0.802117095 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 90 1 14.21569998 0.633175618 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 120 1 14.1753447 1.159875516 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.60615454 0.20102616 Leu343Ala 40C 32 49 2 RSIVPSGASTGVHEALEM 240 1 14.4634533 1.302694949 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 0 1 -2.28E-13 0.047701123 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 7.065185826 0.021011209 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 7.677542086 0.616139503 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 7.355302152 0.153608973 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 7.75443797 0.520453322 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 1 1 7.449665248 0.236518067 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 3 1 8.294516114 0.692518086 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 5 1 8.7085091 0.450037379 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 10 1 9.256618145 0.377917824 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 30 1 10.64897574 0.789340008 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 60 1 12.15256132 0.311476975 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 90 1 12.37622193 1.232562941 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 120 1 13.04619991 0.265763398 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.02572112 0.56787051 WT 10C 32 49 2 RSIVPSGASTGVHEALEM 240 1 12.78258556 0.693653827 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 0 1 0 0.005110835 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 8.232477398 0.594560424 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 8.33527294 0.183422175 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 8.565358276 0.319143226 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 8.775968674 0.076378584 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 1 1 8.492879387 0.121808224 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 3 1 9.334517892 0.042306353 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 5 1 9.973175355 0.038615195 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 10 1 11.18262916 0.01135741 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 30 1 13.29294937 0.648224187 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 60 1 13.04901073 0.257245341 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 90 1 12.82655475 0.315168133 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 120 1 13.35920431 0.01675218 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.03616129 0.279960161 WT 20C 32 49 2 RSIVPSGASTGVHEALEM 240 1 13.76456408 0.413409731 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 0 1 2.28E-13 0.070132008 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 8.281666671 0.002271482 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 8.475412176 0.013344957 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 8.862300868 0.235666261 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 8.807289191 0.210112089 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 1 1 9.524047174 0.319143226 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 3 1 9.821592084 0.314032392 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 5 1 10.24582447 0.367128284 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 10 1 12.09032183 0.101080951 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 30 1 12.66794444 0.076378584 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 60 1 12.49708701 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 90 1 13.07450884 0.576388567 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 120 1 13.24355933 0.085748447 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.52122775 0.255825665 WT 25C 32 49 2 RSIVPSGASTGVHEALEM 240 1 13.41521985 0.962824449 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 0 1 0 0.035207972 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 8.861497778 0.401768386 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 8.949636925 0.546859301 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 9.46220923 0.454580343 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 9.99485879 0.435556681 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 1 1 10.15467373 0.014196763 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 3 1 11.06156332 0.392682457 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 5 1 11.87870757 0.113290167 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 10 1 12.58542693 0.42902617 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 30 1 13.05684086 0.716368648 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 60 1 13.78042511 0.48467748 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 90 1 13.40738972 0.753848101 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 120 1 13.65293454 0.11130262 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 180 1 13.90088863 0.601090934 WT 30C 32 49 2 RSIVPSGASTGVHEALEM 240 1 13.89908168 0.597967647 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 0 1 -2.28E-13 0.049404734 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 0.166666667 1 9.442734293 0.487232897 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 0.333333333 1 9.562595502 0.001419676 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 0.5 1 10.33858139 0.160139484 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 0.75 1 10.61624981 0.610176863 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 1 1 10.68190244 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 3 1 12.14653814 0.38473227 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 5 1 12.95384454 0.126067253 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 10 1 13.36362131 0.098525533 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 30 1 13.42485693 0.82511585 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 60 1 13.2268952 1.319731064 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 90 1 13.92277284 0.637150712 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 180 1 14.62166207 0.230839362 WT 40C 32 49 2 RSIVPSGASTGVHEALEM 240 1 14.91900621 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 0 1 -4.03E-13 0.011521671 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 3.271926829 0.231435306 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 3.315495845 0.027551822 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 3.512087746 0.006011307 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 3.328956191 0.096681849 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 1 1 3.620124736 0.844087643 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 3 1 3.988513163 0.36318311 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 5 1 3.812466002 0.053099875 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 10 1 4.882563539 0.18484768 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 30 1 5.338089998 0.091672426 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 60 1 5.338444217 0.034064071 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 90 1 5.423102712 0.358173688 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 120 1 5.601983631 0.470885688 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 180 1 5.80991056 0.043581973 Leu343Ala 10C 48 63 3 EMRDGDKSKWMGKGVL 240 1 6.022088125 0.085160178 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 0 1 4.03E-13 0.152286435 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 3.901729351 0.494429972 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 3.800068314 0.360678399 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 4.254886334 0.062116835 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 4.073525877 0.119224249 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 1 1 4.543221122 0.406765084 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 3 1 5.248826648 0.187352391 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 5 1 5.263349653 0.312087004 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 10 1 5.744379926 0.173826951 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 30 1 6.070970436 0.034565013 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 60 1 6.486470075 0.035065955 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 90 1 6.909054108 0.154791146 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 120 1 7.407086924 0.145774186 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 180 1 7.807709338 0.0901696 Leu343Ala 20C 48 63 3 EMRDGDKSKWMGKGVL 240 1 8.111275571 0.043581973 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 0 1 0 0.186851448 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 4.186167723 0.147777955 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 3.73453768 0.257484302 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 4.280390148 0.108704462 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 4.237883791 0.039574435 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 1 1 4.390198237 0.455356479 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 3 1 5.30621023 0.149781724 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 5 1 5.191443066 0.425299946 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 10 1 5.535744558 0.093676195 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 30 1 6.261186383 0.335130346 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 60 1 6.833251104 0.716347376 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 90 1 7.250876062 0.604637261 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 120 1 7.941604363 0.531499697 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 180 1 8.286968513 0.600128781 Leu343Ala 25C 48 63 3 EMRDGDKSKWMGKGVL 240 1 8.624185612 0.792490594 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 0 1 0 0.054602702 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 3.799714094 0.050094222 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 3.978949233 0.076143218 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 4.645590598 0.048090453 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 4.559515226 0.122730844 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 1 1 5.012916367 0.002504711 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 3 1 5.30621023 0.093676195 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 5 1 5.883942465 0.384723626 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 10 1 6.375599328 0.48491207 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 30 1 7.960023784 0.05861024 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 60 1 8.656419599 0.189857102 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 90 1 9.49521171 0.199875946 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 120 1 10.2543044 0.561055288 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 180 1 10.18629423 Leu343Ala 30C 48 63 3 EMRDGDKSKWMGKGVL 240 1 10.85647779 0.371699128 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 0 1 0 0.040075378 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 4.964388276 0.039574435 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 5.16771035 0.39023399 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 5.391577163 0.296056853 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 6.135438411 0.326113386 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 1 1 6.215846269 0.098184675 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 3 1 7.176844157 0.200877831 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 5 1 7.996508407 0.137759111 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 10 1 9.191645478 0.396245297 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 30 1 11.361595 0.342143537 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 60 1 12.36474503 0.281028586 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 90 1 12.62509646 0.004007538 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 120 1 12.92653738 0.118723306 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 180 1 12.68177161 0.62016647 Leu343Ala 40C 48 63 3 EMRDGDKSKWMGKGVL 240 1 12.6853138 0.160301511 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 0 1 0 0.113212942 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 3.807506927 0.303070044 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 3.891102762 0.231936248 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 3.993826458 0.052598933 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 4.438726328 0.620667412 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 1 1 4.17943755 0.073638506 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 3 1 4.924715676 0.330120924 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 5 1 5.056131163 0.33162375 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 10 1 5.517325136 0.044082915 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 30 1 5.670348021 0.030056533 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 60 1 6.388351235 0.375205724 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 90 1 6.872215265 0.001502827 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 120 1 7.007172948 0.011020729 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 180 1 7.784685061 0.362181226 WT 10C 48 63 3 EMRDGDKSKWMGKGVL 240 1 7.702860324 0.685288958 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 0 1 4.03E-13 0.025047111 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 3.980366112 0.368192532 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 4.162789227 0.0901696 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 4.437309449 0.114715769 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 4.81171961 0.234440959 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 1 1 4.285349223 0.601130665 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 3 1 5.191797286 0.66074279 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 5 1 5.199235898 1.090050273 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 10 1 6.088681418 0.229932479 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 30 1 6.862651335 0.952792105 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 60 1 8.945817046 0.587605225 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 90 1 9.741394361 0.629684372 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 120 1 9.857224184 0.890675269 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 180 1 10.87312612 0.717349261 WT 20C 48 63 3 EMRDGDKSKWMGKGVL 240 1 11.75513302 0.29555591 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 0 1 -4.03E-13 0.152286435 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 4.909838451 0.488418666 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 4.481941124 0.757925581 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 5.518742015 0.308580408 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 5.549204904 0.874645118 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 1 1 5.785823624 0.470885688 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 3 1 6.222930662 0.68328519 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 5 1 6.124457602 0.532000639 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 10 1 7.801333385 0.079148871 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 30 1 9.882373779 0.298060622 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 60 1 11.0063127 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 90 1 11.67507938 0.169318471 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 120 1 11.80507799 0.704825705 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 180 1 12.62651334 WT 25C 48 63 3 EMRDGDKSKWMGKGVL 240 1 12.28965046 0.039574435 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 0 1 -4.03E-13 0.021540516 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 4.521967944 0.276019164 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 4.975014865 0.353665208 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 5.47411034 0.359175573 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 5.529014384 0.007013191 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 1 1 5.973205815 0.159299626 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 3 1 6.675977584 0.429808426 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 5 1 7.444988425 0.066625315 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 10 1 8.930585602 0.164809991 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 30 1 11.1235594 0.196369351 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 60 1 11.01091756 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 90 1 12.15363012 0.42179335 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 120 1 12.29744329 0.300565333 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 180 1 12.31409162 0.782972692 WT 30C 48 63 3 EMRDGDKSKWMGKGVL 240 1 12.28433717 0.918227091 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 0 1 0 0.113713884 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 0.166666667 1 5.894214835 0.01352544 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 0.333333333 1 6.405707997 0.401254719 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 0.5 1 7.414525536 0.188855217 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 0.75 1 7.950814073 0.391235875 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 1 1 8.401027238 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 3 1 10.69743317 0.270007857 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 5 1 11.12391362 0.299062506 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 10 1 12.04346781 1.002385384 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 30 1 12.75438663 0.347653901 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 60 1 12.85073437 0.587104283 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 90 1 13.13871494 0.14877984 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 180 1 13.1217124 0.717850203 WT 40C 48 63 3 EMRDGDKSKWMGKGVL 240 1 12.5733804 0.107702578 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 0 1 -1.84E-13 0.087516979 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.16963715 0.060024734 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.136427231 0.159225917 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.278500885 0.31570261 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.052511436 0.089578897 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 1 1 1.310252808 0.465535343 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 3 1 1.393358605 0.252241345 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 5 1 1.133025239 0.169993713 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 10 1 1.802731608 0.462786119 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 30 1 1.981579172 0.520978037 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 60 1 2.054316995 0.11569653 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 90 1 2.083314924 0.207108243 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 120 1 2.213238608 0.140668652 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 180 1 2.206920623 0.12027857 Leu343Ala 10C 64 77 2 HAVKNVNDVIAPAF 240 1 2.45445602 0.043758489 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 0 1 -1.84E-13 0.051089755 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.350752709 0.252241345 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.386068623 0.252241345 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.565888185 0.009851388 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.359338688 0.09209902 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 1 1 1.470956416 0.154185672 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 3 1 1.989355153 0.092786326 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 5 1 1.809697591 0.184198039 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 10 1 2.177760694 0.189238284 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 30 1 2.358390254 0.112489101 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 60 1 2.223444583 0.065981387 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 90 1 2.388360181 0.2359751 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 120 1 2.51585387 0.406656119 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 180 1 2.571743734 0.241473549 Leu343Ala 20C 64 77 2 HAVKNVNDVIAPAF 240 1 2.438256059 0.282253712 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 0 1 0 0.061857551 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.520042297 0.009851388 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.252256949 0.031616081 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.59245612 0.013287918 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.382666631 0.048798734 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 1 1 1.341194732 0.054297183 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 3 1 1.870447443 0.10676155 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 5 1 1.743115753 0.090266203 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 10 1 1.980283175 0.131046366 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 30 1 2.31076237 0.09668106 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 60 1 2.258112498 0.089578897 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 90 1 2.235594553 0.01008049 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 120 1 2.232030562 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 180 1 2.266698477 0.155331182 Leu343Ala 25C 64 77 2 HAVKNVNDVIAPAF 240 1 2.258436498 0.02588853 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 0 1 0 0.083622244 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.241888974 0.184427141 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.277690887 0.158996815 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.758829715 0.020619184 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.363388678 0.148687223 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 1 1 1.491692366 0.243077263 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 3 1 1.648021985 0.033448898 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 5 1 1.91661733 0.114092815 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 10 1 2.053020998 0.172742937 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 30 1 2.615969626 0.782383463 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 60 1 2.048971008 0.326012201 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 90 1 2.253738509 0.301269181 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 120 1 2.178084693 0.082476734 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 180 1 2.358876253 0.31799363 Leu343Ala 30C 64 77 2 HAVKNVNDVIAPAF 240 1 2.217126598 0.325553997 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 0 1 0 0.00229102 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.517936302 0.16403706 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.628096033 0.049027836 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.562486193 0.469430078 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.635062016 0.43369016 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 1 1 1.66648994 0.530829424 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 3 1 1.925203309 0.290959589 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 5 1 2.047675011 0.174575754 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 10 1 2.369406227 0.391306282 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 30 1 1.991299148 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 60 1 2.332308318 0.496005914 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 90 1 2.236242552 0.049027836 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 120 1 2.117496841 0.265529263 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 180 1 2.470331981 0.270111304 Leu343Ala 40C 64 77 2 HAVKNVNDVIAPAF 240 1 2.362278245 0.228185631 WT 10C 64 77 2 HAVKNVNDVIAPAF 0 1 1.84E-13 0.042383877 WT 10C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.12443926 0.03482351 WT 10C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.025781501 0.109739877 WT 10C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.32985476 0.172284733 WT 10C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.277042889 0.026575837 WT 10C 64 77 2 HAVKNVNDVIAPAF 1 1 1.099167322 0.013287918 WT 10C 64 77 2 HAVKNVNDVIAPAF 3 1 1.442282486 0.193820325 WT 10C 64 77 2 HAVKNVNDVIAPAF 5 1 1.425272527 0.123027795 WT 10C 64 77 2 HAVKNVNDVIAPAF 10 1 1.571234172 0.398637548 WT 10C 64 77 2 HAVKNVNDVIAPAF 30 1 1.83448353 0.146854407 WT 10C 64 77 2 HAVKNVNDVIAPAF 60 1 2.084772921 0.033448898 WT 10C 64 77 2 HAVKNVNDVIAPAF 90 1 2.052373 0.114092815 WT 10C 64 77 2 HAVKNVNDVIAPAF 120 1 1.987411158 0.065294081 WT 10C 64 77 2 HAVKNVNDVIAPAF 180 1 2.313840363 0.520519832 WT 10C 64 77 2 HAVKNVNDVIAPAF 240 1 2.145684772 0.143417876 WT 20C 64 77 2 HAVKNVNDVIAPAF 0 1 1.84E-13 0.017869959 WT 20C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.219533029 0.269423998 WT 20C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.434992504 0.039405551 WT 20C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.397570595 0.364501344 WT 20C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.328072764 WT 20C 64 77 2 HAVKNVNDVIAPAF 1 1 1.16153717 0.115925632 WT 20C 64 77 2 HAVKNVNDVIAPAF 3 1 1.677181914 0.468971874 WT 20C 64 77 2 HAVKNVNDVIAPAF 5 1 1.71978781 0.401386772 WT 20C 64 77 2 HAVKNVNDVIAPAF 10 1 2.158158742 0.182823427 WT 20C 64 77 2 HAVKNVNDVIAPAF 30 1 2.299746397 0.084767754 WT 20C 64 77 2 HAVKNVNDVIAPAF 60 1 2.365518237 0.252928651 WT 20C 64 77 2 HAVKNVNDVIAPAF 90 1 2.722565367 WT 20C 64 77 2 HAVKNVNDVIAPAF 120 1 2.320158347 0.203442611 WT 20C 64 77 2 HAVKNVNDVIAPAF 180 1 2.252280513 0.184885345 WT 20C 64 77 2 HAVKNVNDVIAPAF 240 1 2.460288006 0.216959631 WT 25C 64 77 2 HAVKNVNDVIAPAF 0 1 -1.84E-13 0.007789469 WT 25C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.761259709 0.042612979 WT 25C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.377320644 0.044904 WT 25C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.775839673 0.400928568 WT 25C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.480028394 0.433919262 WT 25C 64 77 2 HAVKNVNDVIAPAF 1 1 1.856515477 0.428879017 WT 25C 64 77 2 HAVKNVNDVIAPAF 3 1 2.061444977 0.530829424 WT 25C 64 77 2 HAVKNVNDVIAPAF 5 1 1.86736945 0.065752285 WT 25C 64 77 2 HAVKNVNDVIAPAF 10 1 2.285166432 0.286835753 WT 25C 64 77 2 HAVKNVNDVIAPAF 30 1 2.628281596 0.498067833 WT 25C 64 77 2 HAVKNVNDVIAPAF 60 1 2.601065663 WT 25C 64 77 2 HAVKNVNDVIAPAF 90 1 2.592479684 0.546866567 WT 25C 64 77 2 HAVKNVNDVIAPAF 120 1 2.590697688 0.169993713 WT 25C 64 77 2 HAVKNVNDVIAPAF 180 1 2.447328037 0.359461099 WT 25C 64 77 2 HAVKNVNDVIAPAF 240 1 2.549549788 0.483405302 WT 30C 64 77 2 HAVKNVNDVIAPAF 0 1 0 0.019015469 WT 30C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.3543167 0.303101997 WT 30C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.331798755 0.054068081 WT 30C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.463666434 0.054068081 WT 30C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.440986489 0.199318774 WT 30C 64 77 2 HAVKNVNDVIAPAF 1 1 1.455404454 0.046965918 WT 30C 64 77 2 HAVKNVNDVIAPAF 3 1 1.575446162 0.052235265 WT 30C 64 77 2 HAVKNVNDVIAPAF 5 1 1.93735328 0.202067998 WT 30C 64 77 2 HAVKNVNDVIAPAF 10 1 2.033581045 0.089349795 WT 30C 64 77 2 HAVKNVNDVIAPAF 30 1 2.263134486 0.496464118 WT 30C 64 77 2 HAVKNVNDVIAPAF 60 1 2.123976825 0.110427183 WT 30C 64 77 2 HAVKNVNDVIAPAF 90 1 2.200440639 0.309745957 WT 30C 64 77 2 HAVKNVNDVIAPAF 120 1 2.160912735 0.194736733 WT 30C 64 77 2 HAVKNVNDVIAPAF 180 1 2.164152727 0.306996732 WT 30C 64 77 2 HAVKNVNDVIAPAF 240 1 2.221986586 0.387411548 WT 40C 64 77 2 HAVKNVNDVIAPAF 0 1 0 0.021535592 WT 40C 64 77 2 HAVKNVNDVIAPAF 0.166666667 1 1.614164067 0.067355999 WT 40C 64 77 2 HAVKNVNDVIAPAF 0.333333333 1 1.632794022 0.586272118 WT 40C 64 77 2 HAVKNVNDVIAPAF 0.5 1 1.626962036 0.297145344 WT 40C 64 77 2 HAVKNVNDVIAPAF 0.75 1 1.63360402 0.267591181 WT 40C 64 77 2 HAVKNVNDVIAPAF 1 1 1.67053993 WT 40C 64 77 2 HAVKNVNDVIAPAF 3 1 2.433072072 0.484092608 WT 40C 64 77 2 HAVKNVNDVIAPAF 5 1 2.539991812 0.141355958 WT 40C 64 77 2 HAVKNVNDVIAPAF 10 1 2.528651839 0.10332502 WT 40C 64 77 2 HAVKNVNDVIAPAF 30 1 2.564129753 0.338154609 WT 40C 64 77 2 HAVKNVNDVIAPAF 60 1 2.717381379 WT 40C 64 77 2 HAVKNVNDVIAPAF 90 1 2.690489445 0.465993547 WT 40C 64 77 2 HAVKNVNDVIAPAF 180 1 2.61046164 0.080185714 WT 40C 64 77 2 HAVKNVNDVIAPAF 240 1 3.070864518 0.133337387 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 0 1 0 0.002895546 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 4.135528211 0.291967543 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 4.204800612 0.029920641 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 4.599277929 0.264459857 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 4.245067327 0.135125474 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 1 1 3.871747098 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 3 1 5.143902323 0.776971477 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 5 1 4.815626216 0.417923788 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 10 1 6.342348983 0.229713306 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 30 1 7.564682678 0.362908417 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 60 1 8.596602581 0.488382071 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 90 1 9.437426206 0.632194183 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 120 1 10.00730261 0.666940734 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 180 1 9.851354395 0.46280475 Leu343Ala 10C 78 94 3 VKANIDVKDQKAVDDFL 240 1 10.65157006 0.475834706 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 0 1 0 0.004343319 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 4.250185978 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 4.929260253 0.243225854 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 5.322372597 0.556909991 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 5.097493226 0.451222566 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 1 1 5.465694806 0.527954532 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 3 1 6.920756468 0.365803963 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 5 1 7.529193369 0.076731966 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 10 1 8.57408052 0.504307574 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 30 1 10.11547675 0.48259098 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 60 1 10.77373518 0.370147281 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 90 1 10.85051494 0.18241939 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 120 1 11.09757512 0.252877673 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 180 1 11.33337428 0.255290628 Leu343Ala 20C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.20233683 0.235021807 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 0 1 0 0.013512547 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 5.201231206 0.304032317 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 4.7753595 0.052119826 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 5.530531043 0.235986989 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 5.281082151 0.165046115 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 1 1 5.413825816 0.574765857 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 3 1 7.051452673 0.154429114 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 5 1 7.716194727 0.214270395 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 10 1 8.792476267 0.168906843 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 30 1 10.40280366 0.049706871 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 60 1 11.23066003 0.302584544 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 90 1 10.90067771 0.084936012 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 120 1 11.34156412 0.118717381 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 180 1 11.10269378 0.554979627 Leu343Ala 25C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.31255844 0.017855866 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 0 1 0 0.003378137 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 4.971233185 0.086383785 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 5.208056073 0.318027456 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 6.247142087 0.520233076 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 5.940364311 0.243708445 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 1 1 6.523207961 0.818956893 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 3 1 8.231130949 0.865768218 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 5 1 9.248377388 0.958908277 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 10 1 10.26016393 0.728712379 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 30 1 11.39718679 0.321405592 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 60 1 10.98121114 0.094105241 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 90 1 11.32825563 0.481625798 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 120 1 11.80429011 0.890380358 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 180 1 11.75446858 0.770697795 Leu343Ala 30C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.84421558 0.412132697 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 0 1 3.88E-13 0.019303639 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 6.220866349 0.186280118 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 6.701678235 0.530367487 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 7.16713417 0.119199972 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 8.274810098 0.436744837 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 1 1 8.462493943 0.003378137 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 3 1 10.06224279 0.259151356 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 5 1 10.7290323 0.346982914 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 10 1 11.02489029 0.422749698 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 30 1 11.51013834 0.150568386 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 60 1 12.13358995 0.025577322 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 90 1 12.2260669 0.413097879 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 120 1 12.54581192 0.247569173 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 180 1 12.7959433 0.28086795 Leu343Ala 40C 78 94 3 VKANIDVKDQKAVDDFL 240 1 13.13821038 0.346017732 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 0 1 -3.88E-13 0.008204047 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 4.049193642 0.095070423 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 4.142694321 0.032333596 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 4.651146919 0.159737614 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 4.710182019 0.194966756 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 1 1 4.308538591 0.004343319 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 3 1 5.226141971 0.122578109 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 5 1 5.243204139 0.303067135 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 10 1 6.12736567 0.284246087 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 30 1 7.862246883 0.412615288 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 60 1 9.422752742 0.109065561 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 90 1 9.793001781 0.163598342 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 120 1 9.970107082 0.411167515 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 180 1 10.59492366 0.155876886 WT 10C 78 94 3 VKANIDVKDQKAVDDFL 240 1 10.51985013 0.141399157 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 0 1 -3.88E-13 0.028955459 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 4.455273234 0.286659042 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 4.907761921 0.020268821 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 4.917657979 0.44350111 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 4.921411656 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 1 1 4.898548351 0.050672053 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 3 1 6.474068918 0.547258171 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 5 1 7.092060632 0.568009583 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 10 1 8.598308798 0.192553801 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 30 1 10.13765757 0.321405592 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 60 1 10.61505702 0.378833919 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 90 1 11.06754571 0.082523058 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 120 1 10.73210349 0.183384572 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 180 1 10.62017567 0.386072784 WT 20C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.13238195 0.16263316 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 0 1 3.88E-13 0.012547365 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 5.442831501 0.273146495 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 5.030950772 0.041020233 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 5.804208213 0.540984488 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 5.65235492 0.762493748 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 1 1 6.391146782 0.673214417 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 3 1 7.782737181 0.752359337 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 5 1 7.868389263 0.432401518 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 10 1 9.810405192 0.196414529 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 30 1 10.72288992 0.50237721 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 60 1 10.87133078 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 90 1 11.04161121 0.324783729 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 120 1 11.32177201 0.242260672 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 180 1 11.61660627 0.402480877 WT 25C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.69099732 0.050189462 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 0 1 0 0.031368414 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 5.203278666 0.269768358 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 5.249346519 0.055980554 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 5.765306471 0.00482591 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 6.195273097 0.120647745 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 1 1 6.884925916 0.013995138 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 3 1 8.454645346 0.131747337 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 5 1 9.434696259 0.460874386 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 10 1 10.16563952 0.235986989 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 30 1 10.76349788 0.012064774 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 60 1 11.03137391 0.090727104 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 90 1 11.11327232 0.046328734 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 120 1 11.34156412 0.275559449 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 180 1 11.78586297 0.023646958 WT 30C 78 94 3 VKANIDVKDQKAVDDFL 240 1 11.98276039 0.001930364 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 0 1 3.88E-13 0.005791092 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 0.166666667 1 6.087098954 0.059841281 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 0.333333333 1 6.887997106 0.485003935 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 0.5 1 7.532605803 0.4333667 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 0.75 1 8.104870908 0.090244513 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 1 1 8.995857306 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 3 1 10.63280168 0.067080146 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 5 1 11.00339196 0.227300351 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 10 1 11.25079339 0.111961107 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 30 1 11.66949899 0.02461214 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 60 1 12.13086 0.028472868 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 90 1 12.47585703 0.249016946 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 180 1 13.23478225 0.122578109 WT 40C 78 94 3 VKANIDVKDQKAVDDFL 240 1 12.99420569 0.642328594 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 0 1 2.25E-13 0.02156813 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 4.77771331 0.305034984 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 4.752162982 0.095796111 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.019550137 0.556289695 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 4.716511361 0.019607391 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.023709493 1.035550354 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 3 1 5.572744453 0.816507785 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 5 1 4.959932705 0.395228983 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 10 1 5.91797098 0.241451016 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 30 1 6.10949941 0.526878608 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 60 1 6.29805687 0.40363215 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 90 1 6.463836907 0.632478415 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 120 1 6.881753128 0.737237904 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 180 1 6.501073044 0.63976116 Leu343Ala 10C 94 112 2 LISLDGTANKSKLGANAIL 240 1 7.508429395 0.49186541 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 0 1 -2.25E-13 0.02156813 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.075800472 0.224084469 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.092437895 0.769730152 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.555908965 0.064984496 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.162948879 0.008403168 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.51788057 0.025209503 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 3 1 6.407586572 0.01904718 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 5 1 6.397683344 1.030228348 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 10 1 6.452349162 0.271702419 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 30 1 6.763310521 0.005602112 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 60 1 7.312345481 0.267780941 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 90 1 7.438512605 0.009803696 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 120 1 8.008740473 0.070026397 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 180 1 8.499544452 0.215681302 Leu343Ala 20C 94 112 2 LISLDGTANKSKLGANAIL 240 1 8.569659306 0.232487637 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 0 1 0 0.019327285 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.382206346 0.206998028 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 4.823664288 0.387386026 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.700892223 0.137811949 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.209890179 0.055741012 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.329521173 0.243411755 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 3 1 6.167136196 0.361336207 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 5 1 6.006901968 0.47533918 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 10 1 6.09820973 0.231367215 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 30 1 6.872047965 0.145935011 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 60 1 7.72669654 0.315959102 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 90 1 7.457526803 0.014845596 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 120 1 8.257311495 0.419318063 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 180 1 8.366048939 0.406713312 Leu343Ala 25C 94 112 2 LISLDGTANKSKLGANAIL 240 1 8.694638043 0.057981856 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 0 1 -2.25E-13 0.069466185 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.231677281 0.082351042 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.246928252 0.029411087 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 6.150102644 0.216521618 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.497083791 0.172545041 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.820721281 0.62071398 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 3 1 6.008684549 0.264699779 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 5 1 6.32321107 0.569454658 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 10 1 6.602878228 0.330804698 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 30 1 8.087570168 0.305034984 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 60 1 8.015078539 0.236689221 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 90 1 8.762772252 0.734997059 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 120 1 9.271005912 1.255433239 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 180 1 9.344487864 0.522396919 Leu343Ala 30C 94 112 2 LISLDGTANKSKLGANAIL 240 1 9.934126058 0.733316426 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 0 1 2.25E-13 0.051819534 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.839735479 0.003081161 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.994621965 0.344249766 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.950651633 0.452370522 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 6.14772587 0.287948543 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 1 1 6.203976205 0.322121425 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 3 1 6.499290463 0.010363907 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 5 1 7.024161546 0.219882885 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 10 1 7.622316517 0.233888165 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 30 1 8.931721321 0.315398891 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 60 1 10.20785128 0.060222701 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 90 1 10.49286618 0.843678027 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 120 1 11.15479794 0.898578722 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 180 1 11.5439948 0.381783915 Leu343Ala 40C 94 112 2 LISLDGTANKSKLGANAIL 240 1 12.01974587 1.145351744 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 0 1 0 0.012884857 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 4.602426175 0.053500167 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 4.686405548 0.071426925 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.07698886 0.235008587 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.173842429 0.395509088 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 1 1 4.627382309 0.010924118 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 3 1 5.388940541 0.149016172 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 5 1 5.448756038 0.424079858 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 10 1 5.879150327 0.104479384 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 30 1 5.996404546 0.524637764 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 60 1 6.823126018 0.185710004 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 90 1 6.939984109 0.079830092 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 120 1 7.134483506 0.437805032 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 180 1 8.125004369 0.070026397 WT 10C 94 112 2 LISLDGTANKSKLGANAIL 240 1 8.128569532 0.154058073 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 0 1 0 0.035853515 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 4.865059781 0.443967355 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.332492142 0.014845596 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.209097921 0.512033012 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.774572239 1.006979584 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.04965595 0.323521953 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 3 1 5.723075454 0.726313786 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 5 1 5.609188332 0.69242101 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 10 1 6.432740771 0.359655573 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 30 1 7.025944127 0.416517007 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 60 1 7.970712077 0.543124732 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 90 1 8.960044553 0.152377439 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 120 1 8.930136805 0.139772688 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 180 1 9.263479459 0.120445402 WT 20C 94 112 2 LISLDGTANKSKLGANAIL 240 1 10.06603705 0.174225675 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 0 1 0 0.036133621 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.427365066 0.325762797 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.150668876 0.058822173 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 5.416075386 0.150136594 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 5.518276699 0.361336207 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 1 1 5.934410339 0.250134289 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 3 1 5.698515448 0.24369186 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 5 1 6.427393028 0.46777633 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 10 1 7.032678323 0.156298917 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 30 1 8.587287052 0.220443097 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 60 1 9.69704278 0.339487971 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 90 1 10.28509646 0.192992749 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 120 1 10.67072816 0.621834402 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 180 1 11.44773542 0.334165965 WT 30C 94 112 2 LISLDGTANKSKLGANAIL 240 1 11.52913996 0.598305533 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 0 1 2.25E-13 0.001120422 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 0.166666667 1 5.794774824 0.028850875 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 0.333333333 1 5.985907124 0.626876303 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 0.5 1 6.135049738 0.306155406 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 0.75 1 6.091079406 0.116243818 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 1 1 6.416103348 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 3 1 7.7492759 0.204196973 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 5 1 8.267808917 0.238930065 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 10 1 9.206832995 0.197754544 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 30 1 10.84522303 0.296911922 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 60 1 11.60262191 0.86888753 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 90 1 12.1677001 0.868607424 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 180 1 13.63377397 0.639481054 WT 40C 94 112 2 LISLDGTANKSKLGANAIL 240 1 13.12157902 1.552065055 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 -4.48E-13 0.028439548 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 4.849221328 0.166733821 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 4.795200892 0.404845332 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.360246766 0.694259557 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 4.620521672 0.126583871 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 4.935969473 0.998730014 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.521913764 1.009882778 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 4.523127163 0.437188348 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 5.943430889 0.873261419 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 5.661105107 1.418631578 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 5.446600602 0.051302714 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 5.809759883 0.833669107 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 6.331825994 0.828092725 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 6.267947815 0.103163067 Leu343Ala 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 7.058144557 0.112085278 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 4.48E-13 0.00613402 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.568836625 0.818612876 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.62403999 0.585520109 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.895325099 0.130487339 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.301100303 0.116546384 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.78334113 0.007806935 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 6.774635846 0.126026233 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.698170223 0.293317693 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 6.487578346 0.700393578 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 6.518334507 0.032343016 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 6.694985276 0.185135882 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 7.204433475 0.090337388 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 7.750552481 0.6686082 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 8.186659067 0.17342548 Leu343Ala 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 8.081772673 0.263762868 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 0 0.015056231 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.894930789 0.174540756 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.060571355 0.17342548 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.929630048 0.036246483 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.347234544 0.010037488 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.479722621 0.373617593 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 6.328671516 0.695374834 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.853922575 0.125468595 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 5.708422277 0.31896905 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 6.543176021 0.116546384 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 6.989140351 0.301124627 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 7.089689337 0.007249297 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 7.97491473 1.057282025 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 7.647637636 0.497413273 Leu343Ala 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 7.711515816 0.168406736 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 0 0.035688845 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.080681152 0.467858449 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.111043003 0.272127441 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 6.336952021 0.171194927 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.247868487 0.554850008 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.521125145 0.548715988 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.442263194 0.036804121 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.825532273 0.337928748 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 5.894930789 0.149447037 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 6.630712786 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 6.844034362 0.657455436 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 7.608206661 0.354100256 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 7.64132868 0.416555734 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 8.179561491 0.239784425 Leu343Ala 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 8.071914929 0.542581967 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 -4.48E-13 0.04349578 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.89335355 0.150562314 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.919772304 0.029554825 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.805028166 1.138697202 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.994296847 0.642399205 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.731292242 1.236283887 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.975764289 0.479011213 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 6.445781513 0.161157439 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 7.229274989 0.644629758 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 8.120415029 1.292605345 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 8.511570302 1.063973683 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 8.582151748 0.439976539 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 8.45202953 0.128814424 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 9.511539833 0.798537901 Leu343Ala 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 9.477629194 0.358003724 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 0 0.029554825 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 4.81333914 0.022305528 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 4.694257595 0.281049652 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.435559929 0.327891261 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.223815592 0.171194927 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 4.782977289 0.110970002 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.30543771 0.490721615 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.299917374 0.519718801 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 5.549909757 0.693701919 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 5.398889122 0.654109607 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 6.001000113 0.373617593 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 5.665836824 0.035688845 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 5.680031975 0.284395481 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 6.839696955 1.073453533 WT 10C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 6.884648266 0.142197741 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 -4.48E-13 0.007806935 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 4.907184861 0.666377648 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.348811783 0.087549197 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.133124349 1.172155494 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.085807179 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 4.459248983 0.036246483 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.678060426 1.144273584 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.048347752 1.710276356 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 5.866146178 0.10539362 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 5.938699172 0.054090905 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 6.770298438 0.886644736 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 8.248565698 0.394250207 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 7.086929169 0.46451262 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 7.198518829 0.15167759 WT 20C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 7.574690332 0.511911866 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 4.48E-13 0.019517337 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 6.187508625 0.714334533 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.166246368 0.319526688 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 6.221419264 1.0277272 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.398494812 1.078472276 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 6.232854247 1.410824643 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 6.491521444 1.425323236 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.437137168 0.506335484 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 6.219053405 0.980885592 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 7.41893798 1.001518205 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 7.616881475 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 8.048650654 0.998730014 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 8.203614386 0.296663522 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 7.897235709 0.761176141 WT 25C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 8.438622998 1.050590366 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 4.48E-13 0.005576382 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.466710399 0.741658804 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.402832219 0.122680404 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.341714208 0.274915632 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.346051615 0.748350463 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.606296051 0.259859401 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 5.282962055 0.158369248 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 5.937516243 0.672511668 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 6.080650682 0.073050604 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 7.18156351 1.010998054 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 7.206799334 0.07751171 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 7.622796122 0.457263323 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 120 1 7.756861437 0.080299901 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 8.02341483 0.393692568 WT 30C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 8.496980842 0.524737545 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0 1 0 0.044611056 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.166666667 1 5.468681948 0.500201464 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.333333333 1 5.649275814 1.735927713 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.5 1 5.571596793 0.73050604 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 0.75 1 5.267978284 0.738312975 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 1 1 5.435954238 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 3 1 6.808546484 1.04334107 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 5 1 7.294336099 0.020632613 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 10 1 7.308925559 0.335698196 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 30 1 8.026963617 0.821401067 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 60 1 8.092813346 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 90 1 8.929144329 1.372347607 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 180 1 9.51666586 0.166733821 WT 40C 113 137 4 GVSLAASRAAAAEKNVPLYKHLADL 240 1 9.829747803 0.916199561 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 0 1 0 0.046916921 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.337085092 0.379019945 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.468744029 0.281255889 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.610129748 0.241953756 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.56219061 0.332348662 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.652684418 0.648485194 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.746304691 0.452711444 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.753426085 0.30164387 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.861810224 0.386634733 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 30 1 1.85607837 0.271921632 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 60 1 1.987911 0.373370263 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.029076129 0.51805124 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 120 1 2.09368975 0.543597626 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.146665972 0.117415122 Leu343Ala 10C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.387403818 0.426919419 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 0 1 0 0.088184161 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.686901846 0.445833571 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.653900266 0.185948216 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.796501832 0.395968989 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.657374116 0.289116315 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.781737967 0.295994189 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.919476143 0.430849632 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.963594046 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.892727494 0.207810028 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.067288486 0.318592915 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.449064666 0.313188872 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.517673215 0.34438494 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 120 1 2.691539437 0.352491005 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.917339725 0.310732489 Leu343Ala 20C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.908134021 0.263324291 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 0 1 0 0.068533094 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.793375367 0.31490834 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.67821722 0.171209917 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.835756344 0.360105793 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.699234016 0.249568544 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.746130999 0.366001113 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.921039376 0.448289954 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.936845396 0.057479369 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.873447623 0.254726949 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.160735067 0.20609056 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.686328661 0.002702022 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.663227555 0.158191085 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 120 1 2.875479826 0.112011079 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.914734337 0.33406813 Leu343Ala 25C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.971010717 0.194054281 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 0 1 -1.97E-13 0.048390751 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.743699303 0.315890894 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.707223872 0.330137917 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.891511646 0.317610362 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.803102149 0.296731104 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.918433988 0.323260043 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.885258715 0.369685688 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.987042537 0.431095271 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 10 1 2.136418113 0.462536977 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.784638633 0.18103545 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.825282685 0.298450572 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.982300731 0.408496544 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 120 1 3.184305143 0.590514547 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 180 1 3.115696594 0.47383634 Leu343Ala 30C 138 152 2 SKSKTSPYVLPVPFL 240 1 3.041703576 0.457132934 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 0 1 0 0.064848519 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.892553801 0.312451957 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.896722422 0.439201336 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.889601028 0.296976742 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.935282164 0.297713657 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.97279975 0.232865138 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 3 1 2.111232696 0.400144841 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 5 1 2.437600959 0.234830244 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 10 1 2.65332708 0.392038776 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 30 1 3.107011967 0.295257274 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 60 1 3.20879579 0.257428971 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 90 1 3.217827801 0.468677935 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 120 1 3.19298977 0.430603994 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 180 1 3.328469943 0.382458881 Leu343Ala 40C 138 152 2 SKSKTSPYVLPVPFL 240 1 3.425911452 0.397442819 WT 10C 138 152 2 SKSKTSPYVLPVPFL 0 1 -1.97E-13 0.028248408 WT 10C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.229916801 0.068287456 WT 10C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.373734216 0.083025756 WT 10C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.395966859 0.040284686 WT 10C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.464054331 0.166542788 WT 10C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.382592535 0.013018832 WT 10C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.612214059 0.048390751 WT 10C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.548989978 0.237040989 WT 10C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.703576329 0.20609056 WT 10C 138 152 2 SKSKTSPYVLPVPFL 30 1 1.610129748 0.106361397 WT 10C 138 152 2 SKSKTSPYVLPVPFL 60 1 1.837493269 0.056005539 WT 10C 138 152 2 SKSKTSPYVLPVPFL 90 1 1.867020999 0.135101082 WT 10C 138 152 2 SKSKTSPYVLPVPFL 120 1 1.895853959 0.205844921 WT 10C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.141281503 0.122573527 WT 10C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.388619665 0.337261428 WT 20C 138 152 2 SKSKTSPYVLPVPFL 0 1 0 0.03537192 WT 20C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.555242909 0.110537249 WT 20C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.572612162 0.095307672 WT 20C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.712608341 0.259394077 WT 20C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.520851788 WT 20C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.576780783 0.099729162 WT 20C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.609434978 0.021124896 WT 20C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.697497091 0.045443091 WT 20C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.986347767 0.485381342 WT 20C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.188004794 0.49446996 WT 20C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.289962309 0.260867907 WT 20C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.545637712 0.263815567 WT 20C 138 152 2 SKSKTSPYVLPVPFL 120 1 2.6924079 0.205108006 WT 20C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.682507425 0.070743839 WT 20C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.868011047 0.145909169 WT 25C 138 152 2 SKSKTSPYVLPVPFL 0 1 -1.97E-13 0.060181391 WT 25C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.732756674 0.008106065 WT 25C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.592934188 0.090886182 WT 25C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.666406128 0.16334949 WT 25C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.699581401 0.190615345 WT 25C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.748215309 0.077621713 WT 25C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.668664131 0.214687901 WT 25C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.86771577 0.226478541 WT 25C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.851388672 0.007860427 WT 25C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.160561374 0.127240655 WT 25C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.184878328 0.192580451 WT 25C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.678338805 0.022353088 WT 25C 138 152 2 SKSKTSPYVLPVPFL 120 1 2.803223733 0.03684575 WT 25C 138 152 2 SKSKTSPYVLPVPFL 180 1 3.045698505 0.169981725 WT 25C 138 152 2 SKSKTSPYVLPVPFL 240 1 3.322911782 0.578723908 WT 30C 138 152 2 SKSKTSPYVLPVPFL 0 1 -1.97E-13 0.092114374 WT 30C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.620030223 0.134118529 WT 30C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.60682959 0.147874275 WT 30C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.657200424 0.192580451 WT 30C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.670922134 0.277816952 WT 30C 138 152 2 SKSKTSPYVLPVPFL 1 1 1.828113873 0.04716256 WT 30C 138 152 2 SKSKTSPYVLPVPFL 3 1 1.727893283 0.250796736 WT 30C 138 152 2 SKSKTSPYVLPVPFL 5 1 1.742483456 0.242936309 WT 30C 138 152 2 SKSKTSPYVLPVPFL 10 1 1.951261876 0.275360569 WT 30C 138 152 2 SKSKTSPYVLPVPFL 30 1 2.495614264 0.263078652 WT 30C 138 152 2 SKSKTSPYVLPVPFL 60 1 2.751984437 0.083271394 WT 30C 138 152 2 SKSKTSPYVLPVPFL 90 1 2.865405659 0.182754918 WT 30C 138 152 2 SKSKTSPYVLPVPFL 120 1 3.003143835 0.308276105 WT 30C 138 152 2 SKSKTSPYVLPVPFL 180 1 2.999496292 0.093342566 WT 30C 138 152 2 SKSKTSPYVLPVPFL 240 1 2.94513053 0.048390751 WT 40C 138 152 2 SKSKTSPYVLPVPFL 0 1 -1.97E-13 0.005404043 WT 40C 138 152 2 SKSKTSPYVLPVPFL 0.166666667 1 1.782953815 0.219355029 WT 40C 138 152 2 SKSKTSPYVLPVPFL 0.333333333 1 1.938408629 0.147382999 WT 40C 138 152 2 SKSKTSPYVLPVPFL 0.5 1 1.8437462 0.18545694 WT 40C 138 152 2 SKSKTSPYVLPVPFL 0.75 1 1.918433988 0.328909725 WT 40C 138 152 2 SKSKTSPYVLPVPFL 1 1 2.354228545 WT 40C 138 152 2 SKSKTSPYVLPVPFL 3 1 2.166119535 0.119625867 WT 40C 138 152 2 SKSKTSPYVLPVPFL 5 1 2.35666024 0.193071728 WT 40C 138 152 2 SKSKTSPYVLPVPFL 10 1 2.72471471 0.165314597 WT 40C 138 152 2 SKSKTSPYVLPVPFL 30 1 3.0783527 0.209529496 WT 40C 138 152 2 SKSKTSPYVLPVPFL 60 1 3.324996092 0.271430356 WT 40C 138 152 2 SKSKTSPYVLPVPFL 90 1 3.347402428 0.340946003 WT 40C 138 152 2 SKSKTSPYVLPVPFL 180 1 3.409063276 0.116186931 WT 40C 138 152 2 SKSKTSPYVLPVPFL 240 1 3.497646466 0.410952928 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 0 1 0 0.010026853 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 5.906231326 0.252494391 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 6.219268032 0.082645577 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 6.801726858 0.140072099 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 6.952121981 0.124272209 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 1 1 7.369146172 0.974123967 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 3 1 8.360679732 0.422039361 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 5 1 8.368199488 0.684864449 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.663746047 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 30 1 9.633237322 0.161037337 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 60 1 9.656226291 0.104826191 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 90 1 9.735935706 0.438750782 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 120 1 9.945844328 0.459108333 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 180 1 9.819942125 0.237302189 Leu343Ala 10C 153 170 2 NVLNGGSHAGGALALQEF 240 1 10.2120437 0.475212066 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 0 1 2.44E-13 0.023092146 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 7.296741663 0.546919258 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 7.517392793 0.304147876 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 8.347359021 0.518357919 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 8.295150429 0.165594998 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 1 1 8.752996153 0.542665441 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.460282931 0.453942984 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 5 1 8.498828395 1.209603092 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.720466493 0.172887254 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 30 1 9.760858326 0.218463859 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 60 1 10.39810395 0.468223654 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 90 1 10.22557926 0.31721317 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 120 1 10.43140572 0.356105206 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 180 1 10.71672676 0.244290602 Leu343Ala 20C 153 170 2 NVLNGGSHAGGALALQEF 240 1 10.81469844 0.576695973 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 0 1 0 0.038892036 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 7.750934934 0.111206916 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 7.759528941 0.66237999 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 8.652446271 0.071099504 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 8.534923225 0.189294832 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 1 1 8.838721373 0.405631783 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.526886485 0.343951444 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.570501071 0.32177083 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.644624381 0.264344308 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 30 1 9.881604125 0.031903623 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 60 1 10.75303644 0.049526577 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 90 1 10.3452508 0.132172154 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 120 1 10.6415292 0.043145853 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 180 1 10.53260016 0.488885048 Leu343Ala 25C 153 170 2 NVLNGGSHAGGALALQEF 240 1 11.01429425 0.278624978 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 0 1 -2.44E-13 0.036765128 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 8.277532714 0.20722163 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 8.598518877 0.231529153 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 9.256819815 0.173798786 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 9.217717083 0.151922016 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 1 1 9.642046179 0.309009381 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.602943447 0.317517014 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.711442786 0.499519589 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.895784237 0.629564835 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 30 1 10.7023318 0.075961008 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 60 1 10.38499809 0.108472319 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 90 1 10.91804137 0.62774177 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 120 1 11.33721406 0.880236161 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 180 1 11.1399816 0.758698548 Leu343Ala 30C 153 170 2 NVLNGGSHAGGALALQEF 240 1 11.58622542 0.730441053 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 0 1 0 0.013369137 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 9.372624059 0.032815155 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 9.585755433 0.430850838 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 9.641831329 0.03615744 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 9.767303832 0.034942064 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 1 1 9.685875615 0.084468641 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.791152201 0.099053154 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 5 1 10.23804057 0.309920913 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 10 1 10.37232193 0.586722826 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 30 1 11.03663867 0.474300534 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 60 1 11.8674643 0.151314328 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 90 1 12.13452307 0.458500645 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 120 1 12.53736715 0.613157257 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 180 1 12.82934853 0.280448042 Leu343Ala 40C 153 170 2 NVLNGGSHAGGALALQEF 240 1 13.11058741 0.668456871 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 0 1 0 0.013976825 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 6.923546908 0.113333824 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 7.432097273 0.026130587 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 7.428874521 0.500431121 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 8.004028441 0.096622402 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 1 1 7.762107143 0.37980504 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 3 1 8.972573032 0.057122678 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.210412177 0.067149531 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.720681344 0.165291154 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 30 1 9.830684634 0.077784072 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 60 1 10.3383756 0.209956226 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 90 1 10.3974594 0.01579989 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 120 1 10.29712436 0.179571823 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 180 1 10.61725113 0.044057385 WT 10C 153 170 2 NVLNGGSHAGGALALQEF 240 1 10.77559571 0.283182638 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 0 1 0 0.013976825 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 7.982328573 0.186256392 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 8.494746242 0.165898842 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 8.654809623 0.22454074 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 8.930892098 0.271028877 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 1 1 9.057868552 0.196587089 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.143808622 0.587330514 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.460497781 0.578215193 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 10 1 9.861408209 0.202663969 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 30 1 10.6449668 0.54327313 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 60 1 11.1386925 0.809440502 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 90 1 10.92212353 0.171368034 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 120 1 11.47450333 0.599484275 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 180 1 11.18638924 0.16954497 WT 20C 153 170 2 NVLNGGSHAGGALALQEF 240 1 11.75681646 0.171671878 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 0 1 2.44E-13 0.01731911 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 8.829912516 0.060464962 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 8.799188941 0.181091043 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 9.264769271 0.521700203 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 9.306450205 0.274371161 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 1 1 9.914261352 0.574872909 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.632807622 0.974731655 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.832188585 0.058945742 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 10 1 10.41142466 0.078999448 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 30 1 10.84305866 0.11758764 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 60 1 10.7031912 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 90 1 11.74822245 0.735910246 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 120 1 11.78539153 0.207525474 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 180 1 11.53315742 0.553907671 WT 25C 153 170 2 NVLNGGSHAGGALALQEF 240 1 12.50148717 0.887224574 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 0 1 -2.44E-13 0.028865183 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 8.844307477 0.029776715 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 9.075486266 0.068668751 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 9.362740951 0.103610815 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 9.462431433 0.070187971 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 1 1 10.00084597 0.446954571 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 3 1 9.726052598 0.265559684 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 5 1 9.990748014 0.203575502 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 10 1 10.28960461 0.285309546 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 30 1 11.12709059 0.741683283 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 60 1 11.49255074 0.221806143 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 90 1 11.67538824 0.236694501 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 120 1 11.9351421 0.480073571 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 180 1 12.83643859 0.611638037 WT 30C 153 170 2 NVLNGGSHAGGALALQEF 240 1 12.71096609 0.35944749 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 0 1 2.44E-13 0.004253816 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 0.166666667 1 9.692750821 0.107560787 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 0.333333333 1 9.945629477 0.050741953 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 0.5 1 9.986880711 0.09267243 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 0.75 1 10.13040063 0.200233217 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 1 1 10.45675805 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 3 1 10.55730793 0.05955343 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 5 1 10.85788332 0.338786096 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 10 1 11.17306853 0.26069818 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 30 1 12.04170779 0.239732941 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 60 1 12.76231528 0.801236713 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 90 1 13.11767747 0.627437926 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 180 1 13.88146984 0.278928822 WT 40C 153 170 2 NVLNGGSHAGGALALQEF 240 1 14.25316065 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 0 1 0 0.002433798 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.393274716 0.028935152 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.439166849 0.188484123 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 0.5 1 2.652374049 0.378049935 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.352927883 0.077881532 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 1 1 2.601510268 0.573835452 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 3 1 2.861948121 0.407255509 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 5 1 2.547013361 0.193351719 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 10 1 3.370777141 0.399413272 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 30 1 3.953416009 0.434838552 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 60 1 4.511770289 0.204979865 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 90 1 4.955585455 0.335593683 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 120 1 5.40150401 0.415638591 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 180 1 5.944369696 0.184968638 Leu343Ala 10C 170 183 2 FMIAPTGAKTFAEA 240 1 6.318390577 0.269340297 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 0 1 0 0.027583042 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.768442901 0.351007736 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.885850273 0.346680985 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.111869026 0.224450247 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.839575706 0.133588461 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 1 1 3.053930209 0.037588656 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 3 1 3.892035281 0.135751836 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 5 1 3.729691862 0.044619628 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 10 1 4.488824222 0.10627584 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 30 1 5.476843594 0.213092524 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 60 1 6.447079765 0.319097942 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 90 1 6.90542744 0.335052839 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 120 1 7.347712868 0.353711956 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 180 1 7.861513536 0.246895272 Leu343Ala 20C 170 183 2 FMIAPTGAKTFAEA 240 1 7.998233848 0.345599297 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 0 1 -2.17E-13 0.001081688 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.999433302 0.045971737 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.684498541 0.018118273 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.202123554 0.167120787 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.929065365 0.125475801 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 1 1 2.946083697 0.223638981 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 3 1 3.818225434 0.364528836 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 5 1 3.83983298 0.115470188 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 10 1 4.392450744 0.162523613 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 30 1 5.813003466 0.093025163 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 60 1 6.979237286 0.371289385 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 90 1 7.280213189 0.033261904 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 120 1 7.711216802 0.03542528 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 180 1 7.91486314 0.224450247 Leu343Ala 25C 170 183 2 FMIAPTGAKTFAEA 240 1 8.340130236 0.004326752 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 0 1 2.17E-13 0.023797135 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.686984365 0.166039099 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.737656928 0.061385791 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.341138472 0.028935152 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 0.75 1 3.037294311 0.10221951 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 1 1 3.295055123 0.350466892 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 3 1 3.859910788 0.22066434 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 5 1 4.470276152 0.405632977 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 10 1 5.331709725 0.295030385 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 30 1 7.283272665 0.070580138 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 60 1 7.684637608 0.226613623 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 90 1 8.178742903 0.487300417 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 120 1 8.739583007 0.78341249 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 180 1 8.673039415 0.730409781 Leu343Ala 30C 170 183 2 FMIAPTGAKTFAEA 240 1 8.853357252 0.532190466 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 0 1 -2.17E-13 0.023526713 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 3.187208611 0.226884045 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 3.303277463 0.009194347 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.457016107 0.445114587 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 0.75 1 3.843848542 0.444844165 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 1 1 3.962785652 0.394545676 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 3 1 5.092305767 0.038940766 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 5 1 5.92754258 0.115199766 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 10 1 6.906001091 0.269069875 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 30 1 8.286589415 0.434027286 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 60 1 8.938448915 0.32017963 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 90 1 9.170777836 0.524348229 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 120 1 9.276903393 0.42239914 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 180 1 9.183206956 0.296382495 Leu343Ala 40C 170 183 2 FMIAPTGAKTFAEA 240 1 9.422037263 0.581677689 WT 10C 170 183 2 FMIAPTGAKTFAEA 0 1 0 0.012709833 WT 10C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.50991722 0.063008322 WT 10C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.473585949 0.024067557 WT 10C 170 183 2 FMIAPTGAKTFAEA 0.5 1 2.741863707 0.191999609 WT 10C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.721785899 0.090591365 WT 10C 170 183 2 FMIAPTGAKTFAEA 1 1 2.470144039 0.015414053 WT 10C 170 183 2 FMIAPTGAKTFAEA 3 1 3.027159798 0.210929148 WT 10C 170 183 2 FMIAPTGAKTFAEA 5 1 2.936714054 0.07220267 WT 10C 170 183 2 FMIAPTGAKTFAEA 10 1 3.382632609 0.263120591 WT 10C 170 183 2 FMIAPTGAKTFAEA 30 1 4.129144633 0.20579113 WT 10C 170 183 2 FMIAPTGAKTFAEA 60 1 5.135712075 0.134399726 WT 10C 170 183 2 FMIAPTGAKTFAEA 90 1 5.603620611 0.180101042 WT 10C 170 183 2 FMIAPTGAKTFAEA 120 1 5.877634886 0.368585165 WT 10C 170 183 2 FMIAPTGAKTFAEA 180 1 6.567164179 0.118715251 WT 10C 170 183 2 FMIAPTGAKTFAEA 240 1 6.844620364 0.242298098 WT 20C 170 183 2 FMIAPTGAKTFAEA 0 1 0 0.017577429 WT 20C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.579329071 0.312066971 WT 20C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.851813608 0.033802748 WT 20C 170 183 2 FMIAPTGAKTFAEA 0.5 1 2.79253627 0.458906108 WT 20C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.829823628 WT 20C 170 183 2 FMIAPTGAKTFAEA 1 1 2.614895474 0.083289971 WT 20C 170 183 2 FMIAPTGAKTFAEA 3 1 3.419155098 0.511638395 WT 20C 170 183 2 FMIAPTGAKTFAEA 5 1 3.612093272 0.64171137 WT 20C 170 183 2 FMIAPTGAKTFAEA 10 1 4.487294485 0.143323652 WT 20C 170 183 2 FMIAPTGAKTFAEA 30 1 5.778201932 0.276100847 WT 20C 170 183 2 FMIAPTGAKTFAEA 60 1 6.703693273 0.232292485 WT 20C 170 183 2 FMIAPTGAKTFAEA 90 1 7.323619498 0.202005223 WT 20C 170 183 2 FMIAPTGAKTFAEA 120 1 7.448293125 0.037047812 WT 20C 170 183 2 FMIAPTGAKTFAEA 180 1 7.589793867 0.187943279 WT 20C 170 183 2 FMIAPTGAKTFAEA 240 1 8.0565551 0.006490128 WT 25C 170 183 2 FMIAPTGAKTFAEA 0 1 -2.17E-13 0.012980255 WT 25C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 3.101352079 0.378861201 WT 25C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.748365093 0.090591365 WT 25C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.284155741 0.541114392 WT 25C 170 183 2 FMIAPTGAKTFAEA 0.75 1 2.990446092 0.407525931 WT 25C 170 183 2 FMIAPTGAKTFAEA 1 1 3.433687607 0.662804285 WT 25C 170 183 2 FMIAPTGAKTFAEA 3 1 3.956666701 0.74798721 WT 25C 170 183 2 FMIAPTGAKTFAEA 5 1 3.855512792 0.366421789 WT 25C 170 183 2 FMIAPTGAKTFAEA 10 1 5.09001116 0.290703634 WT 25C 170 183 2 FMIAPTGAKTFAEA 30 1 6.521272046 0.314230346 WT 25C 170 183 2 FMIAPTGAKTFAEA 60 1 7.197033699 WT 25C 170 183 2 FMIAPTGAKTFAEA 90 1 7.649262423 0.106816684 WT 25C 170 183 2 FMIAPTGAKTFAEA 120 1 7.886180557 0.136833524 WT 25C 170 183 2 FMIAPTGAKTFAEA 180 1 8.466907252 0.330726087 WT 25C 170 183 2 FMIAPTGAKTFAEA 240 1 8.354471527 0.135481414 WT 30C 170 183 2 FMIAPTGAKTFAEA 0 1 0 0.003245064 WT 30C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 2.91223825 0.347221829 WT 30C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 2.825043198 0.061115369 WT 30C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.073243148 0.081667439 WT 30C 170 183 2 FMIAPTGAKTFAEA 0.75 1 3.181280877 0.25879384 WT 30C 170 183 2 FMIAPTGAKTFAEA 1 1 3.410932757 0.144946184 WT 30C 170 183 2 FMIAPTGAKTFAEA 3 1 4.073309205 0.038940766 WT 30C 170 183 2 FMIAPTGAKTFAEA 5 1 4.762073629 0.259064261 WT 30C 170 183 2 FMIAPTGAKTFAEA 10 1 5.666148642 0.185509482 WT 30C 170 183 2 FMIAPTGAKTFAEA 30 1 7.199328306 0.137915212 WT 30C 170 183 2 FMIAPTGAKTFAEA 60 1 7.891152205 0.071391404 WT 30C 170 183 2 FMIAPTGAKTFAEA 90 1 8.077780211 0.182805262 WT 30C 170 183 2 FMIAPTGAKTFAEA 120 1 8.284103591 0.281509286 WT 30C 170 183 2 FMIAPTGAKTFAEA 180 1 8.701721998 0.176044712 WT 30C 170 183 2 FMIAPTGAKTFAEA 240 1 8.789873136 0.164957411 WT 40C 170 183 2 FMIAPTGAKTFAEA 0 1 2.17E-13 0.003245064 WT 40C 170 183 2 FMIAPTGAKTFAEA 0.166666667 1 3.105176424 0.178748932 WT 40C 170 183 2 FMIAPTGAKTFAEA 0.333333333 1 3.440571426 0.550308739 WT 40C 170 183 2 FMIAPTGAKTFAEA 0.5 1 3.556066627 0.329103556 WT 40C 170 183 2 FMIAPTGAKTFAEA 0.75 1 3.769082609 0.18523906 WT 40C 170 183 2 FMIAPTGAKTFAEA 1 1 4.127614895 WT 40C 170 183 2 FMIAPTGAKTFAEA 3 1 5.700567741 0.21796012 WT 40C 170 183 2 FMIAPTGAKTFAEA 5 1 6.429678998 0.072743514 WT 40C 170 183 2 FMIAPTGAKTFAEA 10 1 7.32763506 0.002974642 WT 40C 170 183 2 FMIAPTGAKTFAEA 30 1 8.346440404 0.172799648 WT 40C 170 183 2 FMIAPTGAKTFAEA 60 1 8.689484096 0.441599101 WT 40C 170 183 2 FMIAPTGAKTFAEA 90 1 9.017421627 0.432675176 WT 40C 170 183 2 FMIAPTGAKTFAEA 180 1 9.434466382 0.190106655 WT 40C 170 183 2 FMIAPTGAKTFAEA 240 1 9.697390058 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 0 0.037143874 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 10.29997769 0.954464904 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 10.45334469 0.490829763 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 11.71545661 0.105462071 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 11.11418293 0.525983787 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 12.04095111 2.555631185 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 13.90433671 1.883725036 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 13.45689904 1.615758517 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 16.21985012 1.031405786 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 18.73844582 0.173116984 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 20.2289667 0.768745534 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 21.21905153 1.269524549 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 21.8850489 1.412793777 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 22.09704243 0.468278125 Leu343Ala 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 23.07399492 0.873544321 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 5.33E-13 0.045766559 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 12.18118576 1.567338824 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 12.45743397 0.490829763 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 13.67967985 0.766755684 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 13.27632933 0.577056613 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 14.24390157 0.849666117 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 17.096903 0.64736466 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 17.95988551 0.679202267 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 19.26514656 0.598944967 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 21.31332299 0.5094017 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 22.86716052 0.478227377 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 23.39104719 0.319702629 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 24.06642481 0.504095432 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 25.02039569 0.030511039 Leu343Ala 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 25.29992698 0.632772424 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 -5.33E-13 0.045766559 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 13.01086841 0.368785606 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 12.52637877 0.602924668 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 14.16698357 0.073624464 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 13.82038352 0.193678771 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 14.41790511 0.582362881 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 17.40598206 0.190362354 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 18.08511178 0.880177156 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 19.55640316 0.149902063 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 22.05952145 0.565780794 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 24.0317179 1.1912571 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 24.04016012 0.620833322 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 24.87265684 0.640068542 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 25.109039 0.185056086 Leu343Ala 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 25.98796793 0.580373031 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 0 0.004642984 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 13.24302946 0.426491267 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 14.17167369 0.878850589 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 16.21797407 1.22574784 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 15.84698539 0.930586699 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 17.13864509 1.47845884 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 19.22105941 1.2754941 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 20.40297024 1.573971659 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 21.95727679 1.613768667 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 24.68974207 0.307100244 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 24.86046252 0.090869834 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 26.39178747 1.065233242 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 27.80679738 1.993166808 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 27.04418349 0.827114479 Leu343Ala 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 27.94890809 1.391568706 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 5.33E-13 0.050409543 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 16.23204444 0.060358795 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 17.07157634 1.24100336 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 17.83231418 0.309753377 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 19.50528082 0.236128913 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 19.54092575 0.143269228 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 21.69416092 0.998241612 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 23.06836677 0.877524022 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 24.25309168 1.255595596 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 26.88847142 1.089111447 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 28.84612849 0.130003559 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 29.2987253 1.152123376 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 29.82308098 0.812522243 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 29.49993155 0.295824425 Leu343Ala 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 30.30053543 1.226411124 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 0 0.098829236 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 11.29756671 0.760786133 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 11.95746693 0.49215633 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 12.65113602 0.108115205 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 13.64403492 1.126255321 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 13.23505626 0.569097212 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 16.05241275 0.667926448 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 17.12598176 1.010843998 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 18.56491129 0.40592948 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 19.97100997 0.466951558 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 22.05576936 0.252711 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 22.63453045 0.754153298 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 23.13215244 1.00155803 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 24.56686086 0.279242338 WT 10C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 24.82669364 0.720989125 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 -5.33E-13 0.009285968 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 13.03291198 0.06898148 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 14.54922854 0.324345614 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 14.99666621 0.365469188 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 15.10594606 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 15.93750475 0.30444711 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 17.8407564 1.105693534 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 18.58507882 1.067886376 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 20.06621946 0.054389244 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 22.92438002 1.00155803 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 24.46789928 0.720989125 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 25.52599088 0.346897251 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 25.95466806 0.012602386 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 26.32331168 0.506085283 WT 20C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 27.46770153 0.315059645 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 0 0.007296118 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 15.92859352 0.474247676 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 15.38172525 0.113421472 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 17.05140881 0.491493046 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 17.15693656 1.391568706 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 18.6573067 0.843033282 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 19.57985377 1.560042706 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 19.97710713 0.357509787 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 21.85174903 0.171127134 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 24.23339316 0.318376062 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 25.47908966 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 27.10140298 0.202301456 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 27.51038165 0.308426811 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 27.81242553 0.215567126 WT 25C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 28.53564239 0.705070322 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 0 0.026531339 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 15.31653255 0.116074606 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 16.11901249 0.728285243 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 17.29435715 0.344907401 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 17.94534613 0.241435181 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 19.32564914 0.207607724 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 19.92786085 0.491493046 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 20.96015678 0.834410597 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 22.69690908 0.913341329 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 25.52552187 0.959107888 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 27.3743681 0.143269228 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 27.71205691 0.565117511 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 120 1 28.31473763 0.673232715 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 29.21336507 0.401286495 WT 30C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 29.51869204 0.534606472 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0 1 5.33E-13 0.044439992 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.166666667 1 17.96692069 0.629456007 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.333333333 1 19.08035574 0.033827457 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.5 1 20.00149577 0.352203519 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 0.75 1 20.44424332 0.652007645 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 1 1 21.55814737 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 3 1 23.43701039 0.314396362 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 5 1 24.95614102 0.982322809 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 10 1 26.4466619 0.439756936 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 30 1 28.67681508 0.700427337 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 60 1 29.30857456 1.274167533 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 90 1 30.4182575 1.376313187 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 180 1 31.64425549 0.418531865 WT 40C 184 223 5 LRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPNIQTAE 240 1 31.71601436 Leu343Ala 10C 224 228 1 EALDL 0 1 -1.00E-13 0.033975773 Leu343Ala 10C 224 228 1 EALDL 0.166666667 1 0.213404439 0.100558333 Leu343Ala 10C 224 228 1 EALDL 0.333333333 1 0.223612651 0.042563057 Leu343Ala 10C 224 228 1 EALDL 0.5 1 0.281165848 0.009956271 Leu343Ala 10C 224 228 1 EALDL 0.75 1 0.184363835 0.094086757 Leu343Ala 10C 224 228 1 EALDL 1 1 0.426192865 Leu343Ala 10C 224 228 1 EALDL 3 1 0.2644455 0.164029558 Leu343Ala 10C 224 228 1 EALDL 5 1 0.27834979 Leu343Ala 10C 224 228 1 EALDL 10 1 0.391784149 0.052394874 Leu343Ala 10C 224 228 1 EALDL 30 1 0.298238203 0.10080724 Leu343Ala 10C 224 228 1 EALDL 60 1 0.252213246 0.103669667 Leu343Ala 10C 224 228 1 EALDL 90 1 0.211204393 0.153948834 Leu343Ala 10C 224 228 1 EALDL 120 1 0.256877343 0.229492037 Leu343Ala 10C 224 228 1 EALDL 180 1 0.261893447 0.149219605 Leu343Ala 10C 224 228 1 EALDL 240 1 0.315574564 0.036215934 Leu343Ala 20C 224 228 1 EALDL 0 1 -1.00E-13 0.004231415 Leu343Ala 20C 224 228 1 EALDL 0.166666667 1 0.277557773 0.326565675 Leu343Ala 20C 224 228 1 EALDL 0.333333333 1 0.312406498 0.008960644 Leu343Ala 20C 224 228 1 EALDL 0.5 1 0.338895049 0.148223978 Leu343Ala 20C 224 228 1 EALDL 0.75 1 0.225900699 0.137520987 Leu343Ala 20C 224 228 1 EALDL 1 1 0.279845821 0.04306087 Leu343Ala 20C 224 228 1 EALDL 3 1 0.454705458 0.069071627 Leu343Ala 20C 224 228 1 EALDL 5 1 0.413872609 0.082512592 Leu343Ala 20C 224 228 1 EALDL 10 1 0.32789482 0.107278815 Leu343Ala 20C 224 228 1 EALDL 30 1 0.371367724 0.022152702 Leu343Ala 20C 224 228 1 EALDL 60 1 0.268581586 0.159051422 Leu343Ala 20C 224 228 1 EALDL 90 1 0.321734692 0.040073989 Leu343Ala 20C 224 228 1 EALDL 120 1 0.383511977 0.066209199 Leu343Ala 20C 224 228 1 EALDL 180 1 0.397768274 0.021903795 Leu343Ala 20C 224 228 1 EALDL 240 1 0.3653836 0.08537502 Leu343Ala 25C 224 228 1 EALDL 0 1 -1.00E-13 0.001742347 Leu343Ala 25C 224 228 1 EALDL 0.166666667 1 0.460689582 0.302048358 Leu343Ala 25C 224 228 1 EALDL 0.333333333 1 0.274741715 0.078654537 Leu343Ala 25C 224 228 1 EALDL 0.5 1 0.374447789 0.090975422 Leu343Ala 25C 224 228 1 EALDL 0.75 1 0.247989158 0.11847962 Leu343Ala 25C 224 228 1 EALDL 1 1 0.288205995 0.030491079 Leu343Ala 25C 224 228 1 EALDL 3 1 0.357815443 0.161042676 Leu343Ala 25C 224 228 1 EALDL 5 1 0.263213475 0.135280826 Leu343Ala 25C 224 228 1 EALDL 10 1 0.223876657 0.05077698 Leu343Ala 25C 224 228 1 EALDL 30 1 0.287941989 0.12843589 Leu343Ala 25C 224 228 1 EALDL 60 1 0.249573191 0.163033931 Leu343Ala 25C 224 228 1 EALDL 90 1 0.24376507 0.120221967 Leu343Ala 25C 224 228 1 EALDL 120 1 0.454617456 0.0846283 Leu343Ala 25C 224 228 1 EALDL 180 1 0.361687523 0.105785375 Leu343Ala 25C 224 228 1 EALDL 240 1 0.346991217 0.013814325 Leu343Ala 30C 224 228 1 EALDL 0 1 0 0.040198442 Leu343Ala 30C 224 228 1 EALDL 0.166666667 1 0.179963743 0.117483993 Leu343Ala 30C 224 228 1 EALDL 0.333333333 1 0.235844906 0.087988541 Leu343Ala 30C 224 228 1 EALDL 0.5 1 0.428744918 0.043932044 Leu343Ala 30C 224 228 1 EALDL 0.75 1 0.184011827 0.09234441 Leu343Ala 30C 224 228 1 EALDL 1 1 0.290054033 0.223642728 Leu343Ala 30C 224 228 1 EALDL 3 1 0.228804759 0.174359188 Leu343Ala 30C 224 228 1 EALDL 5 1 0.271045638 0.195516263 Leu343Ala 30C 224 228 1 EALDL 10 1 0.245965116 0.072431868 Leu343Ala 30C 224 228 1 EALDL 30 1 0.282749881 Leu343Ala 30C 224 228 1 EALDL 60 1 0.152067163 0.088984168 Leu343Ala 30C 224 228 1 EALDL 90 1 0.308094408 0.011449711 Leu343Ala 30C 224 228 1 EALDL 120 1 0.199148142 0.164527371 Leu343Ala 30C 224 228 1 EALDL 180 1 0.316982593 0.078281177 Leu343Ala 30C 224 228 1 EALDL 240 1 0.191491983 0.108398896 Leu343Ala 40C 224 228 1 EALDL 0 1 0 0.023646143 Leu343Ala 40C 224 228 1 EALDL 0.166666667 1 0.363359558 0.083259313 Leu343Ala 40C 224 228 1 EALDL 0.333333333 1 0.373831776 0.102798494 Leu343Ala 40C 224 228 1 EALDL 0.5 1 0.313286516 0.042563057 Leu343Ala 40C 224 228 1 EALDL 0.75 1 0.34039108 0.004231415 Leu343Ala 40C 224 228 1 EALDL 1 1 0.316102575 0.055755115 Leu343Ala 40C 224 228 1 EALDL 3 1 Leu343Ala 40C 224 228 1 EALDL 5 1 0.313638524 0.106034282 Leu343Ala 40C 224 228 1 EALDL 10 1 0.394336202 0.055630662 Leu343Ala 40C 224 228 1 EALDL 30 1 0.364855589 0.130427144 Leu343Ala 40C 224 228 1 EALDL 60 1 0.363359558 0.038705002 Leu343Ala 40C 224 228 1 EALDL 90 1 0.262949469 0.084130486 Leu343Ala 40C 224 228 1 EALDL 120 1 0.309414436 0.201863386 Leu343Ala 40C 224 228 1 EALDL 180 1 Leu343Ala 40C 224 228 1 EALDL 240 1 0.272893676 0.067578186 WT 20C 224 228 1 EALDL 0 1 1.00E-13 0.021032622 WT 20C 224 228 1 EALDL 0.166666667 1 0.102786138 0.153202113 WT 20C 224 228 1 EALDL 0.333333333 1 0.145379024 0.030739985 WT 20C 224 228 1 EALDL 0.5 1 0.133498777 0.165025185 WT 20C 224 228 1 EALDL 0.75 1 0.117130436 WT 20C 224 228 1 EALDL 1 1 0.055705159 0.092593316 WT 20C 224 228 1 EALDL 3 1 0.086329796 WT 20C 224 228 1 EALDL 5 1 0.180315751 0.180955218 WT 20C 224 228 1 EALDL 10 1 0.171339564 0.089108622 WT 20C 224 228 1 EALDL 30 1 0.0817537 0.037584921 WT 20C 224 228 1 EALDL 60 1 0.151363148 0.165149638 WT 20C 224 228 1 EALDL 90 1 0.29278209 0.147103898 WT 20C 224 228 1 EALDL 120 1 0.147755073 0.088361901 WT 20C 224 228 1 EALDL 180 1 0.105690198 0.071685148 WT 20C 224 228 1 EALDL 240 1 0.199852157 0.197880878 WT 25C 224 228 1 EALDL 0 1 1.00E-13 0.026633024 WT 25C 224 228 1 EALDL 0.166666667 1 0.29375011 0.133662932 WT 25C 224 228 1 EALDL 0.333333333 1 0.137546861 0.088237448 WT 25C 224 228 1 EALDL 0.5 1 0.360983508 0.089855342 WT 25C 224 228 1 EALDL 0.75 1 0.17934773 0.113999298 WT 25C 224 228 1 EALDL 1 1 0.314870549 0.165274091 WT 25C 224 228 1 EALDL 3 1 0.242357041 WT 25C 224 228 1 EALDL 5 1 0.118714469 0.100682786 WT 25C 224 228 1 EALDL 10 1 0.257053347 0.095704651 WT 25C 224 228 1 EALDL 30 1 0.299382227 WT 25C 224 228 1 EALDL 60 1 0.207508316 0.003733601 WT 25C 224 228 1 EALDL 90 1 0.375063801 0.164029558 WT 25C 224 228 1 EALDL 120 1 0.340567084 0.052519327 WT 25C 224 228 1 EALDL 180 1 0.23030079 0.237083693 WT 25C 224 228 1 EALDL 240 1 0.251069222 0.275166428 WT 30C 224 228 1 EALDL 0 1 1.00E-13 0.040198442 WT 30C 224 228 1 EALDL 0.166666667 1 0.207068307 0.121466501 WT 30C 224 228 1 EALDL 0.333333333 1 0.121970537 0.012818698 WT 30C 224 228 1 EALDL 0.5 1 0.134818804 0.089730889 WT 30C 224 228 1 EALDL 0.75 1 0.18559586 0.112754764 WT 30C 224 228 1 EALDL 1 1 0.193956034 0.063097865 WT 30C 224 228 1 EALDL 3 1 0.165531443 0.070191708 WT 30C 224 228 1 EALDL 5 1 0.198180122 0.098691532 WT 30C 224 228 1 EALDL 10 1 0.115106394 0.042936417 WT 30C 224 228 1 EALDL 30 1 0.221764613 0.265459064 WT 30C 224 228 1 EALDL 60 1 0.134554799 0.00074672 WT 30C 224 228 1 EALDL 90 1 0.182867804 0.024766223 WT 30C 224 228 1 EALDL 120 1 0.175123643 0.01804574 WT 30C 224 228 1 EALDL 180 1 0.113786367 0.125449009 WT 30C 224 228 1 EALDL 240 1 0.205220269 0.201738932 WT 40C 224 228 1 EALDL 0 1 1.00E-13 0.010827444 WT 40C 224 228 1 EALDL 0.166666667 1 0.19184399 0.000871174 WT 40C 224 228 1 EALDL 0.333333333 1 0.250629213 0.278900029 WT 40C 224 228 1 EALDL 0.5 1 0.198444128 0.00771611 WT 40C 224 228 1 EALDL 0.75 1 0.092665927 0.126444636 WT 40C 224 228 1 EALDL 1 1 0.178731718 WT 40C 224 228 1 EALDL 3 1 0.387824067 0.148597338 WT 40C 224 228 1 EALDL 5 1 0.355263389 0.006720483 WT 40C 224 228 1 EALDL 10 1 0.200204164 0.070689521 WT 40C 224 228 1 EALDL 30 1 0.324638752 0.127938077 WT 40C 224 228 1 EALDL 60 1 0.147931077 WT 40C 224 228 1 EALDL 90 1 0.319622648 0.205970347 WT 40C 224 228 1 EALDL 180 1 0.056321171 0.014561046 WT 40C 224 228 1 EALDL 240 1 0.28459792 0.293834435 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.060220106 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 3.328993762 0.113919563 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 3.191483591 0.596063975 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 3.375372932 0.490199331 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 3.101979929 0.11123459 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 3.345538378 1.666984579 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 4.074857608 0.734531861 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 3.727149444 0.348662904 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 5.250339029 1.190977247 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 5.38025495 1.026426768 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 5.787903445 0.598748948 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 5.544887442 0.403896632 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 5.932465419 0.090521942 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 6.18714402 0.120823779 Leu343Ala 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 6.837808516 0.398143118 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.067507889 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 4.126118796 0.255839557 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 4.075671277 0.898698773 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 4.52617304 0.529323221 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 4.061838893 0.137317183 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 4.686465961 0.000383568 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 5.754000542 0.312607554 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 5.615676702 0.346361499 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 6.437754272 0.70959997 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 6.463249254 0.038356755 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.542446433 0.203290802 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 6.83048549 0.188715235 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 7.376457825 0.825053803 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 7.784648766 0.121974481 Leu343Ala 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 7.675617033 0.3321695 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 3.08E-13 0.056000863 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 3.885272579 0.921712826 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 3.988608625 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 4.922701383 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 3.966639544 0.628667217 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 4.224572823 1.549612908 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 5.013561161 0.265045178 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 5.427990236 0.064822916 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 5.859506374 0.859191315 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 6.096284242 0.28038788 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.326552753 0.082083456 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 6.589096827 0.322196743 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 7.813940873 0.612173812 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 7.039056143 0.066357186 Leu343Ala 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 7.871711418 0.662421161 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.008054919 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 3.682397613 0 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 3.834282615 0.41195155 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 5.361811771 0.053699457 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 4.113371305 0.314141825 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 4.959587741 0.367074147 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 5.625440738 0.74718959 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 6.02441009 0.137700751 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 6.248440467 0.140002156 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 7.685923515 1.826165113 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.632221318 0.417321496 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 7.410903173 0.097809726 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 7.147274207 1.088948279 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 7.844589097 0.021479783 Leu343Ala 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 7.639001899 0.116604536 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 -3.08E-13 0.098576861 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 5.079468402 0.220551342 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 5.433143477 0.199838694 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 5.544616219 1.200566436 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 5.862489829 0.589159759 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 6.131000814 0.625982244 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 6.160835368 0.754093806 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 6.693788988 0.446089062 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 6.91483591 0.141919994 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 7.703010578 0.687353052 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 8.243287225 0.385101822 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 8.311635476 0.087453402 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 8.693788988 0.105481077 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 9.386764307 0.489432196 Leu343Ala 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 9.475725522 0.240880422 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.079014916 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 3.374559262 0.169536858 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 3.388120423 0.236277612 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 4.080553295 0.209044316 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 4.103607269 0.121207346 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 3.58096013 0.081699888 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 4.627610523 0.339840851 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 4.485760781 0.192167343 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 5.416598861 0.449157603 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 5.857879034 0.419622901 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.514239219 0.125043022 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 6.474911852 0.037973188 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 6.400054245 0.143837832 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 7.566856523 0.597981813 WT 10C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 6.894765392 0.285757826 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.166468317 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 3.560618389 0.648996297 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 4.019256848 0.062521511 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 4.051261188 0.831958019 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 3.779766748 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 3.738269596 0.23972972 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 4.960943857 1.13267498 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 4.960943857 1.39580232 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 5.787903445 0.116604536 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 6.353132628 0.053699457 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.927854624 0.580337705 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 7.805804177 0.316059662 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 6.5969623 0.831958019 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 6.848928668 0.416554361 WT 20C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 7.295362083 0.613708082 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 -3.08E-13 0.029534701 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 5.001356116 0.435349171 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 4.132899376 0.050247349 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 5.099538921 0.964672392 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 4.310008137 0.846917154 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 5.230810957 1.238539624 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 6.237862761 1.52583172 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 5.164632493 0.118522373 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 6.524274478 0.550803004 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 7.87523732 1.301444702 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 7.204502305 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 8.077841063 1.217443408 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 7.830214266 0.358635661 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 6.918090589 0.582255543 WT 25C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 8.270138324 0.542748085 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 3.08E-13 0.026466161 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 4.436940602 0.353649282 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 4.018985625 0.150742048 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 4.176837537 0.489815763 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 4.779495525 0.700010781 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 4.956875509 0.65321554 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 5.150800108 0.240113287 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 6.02359642 0.242414693 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 6.162191484 0.334854472 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 7.208841877 0.97119304 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 6.947111473 0.147673507 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 7.225386493 0.553487977 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 120 1 7.412530513 0.396225281 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 7.822620016 0.532008194 WT 30C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 8.10794684 0.574200625 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0 1 0 0.107782482 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.166666667 1 5.018443179 0.056000863 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.333333333 1 5.432601031 0.904835854 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.5 1 5.412801736 0.866095531 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 0.75 1 5.153783564 0.245099665 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 1 1 5.986710062 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 3 1 7.442907513 0.420773604 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 5 1 7.393816111 0.181043884 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 10 1 7.575264443 0.413869388 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 30 1 8.170056957 0.563077166 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 60 1 9.244372118 1.348239944 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 90 1 9.370219691 0.617927325 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 180 1 9.155682126 0.306086906 WT 40C 229 253 3 IVDAIKAAGHDGKIKIGLDCASSEF 240 1 10.06400868 0.695407971 Leu343Ala 10C 253 262 2 FFKDGKYDLD 0 1 0 0.194755696 Leu343Ala 10C 253 262 2 FFKDGKYDLD 0.166666667 1 1.620483229 0.081718194 Leu343Ala 10C 253 262 2 FFKDGKYDLD 0.333333333 1 1.704231105 0.117357407 Leu343Ala 10C 253 262 2 FFKDGKYDLD 0.5 1 1.928746689 0.141836866 Leu343Ala 10C 253 262 2 FFKDGKYDLD 0.75 1 1.668593711 0.053278823 Leu343Ala 10C 253 262 2 FFKDGKYDLD 1 1 2.030313263 0.828341696 Leu343Ala 10C 253 262 2 FFKDGKYDLD 3 1 2.212063974 0.535308171 Leu343Ala 10C 253 262 2 FFKDGKYDLD 5 1 1.873254175 0.361432013 Leu343Ala 10C 253 262 2 FFKDGKYDLD 10 1 2.807717564 0.191155776 Leu343Ala 10C 253 262 2 FFKDGKYDLD 30 1 2.839027561 0.388071425 Leu343Ala 10C 253 262 2 FFKDGKYDLD 60 1 3.122599398 0.286553668 Leu343Ala 10C 253 262 2 FFKDGKYDLD 90 1 3.170455327 0.302393318 Leu343Ala 10C 253 262 2 FFKDGKYDLD 120 1 3.557630161 0.312113103 Leu343Ala 10C 253 262 2 FFKDGKYDLD 180 1 3.581303573 0.084958123 Leu343Ala 10C 253 262 2 FFKDGKYDLD 240 1 3.939204833 0.048958918 Leu343Ala 20C 253 262 2 FFKDGKYDLD 0 1 0 0.123837264 Leu343Ala 20C 253 262 2 FFKDGKYDLD 0.166666667 1 2.672804571 0.163436389 Leu343Ala 20C 253 262 2 FFKDGKYDLD 0.333333333 1 2.587020272 0.536748139 Leu343Ala 20C 253 262 2 FFKDGKYDLD 0.5 1 2.899102025 0.03347926 Leu343Ala 20C 253 262 2 FFKDGKYDLD 0.75 1 2.83622748 0.409670947 Leu343Ala 20C 253 262 2 FFKDGKYDLD 1 1 3.031724042 0.276473891 Leu343Ala 20C 253 262 2 FFKDGKYDLD 3 1 3.416098795 0.109437582 Leu343Ala 20C 253 262 2 FFKDGKYDLD 5 1 3.435444809 0.546467925 Leu343Ala 20C 253 262 2 FFKDGKYDLD 10 1 3.591994791 0.194755696 Leu343Ala 20C 253 262 2 FFKDGKYDLD 30 1 4.031607505 0.190795784 Leu343Ala 20C 253 262 2 FFKDGKYDLD 60 1 4.237286181 0.144716802 Leu343Ala 20C 253 262 2 FFKDGKYDLD 90 1 4.487002493 0.278993835 Leu343Ala 20C 253 262 2 FFKDGKYDLD 120 1 4.581441588 0.381591568 Leu343Ala 20C 253 262 2 FFKDGKYDLD 180 1 4.815630179 0.154076595 Leu343Ala 20C 253 262 2 FFKDGKYDLD 240 1 4.949270408 0.155876556 Leu343Ala 25C 253 262 2 FFKDGKYDLD 0 1 -2.89E-13 0.161276436 Leu343Ala 25C 253 262 2 FFKDGKYDLD 0.166666667 1 2.8064448 0.062998608 Leu343Ala 25C 253 262 2 FFKDGKYDLD 0.333333333 1 2.489781097 0.436670351 Leu343Ala 25C 253 262 2 FFKDGKYDLD 0.5 1 2.981068032 0.186835871 Leu343Ala 25C 253 262 2 FFKDGKYDLD 0.75 1 2.829100001 0.437030343 Leu343Ala 25C 253 262 2 FFKDGKYDLD 1 1 2.851246096 0.058678703 Leu343Ala 25C 253 262 2 FFKDGKYDLD 3 1 3.361115387 0.069118473 Leu343Ala 25C 253 262 2 FFKDGKYDLD 5 1 3.256494179 0.33083269 Leu343Ala 25C 253 262 2 FFKDGKYDLD 10 1 3.515883499 0.124557248 Leu343Ala 25C 253 262 2 FFKDGKYDLD 30 1 4.114082618 0.344872379 Leu343Ala 25C 253 262 2 FFKDGKYDLD 60 1 4.364308036 0.361792005 Leu343Ala 25C 253 262 2 FFKDGKYDLD 90 1 4.367108117 0.146876754 Leu343Ala 25C 253 262 2 FFKDGKYDLD 120 1 4.694972144 0.579587193 Leu343Ala 25C 253 262 2 FFKDGKYDLD 180 1 4.860431475 0.254874368 Leu343Ala 25C 253 262 2 FFKDGKYDLD 240 1 5.092329091 0.395631258 Leu343Ala 30C 253 262 2 FFKDGKYDLD 0 1 0 0.193315728 Leu343Ala 30C 253 262 2 FFKDGKYDLD 0.166666667 1 2.52440028 0.515508609 Leu343Ala 30C 253 262 2 FFKDGKYDLD 0.333333333 1 2.676877417 0.332992642 Leu343Ala 30C 253 262 2 FFKDGKYDLD 0.5 1 3.126926796 0.168836269 Leu343Ala 30C 253 262 2 FFKDGKYDLD 0.75 1 2.847427803 0.41723078 Leu343Ala 30C 253 262 2 FFKDGKYDLD 1 1 3.055142902 0.116997415 Leu343Ala 30C 253 262 2 FFKDGKYDLD 3 1 3.38402514 0.309233167 Leu343Ala 30C 253 262 2 FFKDGKYDLD 5 1 3.497810249 0.033839252 Leu343Ala 30C 253 262 2 FFKDGKYDLD 10 1 3.855711509 0.249834479 Leu343Ala 30C 253 262 2 FFKDGKYDLD 30 1 4.593914676 0.70054452 Leu343Ala 30C 253 262 2 FFKDGKYDLD 60 1 4.685299137 0.698024576 Leu343Ala 30C 253 262 2 FFKDGKYDLD 90 1 5.049055112 0.242634638 Leu343Ala 30C 253 262 2 FFKDGKYDLD 120 1 5.338736217 0.08279817 Leu343Ala 30C 253 262 2 FFKDGKYDLD 180 1 5.322953942 0.0950379 Leu343Ala 30C 253 262 2 FFKDGKYDLD 240 1 5.541869364 0.06695852 Leu343Ala 40C 253 262 2 FFKDGKYDLD 0 1 2.89E-13 0.099717797 Leu343Ala 40C 253 262 2 FFKDGKYDLD 0.166666667 1 2.965285757 0.472309563 Leu343Ala 40C 253 262 2 FFKDGKYDLD 0.333333333 1 3.073470704 0.165236349 Leu343Ala 40C 253 262 2 FFKDGKYDLD 0.5 1 3.101726067 0.601186715 Leu343Ala 40C 253 262 2 FFKDGKYDLD 0.75 1 3.239184588 0.570947384 Leu343Ala 40C 253 262 2 FFKDGKYDLD 1 1 3.198456137 0.635745952 Leu343Ala 40C 253 262 2 FFKDGKYDLD 3 1 3.738362661 0.350992244 Leu343Ala 40C 253 262 2 FFKDGKYDLD 5 1 4.070808639 0.327232769 Leu343Ala 40C 253 262 2 FFKDGKYDLD 10 1 4.432528191 0.393831297 Leu343Ala 40C 253 262 2 FFKDGKYDLD 30 1 5.167931277 0.323632849 Leu343Ala 40C 253 262 2 FFKDGKYDLD 60 1 5.477212949 Leu343Ala 40C 253 262 2 FFKDGKYDLD 90 1 6.190215387 0.081718194 Leu343Ala 40C 253 262 2 FFKDGKYDLD 120 1 6.453168447 0.097197852 Leu343Ala 40C 253 262 2 FFKDGKYDLD 180 1 6.358983905 Leu343Ala 40C 253 262 2 FFKDGKYDLD 240 1 6.898381323 0.174596142 WT 10C 253 262 2 FFKDGKYDLD 0 1 0 0.066598528 WT 10C 253 262 2 FFKDGKYDLD 0.166666667 1 2.050677488 0.222835076 WT 10C 253 262 2 FFKDGKYDLD 0.333333333 1 2.002312453 0.050758878 WT 10C 253 262 2 FFKDGKYDLD 0.5 1 2.303702988 0.047158958 WT 10C 253 262 2 FFKDGKYDLD 0.75 1 2.375741435 0.273593954 WT 10C 253 262 2 FFKDGKYDLD 1 1 2.213336738 0.107997614 WT 10C 253 262 2 FFKDGKYDLD 3 1 2.648622054 0.018719586 WT 10C 253 262 2 FFKDGKYDLD 5 1 2.6257123 0.160556452 WT 10C 253 262 2 FFKDGKYDLD 10 1 2.940085029 0.23939471 WT 10C 253 262 2 FFKDGKYDLD 30 1 3.276858405 0.115917439 WT 10C 253 262 2 FFKDGKYDLD 60 1 3.709852745 0.214195267 WT 10C 253 262 2 FFKDGKYDLD 90 1 3.674979009 0.010079777 WT 10C 253 262 2 FFKDGKYDLD 120 1 3.801237206 0.205195466 WT 10C 253 262 2 FFKDGKYDLD 180 1 4.320015846 0.179996023 WT 10C 253 262 2 FFKDGKYDLD 240 1 4.235249758 0.005399881 WT 20C 253 262 2 FFKDGKYDLD 0 1 -2.89E-13 0.009359793 WT 20C 253 262 2 FFKDGKYDLD 0.166666667 1 2.142825608 0.337672538 WT 20C 253 262 2 FFKDGKYDLD 0.333333333 1 2.446507118 0.12851716 WT 20C 253 262 2 FFKDGKYDLD 0.5 1 2.384650783 0.317512984 WT 20C 253 262 2 FFKDGKYDLD 0.75 1 2.703860015 0.329032729 WT 20C 253 262 2 FFKDGKYDLD 1 1 2.133916259 0.186115887 WT 20C 253 262 2 FFKDGKYDLD 3 1 2.916157064 0.481669356 WT 20C 253 262 2 FFKDGKYDLD 5 1 2.933975761 0.639345872 WT 20C 253 262 2 FFKDGKYDLD 10 1 3.459627327 0.050038894 WT 20C 253 262 2 FFKDGKYDLD 30 1 3.857493379 0.010079777 WT 20C 253 262 2 FFKDGKYDLD 60 1 4.198085047 0.044639014 WT 20C 253 262 2 FFKDGKYDLD 90 1 4.734936937 0.254514376 WT 20C 253 262 2 FFKDGKYDLD 120 1 4.425146159 0.52270845 WT 20C 253 262 2 FFKDGKYDLD 180 1 4.735191489 0.354232172 WT 20C 253 262 2 FFKDGKYDLD 240 1 5.110656893 0.420830701 WT 25C 253 262 2 FFKDGKYDLD 0 1 0 0.03347926 WT 25C 253 262 2 FFKDGKYDLD 0.166666667 1 2.94924893 0.110517558 WT 25C 253 262 2 FFKDGKYDLD 0.333333333 1 2.593638646 0.100797773 WT 25C 253 262 2 FFKDGKYDLD 0.5 1 3.208129144 0.116637423 WT 25C 253 262 2 FFKDGKYDLD 0.75 1 2.877210483 0.669585204 WT 25C 253 262 2 FFKDGKYDLD 1 1 3.153909394 0.427310558 WT 25C 253 262 2 FFKDGKYDLD 3 1 3.356533436 0.533148219 WT 25C 253 262 2 FFKDGKYDLD 5 1 3.212711095 0.074878345 WT 25C 253 262 2 FFKDGKYDLD 10 1 3.969751171 0.05975868 WT 25C 253 262 2 FFKDGKYDLD 30 1 4.60053305 0.106557645 WT 25C 253 262 2 FFKDGKYDLD 60 1 4.879013831 WT 25C 253 262 2 FFKDGKYDLD 90 1 5.083674295 0.198715609 WT 25C 253 262 2 FFKDGKYDLD 120 1 5.429102467 0.02987934 WT 25C 253 262 2 FFKDGKYDLD 180 1 5.347645565 0.255954344 WT 25C 253 262 2 FFKDGKYDLD 240 1 5.845550874 0.06155864 WT 30C 253 262 2 FFKDGKYDLD 0 1 0 0.019799562 WT 30C 253 262 2 FFKDGKYDLD 0.166666667 1 2.684768554 0.132477073 WT 30C 253 262 2 FFKDGKYDLD 0.333333333 1 2.637167177 0.01403969 WT 30C 253 262 2 FFKDGKYDLD 0.5 1 2.730078955 0.083518154 WT 30C 253 262 2 FFKDGKYDLD 0.75 1 2.736697328 0.123117279 WT 30C 253 262 2 FFKDGKYDLD 1 1 2.993795673 0.033839252 WT 30C 253 262 2 FFKDGKYDLD 3 1 3.141181753 0.198355617 WT 30C 253 262 2 FFKDGKYDLD 5 1 3.515628946 0.228234957 WT 30C 253 262 2 FFKDGKYDLD 10 1 4.030080188 0.127077192 WT 30C 253 262 2 FFKDGKYDLD 30 1 4.591114595 0.149396699 WT 30C 253 262 2 FFKDGKYDLD 60 1 5.063564622 0.009719785 WT 30C 253 262 2 FFKDGKYDLD 90 1 5.189313714 0.268194074 WT 30C 253 262 2 FFKDGKYDLD 120 1 5.553324241 0.024119467 WT 30C 253 262 2 FFKDGKYDLD 180 1 5.952208504 0.202315529 WT 30C 253 262 2 FFKDGKYDLD 240 1 6.240107739 0.236514774 WT 40C 253 262 2 FFKDGKYDLD 0 1 2.89E-13 0.014759674 WT 40C 253 262 2 FFKDGKYDLD 0.166666667 1 2.706405543 0.236514774 WT 40C 253 262 2 FFKDGKYDLD 0.333333333 1 2.986159088 0.486709245 WT 40C 253 262 2 FFKDGKYDLD 0.5 1 2.917684381 0.396351242 WT 40C 253 262 2 FFKDGKYDLD 0.75 1 3.084416475 0.122397295 WT 40C 253 262 2 FFKDGKYDLD 1 1 3.371552052 WT 40C 253 262 2 FFKDGKYDLD 3 1 4.194266755 0.488149213 WT 40C 253 262 2 FFKDGKYDLD 5 1 4.500748346 0.117357407 WT 40C 253 262 2 FFKDGKYDLD 10 1 4.922542362 0.234354821 WT 40C 253 262 2 FFKDGKYDLD 30 1 6.017883131 0.101517757 WT 40C 253 262 2 FFKDGKYDLD 60 1 6.758631826 0.108717598 WT 40C 253 262 2 FFKDGKYDLD 90 1 7.32297542 0.030599324 WT 40C 253 262 2 FFKDGKYDLD 180 1 7.861863732 0.398871186 WT 40C 253 262 2 FFKDGKYDLD 240 1 7.470106948 0.019799562 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 2.57E-13 0.140739985 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 3.798202496 0.608700436 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 4.115529841 0.314106058 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 4.463617356 0.261968291 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 4.603621367 0.334577329 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 4.777778213 0.967267535 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 5.360864382 0.507303674 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 5.406552282 0.41902132 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 6.053872924 0.326900602 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 6.594211504 0.192877753 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 7.341728877 0.194477071 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 7.840224578 0.445250135 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 8.174515252 0.623094298 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 8.430774611 0.1775243 Leu343Ala 10C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 8.848524865 0.463162497 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.025269225 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 4.927734043 0.420940501 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 4.854000106 0.085083718 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.581387662 0.080605628 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 5.302962885 0.22550384 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 5.520319676 0.323062239 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 6.104762912 0.492589948 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 6.384318577 0.008956181 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 6.828531624 0.241816884 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 7.996287206 0.417422002 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 9.137127639 0.092440581 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 9.221944285 0.193197616 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 9.532260121 0.520737945 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 9.856598975 0.424778865 Leu343Ala 20C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.10019238 0.262608018 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 -2.57E-13 0.030706906 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 5.032454329 0.328819784 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 4.906020982 0.102676217 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.439574229 0.337136237 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 5.338698965 0.230941521 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 5.507427546 0.397270595 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 6.319179393 0.245335383 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 6.729013426 0.440452181 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 6.884623699 0.218466841 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 8.359980984 0.040942541 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 9.261299209 0.010555499 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 9.387054023 0.041902132 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 9.855468086 0.119628987 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 9.966747526 0.324981421 Leu343Ala 25C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 9.874693193 0.637488161 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.172726346 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 5.073844852 0.455805634 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 5.371268557 0.394071959 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.868407192 0.355688326 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 5.918166291 0.30898824 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 6.218530306 0.51050231 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 6.759095063 0.650602568 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 7.335169723 0.924085949 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 7.989049519 1.079859523 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 9.880799991 0.449728226 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 10.12077455 0.30802865 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 10.17505721 0.668834794 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 10.59054568 0.946156537 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 10.18908023 0.656040249 Leu343Ala 30C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.06897986 0.819490551 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.003198636 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 6.007732669 0.109393352 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 6.134166016 0.338735555 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 6.28366949 0.36400478 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 7.06828001 0.217187386 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 7.139299815 0.16600921 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 8.276521405 0.224864113 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 8.929722668 0.11099267 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 9.578626554 0.548246215 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 10.51228819 0.696662927 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 10.60683048 0.354408872 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 10.54666721 0.355048599 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 10.68440944 0.678430702 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 10.64844718 0.257810064 Leu343Ala 40C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.78505853 0.417741865 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 -2.57E-13 0.087962491 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 3.897494517 0.261328564 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 4.285389311 0.3480116 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 4.31230446 0.301631377 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 4.901949783 0.626292934 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 4.580098883 0.081565219 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 5.573019086 0.254291564 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 5.489333328 0.288197106 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 6.194781645 0.633969661 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 6.178496849 0.41198432 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 7.26595934 0.283399152 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 7.582381974 0.699541699 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 8.153480723 0.525216036 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 8.668487398 0.075807674 WT 10C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 8.511293881 0.220705886 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 2.57E-13 0.050858313 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 4.653380466 0.041902132 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 4.844726819 0.178803754 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.173136872 0.080285764 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 5.408587881 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 5.01051509 0.047339813 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 5.588399171 0.355368463 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 5.802815652 0.94199831 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 6.511430457 0.088922082 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 7.4744952 0.674912202 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 8.659440289 0.041262405 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 9.552616116 0.180403072 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 9.322819549 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 9.735820071 0.046060359 WT 20C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.08730025 0.211749705 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.037743905 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 5.966794501 0.638767615 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 5.497249549 0.402068549 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.790375877 0.272843653 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 6.021981865 0.647723796 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 6.195460179 0.037743905 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 6.567296355 0.273803244 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 6.809306518 0.481714586 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 7.72871896 0.429576819 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 8.975410567 0.234460021 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 9.729713273 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 10.30420469 0.168887982 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 10.28136074 0.418061729 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 10.03233907 0.87450709 WT 25C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.37047476 0.428297364 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.112911852 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 5.210230019 0.025269225 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 5.230586014 0.070050129 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 5.711892208 0.072609038 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 5.718903717 0.138181076 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 6.074907452 0.300671787 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 6.55779689 0.077726855 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 7.399178018 0.411664457 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 8.320173705 0.788463781 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 9.731296517 0.273163517 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 10.19993676 0.280840243 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 10.26190945 0.090841263 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 120 1 10.34310726 0.151295484 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 10.01808987 0.47851595 WT 30C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.24132728 0.156413302 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 0 1 0 0.055656267 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.166666667 1 5.995971427 0.367843143 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.333333333 1 6.522965521 0.399829504 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.5 1 6.663874242 0.084763855 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 0.75 1 6.924883334 0.445569999 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 1 1 7.535337007 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 3 1 8.558112669 0.065252175 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 5 1 9.05954868 0.215268205 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 10 1 9.874693193 0.332338283 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 30 1 10.73032352 0.150975621 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 60 1 10.54033423 0.93400172 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 90 1 10.8219255 0.509542719 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 180 1 10.95243004 WT 40C 263 279 2 FKNPNSDKSKWLTGPQL 240 1 10.50708611 0.195436661 Leu343Ala 10C 280 286 2 ADLYHSL 0 1 -1.15E-13 0.00228801 Leu343Ala 10C 280 286 2 ADLYHSL 0.166666667 1 0.714288603 0.089232409 Leu343Ala 10C 280 286 2 ADLYHSL 0.333333333 1 0.783452449 0.06978432 Leu343Ala 10C 280 286 2 ADLYHSL 0.5 1 0.844931423 Leu343Ala 10C 280 286 2 ADLYHSL 0.75 1 0.684560284 0.027170125 Leu343Ala 10C 280 286 2 ADLYHSL 1 1 0.828954979 0.345203582 Leu343Ala 10C 280 286 2 ADLYHSL 3 1 0.95797993 0.324039485 Leu343Ala 10C 280 286 2 ADLYHSL 5 1 0.871221773 Leu343Ala 10C 280 286 2 ADLYHSL 10 1 1.160820097 0.110396506 Leu343Ala 10C 280 286 2 ADLYHSL 30 1 1.017841036 0.234807076 Leu343Ala 10C 280 286 2 ADLYHSL 60 1 0.998831091 0.102102468 Leu343Ala 10C 280 286 2 ADLYHSL 90 1 1.066579302 0.324897489 Leu343Ala 10C 280 286 2 ADLYHSL 120 1 1.185290347 0.288575323 Leu343Ala 10C 280 286 2 ADLYHSL 180 1 1.237264347 0.167024766 Leu343Ala 10C 280 286 2 ADLYHSL 240 1 1.310068395 0.004576021 Leu343Ala 20C 280 286 2 ADLYHSL 0 1 0 0.005148024 Leu343Ala 20C 280 286 2 ADLYHSL 0.166666667 1 0.694469724 Leu343Ala 20C 280 286 2 ADLYHSL 0.333333333 1 1.090645084 0.059774274 Leu343Ala 20C 280 286 2 ADLYHSL 0.5 1 1.120777871 0.273417253 Leu343Ala 20C 280 286 2 ADLYHSL 0.75 1 0.976989876 0.227943045 Leu343Ala 20C 280 286 2 ADLYHSL 1 1 1.019661138 0.086944399 Leu343Ala 20C 280 286 2 ADLYHSL 3 1 1.278519975 Leu343Ala 20C 280 286 2 ADLYHSL 5 1 1.270228402 0.217646998 Leu343Ala 20C 280 286 2 ADLYHSL 10 1 1.189941716 0.23566508 Leu343Ala 20C 280 286 2 ADLYHSL 30 1 1.27649764 0.080080367 Leu343Ala 20C 280 286 2 ADLYHSL 60 1 1.243331351 0.196196899 Leu343Ala 20C 280 286 2 ADLYHSL 90 1 1.344043618 0.109252501 Leu343Ala 20C 280 286 2 ADLYHSL 120 1 1.489853947 0.040326185 Leu343Ala 20C 280 286 2 ADLYHSL 180 1 1.550523987 0.109538502 Leu343Ala 20C 280 286 2 ADLYHSL 240 1 1.482775776 0.049192225 Leu343Ala 25C 280 286 2 ADLYHSL 0 1 1.15E-13 0.016302075 Leu343Ala 25C 280 286 2 ADLYHSL 0.166666667 1 1.006515962 Leu343Ala 25C 280 286 2 ADLYHSL 0.333333333 1 0.964249168 0.107822494 Leu343Ala 25C 280 286 2 ADLYHSL 0.5 1 1.194188619 0.158444726 Leu343Ala 25C 280 286 2 ADLYHSL 0.75 1 0.998022157 0.187044857 Leu343Ala 25C 280 286 2 ADLYHSL 1 1 0.939981152 0.188474864 Leu343Ala 25C 280 286 2 ADLYHSL 3 1 1.313101897 0.376949728 Leu343Ala 25C 280 286 2 ADLYHSL 5 1 1.037053216 0.100958463 Leu343Ala 25C 280 286 2 ADLYHSL 10 1 0.990135052 0.035750164 Leu343Ala 25C 280 286 2 ADLYHSL 30 1 1.260925663 0.090948417 Leu343Ala 25C 280 286 2 ADLYHSL 60 1 1.318359967 0.078936362 Leu343Ala 25C 280 286 2 ADLYHSL 90 1 1.300361189 0.159874733 Leu343Ala 25C 280 286 2 ADLYHSL 120 1 1.579038906 0.177606814 Leu343Ala 25C 280 286 2 ADLYHSL 180 1 1.541221247 0.111540511 Leu343Ala 25C 280 286 2 ADLYHSL 240 1 1.599464486 0.126984582 Leu343Ala 30C 280 286 2 ADLYHSL 0 1 0 0.007722035 Leu343Ala 30C 280 286 2 ADLYHSL 0.166666667 1 0.791339554 0.006292029 Leu343Ala 30C 280 286 2 ADLYHSL 0.333333333 1 0.855649796 0.018876087 Leu343Ala 30C 280 286 2 ADLYHSL 0.5 1 Leu343Ala 30C 280 286 2 ADLYHSL 0.75 1 0.818641072 0.037466172 Leu343Ala 30C 280 286 2 ADLYHSL 1 1 1.049591691 0.27484726 Leu343Ala 30C 280 286 2 ADLYHSL 3 1 0.915713136 0.19419489 Leu343Ala 30C 280 286 2 ADLYHSL 5 1 1.080128944 0.325469492 Leu343Ala 30C 280 286 2 ADLYHSL 10 1 1.12421584 0.104104477 Leu343Ala 30C 280 286 2 ADLYHSL 30 1 1.190548417 Leu343Ala 30C 280 286 2 ADLYHSL 60 1 1.085387014 0.031460144 Leu343Ala 30C 280 286 2 ADLYHSL 90 1 1.325640372 0.038324176 Leu343Ala 30C 280 286 2 ADLYHSL 120 1 1.326449306 0.286001311 Leu343Ala 30C 280 286 2 ADLYHSL 180 1 1.431206242 0.060632278 Leu343Ala 30C 280 286 2 ADLYHSL 240 1 1.377007673 0.145860669 Leu343Ala 40C 280 286 2 ADLYHSL 0 1 0 0.005434025 Leu343Ala 40C 280 286 2 ADLYHSL 0.166666667 1 1.085387014 0.074932343 Leu343Ala 40C 280 286 2 ADLYHSL 0.333333333 1 1.098734423 0.146432671 Leu343Ala 40C 280 286 2 ADLYHSL 0.5 1 1.102576859 0.154726709 Leu343Ala 40C 280 286 2 ADLYHSL 0.75 1 1.114104166 0.008008037 Leu343Ala 40C 280 286 2 ADLYHSL 1 1 1.091251785 0.251395152 Leu343Ala 40C 280 286 2 ADLYHSL 3 1 1.021481239 0.088660406 Leu343Ala 40C 280 286 2 ADLYHSL 5 1 1.187717148 0.04804822 Leu343Ala 40C 280 286 2 ADLYHSL 10 1 1.367098233 0.001430007 Leu343Ala 40C 280 286 2 ADLYHSL 30 1 1.453654157 0.191334877 Leu343Ala 40C 280 286 2 ADLYHSL 60 1 1.545670384 0.137280629 Leu343Ala 40C 280 286 2 ADLYHSL 90 1 1.538187745 0.163306749 Leu343Ala 40C 280 286 2 ADLYHSL 120 1 1.622114634 0.129272593 Leu343Ala 40C 280 286 2 ADLYHSL 180 1 1.910701791 0.199342914 Leu343Ala 40C 280 286 2 ADLYHSL 240 1 2.015458726 0.153582704 WT 10C 280 286 2 ADLYHSL 0 1 1.15E-13 0.002002009 WT 10C 280 286 2 ADLYHSL 0.166666667 1 0.633395217 0.132418607 WT 10C 280 286 2 ADLYHSL 0.333333333 1 0.57697208 0.085800393 WT 10C 280 286 2 ADLYHSL 0.5 1 0.825112543 0.257687181 WT 10C 280 286 2 ADLYHSL 0.75 1 0.762217935 0.140712645 WT 10C 280 286 2 ADLYHSL 1 1 0.544816958 0.038610177 WT 10C 280 286 2 ADLYHSL 3 1 0.798619959 0.392393799 WT 10C 280 286 2 ADLYHSL 5 1 0.712872969 0.151008692 WT 10C 280 286 2 ADLYHSL 10 1 0.73390525 0.401545841 WT 10C 280 286 2 ADLYHSL 30 1 0.917533237 0.055484254 WT 10C 280 286 2 ADLYHSL 60 1 1.087611582 0.047190216 WT 10C 280 286 2 ADLYHSL 90 1 1.042109052 0.008008037 WT 10C 280 286 2 ADLYHSL 120 1 1.040491185 0.121264556 WT 10C 280 286 2 ADLYHSL 180 1 1.325842606 0.246819131 WT 10C 280 286 2 ADLYHSL 240 1 1.105205894 0.03489216 WT 20C 280 286 2 ADLYHSL 0 1 0 0.015444071 WT 20C 280 286 2 ADLYHSL 0.166666667 1 0.664134704 0.345489584 WT 20C 280 286 2 ADLYHSL 0.333333333 1 0.75554423 0.155584713 WT 20C 280 286 2 ADLYHSL 0.5 1 0.740174487 0.439870016 WT 20C 280 286 2 ADLYHSL 0.75 1 0.85059396 0.025168115 WT 20C 280 286 2 ADLYHSL 1 1 0.590723955 0.136994628 WT 20C 280 286 2 ADLYHSL 3 1 0.932700747 0.465038132 WT 20C 280 286 2 ADLYHSL 5 1 0.828348278 0.537110462 WT 20C 280 286 2 ADLYHSL 10 1 0.902163494 0.265123215 WT 20C 280 286 2 ADLYHSL 30 1 1.097925489 0.034034156 WT 20C 280 286 2 ADLYHSL 60 1 1.215625367 0.133562612 WT 20C 280 286 2 ADLYHSL 90 1 1.417656599 0.183040839 WT 20C 280 286 2 ADLYHSL 120 1 1.250409523 0.20735095 WT 20C 280 286 2 ADLYHSL 180 1 1.243938052 0.169598777 WT 20C 280 286 2 ADLYHSL 240 1 1.383479144 0.25311116 WT 25C 280 286 2 ADLYHSL 0 1 1.15E-13 0.000286001 WT 25C 280 286 2 ADLYHSL 0.166666667 1 0.892658521 0.500216293 WT 25C 280 286 2 ADLYHSL 0.333333333 1 0.66797714 0.176176808 WT 25C 280 286 2 ADLYHSL 0.5 1 1.105205894 0.319749466 WT 25C 280 286 2 ADLYHSL 0.75 1 0.739567787 0.043472199 WT 25C 280 286 2 ADLYHSL 1 1 1.047771589 0.537110462 WT 25C 280 286 2 ADLYHSL 3 1 1.185290347 0.465038132 WT 25C 280 286 2 ADLYHSL 5 1 1.094689754 WT 25C 280 286 2 ADLYHSL 10 1 1.18488588 0.313457437 WT 25C 280 286 2 ADLYHSL 30 1 1.5203912 0.27484726 WT 25C 280 286 2 ADLYHSL 60 1 1.295709819 WT 25C 280 286 2 ADLYHSL 90 1 1.59501535 0.240241101 WT 25C 280 286 2 ADLYHSL 120 1 1.55477089 0.13299061 WT 25C 280 286 2 ADLYHSL 180 1 1.447384919 0.197340905 WT 25C 280 286 2 ADLYHSL 240 1 1.693300814 0.227085041 WT 30C 280 286 2 ADLYHSL 0 1 0 0.006864031 WT 30C 280 286 2 ADLYHSL 0.166666667 1 0.599824461 WT 30C 280 286 2 ADLYHSL 0.333333333 1 0.680515614 0.168454772 WT 30C 280 286 2 ADLYHSL 0.5 1 0.749881693 0.307737411 WT 30C 280 286 2 ADLYHSL 0.75 1 0.83138178 0.373803713 WT 30C 280 286 2 ADLYHSL 1 1 0.898927758 0.254255165 WT 30C 280 286 2 ADLYHSL 3 1 0.903579128 0.083512383 WT 30C 280 286 2 ADLYHSL 5 1 1.048176056 0.037466172 WT 30C 280 286 2 ADLYHSL 10 1 1.032604079 0.097812448 WT 30C 280 286 2 ADLYHSL 30 1 1.289036115 0.279137279 WT 30C 280 286 2 ADLYHSL 60 1 1.293889718 0.013728063 WT 30C 280 286 2 ADLYHSL 90 1 1.386917113 0.136136624 WT 30C 280 286 2 ADLYHSL 120 1 1.513919729 0.089804412 WT 30C 280 286 2 ADLYHSL 180 1 1.747701617 0.095524438 WT 30C 280 286 2 ADLYHSL 240 1 1.895938748 0.170742783 WT 40C 280 286 2 ADLYHSL 0 1 0 0.001430007 WT 40C 280 286 2 ADLYHSL 0.166666667 1 0.850189493 0.252539158 WT 40C 280 286 2 ADLYHSL 0.333333333 1 0.462103471 WT 40C 280 286 2 ADLYHSL 0.5 1 0.922993541 0.206778948 WT 40C 280 286 2 ADLYHSL 0.75 1 0.802057928 0.32289548 WT 40C 280 286 2 ADLYHSL 1 1 0.765049203 WT 40C 280 286 2 ADLYHSL 3 1 1.089027217 WT 40C 280 286 2 ADLYHSL 5 1 1.399455588 0.002574012 WT 40C 280 286 2 ADLYHSL 10 1 1.239286682 0.141570649 WT 40C 280 286 2 ADLYHSL 30 1 1.592184081 0.276277266 WT 40C 280 286 2 ADLYHSL 60 1 1.546883785 WT 40C 280 286 2 ADLYHSL 90 1 2.07167963 0.20735095 WT 40C 280 286 2 ADLYHSL 180 1 2.46259692 0.163020747 WT 40C 280 286 2 ADLYHSL 240 1 2.653303079 0.067782311 Leu343Ala 10C 287 294 2 MKRYPIVS 0 1 8.08E-14 0.005025902 Leu343Ala 10C 287 294 2 MKRYPIVS 0.166666667 1 0.400021323 0.019098429 Leu343Ala 10C 287 294 2 MKRYPIVS 0.333333333 1 0.46797093 0.047243483 Leu343Ala 10C 287 294 2 MKRYPIVS 0.5 1 0.544307621 0.045032086 Leu343Ala 10C 287 294 2 MKRYPIVS 0.75 1 0.527391297 0.035181317 Leu343Ala 10C 287 294 2 MKRYPIVS 1 1 0.678358832 0.129266211 Leu343Ala 10C 287 294 2 MKRYPIVS 3 1 0.828046982 0.04945488 Leu343Ala 10C 287 294 2 MKRYPIVS 5 1 0.830890062 0.082826872 Leu343Ala 10C 287 294 2 MKRYPIVS 10 1 0.969916662 0.095291111 Leu343Ala 10C 287 294 2 MKRYPIVS 30 1 0.991808376 0.127858958 Leu343Ala 10C 287 294 2 MKRYPIVS 60 1 0.983279137 0.030557487 Leu343Ala 10C 287 294 2 MKRYPIVS 90 1 0.939922171 0.010252841 Leu343Ala 10C 287 294 2 MKRYPIVS 120 1 1.032180109 0.028547126 Leu343Ala 10C 287 294 2 MKRYPIVS 180 1 0.982426213 0.110971926 Leu343Ala 10C 287 294 2 MKRYPIVS 240 1 1.117898964 0.058099432 Leu343Ala 20C 287 294 2 MKRYPIVS 0 1 0 0.007438336 Leu343Ala 20C 287 294 2 MKRYPIVS 0.166666667 1 0.634859712 0.146555316 Leu343Ala 20C 287 294 2 MKRYPIVS 0.333333333 1 0.725269648 0.07056367 Leu343Ala 20C 287 294 2 MKRYPIVS 0.5 1 0.80302788 0.083228945 Leu343Ala 20C 287 294 2 MKRYPIVS 0.75 1 0.810562041 0.033170956 Leu343Ala 20C 287 294 2 MKRYPIVS 1 1 0.908932601 0.011459058 Leu343Ala 20C 287 294 2 MKRYPIVS 3 1 1.058052135 0.004824866 Leu343Ala 20C 287 294 2 MKRYPIVS 5 1 0.956980649 0.111977107 Leu343Ala 20C 287 294 2 MKRYPIVS 10 1 1.064591218 0.101322193 Leu343Ala 20C 287 294 2 MKRYPIVS 30 1 1.065301988 0.050057988 Leu343Ala 20C 287 294 2 MKRYPIVS 60 1 1.065159834 0.033371992 Leu343Ala 20C 287 294 2 MKRYPIVS 90 1 1.166373474 0.065135696 Leu343Ala 20C 287 294 2 MKRYPIVS 120 1 1.247827709 0.095894219 Leu343Ala 20C 287 294 2 MKRYPIVS 180 1 1.371501679 0.064532588 Leu343Ala 20C 287 294 2 MKRYPIVS 240 1 1.427226043 0.057295288 Leu343Ala 25C 287 294 2 MKRYPIVS 0 1 0 0.011660094 Leu343Ala 25C 287 294 2 MKRYPIVS 0.166666667 1 0.737352737 0.011056985 Leu343Ala 25C 287 294 2 MKRYPIVS 0.333333333 1 0.735789043 0.082022728 Leu343Ala 25C 287 294 2 MKRYPIVS 0.5 1 0.90566306 0.004221758 Leu343Ala 25C 287 294 2 MKRYPIVS 0.75 1 0.860315937 0.058300468 Leu343Ala 25C 287 294 2 MKRYPIVS 1 1 0.911491373 0.006835227 Leu343Ala 25C 287 294 2 MKRYPIVS 3 1 1.062174601 0.02010361 Leu343Ala 25C 287 294 2 MKRYPIVS 5 1 0.948593564 0.093883858 Leu343Ala 25C 287 294 2 MKRYPIVS 10 1 1.00119054 0.009046624 Leu343Ala 25C 287 294 2 MKRYPIVS 30 1 1.075252768 0.046640375 Leu343Ala 25C 287 294 2 MKRYPIVS 60 1 1.16125593 0.110368818 Leu343Ala 25C 287 294 2 MKRYPIVS 90 1 1.172059634 0.078605114 Leu343Ala 25C 287 294 2 MKRYPIVS 120 1 1.335252412 0.194803979 Leu343Ala 25C 287 294 2 MKRYPIVS 180 1 1.402206941 0.07880615 Leu343Ala 25C 287 294 2 MKRYPIVS 240 1 1.510243972 0.164849601 Leu343Ala 30C 287 294 2 MKRYPIVS 0 1 0 0.012665274 Leu343Ala 30C 287 294 2 MKRYPIVS 0.166666667 1 0.698829007 0.060109793 Leu343Ala 30C 287 294 2 MKRYPIVS 0.333333333 1 0.839845763 0.003819686 Leu343Ala 30C 287 294 2 MKRYPIVS 0.5 1 0.989960375 0.014675635 Leu343Ala 30C 287 294 2 MKRYPIVS 0.75 1 0.923148 0.05769736 Leu343Ala 30C 287 294 2 MKRYPIVS 1 1 1.011283473 0.032768884 Leu343Ala 30C 287 294 2 MKRYPIVS 3 1 0.932956625 0.036186498 Leu343Ala 30C 287 294 2 MKRYPIVS 5 1 0.974749898 0.055888035 Leu343Ala 30C 287 294 2 MKRYPIVS 10 1 0.97162251 0.043423797 Leu343Ala 30C 287 294 2 MKRYPIVS 30 1 1.062032447 Leu343Ala 30C 287 294 2 MKRYPIVS 60 1 1.200206123 0.081218584 Leu343Ala 30C 287 294 2 MKRYPIVS 90 1 1.432059278 0.013469419 Leu343Ala 30C 287 294 2 MKRYPIVS 120 1 1.513655668 0.0484497 Leu343Ala 30C 287 294 2 MKRYPIVS 180 1 1.730298346 0.050862133 Leu343Ala 30C 287 294 2 MKRYPIVS 240 1 1.832222755 0.04945488 Leu343Ala 40C 287 294 2 MKRYPIVS 0 1 0 0.002412433 Leu343Ala 40C 287 294 2 MKRYPIVS 0.166666667 1 0.924711694 0.099512869 Leu343Ala 40C 287 294 2 MKRYPIVS 0.333333333 1 0.963519733 0.083228945 Leu343Ala 40C 287 294 2 MKRYPIVS 0.5 1 0.974749898 0.155400904 Leu343Ala 40C 287 294 2 MKRYPIVS 0.75 1 0.984558523 0.143539774 Leu343Ala 40C 287 294 2 MKRYPIVS 1 1 1.011567781 0.151179146 Leu343Ala 40C 287 294 2 MKRYPIVS 3 1 0.997920998 0.085239306 Leu343Ala 40C 287 294 2 MKRYPIVS 5 1 1.010004087 0.081017548 Leu343Ala 40C 287 294 2 MKRYPIVS 10 1 1.132256517 0.163442348 Leu343Ala 40C 287 294 2 MKRYPIVS 30 1 1.420402651 0.209881687 Leu343Ala 40C 287 294 2 MKRYPIVS 60 1 1.798816568 0.265769722 Leu343Ala 40C 287 294 2 MKRYPIVS 90 1 1.911971143 0.060310829 Leu343Ala 40C 287 294 2 MKRYPIVS 120 1 2.008209393 0.017088068 Leu343Ala 40C 287 294 2 MKRYPIVS 180 1 2.198695737 0.136503511 Leu343Ala 40C 287 294 2 MKRYPIVS 240 1 2.197132043 WT 10C 287 294 2 MKRYPIVS 0 1 8.08E-14 0.055083891 WT 10C 287 294 2 MKRYPIVS 0.166666667 1 0.610693534 0.043021725 WT 10C 287 294 2 MKRYPIVS 0.333333333 1 0.643388951 0.031361631 WT 10C 287 294 2 MKRYPIVS 0.5 1 0.759813068 0.033975101 WT 10C 287 294 2 MKRYPIVS 0.75 1 0.837002683 0.099311833 WT 10C 287 294 2 MKRYPIVS 1 1 0.807718962 0.02010361 WT 10C 287 294 2 MKRYPIVS 3 1 1.090747552 0.000603108 WT 10C 287 294 2 MKRYPIVS 5 1 1.095012172 0.018696357 WT 10C 287 294 2 MKRYPIVS 10 1 1.164951934 0.073780248 WT 10C 287 294 2 MKRYPIVS 30 1 1.170211632 0.031763704 WT 10C 287 294 2 MKRYPIVS 60 1 1.31663024 0.060310829 WT 10C 287 294 2 MKRYPIVS 90 1 1.306395153 0.018495321 WT 10C 287 294 2 MKRYPIVS 120 1 1.315066546 0.088254847 WT 10C 287 294 2 MKRYPIVS 180 1 1.488494412 0.020304646 WT 10C 287 294 2 MKRYPIVS 240 1 1.485082716 0.008644552 WT 20C 287 294 2 MKRYPIVS 0 1 0 0.011660094 WT 20C 287 294 2 MKRYPIVS 0.166666667 1 0.599463369 0.056893216 WT 20C 287 294 2 MKRYPIVS 0.333333333 1 0.752989676 0.027944018 WT 20C 287 294 2 MKRYPIVS 0.5 1 0.820939282 0.022315007 WT 20C 287 294 2 MKRYPIVS 0.75 1 0.941059403 0.107353276 WT 20C 287 294 2 MKRYPIVS 1 1 0.816674663 0.005025902 WT 20C 287 294 2 MKRYPIVS 3 1 0.91888338 0.14836464 WT 20C 287 294 2 MKRYPIVS 5 1 0.954279724 0.192793618 WT 20C 287 294 2 MKRYPIVS 10 1 1.039998578 0.033371992 WT 20C 287 294 2 MKRYPIVS 30 1 1.043836736 0.026737801 WT 20C 287 294 2 MKRYPIVS 60 1 1.202480587 0.123235128 WT 20C 287 294 2 MKRYPIVS 90 1 1.356291202 0.009448697 WT 20C 287 294 2 MKRYPIVS 120 1 1.265454804 0.102930482 WT 20C 287 294 2 MKRYPIVS 180 1 1.409598948 0.01286631 WT 20C 287 294 2 MKRYPIVS 240 1 1.609893918 0.063326371 WT 25C 287 294 2 MKRYPIVS 0 1 0 0.019299465 WT 25C 287 294 2 MKRYPIVS 0.166666667 1 0.97801944 0.018495321 WT 25C 287 294 2 MKRYPIVS 0.333333333 1 0.913055067 0.014273563 WT 25C 287 294 2 MKRYPIVS 0.5 1 1.09600725 0.033774064 WT 25C 287 294 2 MKRYPIVS 0.75 1 1.047817048 0.194401907 WT 25C 287 294 2 MKRYPIVS 1 1 1.174191943 0.047444519 WT 25C 287 294 2 MKRYPIVS 3 1 1.156138387 0.159421626 WT 25C 287 294 2 MKRYPIVS 5 1 1.139079908 0.017490141 WT 25C 287 294 2 MKRYPIVS 10 1 1.251239405 0.019299465 WT 25C 287 294 2 MKRYPIVS 30 1 1.418412495 0.04945488 WT 25C 287 294 2 MKRYPIVS 60 1 1.504557812 WT 25C 287 294 2 MKRYPIVS 90 1 1.680686604 0.089863136 WT 25C 287 294 2 MKRYPIVS 120 1 1.810899657 0.006232119 WT 25C 287 294 2 MKRYPIVS 180 1 1.831654139 0.1308745 WT 25C 287 294 2 MKRYPIVS 240 1 2.16457878 0.062522227 WT 30C 287 294 2 MKRYPIVS 0 1 8.08E-14 0.008644552 WT 30C 287 294 2 MKRYPIVS 0.166666667 1 0.94276525 0.000201036 WT 30C 287 294 2 MKRYPIVS 0.333333333 1 1.015548092 0.066140876 WT 30C 287 294 2 MKRYPIVS 0.5 1 1.078380155 0.017088068 WT 30C 287 294 2 MKRYPIVS 0.75 1 1.115198038 0.022516043 WT 30C 287 294 2 MKRYPIVS 1 1 1.125006663 0.003819686 WT 30C 287 294 2 MKRYPIVS 3 1 1.132825133 0.053877674 WT 30C 287 294 2 MKRYPIVS 5 1 1.178740871 0.051666277 WT 30C 287 294 2 MKRYPIVS 10 1 1.180446719 0.020706718 WT 30C 287 294 2 MKRYPIVS 30 1 1.385148462 0.070965743 WT 30C 287 294 2 MKRYPIVS 60 1 1.585301278 0.045233122 WT 30C 287 294 2 MKRYPIVS 90 1 1.771523002 0.023521223 WT 30C 287 294 2 MKRYPIVS 120 1 1.912397605 0.076393717 WT 30C 287 294 2 MKRYPIVS 180 1 2.136147982 0.017289104 WT 30C 287 294 2 MKRYPIVS 240 1 2.28185582 0.012263202 WT 40C 287 294 2 MKRYPIVS 0 1 0 0.011056985 WT 40C 287 294 2 MKRYPIVS 0.166666667 1 0.934093857 0.010453877 WT 40C 287 294 2 MKRYPIVS 0.333333333 1 1.029479183 0.089863136 WT 40C 287 294 2 MKRYPIVS 0.5 1 1.021234252 0.038397895 WT 40C 287 294 2 MKRYPIVS 0.75 1 0.992234838 0.018294285 WT 40C 287 294 2 MKRYPIVS 1 1 1.133109441 WT 40C 287 294 2 MKRYPIVS 3 1 1.162535316 0.037191678 WT 40C 287 294 2 MKRYPIVS 5 1 1.21399506 0.044428978 WT 40C 287 294 2 MKRYPIVS 10 1 1.287630826 0.00100518 WT 40C 287 294 2 MKRYPIVS 30 1 1.691916769 0.069759526 WT 40C 287 294 2 MKRYPIVS 60 1 2.041899888 0.161431987 WT 40C 287 294 2 MKRYPIVS 90 1 2.299056453 0.047042447 WT 40C 287 294 2 MKRYPIVS 180 1 2.432112586 0.057094252 WT 40C 287 294 2 MKRYPIVS 240 1 2.62473124 0.087450703 Leu343Ala 10C 295 303 1 IEDPFAEDD 0 1 2.42E-13 0.017774071 Leu343Ala 10C 295 303 1 IEDPFAEDD 0.166666667 1 1.485173824 0.019581604 Leu343Ala 10C 295 303 1 IEDPFAEDD 0.333333333 1 1.327858725 0.065824315 Leu343Ala 10C 295 303 1 IEDPFAEDD 0.5 1 1.36055726 Leu343Ala 10C 295 303 1 IEDPFAEDD 0.75 1 1.250745569 0.00135565 Leu343Ala 10C 295 303 1 IEDPFAEDD 1 1 1.524795501 0.458510788 Leu343Ala 10C 295 303 1 IEDPFAEDD 3 1 1.609151329 0.195213529 Leu343Ala 10C 295 303 1 IEDPFAEDD 5 1 1.294734151 0.120200923 Leu343Ala 10C 295 303 1 IEDPFAEDD 10 1 1.886503067 0.364820344 Leu343Ala 10C 295 303 1 IEDPFAEDD 30 1 1.740264997 0.423715784 Leu343Ala 10C 295 303 1 IEDPFAEDD 60 1 1.679447853 0.225339073 Leu343Ala 10C 295 303 1 IEDPFAEDD 90 1 1.658997955 0.187983398 Leu343Ala 10C 295 303 1 IEDPFAEDD 120 1 1.84709441 0.326259647 Leu343Ala 10C 295 303 1 IEDPFAEDD 180 1 1.980125256 0.253506457 Leu343Ala 10C 295 303 1 IEDPFAEDD 240 1 1.9762909 0.147163285 Leu343Ala 20C 295 303 1 IEDPFAEDD 0 1 0 0.027715501 Leu343Ala 20C 295 303 1 IEDPFAEDD 0.166666667 1 1.71875 0.39087894 Leu343Ala 20C 295 303 1 IEDPFAEDD 0.333333333 1 1.705010225 0.171715604 Leu343Ala 20C 295 303 1 IEDPFAEDD 0.5 1 1.898858214 0.251849552 Leu343Ala 20C 295 303 1 IEDPFAEDD 0.75 1 1.664855999 0.198677966 Leu343Ala 20C 295 303 1 IEDPFAEDD 1 1 1.757945637 0.233021087 Leu343Ala 20C 295 303 1 IEDPFAEDD 3 1 2.127108896 0.261640354 Leu343Ala 20C 295 303 1 IEDPFAEDD 5 1 1.954456374 0.127129798 Leu343Ala 20C 295 303 1 IEDPFAEDD 10 1 2.007817825 0.042928901 Leu343Ala 20C 295 303 1 IEDPFAEDD 30 1 2.143085378 0.177590085 Leu343Ala 20C 295 303 1 IEDPFAEDD 60 1 2.133286469 0.294175942 Leu343Ala 20C 295 303 1 IEDPFAEDD 90 1 2.267914963 0.273991827 Leu343Ala 20C 295 303 1 IEDPFAEDD 120 1 2.341193763 0.111615142 Leu343Ala 20C 295 303 1 IEDPFAEDD 180 1 2.571574642 0.25395834 Leu343Ala 20C 295 303 1 IEDPFAEDD 240 1 2.40765593 0.13029298 Leu343Ala 25C 295 303 1 IEDPFAEDD 0 1 -2.42E-13 0.01717156 Leu343Ala 25C 295 303 1 IEDPFAEDD 0.166666667 1 1.891828562 0.268719857 Leu343Ala 25C 295 303 1 IEDPFAEDD 0.333333333 1 1.653246421 0.038861952 Leu343Ala 25C 295 303 1 IEDPFAEDD 0.5 1 1.918029993 0.218108942 Leu343Ala 25C 295 303 1 IEDPFAEDD 0.75 1 1.653352931 0.244167538 Leu343Ala 25C 295 303 1 IEDPFAEDD 1 1 1.729187969 0.345088113 Leu343Ala 25C 295 303 1 IEDPFAEDD 3 1 2.050741309 0.386209481 Leu343Ala 25C 295 303 1 IEDPFAEDD 5 1 1.901627471 0.030728055 Leu343Ala 25C 295 303 1 IEDPFAEDD 10 1 1.79213531 0.304268 Leu343Ala 25C 295 303 1 IEDPFAEDD 30 1 2.067463361 0.298092263 Leu343Ala 25C 295 303 1 IEDPFAEDD 60 1 2.355146558 0.005422598 Leu343Ala 25C 295 303 1 IEDPFAEDD 90 1 2.241180982 0.297037869 Leu343Ala 25C 295 303 1 IEDPFAEDD 120 1 2.455052829 0.026811735 Leu343Ala 25C 295 303 1 IEDPFAEDD 180 1 2.27899199 0.643933514 Leu343Ala 25C 295 303 1 IEDPFAEDD 240 1 2.385395365 0.233171715 Leu343Ala 30C 295 303 1 IEDPFAEDD 0 1 0 0.009188291 Leu343Ala 30C 295 303 1 IEDPFAEDD 0.166666667 1 1.456948705 0.134359929 Leu343Ala 30C 295 303 1 IEDPFAEDD 0.333333333 1 1.403161213 0.009188291 Leu343Ala 30C 295 303 1 IEDPFAEDD 0.5 1 1.876810668 0.030125545 Leu343Ala 30C 295 303 1 IEDPFAEDD 0.75 1 1.501576346 0.089623495 Leu343Ala 30C 295 303 1 IEDPFAEDD 1 1 1.748466258 0.043531412 Leu343Ala 30C 295 303 1 IEDPFAEDD 3 1 1.704371166 0.03569877 Leu343Ala 30C 295 303 1 IEDPFAEDD 5 1 1.76497529 0.209372534 Leu343Ala 30C 295 303 1 IEDPFAEDD 10 1 1.768383606 0.041573251 Leu343Ala 30C 295 303 1 IEDPFAEDD 30 1 2.066824301 Leu343Ala 30C 295 303 1 IEDPFAEDD 60 1 1.960953476 0.068083731 Leu343Ala 30C 295 303 1 IEDPFAEDD 90 1 2.211251704 0.117489624 Leu343Ala 30C 295 303 1 IEDPFAEDD 120 1 2.079498978 0.544217962 Leu343Ala 30C 295 303 1 IEDPFAEDD 180 1 2.256944444 0.228502255 Leu343Ala 30C 295 303 1 IEDPFAEDD 240 1 2.104315781 0.269924879 Leu343Ala 40C 295 303 1 IEDPFAEDD 0 1 0 0.002711299 Leu343Ala 40C 295 303 1 IEDPFAEDD 0.166666667 1 1.685625426 0.130443608 Leu343Ala 40C 295 303 1 IEDPFAEDD 0.333333333 1 1.776584867 0.05753979 Leu343Ala 40C 295 303 1 IEDPFAEDD 0.5 1 1.823129686 0.446912453 Leu343Ala 40C 295 303 1 IEDPFAEDD 0.75 1 1.75560242 0.27248555 Leu343Ala 40C 295 303 1 IEDPFAEDD 1 1 1.774987219 0.418895697 Leu343Ala 40C 295 303 1 IEDPFAEDD 3 1 1.706820893 0.147765796 Leu343Ala 40C 295 303 1 IEDPFAEDD 5 1 1.94891786 0.050610915 Leu343Ala 40C 295 303 1 IEDPFAEDD 10 1 2.088445808 0.015966539 Leu343Ala 40C 295 303 1 IEDPFAEDD 30 1 2.330862304 0.184970843 Leu343Ala 40C 295 303 1 IEDPFAEDD 60 1 2.309240798 0.065221804 Leu343Ala 40C 295 303 1 IEDPFAEDD 90 1 2.299974438 0.147313913 Leu343Ala 40C 295 303 1 IEDPFAEDD 120 1 2.27899199 0.230912299 Leu343Ala 40C 295 303 1 IEDPFAEDD 180 1 2.585633947 Leu343Ala 40C 295 303 1 IEDPFAEDD 240 1 2.476887355 0.104385012 WT 10C 295 303 1 IEDPFAEDD 0 1 -2.42E-13 0.056636024 WT 10C 295 303 1 IEDPFAEDD 0.166666667 1 1.335527437 0.068234358 WT 10C 295 303 1 IEDPFAEDD 0.333333333 1 1.295160191 0.116284602 WT 10C 295 303 1 IEDPFAEDD 0.5 1 1.604464894 0.316920728 WT 10C 295 303 1 IEDPFAEDD 0.75 1 1.497315951 0.18587461 WT 10C 295 303 1 IEDPFAEDD 1 1 1.368119461 0.125774148 WT 10C 295 303 1 IEDPFAEDD 3 1 1.59019257 0.323247093 WT 10C 295 303 1 IEDPFAEDD 5 1 1.478357192 0.037355675 WT 10C 295 303 1 IEDPFAEDD 10 1 1.772644001 0.247481348 WT 10C 295 303 1 IEDPFAEDD 30 1 1.750702965 0.1064938 WT 10C 295 303 1 IEDPFAEDD 60 1 1.962764145 0.116585857 WT 10C 295 303 1 IEDPFAEDD 90 1 1.915473756 0.142795081 WT 10C 295 303 1 IEDPFAEDD 120 1 1.843686094 0.141891315 WT 10C 295 303 1 IEDPFAEDD 180 1 2.11368865 0.46167397 WT 10C 295 303 1 IEDPFAEDD 240 1 2.030184901 0.189339047 WT 25C 295 303 1 IEDPFAEDD 0 1 0 0.016569049 WT 25C 295 303 1 IEDPFAEDD 0.166666667 1 1.680725971 0.26058596 WT 25C 295 303 1 IEDPFAEDD 0.333333333 1 1.561115372 0.357439586 WT 25C 295 303 1 IEDPFAEDD 0.5 1 1.795969666 0.468452217 WT 25C 295 303 1 IEDPFAEDD 0.75 1 1.573790048 0.130443608 WT 25C 295 303 1 IEDPFAEDD 1 1 1.871485174 0.53969913 WT 25C 295 303 1 IEDPFAEDD 3 1 1.905355317 0.639715938 WT 25C 295 303 1 IEDPFAEDD 5 1 1.613518234 0.306527416 WT 25C 295 303 1 IEDPFAEDD 10 1 1.980977335 0.370996081 WT 25C 295 303 1 IEDPFAEDD 30 1 2.048398091 0.642577865 WT 25C 295 303 1 IEDPFAEDD 60 1 2.011545671 WT 25C 295 303 1 IEDPFAEDD 90 1 2.157251193 0.553707508 WT 25C 295 303 1 IEDPFAEDD 120 1 2.14127471 0.417841303 WT 25C 295 303 1 IEDPFAEDD 180 1 2.231808112 0.15544781 WT 25C 295 303 1 IEDPFAEDD 240 1 2.062883436 0.261489727 WT 30C 295 303 1 IEDPFAEDD 0 1 0 0.073807584 WT 30C 295 303 1 IEDPFAEDD 0.166666667 1 1.582417348 0.249288881 WT 30C 295 303 1 IEDPFAEDD 0.333333333 1 1.501469836 0.006175737 WT 30C 295 303 1 IEDPFAEDD 0.5 1 1.612453136 0.196569178 WT 30C 295 303 1 IEDPFAEDD 0.75 1 1.587529823 0.221874635 WT 30C 295 303 1 IEDPFAEDD 1 1 1.595305044 WT 30C 295 303 1 IEDPFAEDD 3 1 1.769022665 0.080887087 WT 30C 295 303 1 IEDPFAEDD 5 1 1.981935924 0.014159006 WT 30C 295 303 1 IEDPFAEDD 10 1 1.914515167 0.115531463 WT 30C 295 303 1 IEDPFAEDD 30 1 2.092280164 0.251247041 WT 30C 295 303 1 IEDPFAEDD 60 1 2.157464213 0.224435307 WT 30C 295 303 1 IEDPFAEDD 90 1 2.055214724 0.195514784 WT 30C 295 303 1 IEDPFAEDD 120 1 2.044883265 0.204100564 WT 30C 295 303 1 IEDPFAEDD 180 1 2.032954158 0.301104818 WT 30C 295 303 1 IEDPFAEDD 240 1 2.222222222 0.274142455 WT 40C 295 303 1 IEDPFAEDD 0 1 0 0.057991673 WT 40C 295 303 1 IEDPFAEDD 0.166666667 1 1.704903715 0.175330669 WT 40C 295 303 1 IEDPFAEDD 0.333333333 1 1.532783742 WT 40C 295 303 1 IEDPFAEDD 0.5 1 1.777862986 0.217657059 WT 40C 295 303 1 IEDPFAEDD 0.75 1 1.811200579 0.17593318 WT 40C 295 303 1 IEDPFAEDD 1 1 1.940929618 WT 40C 295 303 1 IEDPFAEDD 3 1 2.2155121 0.194912273 WT 40C 295 303 1 IEDPFAEDD 5 1 2.351525222 0.143246964 WT 40C 295 303 1 IEDPFAEDD 10 1 2.211038684 0.253355829 WT 40C 295 303 1 IEDPFAEDD 30 1 2.366649625 0.242962517 WT 40C 295 303 1 IEDPFAEDD 60 1 2.406910361 0.461975225 WT 40C 295 303 1 IEDPFAEDD 90 1 2.355253067 0.294929081 WT 40C 295 303 1 IEDPFAEDD 180 1 2.338957055 0.104686267 WT 40C 295 303 1 IEDPFAEDD 240 1 2.374211827 0.174728158 Leu343Ala 10C 304 310 2 WEAWSHF 0 1 1.43E-13 0.00213257 Leu343Ala 10C 304 310 2 WEAWSHF 0.166666667 1 0.751463972 0.14430388 Leu343Ala 10C 304 310 2 WEAWSHF 0.333333333 1 0.819573249 0.019548555 Leu343Ala 10C 304 310 2 WEAWSHF 0.5 1 0.927391993 0.063977089 Leu343Ala 10C 304 310 2 WEAWSHF 0.75 1 0.72859333 0.064332518 Leu343Ala 10C 304 310 2 WEAWSHF 1 1 0.856518133 0.368223693 Leu343Ala 10C 304 310 2 WEAWSHF 3 1 1.000527784 0.303180318 Leu343Ala 10C 304 310 2 WEAWSHF 5 1 0.90628063 0.213967821 Leu343Ala 10C 304 310 2 WEAWSHF 10 1 1.320968107 0.017060557 Leu343Ala 10C 304 310 2 WEAWSHF 30 1 1.47251753 0.118357615 Leu343Ala 10C 304 310 2 WEAWSHF 60 1 1.814320541 0.231383807 Leu343Ala 10C 304 310 2 WEAWSHF 90 1 2.071175451 0.386350535 Leu343Ala 10C 304 310 2 WEAWSHF 120 1 2.350649677 0.45317105 Leu343Ala 10C 304 310 2 WEAWSHF 180 1 2.73894795 0.272968915 Leu343Ala 10C 304 310 2 WEAWSHF 240 1 2.775641509 0.243823796 Leu343Ala 20C 304 310 2 WEAWSHF 0 1 1.43E-13 0.004620568 Leu343Ala 20C 304 310 2 WEAWSHF 0.166666667 1 0.725074769 Leu343Ala 20C 304 310 2 WEAWSHF 0.333333333 1 1.102063384 0.004265139 Leu343Ala 20C 304 310 2 WEAWSHF 0.5 1 1.15484179 0.389549389 Leu343Ala 20C 304 310 2 WEAWSHF 0.75 1 1.036467365 0.27190263 Leu343Ala 20C 304 310 2 WEAWSHF 1 1 1.106587248 0.218943817 Leu343Ala 20C 304 310 2 WEAWSHF 3 1 1.606474151 0.318108306 Leu343Ala 20C 304 310 2 WEAWSHF 5 1 1.572042524 0.078905077 Leu343Ala 20C 304 310 2 WEAWSHF 10 1 1.71756013 0.163852435 Leu343Ala 20C 304 310 2 WEAWSHF 30 1 2.32627108 0.269059204 Leu343Ala 20C 304 310 2 WEAWSHF 60 1 2.943024454 0.303891175 Leu343Ala 20C 304 310 2 WEAWSHF 90 1 3.085274825 0.31099974 Leu343Ala 20C 304 310 2 WEAWSHF 120 1 3.176757395 0.325216871 Leu343Ala 20C 304 310 2 WEAWSHF 180 1 3.294629169 0.282920907 Leu343Ala 20C 304 310 2 WEAWSHF 240 1 3.203397924 0.306023745 Leu343Ala 25C 304 310 2 WEAWSHF 0 1 1.43E-13 0.003909711 Leu343Ala 25C 304 310 2 WEAWSHF 0.166666667 1 1.170926638 0.271547202 Leu343Ala 25C 304 310 2 WEAWSHF 0.333333333 1 0.990474754 0.147858162 Leu343Ala 25C 304 310 2 WEAWSHF 0.5 1 1.195053909 0.236004374 Leu343Ala 25C 304 310 2 WEAWSHF 0.75 1 1.03621604 0.250932362 Leu343Ala 25C 304 310 2 WEAWSHF 1 1 1.027168313 0.253064932 Leu343Ala 25C 304 310 2 WEAWSHF 3 1 1.457689311 0.389193961 Leu343Ala 25C 304 310 2 WEAWSHF 5 1 1.449395562 0.076061651 Leu343Ala 25C 304 310 2 WEAWSHF 10 1 1.615521878 0.047982817 Leu343Ala 25C 304 310 2 WEAWSHF 30 1 2.474301943 0.010662848 Leu343Ala 25C 304 310 2 WEAWSHF 60 1 3.193847546 0.106983911 Leu343Ala 25C 304 310 2 WEAWSHF 90 1 3.089547362 0.255197501 Leu343Ala 25C 304 310 2 WEAWSHF 120 1 3.186559099 0.248088936 Leu343Ala 25C 304 310 2 WEAWSHF 180 1 3.130764784 0.467032753 Leu343Ala 25C 304 310 2 WEAWSHF 240 1 3.278544321 0.125110753 Leu343Ala 30C 304 310 2 WEAWSHF 0 1 1.43E-13 0.00213257 Leu343Ala 30C 304 310 2 WEAWSHF 0.166666667 1 0.874613587 0.088146212 Leu343Ala 30C 304 310 2 WEAWSHF 0.333333333 1 0.897986881 0.131153033 Leu343Ala 30C 304 310 2 WEAWSHF 0.5 1 1.282515268 0.21432325 Leu343Ala 30C 304 310 2 WEAWSHF 0.75 1 1.024655055 0.062199948 Leu343Ala 30C 304 310 2 WEAWSHF 1 1 1.261403906 0.33587972 Leu343Ala 30C 304 310 2 WEAWSHF 3 1 1.448390259 0.430423641 Leu343Ala 30C 304 310 2 WEAWSHF 5 1 1.848500842 0.390615674 Leu343Ala 30C 304 310 2 WEAWSHF 10 1 2.308678278 0.413718512 Leu343Ala 30C 304 310 2 WEAWSHF 30 1 3.325793561 0.020970268 Leu343Ala 30C 304 310 2 WEAWSHF 60 1 3.118449823 0.175581568 Leu343Ala 30C 304 310 2 WEAWSHF 90 1 3.135037322 0.411585942 Leu343Ala 30C 304 310 2 WEAWSHF 120 1 3.40144261 0.4449962 Leu343Ala 30C 304 310 2 WEAWSHF 180 1 3.092814597 0.649722886 Leu343Ala 30C 304 310 2 WEAWSHF 240 1 3.197366106 0.42651393 Leu343Ala 40C 304 310 2 WEAWSHF 0 1 -1.43E-13 0.017415985 Leu343Ala 40C 304 310 2 WEAWSHF 0.166666667 1 1.233255422 0.106628482 Leu343Ala 40C 304 310 2 WEAWSHF 0.333333333 1 1.328507879 0.276878626 Leu343Ala 40C 304 310 2 WEAWSHF 0.5 1 1.405413557 0.047271961 Leu343Ala 40C 304 310 2 WEAWSHF 0.75 1 1.5552037 0.044428534 Leu343Ala 40C 304 310 2 WEAWSHF 1 1 1.608736083 0.115158761 Leu343Ala 40C 304 310 2 WEAWSHF 3 1 2.159642112 0.214678678 Leu343Ala 40C 304 310 2 WEAWSHF 5 1 2.634145115 0.129375892 Leu343Ala 40C 304 310 2 WEAWSHF 10 1 3.047827289 0.187666129 Leu343Ala 40C 304 310 2 WEAWSHF 30 1 3.255422353 0.314909451 Leu343Ala 40C 304 310 2 WEAWSHF 60 1 3.232803036 0.302114034 Leu343Ala 40C 304 310 2 WEAWSHF 90 1 3.332579356 0.340144859 Leu343Ala 40C 304 310 2 WEAWSHF 120 1 3.384352459 0.419049936 Leu343Ala 40C 304 310 2 WEAWSHF 180 1 3.319761743 0.166340433 Leu343Ala 40C 304 310 2 WEAWSHF 240 1 3.420794692 0.290029472 WT 10C 304 310 2 WEAWSHF 0 1 -1.43E-13 0.064687946 WT 10C 304 310 2 WEAWSHF 0.166666667 1 0.560959059 0.109471909 WT 10C 304 310 2 WEAWSHF 0.333333333 1 0.626052427 0.168828431 WT 10C 304 310 2 WEAWSHF 0.5 1 0.83188821 0.283631763 WT 10C 304 310 2 WEAWSHF 0.75 1 0.661740682 0.217166676 WT 10C 304 310 2 WEAWSHF 1 1 0.669029129 0.131508462 WT 10C 304 310 2 WEAWSHF 3 1 0.860790671 0.357916273 WT 10C 304 310 2 WEAWSHF 5 1 0.775339918 0.067175944 WT 10C 304 310 2 WEAWSHF 10 1 0.884666616 0.324150587 WT 10C 304 310 2 WEAWSHF 30 1 1.121164141 0.000355428 WT 10C 304 310 2 WEAWSHF 60 1 1.432808063 0.017415985 WT 10C 304 310 2 WEAWSHF 90 1 1.698459373 0.09880906 WT 10C 304 310 2 WEAWSHF 120 1 1.870617507 0.103429628 WT 10C 304 310 2 WEAWSHF 180 1 2.194073739 0.453526479 WT 10C 304 310 2 WEAWSHF 240 1 2.413481113 0.013150846 WT 20C 304 310 2 WEAWSHF 0 1 0 0.04336225 WT 20C 304 310 2 WEAWSHF 0.166666667 1 0.733368519 0.334102578 WT 20C 304 310 2 WEAWSHF 0.333333333 1 0.862801277 0.153189586 WT 20C 304 310 2 WEAWSHF 0.5 1 0.787654879 0.389549389 WT 20C 304 310 2 WEAWSHF 0.75 1 0.962577597 0.031277688 WT 20C 304 310 2 WEAWSHF 1 1 0.609464927 0.200106119 WT 20C 304 310 2 WEAWSHF 3 1 1.027419639 0.494756158 WT 20C 304 310 2 WEAWSHF 5 1 1.123677398 0.578281803 WT 20C 304 310 2 WEAWSHF 10 1 1.174445198 0.210768967 WT 20C 304 310 2 WEAWSHF 30 1 1.860061826 0.063621661 WT 20C 304 310 2 WEAWSHF 60 1 2.403176757 0.064687946 WT 20C 304 310 2 WEAWSHF 90 1 2.685918219 0.001777141 WT 20C 304 310 2 WEAWSHF 120 1 2.802282038 0.373910545 WT 20C 304 310 2 WEAWSHF 180 1 2.803538666 0.256974643 WT 20C 304 310 2 WEAWSHF 240 1 3.050843198 0.227118668 WT 25C 304 310 2 WEAWSHF 0 1 0 0.062910805 WT 25C 304 310 2 WEAWSHF 0.166666667 1 0.912563774 0.382440824 WT 25C 304 310 2 WEAWSHF 0.333333333 1 0.738897685 0.27190263 WT 25C 304 310 2 WEAWSHF 0.5 1 1.104325316 0.343699142 WT 25C 304 310 2 WEAWSHF 0.75 1 0.697428938 0.031277688 WT 25C 304 310 2 WEAWSHF 1 1 0.628565684 WT 25C 304 310 2 WEAWSHF 3 1 1.232250119 0.631240616 WT 25C 304 310 2 WEAWSHF 5 1 1.3101611 WT 25C 304 310 2 WEAWSHF 10 1 1.629847445 0.375332258 WT 25C 304 310 2 WEAWSHF 30 1 2.304657066 0.463123042 WT 25C 304 310 2 WEAWSHF 60 1 2.68642087 WT 25C 304 310 2 WEAWSHF 90 1 2.85053658 0.203660401 WT 25C 304 310 2 WEAWSHF 120 1 2.922164417 0.36680198 WT 25C 304 310 2 WEAWSHF 180 1 3.267485989 0.186955273 WT 25C 304 310 2 WEAWSHF 240 1 3.018924828 0.020259412 WT 30C 304 310 2 WEAWSHF 0 1 1.43E-13 0.047271961 WT 30C 304 310 2 WEAWSHF 0.166666667 1 0.582321747 WT 30C 304 310 2 WEAWSHF 0.333333333 1 0.740154314 0.129375892 WT 30C 304 310 2 WEAWSHF 0.5 1 0.884666616 0.200106119 WT 30C 304 310 2 WEAWSHF 0.75 1 0.825605067 0.296427181 WT 30C 304 310 2 WEAWSHF 1 1 1.072909598 0.306379173 WT 30C 304 310 2 WEAWSHF 3 1 1.184246902 0.249155221 WT 30C 304 310 2 WEAWSHF 5 1 1.534343663 0.058290237 WT 30C 304 310 2 WEAWSHF 10 1 1.885445726 0.11338162 WT 30C 304 310 2 WEAWSHF 30 1 2.711553444 0.10094163 WT 30C 304 310 2 WEAWSHF 60 1 2.990273694 0.180202135 WT 30C 304 310 2 WEAWSHF 90 1 2.960617256 0.052247956 WT 30C 304 310 2 WEAWSHF 120 1 2.9415165 0.013150846 WT 30C 304 310 2 WEAWSHF 180 1 3.136293951 0.18126842 WT 30C 304 310 2 WEAWSHF 240 1 3.117947171 0.176647853 WT 40C 304 310 2 WEAWSHF 0 1 1.43E-13 0.040518823 WT 40C 304 310 2 WEAWSHF 0.166666667 1 0.943728166 0.178069566 WT 40C 304 310 2 WEAWSHF 0.333333333 1 0.685113976 WT 40C 304 310 2 WEAWSHF 0.5 1 1.124682701 0.266926634 WT 40C 304 310 2 WEAWSHF 0.75 1 1.212898037 0.188376986 WT 40C 304 310 2 WEAWSHF 1 1 1.293824926 WT 40C 304 310 2 WEAWSHF 3 1 2.158888135 0.216811248 WT 40C 304 310 2 WEAWSHF 5 1 2.509990198 0.026657121 WT 40C 304 310 2 WEAWSHF 10 1 2.938500591 0.154255871 WT 40C 304 310 2 WEAWSHF 30 1 3.241348111 0.020970268 WT 40C 304 310 2 WEAWSHF 60 1 3.136293951 0.062910805 WT 40C 304 310 2 WEAWSHF 90 1 3.27628239 0.070374798 WT 40C 304 310 2 WEAWSHF 180 1 3.526854156 0.112670763 WT 40C 304 310 2 WEAWSHF 240 1 3.329814773 0.032343973 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 0 1 1.58E-13 0.014731391 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.221944444 0.287360339 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.357916667 0.13277894 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 0.5 1 2.528333333 0.05008673 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 0.75 1 2.466527778 0.178740881 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 1 1 2.606805556 0.610861692 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 3 1 2.773888889 0.367106271 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 5 1 2.659583333 0.351982042 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 10 1 3.167361111 0.141028519 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 30 1 3.543888889 0.100762716 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 60 1 3.874861111 0.269093414 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 90 1 4.032083333 0.339804092 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 120 1 4.271388889 0.387140963 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 180 1 4.505138889 0.006678231 Leu343Ala 10C 311 323 2 FKTAGIQIVADDL 240 1 4.771805556 0.326840468 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 0 1 0 0.014142136 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.652638889 0.362392225 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.649166667 0.020034692 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 0.5 1 2.800972222 0.406782818 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 0.75 1 2.716388889 0.208203663 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 1 1 2.854305556 0.350607112 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 3 1 3.290833333 0.344518137 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 5 1 3.438611111 0.001178511 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 10 1 3.748333333 0.252201419 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 30 1 4.198611111 0.351982042 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 60 1 4.79625 0.34275037 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 90 1 4.984444444 0.331161676 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 120 1 5.184583333 0.335679303 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 180 1 5.510694444 0.217435335 Leu343Ala 20C 311 323 2 FKTAGIQIVADDL 240 1 5.476944444 0.300913219 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 0 1 0 0.001964186 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.770138889 0.208400082 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.625138889 0.026123667 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 0.5 1 2.904444444 0.272628948 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 0.75 1 2.783472222 0.125511454 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 1 1 2.889166667 0.313484006 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 3 1 3.35125 0.271254018 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 5 1 3.54 0.132386103 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 10 1 3.766666667 0.23845212 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 30 1 4.385972222 0.099191368 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 60 1 5.120138889 0.103119739 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 90 1 5.150416667 0.108619458 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 120 1 5.390972222 0.055979287 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 180 1 5.399166667 0.387337381 Leu343Ala 25C 311 323 2 FKTAGIQIVADDL 240 1 5.570972222 0.180115811 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 0 1 -1.58E-13 0.007267486 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.720277778 0.131011173 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.733333333 0.208792919 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 0.5 1 3.054305556 0.247487373 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 0.75 1 3.036666667 0.163223815 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 1 1 3.24875 0.28323555 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 3 1 3.571527778 0.328804653 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 5 1 3.778888889 0.365927759 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 10 1 4.122361111 0.406586399 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 30 1 5.129583333 0.069532167 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 60 1 5.244583333 0.161063211 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 90 1 5.493333333 0.317608796 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 120 1 5.720694444 0.502438652 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 180 1 5.541111111 0.373784501 Leu343Ala 30C 311 323 2 FKTAGIQIVADDL 240 1 5.595416667 0.279700016 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 0 1 -1.58E-13 0.024159482 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 0.166666667 1 3.113472222 0.099387786 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 0.333333333 1 3.239583333 0.328018979 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 0.5 1 3.36625 0.058139891 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 0.75 1 3.595277778 0.008053161 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 1 1 3.66125 0.068353656 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 3 1 3.957638889 0.21409622 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 5 1 4.386527778 0.1842406 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 10 1 4.802083333 0.280878527 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 30 1 5.576527778 0.246308862 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 60 1 5.765555556 0.160473956 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 90 1 5.804722222 0.340393348 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 120 1 5.866111111 0.305038009 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 180 1 5.92875 0.267522066 Leu343Ala 40C 311 323 2 FKTAGIQIVADDL 240 1 6.061527778 0.324483445 WT 10C 311 323 2 FKTAGIQIVADDL 0 1 1.58E-13 0.004910464 WT 10C 311 323 2 FKTAGIQIVADDL 0.166666667 1 1.95625 0.101351972 WT 10C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.208888889 0.092905974 WT 10C 311 323 2 FKTAGIQIVADDL 0.5 1 2.325555556 0.141617775 WT 10C 311 323 2 FKTAGIQIVADDL 0.75 1 2.416944444 0.05283659 WT 10C 311 323 2 FKTAGIQIVADDL 1 1 2.3725 0.007660323 WT 10C 311 323 2 FKTAGIQIVADDL 3 1 2.691111111 0.012767206 WT 10C 311 323 2 FKTAGIQIVADDL 5 1 2.664444444 0.184044182 WT 10C 311 323 2 FKTAGIQIVADDL 10 1 2.94375 0.117065456 WT 10C 311 323 2 FKTAGIQIVADDL 30 1 3.356527778 0.158706189 WT 10C 311 323 2 FKTAGIQIVADDL 60 1 3.902916667 0.140635682 WT 10C 311 323 2 FKTAGIQIVADDL 90 1 4.1175 0.231970308 WT 10C 311 323 2 FKTAGIQIVADDL 120 1 4.269305556 0.29266364 WT 10C 311 323 2 FKTAGIQIVADDL 180 1 4.598194444 0.019249018 WT 10C 311 323 2 FKTAGIQIVADDL 240 1 4.839027778 0.282842712 WT 20C 311 323 2 FKTAGIQIVADDL 0 1 1.58E-13 0.021606041 WT 20C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.406666667 0.004714045 WT 20C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.583472222 0.051265242 WT 20C 311 323 2 FKTAGIQIVADDL 0.5 1 2.585277778 0.058925565 WT 20C 311 323 2 FKTAGIQIVADDL 0.75 1 2.681805556 0.223327892 WT 20C 311 323 2 FKTAGIQIVADDL 1 1 2.634861111 0.078763839 WT 20C 311 323 2 FKTAGIQIVADDL 3 1 2.821388889 0.216453242 WT 20C 311 323 2 FKTAGIQIVADDL 5 1 3.031805556 0.210757105 WT 20C 311 323 2 FKTAGIQIVADDL 10 1 3.466666667 0.164991582 WT 20C 311 323 2 FKTAGIQIVADDL 30 1 4.185416667 0.284217642 WT 20C 311 323 2 FKTAGIQIVADDL 60 1 4.650138889 0.245719606 WT 20C 311 323 2 FKTAGIQIVADDL 90 1 5.119722222 0.234130912 WT 20C 311 323 2 FKTAGIQIVADDL 120 1 5.165555556 0.080531606 WT 20C 311 323 2 FKTAGIQIVADDL 180 1 5.23375 0.067371563 WT 20C 311 323 2 FKTAGIQIVADDL 240 1 5.537361111 0.035944595 WT 25C 311 323 2 FKTAGIQIVADDL 0 1 1.58E-13 0.00412479 WT 25C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.719166667 0.066193051 WT 25C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.628055556 0.07994235 WT 25C 311 323 2 FKTAGIQIVADDL 0.5 1 2.794583333 0.272236111 WT 25C 311 323 2 FKTAGIQIVADDL 0.75 1 2.716111111 0.111369318 WT 25C 311 323 2 FKTAGIQIVADDL 1 1 3.025833333 0.421710628 WT 25C 311 323 2 FKTAGIQIVADDL 3 1 3.135138889 0.370445386 WT 25C 311 323 2 FKTAGIQIVADDL 5 1 3.210555556 0.106655273 WT 25C 311 323 2 FKTAGIQIVADDL 10 1 3.800555556 0.110583644 WT 25C 311 323 2 FKTAGIQIVADDL 30 1 4.468472222 0.137885822 WT 25C 311 323 2 FKTAGIQIVADDL 60 1 4.966527778 WT 25C 311 323 2 FKTAGIQIVADDL 90 1 5.258888889 0.138475078 WT 25C 311 323 2 FKTAGIQIVADDL 120 1 5.461111111 0.08937044 WT 25C 311 323 2 FKTAGIQIVADDL 180 1 5.576944444 0.012374369 WT 25C 311 323 2 FKTAGIQIVADDL 240 1 5.674305556 0.050675986 WT 30C 311 323 2 FKTAGIQIVADDL 0 1 0 0.001767767 WT 30C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.677361111 0.033783991 WT 30C 311 323 2 FKTAGIQIVADDL 0.333333333 1 2.685 0.079549513 WT 30C 311 323 2 FKTAGIQIVADDL 0.5 1 2.814722222 0.012767206 WT 30C 311 323 2 FKTAGIQIVADDL 0.75 1 2.884444444 0.077585327 WT 30C 311 323 2 FKTAGIQIVADDL 1 1 3.235138889 0.103708995 WT 30C 311 323 2 FKTAGIQIVADDL 3 1 3.398194444 0.141028519 WT 30C 311 323 2 FKTAGIQIVADDL 5 1 3.69125 0.258093975 WT 30C 311 323 2 FKTAGIQIVADDL 10 1 4.055555556 0.221756543 WT 30C 311 323 2 FKTAGIQIVADDL 30 1 4.973611111 0.165188001 WT 30C 311 323 2 FKTAGIQIVADDL 60 1 5.434166667 0.112547829 WT 30C 311 323 2 FKTAGIQIVADDL 90 1 5.554583333 0.083281465 WT 30C 311 323 2 FKTAGIQIVADDL 120 1 5.664305556 0.206239478 WT 30C 311 323 2 FKTAGIQIVADDL 180 1 5.8675 0.151438702 WT 30C 311 323 2 FKTAGIQIVADDL 240 1 5.913333333 0.13415387 WT 40C 311 323 2 FKTAGIQIVADDL 0 1 1.58E-13 0.00274986 WT 40C 311 323 2 FKTAGIQIVADDL 0.166666667 1 2.983472222 0.125118617 WT 40C 311 323 2 FKTAGIQIVADDL 0.333333333 1 3.197083333 0.010017346 WT 40C 311 323 2 FKTAGIQIVADDL 0.5 1 3.308611111 0.008249579 WT 40C 311 323 2 FKTAGIQIVADDL 0.75 1 3.379444444 0.142992705 WT 40C 311 323 2 FKTAGIQIVADDL 1 1 3.759722222 WT 40C 311 323 2 FKTAGIQIVADDL 3 1 4.132361111 0.006088975 WT 40C 311 323 2 FKTAGIQIVADDL 5 1 4.503888889 0.143385542 WT 40C 311 323 2 FKTAGIQIVADDL 10 1 5.070416667 0.027302178 WT 40C 311 323 2 FKTAGIQIVADDL 30 1 5.816944444 0.110387225 WT 40C 311 323 2 FKTAGIQIVADDL 60 1 6.101527778 0.263790113 WT 40C 311 323 2 FKTAGIQIVADDL 90 1 6.280694444 0.288931688 WT 40C 311 323 2 FKTAGIQIVADDL 180 1 6.500694444 0.10429825 WT 40C 311 323 2 FKTAGIQIVADDL 240 1 6.356388889 0.432513648 Leu343Ala 10C 324 333 2 TVTNPKRIAT 0 1 1.51E-13 0.062364067 Leu343Ala 10C 324 333 2 TVTNPKRIAT 0.166666667 1 1.081001865 0.054827624 Leu343Ala 10C 324 333 2 TVTNPKRIAT 0.333333333 1 1.224487077 0.00866691 Leu343Ala 10C 324 333 2 TVTNPKRIAT 0.5 1 1.433253397 0.219122085 Leu343Ala 10C 324 333 2 TVTNPKRIAT 0.75 1 1.425792699 0.065378645 Leu343Ala 10C 324 333 2 TVTNPKRIAT 1 1 1.680255795 0.401127188 Leu343Ala 10C 324 333 2 TVTNPKRIAT 3 1 1.934585665 0.177483237 Leu343Ala 10C 324 333 2 TVTNPKRIAT 5 1 2.032240874 0.16749745 Leu343Ala 10C 324 333 2 TVTNPKRIAT 10 1 2.511191047 Leu343Ala 10C 324 333 2 TVTNPKRIAT 30 1 2.923661071 0.089306852 Leu343Ala 10C 324 333 2 TVTNPKRIAT 60 1 3.055688782 0.106829082 Leu343Ala 10C 324 333 2 TVTNPKRIAT 90 1 3.29616307 0.249456269 Leu343Ala 10C 324 333 2 TVTNPKRIAT 120 1 3.593791633 0.163917639 Leu343Ala 10C 324 333 2 TVTNPKRIAT 180 1 3.780309086 0.208382654 Leu343Ala 10C 324 333 2 TVTNPKRIAT 240 1 4.127364775 0.060856779 Leu343Ala 20C 324 333 2 TVTNPKRIAT 0 1 -1.51E-13 0.038247449 Leu343Ala 20C 324 333 2 TVTNPKRIAT 0.166666667 1 1.529309885 0.256992712 Leu343Ala 20C 324 333 2 TVTNPKRIAT 0.333333333 1 1.8535838 0.03843586 Leu343Ala 20C 324 333 2 TVTNPKRIAT 0.5 1 2.155075939 0.045030248 Leu343Ala 20C 324 333 2 TVTNPKRIAT 0.75 1 1.935385025 0.105887027 Leu343Ala 20C 324 333 2 TVTNPKRIAT 1 1 2.178523848 0.054450802 Leu343Ala 20C 324 333 2 TVTNPKRIAT 3 1 2.620836664 0.121901968 Leu343Ala 20C 324 333 2 TVTNPKRIAT 5 1 2.719158007 0.066509111 Leu343Ala 20C 324 333 2 TVTNPKRIAT 10 1 3.047828404 0 Leu343Ala 20C 324 333 2 TVTNPKRIAT 30 1 3.431787903 0.075364432 Leu343Ala 20C 324 333 2 TVTNPKRIAT 60 1 3.981348255 0.210831998 Leu343Ala 20C 324 333 2 TVTNPKRIAT 90 1 4.281907807 0.086857508 Leu343Ala 20C 324 333 2 TVTNPKRIAT 120 1 4.751532108 0.167685861 Leu343Ala 20C 324 333 2 TVTNPKRIAT 180 1 5.253530509 0.175222304 Leu343Ala 20C 324 333 2 TVTNPKRIAT 240 1 5.544897415 0.120017858 Leu343Ala 25C 324 333 2 TVTNPKRIAT 0 1 0 0.032783528 Leu343Ala 25C 324 333 2 TVTNPKRIAT 0.166666667 1 1.793898215 0.055958091 Leu343Ala 25C 324 333 2 TVTNPKRIAT 0.333333333 1 1.843458566 0.058219024 Leu343Ala 25C 324 333 2 TVTNPKRIAT 0.5 1 2.143884892 0.041073615 Leu343Ala 25C 324 333 2 TVTNPKRIAT 0.75 1 2.039701572 0.024870262 Leu343Ala 25C 324 333 2 TVTNPKRIAT 1 1 2.080202505 0.1989621 Leu343Ala 25C 324 333 2 TVTNPKRIAT 3 1 2.662136957 0.068958455 Leu343Ala 25C 324 333 2 TVTNPKRIAT 5 1 2.804822808 0.049175292 Leu343Ala 25C 324 333 2 TVTNPKRIAT 10 1 3.028377298 0.032971939 Leu343Ala 25C 324 333 2 TVTNPKRIAT 30 1 3.640554223 0.214411808 Leu343Ala 25C 324 333 2 TVTNPKRIAT 60 1 4.310684786 0.249456269 Leu343Ala 25C 324 333 2 TVTNPKRIAT 90 1 4.602584599 0.361184039 Leu343Ala 25C 324 333 2 TVTNPKRIAT 120 1 5.048228084 0.246441692 Leu343Ala 25C 324 333 2 TVTNPKRIAT 180 1 5.564614975 0.259253645 Leu343Ala 25C 324 333 2 TVTNPKRIAT 240 1 5.826405542 0.272630832 Leu343Ala 30C 324 333 2 TVTNPKRIAT 0 1 -1.51E-13 0.095147595 Leu343Ala 30C 324 333 2 TVTNPKRIAT 0.166666667 1 1.834665601 0.003768222 Leu343Ala 30C 324 333 2 TVTNPKRIAT 0.333333333 1 2.032907008 0.074233965 Leu343Ala 30C 324 333 2 TVTNPKRIAT 0.5 1 2.363042899 0.145453353 Leu343Ala 30C 324 333 2 TVTNPKRIAT 0.75 1 2.195710099 0.030899417 Leu343Ala 30C 324 333 2 TVTNPKRIAT 1 1 2.390354383 0.104568149 Leu343Ala 30C 324 333 2 TVTNPKRIAT 3 1 2.788435918 0.023174563 Leu343Ala 30C 324 333 2 TVTNPKRIAT 5 1 3.074340528 0.168627916 Leu343Ala 30C 324 333 2 TVTNPKRIAT 10 1 3.44217959 0.126989068 Leu343Ala 30C 324 333 2 TVTNPKRIAT 30 1 4.660138556 0 Leu343Ala 30C 324 333 2 TVTNPKRIAT 60 1 5.260724754 0.053131924 Leu343Ala 30C 324 333 2 TVTNPKRIAT 90 1 5.809485745 0.089495263 Leu343Ala 30C 324 333 2 TVTNPKRIAT 120 1 5.984545697 0.192744534 Leu343Ala 30C 324 333 2 TVTNPKRIAT 180 1 6.225819345 Leu343Ala 30C 324 333 2 TVTNPKRIAT 240 1 5.970157208 0.092133018 Leu343Ala 40C 324 333 2 TVTNPKRIAT 0 1 -1.51E-13 0.016391764 Leu343Ala 40C 324 333 2 TVTNPKRIAT 0.166666667 1 2.304956035 0.117945336 Leu343Ala 40C 324 333 2 TVTNPKRIAT 0.333333333 1 2.466826539 0.124162901 Leu343Ala 40C 324 333 2 TVTNPKRIAT 0.5 1 2.508926192 0.107205904 Leu343Ala 40C 324 333 2 TVTNPKRIAT 0.75 1 2.806554756 0.263210278 Leu343Ala 40C 324 333 2 TVTNPKRIAT 1 1 2.836530775 0.16241035 Leu343Ala 40C 324 333 2 TVTNPKRIAT 3 1 3.561550759 0.025623907 Leu343Ala 40C 324 333 2 TVTNPKRIAT 5 1 3.532640554 0.375503281 Leu343Ala 40C 324 333 2 TVTNPKRIAT 10 1 4.355981881 0.248137392 Leu343Ala 40C 324 333 2 TVTNPKRIAT 30 1 5.939515055 0.222890307 Leu343Ala 40C 324 333 2 TVTNPKRIAT 60 1 6.474420464 0.167685861 Leu343Ala 40C 324 333 2 TVTNPKRIAT 90 1 6.685718092 0.006405977 Leu343Ala 40C 324 333 2 TVTNPKRIAT 120 1 6.547695177 0.079886298 Leu343Ala 40C 324 333 2 TVTNPKRIAT 180 1 6.577005063 0.284123908 Leu343Ala 40C 324 333 2 TVTNPKRIAT 240 1 6.520783373 0.090437318 WT 10C 324 333 2 TVTNPKRIAT 0 1 0 0.022420918 WT 10C 324 333 2 TVTNPKRIAT 0.166666667 1 1.6558753 0.150728864 WT 10C 324 333 2 TVTNPKRIAT 0.333333333 1 1.316280309 0.626466839 WT 10C 324 333 2 TVTNPKRIAT 0.5 1 1.798960831 0.123597668 WT 10C 324 333 2 TVTNPKRIAT 0.75 1 2.886624034 1.454156711 WT 10C 324 333 2 TVTNPKRIAT 1 1 1.823741007 0.13791691 WT 10C 324 333 2 TVTNPKRIAT 3 1 2.138555822 0.118510569 WT 10C 324 333 2 TVTNPKRIAT 5 1 2.335331735 0.203483966 WT 10C 324 333 2 TVTNPKRIAT 10 1 2.862909672 0.107394315 WT 10C 324 333 2 TVTNPKRIAT 30 1 3.761790568 0.293732873 WT 10C 324 333 2 TVTNPKRIAT 60 1 4.693978151 0.089306852 WT 10C 324 333 2 TVTNPKRIAT 90 1 5.286171063 0.531507655 WT 10C 324 333 2 TVTNPKRIAT 120 1 5.693711697 0.323690234 WT 10C 324 333 2 TVTNPKRIAT 180 1 5.932454037 0.236644316 WT 10C 324 333 2 TVTNPKRIAT 240 1 6.021849187 0.58124818 WT 20C 324 333 2 TVTNPKRIAT 0 1 0 0.080074709 WT 20C 324 333 2 TVTNPKRIAT 0.166666667 1 1.873834266 0.027131195 WT 20C 324 333 2 TVTNPKRIAT 0.333333333 1 1.919397815 0.140177843 WT 20C 324 333 2 TVTNPKRIAT 0.5 1 2.089528377 0.052189869 WT 20C 324 333 2 TVTNPKRIAT 0.75 1 2.188915534 0.232310861 WT 20C 324 333 2 TVTNPKRIAT 1 1 2.027444711 0.191614068 WT 20C 324 333 2 TVTNPKRIAT 3 1 2.55662137 0.206310132 WT 20C 324 333 2 TVTNPKRIAT 5 1 2.922994937 0.391329812 WT 20C 324 333 2 TVTNPKRIAT 10 1 3.694111378 0.122655613 WT 20C 324 333 2 TVTNPKRIAT 30 1 4.919397815 0.304095482 WT 20C 324 333 2 TVTNPKRIAT 60 1 5.722355449 0.55336334 WT 20C 324 333 2 TVTNPKRIAT 90 1 6.195043965 0.089118441 WT 20C 324 333 2 TVTNPKRIAT 120 1 6.157207567 0.039001093 WT 20C 324 333 2 TVTNPKRIAT 180 1 6.326272315 0.354966474 WT 20C 324 333 2 TVTNPKRIAT 240 1 6.551425526 0.103249272 WT 25C 324 333 2 TVTNPKRIAT 0 1 0 0.040508382 WT 25C 324 333 2 TVTNPKRIAT 0.166666667 1 2.180522249 0.016580175 WT 25C 324 333 2 TVTNPKRIAT 0.333333333 1 2.009992006 0.130003645 WT 25C 324 333 2 TVTNPKRIAT 0.5 1 2.332001066 0.073668732 WT 25C 324 333 2 TVTNPKRIAT 0.75 1 2.539701572 WT 25C 324 333 2 TVTNPKRIAT 1 1 2.572875033 0.029203717 WT 25C 324 333 2 TVTNPKRIAT 3 1 3.110977884 0.084031341 WT 25C 324 333 2 TVTNPKRIAT 5 1 3.276845191 0.116249636 WT 25C 324 333 2 TVTNPKRIAT 10 1 4.557953637 0.190483601 WT 25C 324 333 2 TVTNPKRIAT 30 1 5.757127631 0.408098398 WT 25C 324 333 2 TVTNPKRIAT 60 1 6.217292832 WT 25C 324 333 2 TVTNPKRIAT 90 1 6.429123368 0.482709186 WT 25C 324 333 2 TVTNPKRIAT 120 1 6.476685318 0.01224672 WT 25C 324 333 2 TVTNPKRIAT 180 1 6.542632561 WT 25C 324 333 2 TVTNPKRIAT 240 1 6.610444977 0.262079812 WT 30C 324 333 2 TVTNPKRIAT 0 1 1.51E-13 0.036928572 WT 30C 324 333 2 TVTNPKRIAT 0.166666667 1 2.101385558 0.061987245 WT 30C 324 333 2 TVTNPKRIAT 0.333333333 1 2.089395151 0.186338558 WT 30C 324 333 2 TVTNPKRIAT 0.5 1 2.247668532 0.027319607 WT 30C 324 333 2 TVTNPKRIAT 0.75 1 2.475353051 0.172961371 WT 30C 324 333 2 TVTNPKRIAT 1 1 2.679589662 0.245876459 WT 30C 324 333 2 TVTNPKRIAT 3 1 3.46429523 0.23381815 WT 30C 324 333 2 TVTNPKRIAT 5 1 4.420863309 0.315211736 WT 30C 324 333 2 TVTNPKRIAT 10 1 5.34825473 0.281862975 WT 30C 324 333 2 TVTNPKRIAT 30 1 6.169997335 0.417518952 WT 30C 324 333 2 TVTNPKRIAT 60 1 6.412336797 0.352140307 WT 30C 324 333 2 TVTNPKRIAT 90 1 6.59352518 0.05407398 WT 30C 324 333 2 TVTNPKRIAT 120 1 6.75965894 0.209889943 WT 30C 324 333 2 TVTNPKRIAT 180 1 6.79243272 0.234006561 WT 30C 324 333 2 TVTNPKRIAT 240 1 6.732747136 0.065943878 WT 40C 324 333 2 TVTNPKRIAT 0 1 0 0.032595117 WT 40C 324 333 2 TVTNPKRIAT 0.166666667 1 2.490007994 0.141873543 WT 40C 324 333 2 TVTNPKRIAT 0.333333333 1 2.942845723 0.028638484 WT 40C 324 333 2 TVTNPKRIAT 0.5 1 3.412470024 0.142627187 WT 40C 324 333 2 TVTNPKRIAT 0.75 1 3.754329869 0.44333127 WT 40C 324 333 2 TVTNPKRIAT 1 1 4.167998934 WT 40C 324 333 2 TVTNPKRIAT 3 1 5.476552092 0.136409622 WT 40C 324 333 2 TVTNPKRIAT 5 1 5.879563016 0.191614068 WT 40C 324 333 2 TVTNPKRIAT 10 1 6.371969091 0.18671538 WT 40C 324 333 2 TVTNPKRIAT 30 1 6.584199307 0.3282121 WT 40C 324 333 2 TVTNPKRIAT 60 1 6.451239009 0.32293659 WT 40C 324 333 2 TVTNPKRIAT 90 1 6.646282974 0.493637028 WT 40C 324 333 2 TVTNPKRIAT 180 1 6.559285905 0.222513485 WT 40C 324 333 2 TVTNPKRIAT 240 1 6.601252331 Leu343Ala 20C 334 344 2 AIEKKAADALA 0 1 -1.81E-13 0.048034235 Leu343Ala 20C 334 344 2 AIEKKAADALA 0.166666667 1 1.032195309 Leu343Ala 20C 334 344 2 AIEKKAADALA 0.333333333 1 1.56049178 0.183792963 Leu343Ala 20C 334 344 2 AIEKKAADALA 0.5 1 1.694599034 0.051416927 Leu343Ala 20C 334 344 2 AIEKKAADALA 0.75 1 1.536731993 Leu343Ala 20C 334 344 2 AIEKKAADALA 1 1 1.641976687 0.011275642 Leu343Ala 20C 334 344 2 AIEKKAADALA 3 1 1.969032546 0.108471675 Leu343Ala 20C 334 344 2 AIEKKAADALA 5 1 1.649311923 0.167781552 Leu343Ala 20C 334 344 2 AIEKKAADALA 10 1 1.574364944 Leu343Ala 20C 334 344 2 AIEKKAADALA 30 1 1.971583933 0.036758593 Leu343Ala 20C 334 344 2 AIEKKAADALA 60 1 2.120202197 0.107569624 Leu343Ala 20C 334 344 2 AIEKKAADALA 90 1 2.569086763 0.161016167 Leu343Ala 20C 334 344 2 AIEKKAADALA 120 1 2.914002328 0.326317078 Leu343Ala 20C 334 344 2 AIEKKAADALA 180 1 3.25190158 0.105540008 Leu343Ala 20C 334 344 2 AIEKKAADALA 240 1 3.413595701 0.135307703 Leu343Ala 25C 334 344 2 AIEKKAADALA 0 1 1.81E-13 0.005186795 Leu343Ala 25C 334 344 2 AIEKKAADALA 0.166666667 1 1.586005645 0.155378346 Leu343Ala 25C 334 344 2 AIEKKAADALA 0.333333333 1 1.359251168 0.131473985 Leu343Ala 25C 334 344 2 AIEKKAADALA 0.5 1 1.659995854 0.131022959 Leu343Ala 25C 334 344 2 AIEKKAADALA 0.75 1 1.358613321 0.282567587 Leu343Ala 25C 334 344 2 AIEKKAADALA 1 1 1.320661447 0.496353758 Leu343Ala 25C 334 344 2 AIEKKAADALA 3 1 1.754556617 0.442456189 Leu343Ala 25C 334 344 2 AIEKKAADALA 5 1 1.487139417 0.092234751 Leu343Ala 25C 334 344 2 AIEKKAADALA 10 1 1.494474654 0.143651678 Leu343Ala 25C 334 344 2 AIEKKAADALA 30 1 1.935386136 0.045102568 Leu343Ala 25C 334 344 2 AIEKKAADALA 60 1 2.262282534 0.062692569 Leu343Ala 25C 334 344 2 AIEKKAADALA 90 1 2.705904865 0.208824889 Leu343Ala 25C 334 344 2 AIEKKAADALA 120 1 3.240739264 0.56242902 Leu343Ala 25C 334 344 2 AIEKKAADALA 180 1 3.365757204 0.047808722 Leu343Ala 25C 334 344 2 AIEKKAADALA 240 1 3.533191944 0.216041299 Leu343Ala 30C 334 344 2 AIEKKAADALA 0 1 0 0.009922565 Leu343Ala 30C 334 344 2 AIEKKAADALA 0.166666667 1 1.180335188 0.13981796 Leu343Ala 30C 334 344 2 AIEKKAADALA 0.333333333 1 1.24300362 0.041719875 Leu343Ala 30C 334 344 2 AIEKKAADALA 0.5 1 Leu343Ala 30C 334 344 2 AIEKKAADALA 0.75 1 1.165186331 0.218521941 Leu343Ala 30C 334 344 2 AIEKKAADALA 1 1 1.495750347 0.098323598 Leu343Ala 30C 334 344 2 AIEKKAADALA 3 1 1.404378817 0.011501155 Leu343Ala 30C 334 344 2 AIEKKAADALA 5 1 1.586005645 0.342328489 Leu343Ala 30C 334 344 2 AIEKKAADALA 10 1 1.614868205 0.022776797 Leu343Ala 30C 334 344 2 AIEKKAADALA 30 1 2.702077785 0.446289908 Leu343Ala 30C 334 344 2 AIEKKAADALA 60 1 2.84878251 0.186048092 Leu343Ala 30C 334 344 2 AIEKKAADALA 90 1 3.498907688 0.069006929 Leu343Ala 30C 334 344 2 AIEKKAADALA 120 1 3.629825708 0.293392203 Leu343Ala 30C 334 344 2 AIEKKAADALA 180 1 4.152062636 0.055025133 Leu343Ala 30C 334 344 2 AIEKKAADALA 240 1 4.310567524 0.074419237 Leu343Ala 40C 334 344 2 AIEKKAADALA 0 1 -1.81E-13 0.011050129 Leu343Ala 40C 334 344 2 AIEKKAADALA 0.166666667 1 1.624914289 0.082312186 Leu343Ala 40C 334 344 2 AIEKKAADALA 0.333333333 1 1.643411842 0.146357832 Leu343Ala 40C 334 344 2 AIEKKAADALA 0.5 1 1.522380444 0.359467465 Leu343Ala 40C 334 344 2 AIEKKAADALA 0.75 1 1.655849851 0.110275778 Leu343Ala 40C 334 344 2 AIEKKAADALA 1 1 1.403581509 0.285273741 Leu343Ala 40C 334 344 2 AIEKKAADALA 3 1 1.551083542 0.24310284 Leu343Ala 40C 334 344 2 AIEKKAADALA 5 1 1.789797643 0.102157316 Leu343Ala 40C 334 344 2 AIEKKAADALA 10 1 2.715791488 Leu343Ala 40C 334 344 2 AIEKKAADALA 30 1 3.721994546 Leu343Ala 40C 334 344 2 AIEKKAADALA 60 1 4.417566296 0.343681566 Leu343Ala 40C 334 344 2 AIEKKAADALA 90 1 4.443399085 0.124032061 Leu343Ala 40C 334 344 2 AIEKKAADALA 120 1 4.66329671 0.315041436 Leu343Ala 40C 334 344 2 AIEKKAADALA 180 1 4.808406819 0.158986551 Leu343Ala 40C 334 344 2 AIEKKAADALA 240 1 4.958779161 WT 10C 334 344 2 AIEKKAADALL 0 1 0 0.065398723 WT 10C 334 344 2 AIEKKAADALL 0.166666667 1 1.282550111 0.312786307 WT 10C 334 344 2 AIEKKAADALL 0.333333333 1 1.001259747 0.004284744 WT 10C 334 344 2 AIEKKAADALL 0.5 1 1.392897578 0.025933976 WT 10C 334 344 2 AIEKKAADALL 0.75 1 1.207603132 0.287077844 WT 10C 334 344 2 AIEKKAADALL 1 1 1.145572547 0.535818505 WT 10C 334 344 2 AIEKKAADALL 3 1 1.309020746 0.062918082 WT 10C 334 344 2 AIEKKAADALL 5 1 1.256717322 0.479214782 WT 10C 334 344 2 AIEKKAADALL 10 1 1.519031749 0.090205135 WT 10C 334 344 2 AIEKKAADALL 30 1 1.696512574 0.828759682 WT 10C 334 344 2 AIEKKAADALL 60 1 2.079380013 0.514169272 WT 10C 334 344 2 AIEKKAADALL 90 1 2.023090048 0.520258119 WT 10C 334 344 2 AIEKKAADALL 120 1 2.184146322 0.410658879 WT 10C 334 344 2 AIEKKAADALL 180 1 2.857074516 0.083665263 WT 10C 334 344 2 AIEKKAADALL 240 1 2.949721739 0.310756692 WT 20C 334 344 2 AIEKKAADALL 0 1 0 0.00157859 WT 20C 334 344 2 AIEKKAADALL 0.166666667 1 1.160880866 0.033150387 WT 20C 334 344 2 AIEKKAADALL 0.333333333 1 1.342826617 0.285499254 WT 20C 334 344 2 AIEKKAADALL 0.5 1 1.35941063 0.029767695 WT 20C 334 344 2 AIEKKAADALL 0.75 1 1.556983623 0.337141694 WT 20C 334 344 2 AIEKKAADALL 1 1 1.387794805 WT 20C 334 344 2 AIEKKAADALL 3 1 1.506753201 0.234082327 WT 20C 334 344 2 AIEKKAADALL 5 1 1.272344565 0.32654259 WT 20C 334 344 2 AIEKKAADALL 10 1 1.778635327 0.34480913 WT 20C 334 344 2 AIEKKAADALL 30 1 1.978759707 0.135307703 WT 20C 334 344 2 AIEKKAADALL 60 1 2.883545152 0.848379299 WT 20C 334 344 2 AIEKKAADALL 90 1 3.535264945 0.00473577 WT 20C 334 344 2 AIEKKAADALL 120 1 3.237390569 0.032699362 WT 20C 334 344 2 AIEKKAADALL 180 1 3.423322862 0.012403206 WT 20C 334 344 2 AIEKKAADALL 240 1 4.012374225 0.017364489 WT 25C 334 344 2 AIEKKAADALL 0 1 0 0.022325771 WT 25C 334 344 2 AIEKKAADALL 0.166666667 1 1.537210378 0.346162207 WT 25C 334 344 2 AIEKKAADALL 0.333333333 1 1.320342524 0.08186116 WT 25C 334 344 2 AIEKKAADALL 0.5 1 1.746424072 0.011501155 WT 25C 334 344 2 AIEKKAADALL 0.75 1 1.702253193 WT 25C 334 344 2 AIEKKAADALL 1 1 1.782940792 0.48936286 WT 25C 334 344 2 AIEKKAADALL 3 1 1.915453429 0.229797583 WT 25C 334 344 2 AIEKKAADALL 5 1 1.552040312 0.013079745 WT 25C 334 344 2 AIEKKAADALL 10 1 2.177130009 0.035631029 WT 25C 334 344 2 AIEKKAADALL 30 1 3.126086332 0.043523978 WT 25C 334 344 2 AIEKKAADALL 60 1 3.380427676 WT 25C 334 344 2 AIEKKAADALL 90 1 3.922118926 0.137337319 WT 25C 334 344 2 AIEKKAADALL 120 1 4.227488 0.096519495 WT 25C 334 344 2 AIEKKAADALL 180 1 3.990209054 WT 25C 334 344 2 AIEKKAADALL 240 1 4.376106265 0.001353077 WT 30C 334 344 2 AIEKKAADALL 0 1 1.81E-13 0.062692569 WT 30C 334 344 2 AIEKKAADALL 0.166666667 1 1.339318461 0.123355523 WT 30C 334 344 2 AIEKKAADALL 0.333333333 1 1.476614948 0.458242088 WT 30C 334 344 2 AIEKKAADALL 0.5 1 1.414584363 0.016462437 WT 30C 334 344 2 AIEKKAADALL 0.75 1 1.615027667 0.176802065 WT 30C 334 344 2 AIEKKAADALL 1 1 1.426065603 0.352025541 WT 30C 334 344 2 AIEKKAADALL 3 1 1.755672848 0.583627226 WT 30C 334 344 2 AIEKKAADALL 5 1 2.134394285 0.451702216 WT 30C 334 344 2 AIEKKAADALL 10 1 2.398303328 0.201608478 WT 30C 334 344 2 AIEKKAADALL 30 1 3.274226212 0.116139112 WT 30C 334 344 2 AIEKKAADALL 60 1 3.809538996 0.11681565 WT 30C 334 344 2 AIEKKAADALL 90 1 4.044904403 0.167781552 WT 30C 334 344 2 AIEKKAADALL 120 1 4.382803655 0.137788344 WT 30C 334 344 2 AIEKKAADALL 180 1 4.349954553 0.072389621 WT 30C 334 344 2 AIEKKAADALL 240 1 4.572563027 0.109373727 WT 40C 334 344 2 AIEKKAADALL 0 1 1.81E-13 0.011050129 WT 40C 334 344 2 AIEKKAADALL 0.166666667 1 1.55953501 0.296098357 WT 40C 334 344 2 AIEKKAADALL 0.333333333 1 1.818660203 0.059986415 WT 40C 334 344 2 AIEKKAADALL 0.5 1 1.592543573 0.020296155 WT 40C 334 344 2 AIEKKAADALL 0.75 1 1.66446078 0.101255265 WT 40C 334 344 2 AIEKKAADALL 1 1 2.438966051 WT 40C 334 344 2 AIEKKAADALL 3 1 2.882588382 0.017138976 WT 40C 334 344 2 AIEKKAADALL 5 1 3.244885267 0.102382829 WT 40C 334 344 2 AIEKKAADALL 10 1 4.029596084 0.245132456 WT 40C 334 344 2 AIEKKAADALL 30 1 4.698218813 0.284597202 WT 40C 334 344 2 AIEKKAADALL 60 1 5.114732662 0.090656161 WT 40C 334 344 2 AIEKKAADALL 90 1 5.183141713 0.128767831 WT 40C 334 344 2 AIEKKAADALL 180 1 5.357752228 0.235435404 WT 40C 334 344 2 AIEKKAADALL 240 1 5.386933712 0.310080153 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 2.52E-13 0.0166319 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.458616252 0.286508007 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.450627968 0.610359335 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 2.923268096 0.89780877 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 2.287090046 0.380964644 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 2.666311632 0.601572671 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.006035592 0.447492242 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 2.31571473 0.070607121 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 3.288288288 0.719878825 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 3.576975991 1.222287717 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 3.543691475 0.079393785 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 3.507744197 0.319144187 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 3.871211113 0.418935585 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 3.858784893 0.298432765 Leu343Ala 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 4.318333111 0.260461824 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.022280469 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.921714818 0.238495164 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 3.160475747 0.74435596 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.260329295 0.187030418 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 2.874450805 0.150942334 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.17334576 0.378454169 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 4.111969112 0.313809427 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.810633293 0.906909243 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 4.091998402 0.737452153 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.244885279 0.233788023 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.239559757 0.018514756 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.493631563 0.407324636 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.88239471 0.731803583 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 5.246527315 0.440902245 Leu343Ala 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 5.075223006 0.485463183 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.018828566 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 3.238139617 0.216842314 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.767052767 0.199582795 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.339102649 0.306278 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 2.87001287 0.152511381 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 2.901300315 0.000941428 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.795100519 0.122071867 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.434518262 0.384730357 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 3.50619092 0.073745215 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.197843163 0.177616136 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.463009808 0.308474666 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.29503395 0.139331385 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.354502286 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 4.713531265 0.293098005 Leu343Ala 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 4.679359162 0.055230459 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 -2.52E-13 0.050837127 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.680069232 0.562346493 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.697599077 0.463496523 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 2.877113567 0.55293221 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.118093463 0.241633259 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.322682288 0.066527598 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.665956597 0.118306154 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 3.824168997 0.347073226 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.026538854 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.247769937 0.642367896 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.725513691 0.481383661 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.516265034 0.226256597 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 4.881507123 0.479814613 Leu343Ala 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 4.683131407 0.664334556 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 2.52E-13 0.069979502 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 3.404340301 0.49801556 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 3.472684507 0.36119465 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.459592598 1.162350117 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 3.627346558 0.829084505 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.623352416 0.955235895 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.909377358 0.539438405 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 4.251542183 0.275210867 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 4.765898904 0.754711671 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 5.1582124 1.252413422 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 5.085874051 0.838812598 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.857320375 0.113912822 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.638086362 0.215587076 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 5.294013225 0.766008811 Leu343Ala 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 5.183952425 0.324165138 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.00564857 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.55225669 0.175733279 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.402920162 0.353663224 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.059068921 0.48703223 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 2.814538677 0.178243754 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 2.539386677 0.08221807 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.131407269 0.610673144 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.011361115 0.029811896 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 3.514179204 0.810569749 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 3.737407358 0.245712781 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.341854169 0.079393785 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.128833267 0.188599465 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.141037589 0.107950443 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 4.90303111 0.962139702 WT 10C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 4.57085164 0.315378474 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.019456184 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.677184574 0.737138343 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 3.131851063 0.285566578 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.016242844 1.020194446 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 3.12519416 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 2.495007323 0.161925664 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.682376958 1.095508709 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.51240403 1.226681049 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 4.137265344 0.226884216 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.35694315 0.130230912 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.816491368 0.621970284 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 5.337948786 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 4.623441175 0.678455981 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 4.664048285 0.367470839 WT 20C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 4.973594284 0.713602636 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.000313809 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 3.594949629 0.815904509 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.905738251 0.418621775 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.086806018 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 3.193316469 0.537241739 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.819287268 1.374799098 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 4.331646918 1.361305293 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 3.703013358 0.636719327 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 4.587715795 0.968415891 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.516708827 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 5.260950606 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.432831847 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 5.414281276 0.526886027 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 4.855767097 0.138703767 WT 25C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 5.47042116 1.057537768 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.002510475 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 2.633914703 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 3.019349399 0.214331838 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.23725203 0.457847954 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 3.367061643 0.815276891 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.586739449 0.466320808 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 3.695246971 0.164749949 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 4.37824524 0.189854703 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 4.493631563 0.229394691 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 4.353170905 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 4.739715085 0.217783742 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 4.999778103 0.54163507 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 120 1 5.052367639 0.117050916 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 5.296232193 0.434312247 WT 30C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 5.568721431 0.500839845 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0 1 0 0.01098333 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.166666667 1 3.400568056 0.571133157 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.333333333 1 2.749966715 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.5 1 3.768472906 0.842264501 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 0.75 1 3.715661474 0.745611198 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 1 1 3.758265655 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 3 1 5.291350464 0.841950692 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 5 1 5.487507212 0.326675613 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 10 1 5.416278347 0.27929039 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 30 1 5.637287534 0.743100722 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 60 1 5.556517108 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 90 1 6.479607687 0.661510271 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 180 1 6.594550215 0.001882857 WT 40C 345 364 3 LKVNQIGTLSESIKAAQDSF 240 1 7.148182665 0.456592716 Leu343Ala 10C 365 371 1 AAGWGVM 0 1 -8.02E-14 0.000498822 Leu343Ala 10C 365 371 1 AAGWGVM 0.166666667 1 0.440829767 0.048285946 Leu343Ala 10C 365 371 1 AAGWGVM 0.333333333 1 0.453386608 0.125703084 Leu343Ala 10C 365 371 1 AAGWGVM 0.5 1 0.562588797 0.136677162 Leu343Ala 10C 365 371 1 AAGWGVM 0.75 1 0.465802361 0.037311868 Leu343Ala 10C 365 371 1 AAGWGVM 1 1 0.621281447 0.219481574 Leu343Ala 10C 365 371 1 AAGWGVM 3 1 0.786848332 0.117422642 Leu343Ala 10C 365 371 1 AAGWGVM 5 1 0.777607061 0.067440702 Leu343Ala 10C 365 371 1 AAGWGVM 10 1 1.12665902 0.135879047 Leu343Ala 10C 365 371 1 AAGWGVM 30 1 1.165599335 0.141864909 Leu343Ala 10C 365 371 1 AAGWGVM 60 1 1.178438352 0.015962296 Leu343Ala 10C 365 371 1 AAGWGVM 90 1 1.172230476 0.059060496 Leu343Ala 10C 365 371 1 AAGWGVM 120 1 1.277834918 0.114230183 Leu343Ala 10C 365 371 1 AAGWGVM 180 1 1.409611204 0.028033783 Leu343Ala 10C 365 371 1 AAGWGVM 240 1 1.476769139 0.022646508 Leu343Ala 20C 365 371 1 AAGWGVM 0 1 0 0.014565595 Leu343Ala 20C 365 371 1 AAGWGVM 0.166666667 1 0.505800837 Leu343Ala 20C 365 371 1 AAGWGVM 0.333333333 1 0.737749673 0.036513753 Leu343Ala 20C 365 371 1 AAGWGVM 0.5 1 0.843142483 0.158026734 Leu343Ala 20C 365 371 1 AAGWGVM 0.75 1 0.795384162 0.127997664 Leu343Ala 20C 365 371 1 AAGWGVM 1 1 0.876227643 0.074124914 Leu343Ala 20C 365 371 1 AAGWGVM 3 1 1.265630798 0.170896335 Leu343Ala 20C 365 371 1 AAGWGVM 5 1 1.204610195 0.008978792 Leu343Ala 20C 365 371 1 AAGWGVM 10 1 1.284677691 0.043197964 Leu343Ala 20C 365 371 1 AAGWGVM 30 1 1.356914798 0.072329155 Leu343Ala 20C 365 371 1 AAGWGVM 60 1 1.408341411 0.107346443 Leu343Ala 20C 365 371 1 AAGWGVM 90 1 1.508866682 0.009477613 Leu343Ala 20C 365 371 1 AAGWGVM 120 1 1.663499239 0.008280441 Leu343Ala 20C 365 371 1 AAGWGVM 180 1 1.836755425 0.009677142 Leu343Ala 20C 365 371 1 AAGWGVM 240 1 1.899398541 0.003292224 Leu343Ala 25C 365 371 1 AAGWGVM 0 1 8.02E-14 0.019653577 Leu343Ala 25C 365 371 1 AAGWGVM 0.166666667 1 0.774009315 0.119018872 Leu343Ala 25C 365 371 1 AAGWGVM 0.333333333 1 0.686675781 0.050979584 Leu343Ala 25C 365 371 1 AAGWGVM 0.5 1 0.890759717 0.098766708 Leu343Ala 25C 365 371 1 AAGWGVM 0.75 1 0.835523726 0.089089566 Leu343Ala 25C 365 371 1 AAGWGVM 1 1 0.843989012 0.130990594 Leu343Ala 25C 365 371 1 AAGWGVM 3 1 1.292084817 0.188454861 Leu343Ala 25C 365 371 1 AAGWGVM 5 1 1.202564417 0.072229391 Leu343Ala 25C 365 371 1 AAGWGVM 10 1 1.224503617 0.097070714 Leu343Ala 25C 365 371 1 AAGWGVM 30 1 1.378924542 0.089388859 Leu343Ala 25C 365 371 1 AAGWGVM 60 1 1.517825776 0.001695994 Leu343Ala 25C 365 371 1 AAGWGVM 90 1 1.553732697 0.027934019 Leu343Ala 25C 365 371 1 AAGWGVM 120 1 1.800354695 0.169000812 Leu343Ala 25C 365 371 1 AAGWGVM 180 1 1.828854492 0.057065209 Leu343Ala 25C 365 371 1 AAGWGVM 240 1 1.95110733 0.038209747 Leu343Ala 30C 365 371 1 AAGWGVM 0 1 8.02E-14 0.00847997 Leu343Ala 30C 365 371 1 AAGWGVM 0.166666667 1 0.680538449 0.038109982 Leu343Ala 30C 365 371 1 AAGWGVM 0.333333333 1 0.783109497 0.011572665 Leu343Ala 30C 365 371 1 AAGWGVM 0.5 1 1.003559653 0.042998436 Leu343Ala 30C 365 371 1 AAGWGVM 0.75 1 0.92617283 0.023943444 Leu343Ala 30C 365 371 1 AAGWGVM 1 1 1.050259814 0.095474485 Leu343Ala 30C 365 371 1 AAGWGVM 3 1 1.130962207 0.016062061 Leu343Ala 30C 365 371 1 AAGWGVM 5 1 1.185492757 0.080609596 Leu343Ala 30C 365 371 1 AAGWGVM 10 1 1.207149781 0.017458762 Leu343Ala 30C 365 371 1 AAGWGVM 30 1 1.362417234 Leu343Ala 30C 365 371 1 AAGWGVM 60 1 1.556131195 0.071032219 Leu343Ala 30C 365 371 1 AAGWGVM 90 1 1.775664278 0.022347215 Leu343Ala 30C 365 371 1 AAGWGVM 120 1 1.901938127 0.116325234 Leu343Ala 30C 365 371 1 AAGWGVM 180 1 2.049939544 0.023544387 Leu343Ala 30C 365 371 1 AAGWGVM 240 1 2.042461874 0.021748629 Leu343Ala 40C 365 371 1 AAGWGVM 0 1 0 0.003391988 Leu343Ala 40C 365 371 1 AAGWGVM 0.166666667 1 0.88610381 0.068338581 Leu343Ala 40C 365 371 1 AAGWGVM 0.333333333 1 0.954249362 0.027036139 Leu343Ala 40C 365 371 1 AAGWGVM 0.5 1 1.04235888 0.120714866 Leu343Ala 40C 365 371 1 AAGWGVM 0.75 1 1.10140425 0.081707004 Leu343Ala 40C 365 371 1 AAGWGVM 1 1 1.130115678 0.143461138 Leu343Ala 40C 365 371 1 AAGWGVM 3 1 1.160661252 0.035017288 Leu343Ala 40C 365 371 1 AAGWGVM 5 1 1.215191802 0.003391988 Leu343Ala 40C 365 371 1 AAGWGVM 10 1 1.385061874 0.085797343 Leu343Ala 40C 365 371 1 AAGWGVM 30 1 1.702086834 0.150045585 Leu343Ala 40C 365 371 1 AAGWGVM 60 1 1.977208628 0.164012595 Leu343Ala 40C 365 371 1 AAGWGVM 90 1 2.080061853 0.011772194 Leu343Ala 40C 365 371 1 AAGWGVM 120 1 2.072302008 0.013767481 Leu343Ala 40C 365 371 1 AAGWGVM 180 1 2.140659192 0.067141409 Leu343Ala 40C 365 371 1 AAGWGVM 240 1 2.142987145 0.017957583 WT 10C 365 371 1 AAGWGVM 0 1 -8.02E-14 0.003092695 WT 10C 365 371 1 AAGWGVM 0.166666667 1 0.437232021 0.03461823 WT 10C 365 371 1 AAGWGVM 0.333333333 1 0.421782874 0.088989802 WT 10C 365 371 1 AAGWGVM 0.5 1 0.586785406 0.098267887 WT 10C 365 371 1 AAGWGVM 0.75 1 0.542060479 0.051977227 WT 10C 365 371 1 AAGWGVM 1 1 0.474620367 0.035815402 WT 10C 365 371 1 AAGWGVM 3 1 0.756373302 0.102657518 WT 10C 365 371 1 AAGWGVM 5 1 0.796512867 0.011373136 WT 10C 365 371 1 AAGWGVM 10 1 0.968922524 0.128696014 WT 10C 365 371 1 AAGWGVM 30 1 1.018444447 0.014565595 WT 10C 365 371 1 AAGWGVM 60 1 1.082639533 0.010575021 WT 10C 365 371 1 AAGWGVM 90 1 1.025075588 0.041102913 WT 10C 365 371 1 AAGWGVM 120 1 1.063945359 0.023444623 WT 10C 365 371 1 AAGWGVM 180 1 1.174487885 0.228260837 WT 10C 365 371 1 AAGWGVM 240 1 1.144647752 0.12859625 WT 20C 365 371 1 AAGWGVM 0 1 -8.02E-14 0.007183033 WT 20C 365 371 1 AAGWGVM 0.166666667 1 0.520615088 0.153437573 WT 20C 365 371 1 AAGWGVM 0.333333333 1 0.629887821 0.052974871 WT 20C 365 371 1 AAGWGVM 0.5 1 0.624597017 0.205315036 WT 20C 365 371 1 AAGWGVM 0.75 1 0.686111429 WT 20C 365 371 1 AAGWGVM 1 1 0.580013178 0.087393572 WT 20C 365 371 1 AAGWGVM 3 1 0.785860715 WT 20C 365 371 1 AAGWGVM 5 1 0.811679837 WT 20C 365 371 1 AAGWGVM 10 1 1.048637301 0.032423414 WT 20C 365 371 1 AAGWGVM 30 1 1.051600151 0.065744708 WT 20C 365 371 1 AAGWGVM 60 1 1.224150897 0.181870414 WT 20C 365 371 1 AAGWGVM 90 1 1.361852882 0.062552249 WT 20C 365 371 1 AAGWGVM 120 1 1.178791073 0.170996099 WT 20C 365 371 1 AAGWGVM 180 1 1.237201546 0.092182261 WT 20C 365 371 1 AAGWGVM 240 1 1.348026248 0.173689737 WT 25C 365 371 1 AAGWGVM 0 1 0 0.023644151 WT 25C 365 371 1 AAGWGVM 0.166666667 1 0.746073871 0.185860988 WT 25C 365 371 1 AAGWGVM 0.333333333 1 0.615920099 0.075820908 WT 25C 365 371 1 AAGWGVM 0.5 1 0.86677474 0.21868346 WT 25C 365 371 1 AAGWGVM 0.75 1 0.778312502 0.088989802 WT 25C 365 371 1 AAGWGVM 1 1 0.965818586 0.229058952 WT 25C 365 371 1 AAGWGVM 3 1 1.134983218 0.257591557 WT 25C 365 371 1 AAGWGVM 5 1 1.02267709 0.101959168 WT 25C 365 371 1 AAGWGVM 10 1 1.173711901 0.154934039 WT 25C 365 371 1 AAGWGVM 30 1 1.268311472 0.214094299 WT 25C 365 371 1 AAGWGVM 60 1 1.274096084 WT 25C 365 371 1 AAGWGVM 90 1 1.389506149 0.20930561 WT 25C 365 371 1 AAGWGVM 120 1 1.417159417 0.111736074 WT 25C 365 371 1 AAGWGVM 180 1 1.670694732 0.391874375 WT 25C 365 371 1 AAGWGVM 240 1 1.56353832 0.191846849 WT 30C 365 371 1 AAGWGVM 0 1 0 0.012769837 WT 30C 365 371 1 AAGWGVM 0.166666667 1 0.658669793 0.143161845 WT 30C 365 371 1 AAGWGVM 0.333333333 1 0.681455521 0.014366067 WT 30C 365 371 1 AAGWGVM 0.5 1 0.743675373 0.10475257 WT 30C 365 371 1 AAGWGVM 0.75 1 0.838980385 0.125603319 WT 30C 365 371 1 AAGWGVM 1 1 0.932451251 0.079611953 WT 30C 365 371 1 AAGWGVM 3 1 1.00489999 0.028033783 WT 30C 365 371 1 AAGWGVM 5 1 1.101333706 0.065644944 WT 30C 365 371 1 AAGWGVM 10 1 1.069659427 0.035216816 WT 30C 365 371 1 AAGWGVM 30 1 1.202846593 0.191846849 WT 30C 365 371 1 AAGWGVM 60 1 1.221470223 0.041202677 WT 30C 365 371 1 AAGWGVM 90 1 1.313177487 0.096871186 WT 30C 365 371 1 AAGWGVM 120 1 1.377654749 0.054970158 WT 30C 365 371 1 AAGWGVM 180 1 1.51267606 0.119816987 WT 30C 365 371 1 AAGWGVM 240 1 1.667802426 0.095574249 WT 40C 365 371 1 AAGWGVM 0 1 -8.02E-14 0.015064417 WT 40C 365 371 1 AAGWGVM 0.166666667 1 0.757995815 0.171794214 WT 40C 365 371 1 AAGWGVM 0.333333333 1 0.697327932 WT 40C 365 371 1 AAGWGVM 0.5 1 0.961585943 0.164611181 WT 40C 365 371 1 AAGWGVM 0.75 1 0.998198305 0.159722728 WT 40C 365 371 1 AAGWGVM 1 1 1.018444447 WT 40C 365 371 1 AAGWGVM 3 1 1.24947621 0.160520842 WT 40C 365 371 1 AAGWGVM 5 1 1.247783153 0.067739995 WT 40C 365 371 1 AAGWGVM 10 1 1.246866081 0.111935603 WT 40C 365 371 1 AAGWGVM 30 1 1.408341411 0.178677954 WT 40C 365 371 1 AAGWGVM 60 1 1.402909519 WT 40C 365 371 1 AAGWGVM 90 1 1.793370835 0.15932367 WT 40C 365 371 1 AAGWGVM 180 1 1.9087809 0.082704648 WT 40C 365 371 1 AAGWGVM 240 1 2.076957915 0.144159489 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 0 1 -3.39E-13 0.050659606 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 0.166666667 1 4.269057172 0.092453781 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 0.333333333 1 4.261594307 0.342796668 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 0.5 1 4.582796007 0.576675182 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 0.75 1 4.282788843 0.258786154 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 1 1 4.60637866 0.456358618 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 3 1 4.935043225 0.116517094 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 5 1 4.538018819 0.271873219 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 10 1 5.651776759 0.475778133 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 30 1 5.98730716 0.577941672 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 60 1 6.366122177 0.117361421 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 90 1 6.456870612 0.025329803 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 120 1 6.786132207 0.013087065 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 180 1 6.944046426 0.227968227 Leu343Ala 10C 372 383 2 VSHRSGETEDTF 240 1 6.893298945 0.230501208 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 0 1 -3.39E-13 0.024485476 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 0.166666667 1 4.752352295 0.188284869 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 0.333333333 1 4.665484549 0.725698857 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 0.5 1 5.127883651 0.097097578 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 0.75 1 4.860414577 0.168865354 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 1 1 5.151764819 0.016886535 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 3 1 5.829691456 0.106807336 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 5 1 5.821333047 0.391345457 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 10 1 6.297165306 0.145646367 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 30 1 6.799863878 0.198838954 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 60 1 6.866731146 0.074722919 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 90 1 6.969121651 0.325910132 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 120 1 7.077482448 0.33308691 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 180 1 7.263457038 0.286226774 Leu343Ala 20C 372 383 2 VSHRSGETEDTF 240 1 7.175992263 0.290026245 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 0 1 3.39E-13 0.043904992 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.014448107 0.188284869 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 0.333333333 1 4.677126618 0.748073516 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 0.5 1 5.238035536 0.258786154 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 0.75 1 4.999522377 0.371503778 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 1 1 5.175048957 0.055725567 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 3 1 5.868796867 0.168021027 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 5 1 5.841930554 0.426385018 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 10 1 6.315673211 0.177730785 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 30 1 6.889418255 0.331398256 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 60 1 7.184350672 0.529815047 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 90 1 6.92613555 0.271028892 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 120 1 7.222859054 0.544590765 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 180 1 7.048825047 0.011398411 Leu343Ala 25C 372 383 2 VSHRSGETEDTF 240 1 7.278382768 0.433139632 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 0 1 3.39E-13 0.016464372 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 0.166666667 1 4.822503224 0.359683203 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 0.333333333 1 4.949371926 0.25203154 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 0.5 1 5.528490233 0.132981466 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 0.75 1 5.344008216 0.205593568 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 1 1 5.612074319 0.025751966 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 3 1 6.072980848 0.070501285 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 5 1 6.466124565 0.073456429 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 10 1 6.712100588 0.022796823 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 30 1 6.720160482 0.091609454 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 60 1 6.806431199 0.646754304 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 90 1 7.238381813 0.228390391 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 120 1 7.478984573 0.1372031 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 180 1 7.227038258 0.25203154 Leu343Ala 30C 372 383 2 VSHRSGETEDTF 240 1 7.397788605 0.08738782 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 0 1 3.39E-13 0.016464372 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.456548217 0.433139632 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 0.333333333 1 5.62908965 0.006754614 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 0.5 1 5.735360845 0.547123745 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 0.75 1 6.153579788 0.598205515 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 1 1 6.047010078 0.461846742 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 3 1 6.691204567 0.239788802 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 5 1 6.807625257 0.113984114 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 10 1 6.952703349 0.149445838 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 30 1 7.333010938 0.315778211 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 60 1 7.622271577 0.392189784 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 90 1 7.778096194 0.022374659 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 120 1 7.816604576 0.142691224 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 180 1 7.792723409 0.215303326 Leu343Ala 40C 372 383 2 VSHRSGETEDTF 240 1 7.975414339 0.033773071 WT 10C 372 383 2 VSHRSGETEDTF 0 1 0 0.002955144 WT 10C 372 383 2 VSHRSGETEDTF 0.166666667 1 4.729366672 0.119894401 WT 10C 372 383 2 VSHRSGETEDTF 0.333333333 1 4.888773464 0.100474885 WT 10C 372 383 2 VSHRSGETEDTF 0.5 1 5.024597603 0.366015654 WT 10C 372 383 2 VSHRSGETEDTF 0.75 1 5.232662273 0.130026322 WT 10C 372 383 2 VSHRSGETEDTF 1 1 4.816532933 0.300580329 WT 10C 372 383 2 VSHRSGETEDTF 3 1 5.222512777 0.125804688 WT 10C 372 383 2 VSHRSGETEDTF 5 1 5.295648852 0.075567246 WT 10C 372 383 2 VSHRSGETEDTF 10 1 5.703419784 0.240210965 WT 10C 372 383 2 VSHRSGETEDTF 30 1 6.123131299 0.1372031 WT 10C 372 383 2 VSHRSGETEDTF 60 1 6.842551465 0.202216261 WT 10C 372 383 2 VSHRSGETEDTF 90 1 6.795386159 0.195461647 WT 10C 372 383 2 VSHRSGETEDTF 120 1 6.709413957 0.023218986 WT 10C 372 383 2 VSHRSGETEDTF 180 1 7.17121603 0.127493342 WT 10C 372 383 2 VSHRSGETEDTF 240 1 6.889418255 0.205171405 WT 20C 372 383 2 VSHRSGETEDTF 0 1 -3.39E-13 0.007176778 WT 20C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.014149592 0.33182042 WT 20C 372 383 2 VSHRSGETEDTF 0.333333333 1 5.162212829 0.136780936 WT 20C 372 383 2 VSHRSGETEDTF 0.5 1 5.12907771 0.479999767 WT 20C 372 383 2 VSHRSGETEDTF 0.75 1 5.390277977 0.19335083 WT 20C 372 383 2 VSHRSGETEDTF 1 1 5.099524765 0.28200514 WT 20C 372 383 2 VSHRSGETEDTF 3 1 5.594760472 0.541635621 WT 20C 372 383 2 VSHRSGETEDTF 5 1 5.719838086 0.534458844 WT 20C 372 383 2 VSHRSGETEDTF 10 1 6.438362708 0.288337591 WT 20C 372 383 2 VSHRSGETEDTF 30 1 7.030018628 0.191240013 WT 20C 372 383 2 VSHRSGETEDTF 60 1 7.013301811 0.059525037 WT 20C 372 383 2 VSHRSGETEDTF 90 1 7.362265368 0.238100148 WT 20C 372 383 2 VSHRSGETEDTF 120 1 7.07628839 0.44664886 WT 20C 372 383 2 VSHRSGETEDTF 180 1 7.122558151 0.401477378 WT 20C 372 383 2 VSHRSGETEDTF 240 1 7.497193964 0.466068376 WT 25C 372 383 2 VSHRSGETEDTF 0 1 0 0.011398411 WT 25C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.601029279 0.062480181 WT 25C 372 383 2 VSHRSGETEDTF 0.333333333 1 5.338037924 0.038416868 WT 25C 372 383 2 VSHRSGETEDTF 0.5 1 5.822527106 0.324221479 WT 25C 372 383 2 VSHRSGETEDTF 0.75 1 5.563117926 0.428495835 WT 25C 372 383 2 VSHRSGETEDTF 1 1 6.117161007 0.588073594 WT 25C 372 383 2 VSHRSGETEDTF 3 1 6.28701581 0.701635544 WT 25C 372 383 2 VSHRSGETEDTF 5 1 6.297762335 0.218680633 WT 25C 372 383 2 VSHRSGETEDTF 10 1 7.079273536 0.254142357 WT 25C 372 383 2 VSHRSGETEDTF 30 1 7.375997039 0.211926019 WT 25C 372 383 2 VSHRSGETEDTF 60 1 7.368832689 WT 25C 372 383 2 VSHRSGETEDTF 90 1 7.604957731 0.159155596 WT 25C 372 383 2 VSHRSGETEDTF 120 1 7.830933277 0.080211043 WT 25C 372 383 2 VSHRSGETEDTF 180 1 7.843172375 0.013087065 WT 25C 372 383 2 VSHRSGETEDTF 240 1 8.088551369 0.263852115 WT 30C 372 383 2 VSHRSGETEDTF 0 1 -3.39E-13 0.016464372 WT 30C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.261618188 0.344907485 WT 30C 372 383 2 VSHRSGETEDTF 0.333333333 1 5.157735111 0.181952418 WT 30C 372 383 2 VSHRSGETEDTF 0.5 1 5.393561638 0.08232186 WT 30C 372 383 2 VSHRSGETEDTF 0.75 1 5.507892726 0.236411495 WT 30C 372 383 2 VSHRSGETEDTF 1 1 5.911782968 0.271451056 WT 30C 372 383 2 VSHRSGETEDTF 3 1 6.039845728 0.062480181 WT 30C 372 383 2 VSHRSGETEDTF 5 1 6.664935283 0.082744023 WT 30C 372 383 2 VSHRSGETEDTF 10 1 6.81687921 0.073878592 WT 30C 372 383 2 VSHRSGETEDTF 30 1 7.133006162 0.153667472 WT 30C 372 383 2 VSHRSGETEDTF 60 1 7.447043512 0.106385173 WT 30C 372 383 2 VSHRSGETEDTF 90 1 7.573912214 0.24949856 WT 30C 372 383 2 VSHRSGETEDTF 120 1 7.761080862 0.016042209 WT 30C 372 383 2 VSHRSGETEDTF 180 1 8.224972537 0.135092283 WT 30C 372 383 2 VSHRSGETEDTF 240 1 8.246167073 0.066279651 WT 40C 372 383 2 VSHRSGETEDTF 0 1 0 0.014775718 WT 40C 372 383 2 VSHRSGETEDTF 0.166666667 1 5.734166787 0.125804688 WT 40C 372 383 2 VSHRSGETEDTF 0.333333333 1 5.964321537 0.232612024 WT 40C 372 383 2 VSHRSGETEDTF 0.5 1 6.18820748 0.349129118 WT 40C 372 383 2 VSHRSGETEDTF 0.75 1 6.406421646 0.233878515 WT 40C 372 383 2 VSHRSGETEDTF 1 1 6.625531357 WT 40C 372 383 2 VSHRSGETEDTF 3 1 7.349130726 0.16464372 WT 40C 372 383 2 VSHRSGETEDTF 5 1 7.561673115 0.040527685 WT 40C 372 383 2 VSHRSGETEDTF 10 1 7.661376989 0.272717546 WT 40C 372 383 2 VSHRSGETEDTF 30 1 8.250047763 0.047282299 WT 40C 372 383 2 VSHRSGETEDTF 60 1 8.355721928 0.256675337 WT 40C 372 383 2 VSHRSGETEDTF 90 1 8.736925062 0.438627756 WT 40C 372 383 2 VSHRSGETEDTF 180 1 9.573362947 0.172664824 WT 40C 372 383 2 VSHRSGETEDTF 240 1 9.609483212 Leu343Ala 10C 383 386 1 FIAD 0 1 0 0.001924231 Leu343Ala 10C 383 386 1 FIAD 0.166666667 1 0.046715196 0.012828207 Leu343Ala 10C 383 386 1 FIAD 0.333333333 1 0.150425955 0.152014248 Leu343Ala 10C 383 386 1 FIAD 0.5 1 0.065083793 0.049495497 Leu343Ala 10C 383 386 1 FIAD 0.75 1 0.041952967 0.005238184 Leu343Ala 10C 383 386 1 FIAD 1 1 0.101140667 0.115881466 Leu343Ala 10C 383 386 1 FIAD 3 1 0.144605453 0.171577263 Leu343Ala 10C 383 386 1 FIAD 5 1 0.044220695 0.045647035 Leu343Ala 10C 383 386 1 FIAD 10 1 0.132510904 0.064247935 Leu343Ala 10C 383 386 1 FIAD 30 1 0.033108828 Leu343Ala 10C 383 386 1 FIAD 60 1 0.146721999 0.151907346 Leu343Ala 10C 383 386 1 FIAD 90 1 0.077329524 0.011010877 Leu343Ala 10C 383 386 1 FIAD 120 1 0.080428752 0.053450861 Leu343Ala 10C 383 386 1 FIAD 180 1 0.196763196 0.105725802 Leu343Ala 10C 383 386 1 FIAD 240 1 0.12729513 0.025656413 Leu343Ala 30C 383 386 1 FIAD 0 1 0 0.012079895 Leu343Ala 30C 383 386 1 FIAD 0.166666667 1 0.074835023 0.016569767 Leu343Ala 30C 383 386 1 FIAD 0.333333333 1 0.065990884 0.01368342 Leu343Ala 30C 383 386 1 FIAD 0.5 1 0.14596609 0.007696924 Leu343Ala 30C 383 386 1 FIAD 0.75 1 0.093732756 0.024266691 Leu343Ala 30C 383 386 1 FIAD 1 1 0.148838545 Leu343Ala 30C 383 386 1 FIAD 3 1 0.080806706 0.025015003 Leu343Ala 30C 383 386 1 FIAD 5 1 0.125783311 0.021914853 Leu343Ala 30C 383 386 1 FIAD 10 1 0.088214618 0.014111027 Leu343Ala 30C 383 386 1 FIAD 30 1 0.088516982 Leu343Ala 30C 383 386 1 FIAD 60 1 0.074910614 0.00555889 Leu343Ala 30C 383 386 1 FIAD 90 1 0.101669804 0.011545386 Leu343Ala 30C 383 386 1 FIAD 120 1 0.118980127 0.08819392 Leu343Ala 30C 383 386 1 FIAD 180 1 0.128806948 0.031963615 Leu343Ala 30C 383 386 1 FIAD 240 1 0.115880899 0.033353337 Leu343Ala 40C 383 386 1 FIAD 0 1 0 0.0124006 Leu343Ala 40C 383 386 1 FIAD 0.166666667 1 0.205380563 0.138865336 Leu343Ala 40C 383 386 1 FIAD 0.333333333 1 0.114671444 0.011224681 Leu343Ala 40C 383 386 1 FIAD 0.5 1 0.046564014 Leu343Ala 40C 383 386 1 FIAD 0.75 1 0.194344286 0.197233676 Leu343Ala 40C 383 386 1 FIAD 1 1 0.157531503 0.152869462 Leu343Ala 40C 383 386 1 FIAD 3 1 0.088743754 0.016676669 Leu343Ala 40C 383 386 1 FIAD 5 1 0.195931696 0.138758434 Leu343Ala 40C 383 386 1 FIAD 10 1 0.256933578 0.220965858 Leu343Ala 40C 383 386 1 FIAD 30 1 0.232668889 0.231335325 Leu343Ala 40C 383 386 1 FIAD 60 1 0.163654368 0.052702549 Leu343Ala 40C 383 386 1 FIAD 90 1 0.1800576 0.024159789 Leu343Ala 40C 383 386 1 FIAD 120 1 0.123213219 0.016462865 Leu343Ala 40C 383 386 1 FIAD 180 1 0.317330733 0 Leu343Ala 40C 383 386 1 FIAD 240 1 0.252927259 0.001069017 WT 10C 383 386 1 FIAD 0 1 -4.30E-14 0.027794448 WT 10C 383 386 1 FIAD 0.166666667 1 0.188599376 0.04158477 WT 10C 383 386 1 FIAD 0.333333333 1 0.174841826 0.070448234 WT 10C 383 386 1 FIAD 0.5 1 0.24491462 0.062644409 WT 10C 383 386 1 FIAD 0.75 1 0.238262618 0.129351083 WT 10C 383 386 1 FIAD 1 1 0.188070239 0.068417102 WT 10C 383 386 1 FIAD 3 1 0.278703767 0.000534509 WT 10C 383 386 1 FIAD 5 1 0.199560061 0.106901721 WT 10C 383 386 1 FIAD 10 1 0.23516339 0.025977118 WT 10C 383 386 1 FIAD 30 1 0.216719202 0.131382216 WT 10C 383 386 1 FIAD 60 1 0.225109796 0.058795947 WT 10C 383 386 1 FIAD 90 1 0.172271734 0.066599772 WT 10C 383 386 1 FIAD 120 1 0.193588377 0.076434731 WT 10C 383 386 1 FIAD 180 1 0.193210422 0.113957235 WT 10C 383 386 1 FIAD 240 1 0.203717562 0.035598273 WT 20C 383 386 1 FIAD 0 1 4.30E-14 0.014645536 WT 20C 383 386 1 FIAD 0.166666667 1 0.157607093 WT 20C 383 386 1 FIAD 0.333333333 1 0.166375642 0.098990994 WT 20C 383 386 1 FIAD 0.5 1 0.137575496 0.039232932 WT 20C 383 386 1 FIAD 0.75 1 0.265399763 WT 20C 383 386 1 FIAD 1 1 0.103635168 0.093432104 WT 20C 383 386 1 FIAD 3 1 0.184668647 0.009834958 WT 20C 383 386 1 FIAD 5 1 0.231459434 0.142713798 WT 20C 383 386 1 FIAD 10 1 0.186936375 0.184726175 WT 20C 383 386 1 FIAD 30 1 0.118980127 0.087766313 WT 20C 383 386 1 FIAD 60 1 0.20114747 0.26768191 WT 20C 383 386 1 FIAD 90 1 0.19464665 WT 20C 383 386 1 FIAD 120 1 0.154129911 0.014324831 WT 20C 383 386 1 FIAD 180 1 0.141506225 0.074724303 WT 20C 383 386 1 FIAD 240 1 0.260410761 0.087873215 WT 25C 383 386 1 FIAD 0 1 4.30E-14 0.00374156 WT 25C 383 386 1 FIAD 0.166666667 1 0.29518259 0.049174792 WT 25C 383 386 1 FIAD 0.333333333 1 0.245443757 0.016035258 WT 25C 383 386 1 FIAD 0.5 1 0.325418963 0.054306074 WT 25C 383 386 1 FIAD 0.75 1 0.262300534 0.103373965 WT 25C 383 386 1 FIAD 1 1 0.274848629 0.020845836 WT 25C 383 386 1 FIAD 3 1 0.324058326 0.092363087 WT 25C 383 386 1 FIAD 5 1 0.239698846 0.007269317 WT 25C 383 386 1 FIAD 10 1 0.280442358 0.052488745 WT 25C 383 386 1 FIAD 30 1 0.350212788 0.077396846 WT 25C 383 386 1 FIAD 60 1 0.11678799 WT 25C 383 386 1 FIAD 90 1 0.319447279 0.092897596 WT 25C 383 386 1 FIAD 120 1 0.316574824 0.000962115 WT 25C 383 386 1 FIAD 180 1 0.167131551 WT 25C 383 386 1 FIAD 240 1 0.243553983 0.031749811 WT 30C 383 386 1 FIAD 0 1 0 0.010476369 WT 30C 383 386 1 FIAD 0.166666667 1 0.167963051 0.050457612 WT 30C 383 386 1 FIAD 0.333333333 1 0.162369322 0.09813578 WT 30C 383 386 1 FIAD 0.5 1 0.21082311 0.058902848 WT 30C 383 386 1 FIAD 0.75 1 0.139692043 0.053450861 WT 30C 383 386 1 FIAD 1 1 0.225563341 0.050457612 WT 30C 383 386 1 FIAD 3 1 0.227679888 0.017745686 WT 30C 383 386 1 FIAD 5 1 0.061077473 WT 30C 383 386 1 FIAD 10 1 0.143169225 0.08445236 WT 30C 383 386 1 FIAD 30 1 0.122154946 WT 30C 383 386 1 FIAD 60 1 0.209008927 0.04906789 WT 30C 383 386 1 FIAD 90 1 0.140221179 0.081779817 WT 30C 383 386 1 FIAD 120 1 0.139692043 0.092790694 WT 30C 383 386 1 FIAD 180 1 0.11678799 0.00930045 WT 30C 383 386 1 FIAD 240 1 0.256631215 0.0683102 WT 40C 383 386 1 FIAD 0 1 0 0.004382971 WT 40C 383 386 1 FIAD 0.166666667 1 0.183837147 0.054199173 WT 40C 383 386 1 FIAD 0.333333333 1 0.118148627 WT 40C 383 386 1 FIAD 0.5 1 0.175295372 0.031429106 WT 40C 383 386 1 FIAD 0.75 1 0.208706564 0.067561888 WT 40C 383 386 1 FIAD 1 1 0.181796192 WT 40C 383 386 1 FIAD 3 1 0.312568504 0.06585146 WT 40C 383 386 1 FIAD 5 1 0.311888186 0.021273443 WT 40C 383 386 1 FIAD 10 1 0.182325328 0.093325203 WT 40C 383 386 1 FIAD 30 1 0.279081721 0.081138407 WT 40C 383 386 1 FIAD 60 1 0.213393201 WT 40C 383 386 1 FIAD 90 1 0.324511872 0.093859711 WT 40C 383 386 1 FIAD 180 1 0.196838787 0.044578018 WT 40C 383 386 1 FIAD 240 1 0.261015489 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 0 1 3.18E-13 0.005938359 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.576259483 0.460816647 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.333333333 1 2.611531535 0.05384112 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.5 1 3.130534586 0.251786415 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.75 1 2.459525787 0.289396021 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 1 1 3.109539317 1.646508964 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 3 1 3.274142226 1.177774505 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 5 1 2.513833549 0.658366051 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 10 1 3.671932591 0.437063211 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 30 1 3.335728349 0.587897526 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 60 1 3.466178954 0.893525062 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 90 1 3.295417432 0.937073027 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 120 1 3.701325967 1.192818348 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 180 1 3.759832784 0.356301531 Leu343Ala 10C 387 407 3 LVVGLRTGQIKTGAPARSERL 240 1 4.39472972 0.528118047 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 0 1 3.18E-13 0.051465777 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.490318848 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.447143243 0.141728831 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.5 1 3.748915244 0.547120795 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.75 1 3.208077113 0.45606596 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 1 1 3.540362238 0.355113859 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 3 1 4.040609515 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 5 1 3.916317523 0.298501505 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 10 1 4.253921449 0.406975527 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 30 1 4.183657282 0.266434367 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 60 1 4.33566303 0.5637482 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 90 1 4.532738622 0.291375474 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 120 1 4.979797885 0.176171313 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 180 1 5.313202758 0.216948043 Leu343Ala 20C 387 407 3 LVVGLRTGQIKTGAPARSERL 240 1 5.115567292 0.068093181 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 0 1 0 0.018606858 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.755913667 0.742690747 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.333333333 1 2.986647008 0.47665227 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.5 1 3.814140546 0.422019369 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.75 1 3.152089729 0.502385159 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 1 1 3.196879636 0.808012694 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 3 1 4.197654128 1.075238843 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 5 1 3.494172646 0.265642586 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 10 1 3.501171069 0.624715351 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 30 1 4.125990276 0.31512891 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 60 1 4.528259632 0.258516555 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 90 1 4.435040637 0.396682371 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 120 1 5.210745845 0.535639968 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 180 1 4.749689736 0.981016882 Leu343Ala 25C 387 407 3 LVVGLRTGQIKTGAPARSERL 240 1 4.772644563 0.520200235 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 0 1 0 0.048694543 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.871592934 0.024941107 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.333333333 1 2.933179057 0.219719277 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.5 1 4.069443018 0.567707106 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.75 1 2.989446378 0.104515116 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 1 1 3.460300279 0.847205863 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 3 1 3.295977306 0.330568643 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 5 1 3.70104603 0.779112681 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 10 1 3.73379865 0.259308336 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 30 1 5.324680171 1.303271822 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 60 1 4.124030718 0.069280853 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 90 1 4.673266957 0.191215155 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 120 1 4.501945561 0.91252781 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 180 1 4.78748122 0.383617982 Leu343Ala 30C 387 407 3 LVVGLRTGQIKTGAPARSERL 240 1 4.668228092 0.224469965 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 0 1 3.18E-13 0.005146578 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.561917381 0.309982332 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.752554424 0.636592069 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.5 1 3.562197318 0.609671509 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 0.75 1 3.823938339 0.173795969 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 1 1 3.726240353 0.461608428 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 3 1 3.785307044 0.038401387 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 5 1 4.245243405 0.372928936 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 10 1 4.863344126 0.032463028 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 30 1 4.987356182 0.465171443 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 60 1 5.006391892 0.08036579 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 90 1 4.76480633 0.606504384 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 120 1 4.664868849 0.359072765 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 180 1 5.377028376 1.403432142 Leu343Ala 40C 387 407 3 LVVGLRTGQIKTGAPARSERL 240 1 5.380387619 0.283061772 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 -4.14E-13 0.011328745 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.615835564 0.317204855 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 2.761483423 0.002059772 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 2.895115335 0.127190908 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 2.470915943 0.239963413 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.114315364 1.593748421 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 3.194057567 1.205996382 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 2.462905311 0.574676329 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.872412475 0.423798045 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 3.37830211 0.75490636 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 3.448213083 0.801251225 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 3.318222368 1.006713461 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 3.802137382 1.23895273 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 3.649935369 0.145213911 Leu343Ala 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 4.358512207 0.542749866 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 4.14E-13 0.090115016 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.636590384 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.560361935 0.149848398 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.841826424 0.550988953 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.323684163 0.576221158 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.621169916 0.264680675 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 4.602472372 0.496919944 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.910645038 0.037590835 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 4.309720174 0.410409529 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 4.202304877 0.453149793 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.311176653 0.470142911 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 4.697143481 0.411439415 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 5.098039216 0.233784098 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 5.400986764 0.227604783 Leu343Ala 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 5.139548856 0.150363341 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 0 0.054583952 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.824712801 0.749756931 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.061882134 0.418648616 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.774464289 0.517002719 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.221366541 0.586520017 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.714748666 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 4.206310194 0.924837533 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.548345986 0.34243706 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.356090811 0.80382594 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 4.161159357 0.376938237 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.402206565 0.208036951 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 4.310812533 0.825453544 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 5.230578768 0.553563668 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 4.736832523 1.03452038 Leu343Ala 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 4.596646458 0.788892595 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 4.14E-13 0.036560949 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.844138584 0.118436878 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.0149107 0.282703678 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 4.065395889 0.640589026 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 2.966846906 0.055098895 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.29491871 1.18642855 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 3.241029002 0.335742801 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.642288856 0.688993663 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.724944017 0.222970296 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 5.368944235 1.183338892 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.195386604 0.059218439 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 4.659639157 0.003089658 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 5.275729605 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 4.875926229 0.323384171 Leu343Ala 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 4.627960748 0.192073719 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 0 0.134915052 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.786844357 0.27085999 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.784659639 0.593214275 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.587306789 0.477867055 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.777741366 0.100413875 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.69035265 0.45263485 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 3.860032407 0.060248325 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 4.134578623 0.194133491 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 4.881752144 0.035531063 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 5.020117611 0.651402828 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.785988676 0.031926463 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 4.775429206 0.571071728 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 4.585722869 0.390326754 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 5.268447212 1.336276947 Leu343Ala 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 4.792906949 0.35994512 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 0 0.061278211 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 2.845230943 0.373333637 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 2.652611648 0.04016555 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.36847088 0.013388517 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.200975841 0.445940592 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 2.87945819 0.43512679 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 3.246126677 0.207007065 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.018551896 0.729674156 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.344803102 0.421738274 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 2.972308701 0.7373983 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 3.299652266 0.675090203 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 2.794618312 0.339862345 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 2.877637592 0.494345229 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 3.726036376 0.865104151 WT 10C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 3.662315437 0.20443235 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 4.14E-13 0.23069444 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.011633623 0.447485421 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.068800408 0.021627604 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.237387806 0.350676147 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 2.991607042 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 2.742913321 0.332138201 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 3.466419065 0.849655862 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.060061536 1.338851662 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.86695068 0.922777761 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 3.318950607 0.506188917 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 3.935405174 1.430511507 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 5.023394687 0.130795509 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 3.562546653 0.562317698 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 3.727856974 0.102988589 WT 20C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 3.999490232 0.40371527 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 -4.14E-13 0.226059954 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 4.351958053 0.344496832 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.476250296 0.13800471 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 4.492508238 0.580340701 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 4.471753418 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 4.412401915 0.842961604 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 4.396380651 1.000534146 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.398328691 0.207007065 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 4.039179274 0.732248871 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 4.67784514 0.807430541 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.363245763 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 4.628324868 0.948524908 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 4.83405247 0.023172433 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 5.837930344 WT 25C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 5.013199337 0.74615233 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 0 0.009268973 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.209350593 0.225545011 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.215176507 0.566437242 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.018916016 0.060248325 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.101935296 0.338317516 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 3.18094926 0.163751857 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 2.95264624 0.454694622 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 3.342254265 0.64831317 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 3.206073516 0.10659319 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 3.738052324 0.757996018 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 3.631001147 0.312055426 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 3.796311468 0.29351748 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 120 1 3.854934732 0.151908169 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 4.083965991 0.189499004 WT 30C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 4.370892275 0.243053071 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0 1 0 0.01390346 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.166666667 1 3.410708759 0.238933527 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.333333333 1 3.677972582 1.061812357 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.5 1 3.277440967 0.265710561 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 0.75 1 3.110310048 0.245627786 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 1 1 4.424417864 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 3 1 4.452455077 0.463963595 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 5 1 4.630145466 0.248717443 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 10 1 4.541664391 0.264680675 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 30 1 4.712436507 0.307420939 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 60 1 4.769603292 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 90 1 5.691190125 0.508248689 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 180 1 5.643490451 0.005149429 WT 40C 387 407 4 LVVGLRTGQIKTGAPARSERL 240 1 6.28871047 0.174050716 Leu343Ala 10C 408 413 1 AKLNQL 0 1 0 0.031272932 Leu343Ala 10C 408 413 1 AKLNQL 0.166666667 1 0.254129672 0.017155997 Leu343Ala 10C 408 413 1 AKLNQL 0.333333333 1 0.276589578 0.074604078 Leu343Ala 10C 408 413 1 AKLNQL 0.5 1 0.378283039 0.128817029 Leu343Ala 10C 408 413 1 AKLNQL 0.75 1 0.264805059 0.001078377 Leu343Ala 10C 408 413 1 AKLNQL 1 1 0.400742945 0.258614401 Leu343Ala 10C 408 413 1 AKLNQL 3 1 0.403377131 0.137836182 Leu343Ala 10C 408 413 1 AKLNQL 5 1 0.298980163 0.087838705 Leu343Ala 10C 408 413 1 AKLNQL 10 1 0.389651633 0.074309976 Leu343Ala 10C 408 413 1 AKLNQL 30 1 0.411418332 0.234497971 Leu343Ala 10C 408 413 1 AKLNQL 60 1 0.381125188 0.074015873 Leu343Ala 10C 408 413 1 AKLNQL 90 1 0.295028883 0.102249742 Leu343Ala 10C 408 413 1 AKLNQL 120 1 0.349653591 0.103230085 Leu343Ala 10C 408 413 1 AKLNQL 180 1 0.388750464 0.05558543 Leu343Ala 10C 408 413 1 AKLNQL 240 1 0.509368475 0.035194302 Leu343Ala 30C 408 413 1 AKLNQL 0 1 0 0.009999495 Leu343Ala 30C 408 413 1 AKLNQL 0.166666667 1 0.300297256 0.063134069 Leu343Ala 30C 408 413 1 AKLNQL 0.333333333 1 0.320885503 0.050879785 Leu343Ala 30C 408 413 1 AKLNQL 0.5 1 Leu343Ala 30C 408 413 1 AKLNQL 0.75 1 0.280194254 0.098230337 Leu343Ala 30C 408 413 1 AKLNQL 1 1 0.344731822 0.041076359 Leu343Ala 30C 408 413 1 AKLNQL 3 1 0.321024144 0.016959928 Leu343Ala 30C 408 413 1 AKLNQL 5 1 0.374054477 0.026665321 Leu343Ala 30C 408 413 1 AKLNQL 10 1 0.338423639 0.069996468 Leu343Ala 30C 408 413 1 AKLNQL 30 1 0.256763859 Leu343Ala 30C 408 413 1 AKLNQL 60 1 0.250732958 0.116366677 Leu343Ala 30C 408 413 1 AKLNQL 90 1 0.384244619 0.141659518 Leu343Ala 30C 408 413 1 AKLNQL 120 1 0.231392484 0.005685988 Leu343Ala 30C 408 413 1 AKLNQL 180 1 0.428956468 0.097642132 Leu343Ala 30C 408 413 1 AKLNQL 240 1 0.338284997 0.039409776 Leu343Ala 40C 408 413 1 AKLNQL 0 1 7.88E-14 0.012744455 Leu343Ala 40C 408 413 1 AKLNQL 0.166666667 1 0.45002996 0.046076106 Leu343Ala 40C 408 413 1 AKLNQL 0.333333333 1 0.433392992 0.005293851 Leu343Ala 40C 408 413 1 AKLNQL 0.5 1 0.404347621 0.177540061 Leu343Ala 40C 408 413 1 AKLNQL 0.75 1 0.414052518 0.069114159 Leu343Ala 40C 408 413 1 AKLNQL 1 1 0.385423071 0.198225291 Leu343Ala 40C 408 413 1 AKLNQL 3 1 0.359219848 0.113131546 Leu343Ala 40C 408 413 1 AKLNQL 5 1 0.38140247 0.002548891 Leu343Ala 40C 408 413 1 AKLNQL 10 1 0.294197035 Leu343Ala 40C 408 413 1 AKLNQL 30 1 0.252743258 Leu343Ala 40C 408 413 1 AKLNQL 60 1 0.387572012 0.155384316 Leu343Ala 40C 408 413 1 AKLNQL 90 1 0.340087335 0.021371471 Leu343Ala 40C 408 413 1 AKLNQL 120 1 0.264111852 0.019410785 Leu343Ala 40C 408 413 1 AKLNQL 180 1 0.36448822 0.1782263 Leu343Ala 40C 408 413 1 AKLNQL 240 1 0.337106545 0.022449848 WT 10C 408 413 1 AKLNQL 0 1 7.88E-14 0.037743193 WT 10C 408 413 1 AKLNQL 0.166666667 1 0.369964555 0.078819552 WT 10C 408 413 1 AKLNQL 0.333333333 1 0.418073119 0.096661789 WT 10C 408 413 1 AKLNQL 0.5 1 0.459665536 0.038429433 WT 10C 408 413 1 AKLNQL 0.75 1 0.501951161 0.143718238 WT 10C 408 413 1 AKLNQL 1 1 0.365250748 0.125679932 WT 10C 408 413 1 AKLNQL 3 1 0.486284684 0.006862399 WT 10C 408 413 1 AKLNQL 5 1 0.510408286 0.209009061 WT 10C 408 413 1 AKLNQL 10 1 0.634908256 0.128817029 WT 10C 408 413 1 AKLNQL 30 1 0.37537157 0.144502512 WT 10C 408 413 1 AKLNQL 60 1 0.468469265 0.10930821 WT 10C 408 413 1 AKLNQL 90 1 0.370588442 0.103818291 WT 10C 408 413 1 AKLNQL 120 1 0.346880764 0.129111132 WT 10C 408 413 1 AKLNQL 180 1 0.566211446 0.076368695 WT 10C 408 413 1 AKLNQL 240 1 0.569192236 0.008430947 WT 20C 408 413 1 AKLNQL 0 1 0 0.022155745 WT 20C 408 413 1 AKLNQL 0.166666667 1 0.46597372 0.040390119 WT 20C 408 413 1 AKLNQL 0.333333333 1 0.477619597 0.068820057 WT 20C 408 413 1 AKLNQL 0.5 1 0.577995965 0.082152717 WT 20C 408 413 1 AKLNQL 0.75 1 0.556437228 0.267535519 WT 20C 408 413 1 AKLNQL 1 1 0.501743199 0.288416818 WT 20C 408 413 1 AKLNQL 3 1 0.529610119 0.140189004 WT 20C 408 413 1 AKLNQL 5 1 0.498693088 0.081368443 WT 20C 408 413 1 AKLNQL 10 1 0.489681398 WT 20C 408 413 1 AKLNQL 30 1 0.52669865 0.283711174 WT 20C 408 413 1 AKLNQL 60 1 0.477758238 0.254104824 WT 20C 408 413 1 AKLNQL 90 1 0.731679948 0.003627268 WT 20C 408 413 1 AKLNQL 120 1 0.506387686 0.064408515 WT 20C 408 413 1 AKLNQL 180 1 0.425005188 0.017155997 WT 20C 408 413 1 AKLNQL 240 1 0.522816691 0.078427415 WT 25C 408 413 1 AKLNQL 0 1 0 0.015979586 WT 25C 408 413 1 AKLNQL 0.166666667 1 0.684749837 0.062839966 WT 25C 408 413 1 AKLNQL 0.333333333 1 0.565379598 0.097544097 WT 25C 408 413 1 AKLNQL 0.5 1 0.655912427 0.077741175 WT 25C 408 413 1 AKLNQL 0.75 1 0.757051323 WT 25C 408 413 1 AKLNQL 1 1 0.672410753 0.078525449 WT 25C 408 413 1 AKLNQL 3 1 0.680382633 0.114700094 WT 25C 408 413 1 AKLNQL 5 1 0.786027374 WT 25C 408 413 1 AKLNQL 10 1 0.571757102 0.076564764 WT 25C 408 413 1 AKLNQL 30 1 0.715597547 0.058428424 WT 25C 408 413 1 AKLNQL 60 1 0.539107054 WT 25C 408 413 1 AKLNQL 90 1 0.622430531 0.210773678 WT 25C 408 413 1 AKLNQL 120 1 0.644127909 0.022645916 WT 25C 408 413 1 AKLNQL 180 1 0.474084241 WT 25C 408 413 1 AKLNQL 240 1 0.665755966 0.061467486 WT 30C 408 413 1 AKLNQL 0 1 -7.88E-14 0.020881299 WT 30C 408 413 1 AKLNQL 0.166666667 1 0.545900482 0.034508063 WT 30C 408 413 1 AKLNQL 0.333333333 1 0.515676659 0.221753516 WT 30C 408 413 1 AKLNQL 0.5 1 0.462091761 0.116758814 WT 30C 408 413 1 AKLNQL 0.75 1 0.532521588 0.072055187 WT 30C 408 413 1 AKLNQL 1 1 0.475609296 0.080192032 WT 30C 408 413 1 AKLNQL 3 1 0.496266864 0.249791317 WT 30C 408 413 1 AKLNQL 5 1 0.469855679 0.163423126 WT 30C 408 413 1 AKLNQL 10 1 0.455714257 0.099504783 WT 30C 408 413 1 AKLNQL 30 1 0.513735679 0.089603322 WT 30C 408 413 1 AKLNQL 60 1 0.437344272 0.143130032 WT 30C 408 413 1 AKLNQL 90 1 0.431729296 0.08911315 WT 30C 408 413 1 AKLNQL 120 1 0.434086199 0.083035026 WT 30C 408 413 1 AKLNQL 180 1 0.494117922 0.074800147 WT 30C 408 413 1 AKLNQL 240 1 0.468399944 0.024704636 WT 40C 408 413 1 AKLNQL 0 1 -7.88E-14 0.012058215 WT 40C 408 413 1 AKLNQL 0.166666667 1 0.568360388 0.128228823 WT 40C 408 413 1 AKLNQL 0.333333333 1 0.727520706 0.083329128 WT 40C 408 413 1 AKLNQL 0.5 1 0.590612331 0.084995711 WT 40C 408 413 1 AKLNQL 0.75 1 0.580699472 0.166854325 WT 40C 408 413 1 AKLNQL 1 1 WT 40C 408 413 1 AKLNQL 3 1 0.605238998 0 WT 40C 408 413 1 AKLNQL 5 1 0.671024339 0.106955387 WT 40C 408 413 1 AKLNQL 10 1 0.631858146 0.138424387 WT 40C 408 413 1 AKLNQL 30 1 0.661666045 0.073133564 WT 40C 408 413 1 AKLNQL 60 1 1.033710221 0.00735257 WT 40C 408 413 1 AKLNQL 90 1 0.738265414 0.033233617 WT 40C 408 413 1 AKLNQL 180 1 0.944286523 0.629085902 WT 40C 408 413 1 AKLNQL 240 1 1.022549589 0.416057436 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 0 1 3.44E-13 0.231036319 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 0.166666667 1 4.210944649 0.174988619 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 0.333333333 1 4.453272881 0.048774334 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 0.5 1 4.727972051 0.639200481 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 0.75 1 4.59122753 0.118085229 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 1 1 4.979678779 1.310061495 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 3 1 5.722395146 0.459077722 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 5 1 5.433174434 0.68412421 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 10 1 6.885026103 0.016258111 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 30 1 7.39721299 0.271681597 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 60 1 7.58357278 0.207504842 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 90 1 7.480409325 0.415009683 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 120 1 8.006210158 0.464639707 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 180 1 7.934207512 0.169426634 Leu343Ala 10C 414 426 2 LRIEEELGDNAVF 240 1 8.720488506 0.340992492 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 0 1 0 0.015402421 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 0.166666667 1 4.935509089 0.12407506 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 0.333333333 1 5.007209202 0.761136316 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 0.5 1 5.716041971 0.360245519 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 0.75 1 5.555094881 0.080434866 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 1 1 5.941125872 0.132631961 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 3 1 6.812720925 0.115946004 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 5 1 6.460270999 0.077867796 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 10 1 7.501586574 0.284089103 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 30 1 8.018613975 0.037222518 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 60 1 8.538364166 0.168143098 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 90 1 8.633661786 0.207076997 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 120 1 8.997607932 0.750868035 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 180 1 9.606000036 0.00427845 Leu343Ala 20C 414 426 2 LRIEEELGDNAVF 240 1 9.339166701 0.49544455 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 0 1 0 0.033799758 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 0.166666667 1 5.365709771 0.124502905 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 0.333333333 1 4.838698809 0.541223968 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 0.5 1 6.014036115 0.079579176 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 0.75 1 5.613483581 0.020536562 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 1 1 5.448906105 0.545074574 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 3 1 6.793358869 0.12493075 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 5 1 6.779139859 0.391478206 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 10 1 7.311293867 0.033371913 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 30 1 8.127828073 0.135626876 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 60 1 8.573760425 0.099687893 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 90 1 8.509623614 0.050057869 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 120 1 9.002448446 0.776110892 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 180 1 9.164605665 0.591281837 Leu343Ala 25C 414 426 2 LRIEEELGDNAVF 240 1 9.034819384 0.102254963 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 0 1 0 0.034655448 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 0.166666667 1 5.92085622 0.151457142 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 0.333333333 1 5.903914421 0.144611622 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 0.5 1 6.895917259 0.302486439 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 0.75 1 5.992858866 0.153168522 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 1 1 6.919212233 0.482609199 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 3 1 7.751175577 1.059344305 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 5 1 8.280001731 1.019982562 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 10 1 8.52323756 0.559621305 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 30 1 8.948597728 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 60 1 9.216641191 0.088136077 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 90 1 9.815957331 0.353827843 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 120 1 10.15902876 0.526677237 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 180 1 10.26098209 0.24900581 Leu343Ala 30C 414 426 2 LRIEEELGDNAVF 240 1 9.614773467 0.442391766 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 0 1 0 0.231464164 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 0.166666667 1 6.197370582 0.138621791 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 0.333333333 1 6.518962231 0.537801208 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 0.5 1 6.484776101 0.411586923 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 0.75 1 6.906808416 0.168998789 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 1 1 6.992425007 0.008556901 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 3 1 6.800922172 0.80392082 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 5 1 7.856759288 0.757713556 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 10 1 8.247933326 0.315749635 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 30 1 8.989742097 0.275532202 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 60 1 9.126184085 0.070594431 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 90 1 9.414194668 0.244299515 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 120 1 9.1809424 0.422710894 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 180 1 9.547308803 0.611390554 Leu343Ala 40C 414 426 2 LRIEEELGDNAVF 240 1 9.906414436 0.337141887 WT 10C 414 426 2 LRIEEELGDNAVF 0 1 -3.44E-13 0.048346489 WT 10C 414 426 2 LRIEEELGDNAVF 0.166666667 1 3.689076733 0.716640433 WT 10C 414 426 2 LRIEEELGDNAVF 0.333333333 1 3.742927452 0.710650602 WT 10C 414 426 2 LRIEEELGDNAVF 0.5 1 4.774259466 0.11979661 WT 10C 414 426 2 LRIEEELGDNAVF 0.75 1 4.964552173 0.278527117 WT 10C 414 426 2 LRIEEELGDNAVF 1 1 4.435726018 0.271681597 WT 10C 414 426 2 LRIEEELGDNAVF 3 1 5.6503925 0.305481355 WT 10C 414 426 2 LRIEEELGDNAVF 5 1 5.620744352 0.168570944 WT 10C 414 426 2 LRIEEELGDNAVF 10 1 6.68959035 0.329012831 WT 10C 414 426 2 LRIEEELGDNAVF 30 1 7.409616808 0.293073849 WT 10C 414 426 2 LRIEEELGDNAVF 60 1 7.638936158 0.366663194 WT 10C 414 426 2 LRIEEELGDNAVF 90 1 7.445315598 0.321311621 WT 10C 414 426 2 LRIEEELGDNAVF 120 1 7.281343187 0.024387167 WT 10C 414 426 2 LRIEEELGDNAVF 180 1 7.988360763 0.511702661 WT 10C 414 426 2 LRIEEELGDNAVF 240 1 7.65618049 0.325162226 WT 20C 414 426 2 LRIEEELGDNAVF 0 1 3.44E-13 0.133915496 WT 20C 414 426 2 LRIEEELGDNAVF 0.166666667 1 4.286880212 0.68241283 WT 20C 414 426 2 LRIEEELGDNAVF 0.333333333 1 4.800277229 0.413298303 WT 20C 414 426 2 LRIEEELGDNAVF 0.5 1 4.963342044 0.963079172 WT 20C 414 426 2 LRIEEELGDNAVF 0.75 1 5.237133617 0.468918158 WT 20C 414 426 2 LRIEEELGDNAVF 1 1 4.815706367 0.091130992 WT 20C 414 426 2 LRIEEELGDNAVF 3 1 5.720277421 1.265137767 WT 20C 414 426 2 LRIEEELGDNAVF 5 1 6.028255124 1.131650116 WT 20C 414 426 2 LRIEEELGDNAVF 10 1 6.774904409 0.204937771 WT 20C 414 426 2 LRIEEELGDNAVF 30 1 7.225979808 0.151457142 WT 20C 414 426 2 LRIEEELGDNAVF 60 1 7.621994359 0.294785229 WT 20C 414 426 2 LRIEEELGDNAVF 90 1 8.495707137 0.510419126 WT 20C 414 426 2 LRIEEELGDNAVF 120 1 7.874911216 0.665299028 WT 20C 414 426 2 LRIEEELGDNAVF 180 1 8.180468662 0.470201693 WT 20C 414 426 2 LRIEEELGDNAVF 240 1 8.496917265 0.377787165 WT 25C 414 426 2 LRIEEELGDNAVF 0 1 3.44E-13 0.179267069 WT 25C 414 426 2 LRIEEELGDNAVF 0.166666667 1 5.274647601 0.109528329 WT 25C 414 426 2 LRIEEELGDNAVF 0.333333333 1 4.696206178 0.332863437 WT 25C 414 426 2 LRIEEELGDNAVF 0.5 1 5.51364798 0.450948666 WT 25C 414 426 2 LRIEEELGDNAVF 0.75 1 4.99268766 0.478330748 WT 25C 414 426 2 LRIEEELGDNAVF 1 1 5.74145467 0.531811378 WT 25C 414 426 2 LRIEEELGDNAVF 3 1 6.36497338 0.823173846 WT 25C 414 426 2 LRIEEELGDNAVF 5 1 5.597449378 0.06631598 WT 25C 414 426 2 LRIEEELGDNAVF 10 1 6.865966579 0.104394188 WT 25C 414 426 2 LRIEEELGDNAVF 30 1 7.949939183 0.600266583 WT 25C 414 426 2 LRIEEELGDNAVF 60 1 7.013602256 WT 25C 414 426 2 LRIEEELGDNAVF 90 1 7.916963181 0.215633897 WT 25C 414 426 2 LRIEEELGDNAVF 120 1 8.004394966 0.146750847 WT 25C 414 426 2 LRIEEELGDNAVF 180 1 7.405078826 0.586147697 WT 25C 414 426 2 LRIEEELGDNAVF 240 1 8.529590735 0.18867966 WT 30C 414 426 2 LRIEEELGDNAVF 0 1 0 0.011979661 WT 30C 414 426 2 LRIEEELGDNAVF 0.166666667 1 5.542691064 0.595132443 WT 30C 414 426 2 LRIEEELGDNAVF 0.333333333 1 5.403526286 0.366663194 WT 30C 414 426 2 LRIEEELGDNAVF 0.5 1 5.188425945 0.234459079 WT 30C 414 426 2 LRIEEELGDNAVF 0.75 1 6.004355087 0.459077722 WT 30C 414 426 2 LRIEEELGDNAVF 1 1 6.557686343 0.6203753 WT 30C 414 426 2 LRIEEELGDNAVF 3 1 6.644815595 0.057331235 WT 30C 414 426 2 LRIEEELGDNAVF 5 1 7.507939748 0.151884987 WT 30C 414 426 2 LRIEEELGDNAVF 10 1 7.452576369 0.071022276 WT 30C 414 426 2 LRIEEELGDNAVF 30 1 7.870978298 0.275960047 WT 30C 414 426 2 LRIEEELGDNAVF 60 1 8.550767983 0.316605325 WT 30C 414 426 2 LRIEEELGDNAVF 90 1 8.790373426 0.195953026 WT 30C 414 426 2 LRIEEELGDNAVF 120 1 8.899284992 0.215633897 WT 30C 414 426 2 LRIEEELGDNAVF 180 1 9.154622105 0.147178692 WT 30C 414 426 2 LRIEEELGDNAVF 240 1 9.040870026 0.340564647 WT 40C 414 426 2 LRIEEELGDNAVF 0 1 -3.44E-13 0.043212348 WT 40C 414 426 2 LRIEEELGDNAVF 0.166666667 1 6.06032353 0.211783292 WT 40C 414 426 2 LRIEEELGDNAVF 0.333333333 1 6.768248702 0.517264647 WT 40C 414 426 2 LRIEEELGDNAVF 0.5 1 6.848117183 0.658453508 WT 40C 414 426 2 LRIEEELGDNAVF 0.75 1 6.202513628 0.075728571 WT 40C 414 426 2 LRIEEELGDNAVF 1 1 7.602934836 WT 40C 414 426 2 LRIEEELGDNAVF 3 1 8.240975087 0.321311621 WT 40C 414 426 2 LRIEEELGDNAVF 5 1 8.389215829 0.040645278 WT 40C 414 426 2 LRIEEELGDNAVF 10 1 8.136601504 0.159158353 WT 40C 414 426 2 LRIEEELGDNAVF 30 1 9.176101886 0.136910411 WT 40C 414 426 2 LRIEEELGDNAVF 60 1 8.774339224 WT 40C 414 426 2 LRIEEELGDNAVF 90 1 9.994148752 0.184829055 WT 40C 414 426 2 LRIEEELGDNAVF 180 1 9.493458085 0.742311135 WT 40C 414 426 2 LRIEEELGDNAVF 240 1 9.546401207 0.776966582 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 -6.27E-13 0.060054453 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.165133032 0.290133204 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 6.554486634 0.695695747 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 6.621768701 0.572467128 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 6.900823833 0.645780357 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 6.733721649 0.278434284 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 6.785561931 0.869619684 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 6.670300028 0.076432941 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 7.476030358 0.606004031 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 7.568681074 0.744831209 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.655816866 1.176911303 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 7.543312426 0.971010319 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 7.599564646 1.038084125 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 8.698688909 Leu343Ala 10C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.353047563 0.770568832 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 Leu343Ala 20C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 Leu343Ala 25C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 0 0.034316831 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.938876805 0.502273611 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 7.003952903 0.57558684 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 7.092743172 1.027165133 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 7.442389325 0.680877116 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 7.815198157 1.148833896 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 7.53504004 0.902376658 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 7.52566467 0.847001773 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 7.54441541 0.446118797 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 7.703796701 1.011566574 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.288522958 0.834522925 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 7.489817667 0.682436971 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 7.837257851 0.365786217 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 7.389446059 0.167684513 Leu343Ala 30C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.385585612 0.294812772 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 Leu343Ala 40C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 6.27E-13 0.036656614 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.194913619 0.758089984 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 6.317344922 0.875079179 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 6.213112867 1.100478362 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 6.32396283 0.992068374 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 6.330580738 1.099698434 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 6.645482872 0.733912217 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 6.610187362 0.699595387 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 6.708904493 0.928114281 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 6.7011836 1.414789333 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.10873645 0.866499972 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 7.216828952 0.782267751 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 7.058550646 0.723773154 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 7.012776781 1.393731278 WT 10C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 6.920126065 0.896137235 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 0 0.065513949 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.923986512 0.971010319 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 6.549523203 0.875079179 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 6.621217209 0.906276298 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 6.775083576 1.184710582 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 6.865528322 1.273622371 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 6.386281466 1.126215985 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 6.894757417 1.625369884 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 6.753575374 1.336796536 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 7.290728927 0.893797451 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.092743172 1.049003116 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 6.880970108 0.838422565 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 7.182084933 1.356294735 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 7.050829753 1.130115625 WT 20C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.056896169 1.717401384 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 0 0.08423222 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.710007478 0.999087726 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 6.725449264 1.298580066 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 6.945494713 1.310278985 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 6.73868508 1.261143523 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 7.07344094 1.057582324 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 6.686844799 1.749378431 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 6.890345478 1.134015265 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 7.29128042 1.300139922 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 7.07123497 1.561415791 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.501399007 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 7.259845355 1.19328979 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 7.365180395 1.540357736 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 7.604528077 1.006887006 WT 25C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.285214004 0.772128688 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 -6.27E-13 0.074093157 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 6.447497117 1.174571519 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 6.493822475 1.43506746 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 6.683535845 1.406990053 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 6.569376928 1.128555769 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 6.710007478 1.433507604 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 6.785561931 1.490442346 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 6.844571612 1.386711926 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 6.706147031 1.502921193 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 6.817548487 1.527878888 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.023255135 1.734559799 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 6.873249215 1.396071062 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 120 1 6.831887288 1.325097617 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 7.037593937 1.611331181 WT 30C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.117560328 1.590273125 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0 1 6.27E-13 0.062394237 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.166666667 1 7.011122304 0.874299251 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.333333333 1 7.212968505 0.693355963 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.5 1 7.064617062 0.881318603 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 0.75 1 7.233373722 0.904716442 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 1 1 6.735927619 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 3 1 7.258190878 0.726112938 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 5 1 7.407093814 0.815024726 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 10 1 7.181533441 1.082540019 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 30 1 7.635411649 0.938253345 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 60 1 7.248264016 0.802545878 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 90 1 7.603425092 0.894577379 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 180 1 8.1438876 1.449886091 WT 40C 427 445 4 AGENFHHGDKLLEHHHHHH 240 1 7.878619777 0.620822662