Conidiomata pycnidial, globose, 5–10 pycnidia grouped together, dark brown to black, exuding creamy to yellowish conidial droplets from central ostioles. Conidiophores hyaline, smooth, densely aggregated, branched, cylindric-clavate, 20.5–32.0 × 1.8–2.4 μm. Conidiogenous cells phialidic, hyaline, terminal, cylindrical, straight, 14.0–20.5 × 1.5–2.0 μm, tapered towards apex. Alpha conidia hyaline, aseptate, fusiform, tapering towards both ends, guttulate, 7.3–9.3 × 1.8–2.3 μm (mean = 8.5 × 2.0 μm, n = 20). Beta conidia hyaline, aseptate, filiform, curved, 22.0–28.5 × 1.0–2.0 μm (mean = 27.2 × 1.3 μm, n = 20). Gamma conidia not observed. Sexual morph not observed.

Cultures incubated on PDA at 25 °C in darkness, growth rate 12.0–13.5 mm diam/day, cottony with abundant aerial mycelium, sparse at margin. With several concentric rings of dense hyphae, white on surface, white to pale brown on reverse.

China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 19 April 2019, S.T. Huang. On diseased leaves of Litchi chinensis (Sapindaceae) HSAUP194.21, living culture SAUCC194.21.

Diaporthe osmanthi was originally described from the leaves of Osmanthus fragrans (Oleaceae) in Guangxi province, China (Long et al. 2019). In the present study, phylogenetic analyses (Fig. 1) indicated that the strain SAUCC194.21 is closely related to Diaporthe osmanthi and D. podocarpi-macrophylli (Gao et al. 2017). Morphological comparison indicated that this strain was most similar to D. osmanthi by the size of alpha conidia and beta conidia. We therefore identify this strain as belonging to D. osmanthi.