LOCUS pBAD>sf.GFP_(sc101-cat) 5497 bp DNA circular UNA 29-FEB-2020 DEFINITION Gibson Assembly of pGERC-GFP-Lib - sfGFP into pMS_260 pBAD vector. ACCESSION urn.local...fj-bmf5j0b VERSION urn.local...fj-bmf5j0b KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers terminator complement(1..85) /created_by="User" /label="araC native terminator" primer_bind 1..20 /Sequence="AATGTGCCTGTCAAATGGAC" /Transferred_From="oDK_052" /Transferred_Similarity="100.00%" /label="oDK_052" gene complement(86..964) /Imported_from="Pla sMapper" /X-Transferred_From="AraC" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraC" cds complement(86..1015) /codon_start=1 /transl_table=11 /product="araC" /protein_id="AIU94532.1" /Transferred_Translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSF NAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILL FPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFS DLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDH LADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIA TVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" /label="araC CDS" misc_feature 978..1290 /created_by="User" /modified_by="User" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="segment of pBAD_33" regulatory 991..1276 /regulatory_class="promoter" /note="PBAD promoter" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" misc_feature 993..1010 /note="misc_feature; operator O2" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" misc_feature 994..1009 /Imported_from="Pla sMapper" /X-Transferred_From="araO2 reg; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="araO2 reg" /note="Geneious type: regulatory sequence" promoter complement(1115..1143) /note="Pc promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; Pc promoter" misc_feature complement(1115..1143) /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="Pc" misc_feature 1151..1172 /note="misc_feature; operator O1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" primer_bind 1198..1217 /Sequence="ATGCCATAGCATTTTTATCC" /Transferred_From="pBAD Forward" /Transferred_Similarity="100.00%" /label="pBAD Forward" gene 1203..1241 /Imported_from="Pla sMapper" /X-Transferred_From="AraI1I2; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraI1I2" misc_feature 1203..1241 /note="misc_feature; operator I2 + I1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" promoter 1238..1266 /Imported_from="Pla sMapper" /X-Transferred_From="ARA prom" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ARA prom" promoter 1240..1267 /note="PBAD promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; PBAD promoter" misc_feature 1240..1267 /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ParaB" misc_feature 1308..1314 /created_by="User" /modified_by="Max_Schubert" /label="suspected RBS" /note="Geneious type: regulatory sequence" CDS 1321..2037 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /label="sfGFP" primer_bind complement(1905..1929) /ApEinfo_revcolor="#b4abac" /ApEinfo_fwdcolor="#b4abac" /label="Lib_R" primer_bind 1924..1948 /ApEinfo_revcolor="#9eafd2" /ApEinfo_fwdcolor="#9eafd2" /label="Vec_lib_F" primer_bind complement(2100..2117) /Sequence="GATTTAATCTGTATCAGG" /Transferred_From="pBAD Reverse" /Transferred_Similarity="100.00%" /label="pBAD Reverse" terminator 2150..2307 /Imported_from="Pla sMapper" /X-Transferred_From="rrnB term" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_76 (pRSFRedGFP henry's v.nat plasmid)" /Transferred_Similarity="97.47%" /label="rrnB term" unsure 2234..2239 /created_by="User" /label="bsaI site" primer_bind 2250..2293 /Sequence="GCGAAATTTTGCCGACTGATCAAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0434" /X-Transferred_Similarity="65.91%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0434" primer_bind 2254..2293 /Sequence="GCGAAATTTTGCCGACTGAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0433" /X-Transferred_Similarity="70.00%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0433" primer_bind 2273..2294 /Sequence="ATAAAACGAAAGGCTCAGTCGA" /Transferred_From="oMS_0388" /Transferred_Similarity="100.00%" /label="oMS_0388" primer_bind 2853..2879 /Sequence="AAGAACCTCAGATCCTTCCGTATTTAG" /Transferred_From="oMS_0460" /Transferred_Similarity="100.00%" /label="oMS_0460" CDS complement(2860..3810) /created_by="User" /label="repA101 CDS" primer_bind complement(3029..3049) /Sequence="CTACCTACGTAACGGACTAAG" /Transferred_From="repA_rev" /Transferred_Similarity="100.00%" /label="repA_rev" regulatory 4566..4671 /regulatory_class="terminator" /note="T0 terminator; terminator T0" /Transferred_From="KM521210, p ECG_MC3 KM521211 (mf-lon mcherry_pdt3)" /Transferred_Similarity="100.00%" /label="T0 terminator regulatory; terminator T0 regulatory" primer_bind 4643..4666 /Sequence="TTACTGGATCTATCAACAGGAGTC" /Transferred_From="oMS_0350" /Transferred_Similarity="100.00%" /label="oMS_0350" primer_bind complement(4687..4719) /Sequence="GGGGCGTAATTGTCTAACAATTCGTTCAAGCCG" /Transferred_From="oDK_049" /Transferred_Similarity="100.00%" /label="oDK_049" primer_bind complement(4689..4709) /Sequence="TGTCTAACAATTCGTTCAAGC" /Transferred_From="oMS_0337" /Transferred_Similarity="100.00%" /label="oMS_0337" primer_bind 4702..4726 /Sequence="GTTAGACAATTACGCCCCGCCCTGC" /Transferred_From="oDK_050" /Transferred_Similarity="100.00%" /label="oDK_050" misc_feature complement(4711..5497) /label="CmR" /note="Geneious type: Antibiotic Resistance" marker complement(4711..5370) /label="CAT marker" cds complement(4711..5370) /codon_start=1 /transl_table=11 /product="CmR" /protein_id="ADC42093.1" /db_xref="GI:288187151" /label="CmR CDS" misc_difference 4786 /created_by="User" /modified_by="User" /label="actually A in sequenced descendants, was G" primer_bind 4868..4889 /Sequence="GCGAAGAAGTTGTCCATATTGG" /Transferred_From="oMS_0383" /Transferred_Similarity="100.00%" /label="oMS_0383" primer_bind complement(5328..5354) /Sequence="CTGGATATACCACCGTTGATATATCCC" /Transferred_From="oMS_0382" /Transferred_Similarity="100.00%" /label="oMS_0382" primer_bind complement(5415..5436) /Sequence="GAAATAAGATCACTACCGGGCG" /Transferred_From="oMS_0222" /Transferred_Similarity="100.00%" /label="oMS_0222" primer_bind 5415..5436 /Sequence="CGCCCGGTAGTGATCTTATTTC" /Transferred_From="oMS_521" /Transferred_Similarity="100.00%" /label="oMS_521" ORIGIN 1 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc 61 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt 121 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 181 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 241 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 301 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 361 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 421 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 481 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 541 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 601 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 661 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 721 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 781 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 841 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 901 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 961 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 1021 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 1081 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 1141 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 1201 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 1261 ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcaag aggataccat 1321 atgcgtaaag gcgaagagct gttcactggt ttcgtcacta ttctggtgga actggatggt 1381 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt 1441 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg 1501 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca catgaagcag 1561 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt 1621 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta 1681 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag 1741 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc 1801 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat 1861 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat 1921 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca catggttctg 1981 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaataatct 2041 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc 2101 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 2161 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 2221 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 2281 aaaggctcag tcgaaagact gaacgctctc ctgagtagga caaatccgcc gccctagacc 2341 tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag 2401 aatcgcagat ccggcttcag gtttgccggc tgaaagcgct atttcttcca gaattgccat 2461 gattttttcc ccacgggagg cgtcactggc tcccgtgttg tcggcagctt tgattcgata 2521 agcagcatcg cctgtttcag gctgtctatg tgtgactgtt gagctgtaac aagttgtctc 2581 aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt tctattaggt 2641 gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc tttaaatgca 2701 ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat 2761 atggacagtt ttccctttga tatctaacgg tgaacagttg ttctactttt gtttgttagt 2821 cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc 2881 cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag 2941 atcatgctta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt 3001 tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta cgtaggtagg aatctgatgt 3061 aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac 3121 gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg 3181 cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa 3241 aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt 3301 aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt 3361 taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag 3421 attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac 3481 taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc 3541 tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc 3601 taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt 3661 gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac 3721 acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc 3781 atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc 3841 actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt 3901 gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc 3961 tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta 4021 tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc 4081 actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct 4141 acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc 4201 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca 4261 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc 4321 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct 4381 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt 4441 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga 4501 ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc 4561 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa 4621 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat 4681 atccgtcggc ttgaacgaat tgttagacaa ttacgccccg ccctgccact catcgcagta 4741 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac 4801 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa 4861 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac 4921 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag 4981 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc 5041 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca 5101 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg 5161 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt 5221 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca 5281 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac 5341 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga 5401 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct 5461 tacgtgccga tcaacgtctc attttcgcca gatatcg // LOCUS pBAD>retron,_RT,_CspRecT_(sc101_cat) 6802 bp DNA circular UNA 29-FEB-2020 DEFINITION Gibson Assembly of PF020 into b15_L ev. ACCESSION urn.local...56-bmf2nj0 VERSION urn.local...56-bmf2nj0 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers terminator complement(1..85) /created_by="User" /label="araC native terminator" gene complement(86..964) /Imported_from="Pla sMapper" /X-Transferred_From="AraC" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraC" cds complement(86..1015) /codon_start=1 /transl_table=11 /product="araC" /protein_id="AIU94532.1" /Transferred_Translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSF NAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILL FPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFS DLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDH LADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIA TVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" /label="araC CDS" misc_feature 978..>1281 /created_by="User" /modified_by="User" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="segment of pBAD_33" regulatory 991..1276 /regulatory_class="promoter" /note="PBAD promoter" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" misc_feature 993..1010 /note="misc_feature; operator O2" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" misc_feature 994..1009 /Imported_from="Pla sMapper" /X-Transferred_From="araO2 reg; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="araO2 reg" /note="Geneious type: regulatory sequence" primer_bind 1088..1115 /Sequence="CATTCTGTAACAAAGCGGGACCAAAGCC" /Transferred_From="oMS_818" /Transferred_Similarity="100.00%" /label="oMS_818" promoter complement(1115..1143) /note="Pc promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; Pc promoter" misc_feature complement(1115..1143) /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="Pc" primer_bind 1119..1140 /Sequence="ACAAAAACGCGTAACAAAAGTG" /Transferred_From="oMS_802" /Transferred_Similarity="100.00%" /label="oMS_802" primer_bind 1148..1166 /Sequence="TCACGGCAGAAAAGTCCAC" /Transferred_From="oMS_806" /Transferred_Similarity="100.00%" /label="oMS_806" misc_feature 1151..1172 /note="misc_feature; operator O1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" gene 1203..1241 /Imported_from="Pla sMapper" /X-Transferred_From="AraI1I2; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraI1I2" misc_feature 1203..1241 /note="misc_feature; operator I2 + I1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" primer_bind 1213..1272 /Sequence="TATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTC TCTACTGTTTCTC" /Transferred_From="oMS_808" /Transferred_Similarity="100.00%" /label="oMS_808" promoter 1238..1266 /Imported_from="Pla sMapper" /X-Transferred_From="ARA prom" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ARA prom" promoter 1240..1267 /note="PBAD promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; PBAD promoter" misc_feature 1240..1267 /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ParaB" primer_bind complement(1257..1281) /Sequence="ACGGGTATGGAGAAACAGTAGAGAG" /Transferred_From="oMS_801" /Transferred_Similarity="100.00%" /label="oMS_801" msr 1290..1520 /modified_by="Max_Schubert" /label="msr86" gene <1290..1303 /locus_tag="ECD_00830" /db_xref="GeneID:8179524" CDS <1290..1303 /locus_tag="ECD_00830" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_003053496.1" /db_xref="GI:254287748" /db_xref="GeneID:8179524" /translation="MNKKFTDEQQQQLIGHLTKKGFYRGANIKITIFLCGGDVANHQS WRHQLSQFLAKFSDVDIFYPEDLFDDLLAGQGQHSLLSLENILAEAVDVIILFPESPG SFTELGAFSNNENLRRKLICIQDAKFKSKRSFINYGPVRLLRKFNSKSVLRCSSNELK EMCDSSIDVARKLRLYKKLMASIKKVRKENKVSKDIGNILYAERFLLPCIYLLDSVNY RTLCELAFKAIKQDDVLSKIIVRSVVSRLINERKILQMTDGYQVTALGASYVRSVFDR KTLDRLRLEIMNFENRRKSTFNYDKIPYAHP" inv_rep 1291..1302 /label="a2" Branched_G 1303 primer_bind 1328..1348 /Sequence="CCTCTGGATGTTGTTTCGGCA" /Transferred_From="oMS_0409" /Transferred_Similarity="100.00%" /label="oMS_0409" primer_bind complement(1329..1348) /Sequence="TGCCGAAACAACATCCAGAG" /Transferred_From="oMS_539" /Transferred_Similarity="100.00%" /label="oMS_539" primer_bind 1350..1371 /Sequence="CCTGCATTGAATCTGAGTTACT" /Transferred_From="oMS_545" /Transferred_Similarity="100.00%" /label="oMS_545" msd complement(1361..1506) /created_by="Max_Schubert" misc_rna <1361..>1365 /label="msr86" primer_bind 1361..1389 /Sequence="TCTGAGTTACTGTCTGTTTTCCTGAATTC" /Transferred_From="oMS_0416" /Transferred_Similarity="100.00%" /label="oMS_0416" primer_bind complement(1362..1383) /Sequence="AGGAAAACAGACAGTAACTCAG" /Transferred_From="oMS_532" /Transferred_Similarity="100.00%" /label="oMS_532" primer_bind complement(1366..1389) /Sequence="GAATTCAGGAAAACAGACAGTAAC" /Transferred_From="oMS_486" /Transferred_Similarity="100.00%" /label="oMS_486" misc_feature 1390..1479 /Original_Bases="TGTCGTTGGTGACGTAATCGGTAAATACCATCCCCATGGTG ACTTGGCCGTCTATGACACGATCGTCCGCATGGCGCAGCCATTCTCGCT" /label="TGTCGTTGGTGACGTAATCGGTAAATACCATCCCCATGGTGACTTGGCCG TCTATGACACGATCGTCCGCATGGCGCAGCCATTCTCGCT" /note="Geneious type: Editing History Replacement" misc_feature complement(1390..1479) /created_by="Max_Schubert" /label="insert donor here (reverse orientation)" primer_bind complement(1480..1506) /Sequence="GTCAGAAAAAACGGGTTTCCTGAATTC" /Transferred_From="oMS_0417" /Transferred_Similarity="100.00%" /label="oMS_0417" primer_bind 1480..1502 /Sequence="GAATTCAGGAAACCCGTTTTTTC" /Transferred_From="oMS_487" /Transferred_Similarity="100.00%" /label="oMS_487" primer_bind 1485..1513 /Sequence="CAGGAAACCCGTTTTTTCTGACGTAAGGG" /Transferred_From="oMS_623" /Transferred_Similarity="100.00%" /label="oMS_623" primer_bind complement(1502..1519) /Sequence="TGCGCACCCTTACGTCAG" /Transferred_From="oMS_757" /Transferred_Similarity="100.00%" /label="oMS_757" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_668" /Transferred_Similarity="100.00%" /label="oMS_668" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_696" /Transferred_Similarity="100.00%" /label="oMS_696" primer_bind 1507..1528 /Sequence="GTAAGGGTGCGCAACTTTCATG" /Transferred_From="oMS_570" /Transferred_Similarity="100.00%" /label="oMS_570" inv_rep complement(1508..1519) /label="a1" cds 1526..2488 /locus_tag="ECD_00831" /codon_start=1 /transl_table=11 /product="retron EC86 RNA-directed DNA polymerase-like protein" /protein_id="ACT42726.1" /db_xref="GI:253977056" /label="retron EC86 RNA-directed DNA polymerase-like protein CDS" primer_bind 1526..1551 /Sequence="ATGAAATCCGCTGAATATTTGAACAC" /Transferred_From="oMS_533" /Transferred_Similarity="100.00%" /label="oMS_533" primer_bind complement(1528..1551) /Sequence="GTGTTCAAATATTCAGCGGATTTC" /Transferred_From="seqR" /Transferred_Similarity="100.00%" /label="seqR" primer_bind 1588..1610 /Sequence="CAATTTGCATGACATGTCTAAGG" /Transferred_From="oMS_540" /Transferred_Similarity="100.00%" /label="oMS_540" primer_bind complement(1588..1610) /Sequence="CCTTAGACATGTCATGCAAATTG" /Transferred_From="oMS_525" /Transferred_Similarity="100.00%" /label="oMS_525" misc_feature 2119 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_difference 2119 /created_by="User" /label="synonymous mutation?" primer_bind complement(2464..2487) /Sequence="TAGGTCTTCGCTTTATTTAAAGGG" /Transferred_From="oMS_522" /Transferred_Similarity="100.00%" /label="oMS_522" primer_bind complement(2465..2486) /Sequence="AGGTCTTCGCTTTATTTAAAGG" /Transferred_From="oMS_559" /Transferred_Similarity="100.00%" /label="oMS_559" gene 2527..3339 /created_by="Tim" /modified_by="Max_Schubert" /label="CspRecT" terminator 3452..3609 /Imported_from="Pla sMapper" /X-Transferred_From="rrnB term" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_76 (pRSFRedGFP henry's v.nat plasmid)" /Transferred_Similarity="97.47%" /label="rrnB term" primer_bind 3552..3595 /Sequence="GCGAAATTTTGCCGACTGATCAAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0434" /X-Transferred_Similarity="65.91%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0434" primer_bind 3556..3595 /Sequence="GCGAAATTTTGCCGACTGAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0433" /X-Transferred_Similarity="70.00%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0433" primer_bind 3575..3596 /Sequence="ATAAAACGAAAGGCTCAGTCGA" /Transferred_From="oMS_0388" /Transferred_Similarity="100.00%" /label="oMS_0388" primer_bind 4158..4184 /Sequence="AAGAACCTCAGATCCTTCCGTATTTAG" /Transferred_From="oMS_0460" /Transferred_Similarity="100.00%" /label="oMS_0460" CDS complement(4165..5115) /created_by="User" /label="repA101 CDS" primer_bind complement(4308..4340) /Sequence="GACTAAGAAAAACACTACACGATGCTTTAACTG" /Transferred_From="oMS_813" /Transferred_Similarity="100.00%" /label="oMS_813" primer_bind complement(4334..4354) /Sequence="CTACCTACGTAACGGACTAAG" /Transferred_From="repA_rev" /Transferred_Similarity="100.00%" /label="repA_rev" primer_bind 4341..4370 /Sequence="CGTTACGTAGGTAGGAATCTGATGTAATGG" /Transferred_From="oMS_812" /Transferred_Similarity="100.00%" /label="oMS_812" primer_bind 4400..4421 /Sequence="ATCTGGTTGTTCTCAAGTTCGG" /Transferred_From="oMS_918" /Transferred_Similarity="100.00%" /label="oMS_918" primer_bind 4840..4864 /Sequence="AAAGCCTTTAACCAAAGGATTCCTG" /Transferred_From="oMS_920" /Transferred_Similarity="100.00%" /label="oMS_920" primer_bind complement(4840..4864) /Sequence="CAGGAATCCTTTGGTTAAAGGCTTT" /Transferred_From="oMS_916" /Transferred_Similarity="100.00%" /label="oMS_916" unsure complement(5117..5373) /created_by="User" /label="important ori stuff" primer_bind complement(5207..5229) /Sequence="GTCTAGCAGAATTTACAGATACC" /Transferred_From="oMS_919" /Transferred_Similarity="100.00%" /label="oMS_919" primer_bind complement(5263..5283) /Sequence="GGTCTAGCGGAATTTACAGAG" /Transferred_From="oMS_917" /Transferred_Similarity="100.00%" /label="oMS_917" unsure complement(5374..5382) /created_by="User" /label="DNAa binding sequence" regulatory 5871..5976 /regulatory_class="terminator" /note="T0 terminator; terminator T0" /Transferred_From="KM521210, p ECG_MC3 KM521211 (mf-lon mcherry_pdt3)" /Transferred_Similarity="100.00%" /label="T0 terminator regulatory; terminator T0 regulatory" primer_bind 5948..5971 /Sequence="TTACTGGATCTATCAACAGGAGTC" /Transferred_From="oMS_0350" /Transferred_Similarity="100.00%" /label="oMS_0350" primer_bind complement(5994..6014) /Sequence="TGTCTAACAATTCGTTCAAGC" /Transferred_From="oMS_0337" /Transferred_Similarity="100.00%" /label="oMS_0337" misc_feature complement(6016..6802) /label="CmR" /note="Geneious type: Antibiotic Resistance" marker complement(6016..6675) /label="CAT marker" cds complement(6016..6675) /codon_start=1 /transl_table=11 /product="CmR" /protein_id="ADC42093.1" /db_xref="GI:288187151" /label="CmR CDS" misc_feature 6091 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" primer_bind 6173..6194 /Sequence="GCGAAGAAGTTGTCCATATTGG" /Transferred_From="oMS_0383" /Transferred_Similarity="100.00%" /label="oMS_0383" primer_bind complement(6588..6677) /Sequence="AAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAA TGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTC" /Transferred_From="oMS_0401" /Transferred_Similarity="100.00%" /label="oMS_0401" primer_bind complement(6633..6659) /Sequence="CTGGATATACCACCGTTGATATATCCC" /Transferred_From="oMS_0382" /Transferred_Similarity="100.00%" /label="oMS_0382" primer_bind complement(6720..6741) /Sequence="GAAATAAGATCACTACCGGGCG" /Transferred_From="oMS_0222" /Transferred_Similarity="100.00%" /label="oMS_0222" primer_bind 6720..6741 /Sequence="CGCCCGGTAGTGATCTTATTTC" /Transferred_From="oMS_521" /Transferred_Similarity="100.00%" /label="oMS_521" ORIGIN 1 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc 61 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt 121 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 181 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 241 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 301 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 361 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 421 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 481 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 541 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 601 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 661 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 721 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 781 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 841 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 901 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 961 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 1021 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 1081 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 1141 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 1201 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 1261 ctactgtttc tccatacccg tttaattaaa tgcgcaccct tagcgagagg tttatcatta 1321 aggtcaacct ctggatgttg tttcggcatc ctgcattgaa tctgagttac tgtctgtttt 1381 cctgaattcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aattcaggaa acccgttttt 1501 tctgacgtaa gggtgcgcaa ctttcatgaa atccgctgaa tatttgaaca cttttagatt 1561 gagaaatctc ggcctacctg tcatgaacaa tttgcatgac atgtctaagg cgactcgcat 1621 atctgttgaa acacttcggt tgttaatcta tacagctgat tttcgctata ggatctacac 1681 tgtagaaaag aaaggcccag agaagagaat gagaaccatt taccaacctt ctcgagaact 1741 taaagcctta caaggatggg ttctacgtaa cattttagat aaactgtcgt catctccttt 1801 ttctattgga tttgaaaagc accaatctat tttgaataat gctaccccgc atattggggc 1861 aaactttata ctgaatattg atttggagga ttttttccca agtttaactg ctaacaaagt 1921 ttttggagtg ttccattctc ttggttataa tcgactaata tcttcagttt tgacaaaaat 1981 atgttgttat aaaaatctgc taccacaagg tgctccatca tcacctaaat tagctaatct 2041 aatatgttct aaacttgatt atcgtattca gggttatgca ggtagtcggg gcttgatata 2101 tacgagatat gccgatgacc tcaccttatc tgcacagtct atgaaaaagg ttgttaaagc 2161 acgtgatttt ttattttcta taatcccaag tgaaggattg gttattaact caaaaaaaac 2221 ttgtattagt gggcctcgta gtcagaggaa agttacaggt ttagttattt cacaagagaa 2281 agttgggata ggtagagaaa aatataaaga aattagagca aagatacatc atatattttg 2341 cggtaagtct tctgagatag aacacgttag gggatggttg tcatttattt taagtgtgga 2401 ttcaaaaagc cataggagat taataactta tattagcaaa ttagaaaaaa aatatggaaa 2461 gaacccttta aataaagcga agacctagga tcgaaataat tttgtttaac tttaaggagg 2521 tttggaatga accaaatcgt gaagttcact gacgactctg gcctggcggt tcaagttact 2581 ccagacgatg ttcgccgtta tatctgtgag aacgctactg aaaaagaggt gggcctcttt 2641 ctgcaactct gtcagactca acgtctgaat ccgtttgtga aagacgctta cctggtgaaa 2701 tacggcggtg ctccagcttc tatgattact tcctatcaag tttttaaccg tcgcgcgtgt 2761 cgtgatgcta actatgatgg tatcaaatct ggtgtggttg ttctgcgtga cggtgatgtt 2821 gtgcataaac gtggtgctgc gtgctacaaa aaggcgggtg aggagctcat cggtggttgg 2881 gcggaagttc gctttaagga tggccgcgag actgcgtatg ctgaggtggc gctcgacgac 2941 tattccaccg gcaaatctaa ttgggcgaaa atgccgggtg ttatgatcga aaaatgcgcg 3001 aaggctgctg cttggcgcct cgcgttcccg gacacttttc agggcatgta cgctgcggag 3061 gaaatggatc aagcgcaaca gccagaacag gtgcgcgctc aggcggagca accagtggat 3121 ctccagccaa tccgcgaact cttcaagcca tattgcgaac acttcggcat cactccggct 3181 gagggtatga ctgctgtttg tggtgcggtg ggcgctgaag gcatgcactc tatgaccgag 3241 cagcaagctc gccgtgctcg cgcttggatg gaggaagaaa tggctgcgcc agctgtggaa 3301 gcggagtatg aggttgttga cgagggcgag gtgttttaat ctagagtcga cctgcaggca 3361 tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca 3421 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca 3481 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct 3541 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3601 ctgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggtac gggttttgct 3661 gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc 3721 agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg 3781 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt 3841 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca 3901 tgttctagtt gctttgtttt actggtttca cccgttctat taggtgttac atgctgttca 3961 tctgttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa aactcgtaaa 4021 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc 4081 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg 4141 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag 4201 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg 4261 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc 4321 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat 4381 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct 4441 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa 4501 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag 4561 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct 4621 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata 4681 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa 4741 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc 4801 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt 4861 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc 4921 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg 4981 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag 5041 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac 5101 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag 5161 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt 5221 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag 5281 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga 5341 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt 5401 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta 5461 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt 5521 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc 5581 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg 5641 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg 5701 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt 5761 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc 5821 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc 5881 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct 5941 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatccg tcggcttgaa 6001 cgaattgtta gacaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 6061 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg 6121 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 6181 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 6241 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca 6301 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca 6361 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc 6421 ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag 6481 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt 6541 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg 6601 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt 6661 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac 6721 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac 6781 gtctcatttt cgccagatat cg // LOCUS pBAD>retron,_RT,_beta_(sc101_cat) 6775 bp DNA circular UNA 29-FEB-2020 DEFINITION Gibson Assembly of 1547.rif SCRIBE into pMS_260 pBAD vector. ACCESSION urn.local...g-bmf1q10 VERSION urn.local...g-bmf1q10 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers terminator complement(1..85) /created_by="User" /label="araC native terminator" gene complement(86..964) /Imported_from="Pla sMapper" /X-Transferred_From="AraC" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraC" cds complement(86..1015) /codon_start=1 /transl_table=11 /product="araC" /protein_id="AIU94532.1" /Transferred_Translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSF NAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILL FPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFS DLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDH LADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIA TVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" /label="araC CDS" misc_feature 978..>1280 /created_by="User" /modified_by="User" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="segment of pBAD_33" regulatory 991..1276 /regulatory_class="promoter" /note="PBAD promoter" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" misc_feature 993..1010 /note="misc_feature; operator O2" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" misc_feature 994..1009 /Imported_from="Pla sMapper" /X-Transferred_From="araO2 reg; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="araO2 reg" /note="Geneious type: regulatory sequence" promoter complement(1115..1143) /note="Pc promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; Pc promoter" misc_feature complement(1115..1143) /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="Pc" misc_feature 1151..1172 /note="misc_feature; operator O1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" primer_bind 1198..1217 /Sequence="ATGCCATAGCATTTTTATCC" /Transferred_From="pBAD Forward" /Transferred_Similarity="100.00%" /label="pBAD Forward" gene 1203..1241 /Imported_from="Pla sMapper" /X-Transferred_From="AraI1I2; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraI1I2" misc_feature 1203..1241 /note="misc_feature; operator I2 + I1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" promoter 1238..1266 /Imported_from="Pla sMapper" /X-Transferred_From="ARA prom" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ARA prom" promoter 1240..1267 /note="PBAD promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; PBAD promoter" misc_feature 1240..1267 /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ParaB" misc_feature 1289..1290 /label="AAGAATTCATTAAAGAGGAGAAAGGTACCTAGCTATGTTAGGAGCGTCTT TGATAGAAAGACACTTGACCGATTGCGGCTTGAGATTATGAATTTTGAAAACCGTAGA AAATCAACATTTAACTATGATAAGATTCCGT" /note="Geneious type: Editing History Deletion" msr 1290..1520 /product="msr" /function="primer for the Ec86 RT" /modified_by="Max_Schubert" inv_rep 1291..1302 /label="a2" Branched_G 1303 primer_bind 1328..1348 /Sequence="CCTCTGGATGTTGTTTCGGCA" /Transferred_From="oMS_0409" /Transferred_Similarity="100.00%" /label="oMS_0409" primer_bind complement(1329..1348) /Sequence="TGCCGAAACAACATCCAGAG" /Transferred_From="oMS_539" /Transferred_Similarity="100.00%" /label="oMS_539" primer_bind 1350..1371 /Sequence="CCTGCATTGAATCTGAGTTACT" /Transferred_From="oMS_545" /Transferred_Similarity="100.00%" /label="oMS_545" msd complement(1361..1506) /created_by="Max_Schubert" primer_bind 1361..1389 /Sequence="TCTGAGTTACTGTCTGTTTTCCTGAATTC" /Transferred_From="oMS_0416" /Transferred_Similarity="100.00%" /label="oMS_0416" primer_bind complement(1362..1383) /Sequence="AGGAAAACAGACAGTAACTCAG" /Transferred_From="oMS_532" /Transferred_Similarity="100.00%" /label="oMS_532" primer_bind complement(1366..1389) /Sequence="GAATTCAGGAAAACAGACAGTAAC" /Transferred_From="oMS_486" /Transferred_Similarity="100.00%" /label="oMS_486" misc_feature complement(1390..1479) /created_by="Max_Schubert" /label="insert donor here (reverse orientation)" primer_bind complement(1480..1506) /Sequence="GTCAGAAAAAACGGGTTTCCTGAATTC" /Transferred_From="oMS_0417" /Transferred_Similarity="100.00%" /label="oMS_0417" primer_bind 1480..1502 /Sequence="GAATTCAGGAAACCCGTTTTTTC" /Transferred_From="oMS_487" /Transferred_Similarity="100.00%" /label="oMS_487" primer_bind 1485..1513 /Sequence="CAGGAAACCCGTTTTTTCTGACGTAAGGG" /Transferred_From="oMS_623" /Transferred_Similarity="100.00%" /label="oMS_623" primer_bind complement(1502..1519) /Sequence="TGCGCACCCTTACGTCAG" /Transferred_From="oMS_757" /Transferred_Similarity="100.00%" /label="oMS_757" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_668" /Transferred_Similarity="100.00%" /label="oMS_668" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_696" /Transferred_Similarity="100.00%" /label="oMS_696" primer_bind complement(1506..1543) /Sequence="ATATTCAGCGGATTTCATGAAAGTTGCGCACCCTTACG" /Transferred_From="oDK_055" /Transferred_Similarity="100.00%" /label="oDK_055" primer_bind 1507..1528 /Sequence="GTAAGGGTGCGCAACTTTCATG" /Transferred_From="oMS_570" /Transferred_Similarity="100.00%" /label="oMS_570" inv_rep complement(1508..1519) /label="a1" cds 1526..2488 /locus_tag="ECD_00831" /codon_start=1 /transl_table=11 /product="retron EC86 RNA-directed DNA polymerase-like protein" /protein_id="ACT42726.1" /db_xref="GI:253977056" /label="retron EC86 RNA-directed DNA polymerase-like protein CDS" primer_bind 1526..1551 /Sequence="ATGAAATCCGCTGAATATTTGAACAC" /Transferred_From="oMS_533" /Transferred_Similarity="100.00%" /label="oMS_533" primer_bind 1588..1610 /Sequence="CAATTTGCATGACATGTCTAAGG" /Transferred_From="oMS_540" /Transferred_Similarity="100.00%" /label="oMS_540" primer_bind complement(1588..1610) /Sequence="CCTTAGACATGTCATGCAAATTG" /Transferred_From="oMS_525" /Transferred_Similarity="100.00%" /label="oMS_525" primer_bind complement(2464..2487) /Sequence="TAGGTCTTCGCTTTATTTAAAGGG" /Transferred_From="oMS_522" /Transferred_Similarity="100.00%" /label="oMS_522" primer_bind complement(2465..2486) /Sequence="AGGTCTTCGCTTTATTTAAAGG" /Transferred_From="oMS_559" /Transferred_Similarity="100.00%" /label="oMS_559" misc_feature 2488..2526 /label /note="Geneious type: Editing History Insertion" primer_bind 2504..2529 /Sequence="GTTTAACTTTAAGGAGGTTTGGAATG" /Transferred_From="oMS_523" /Transferred_Similarity="100.00%" /label="oMS_523" CDS 2527..3312 /label="beta" primer_bind complement(2598..2617) /Sequence="GAAGAGTGGTGATCAGTTCC" /Transferred_From="oMS_519" /Transferred_Similarity="100.00%" /label="oMS_519" primer_bind complement(2620..2640) /Sequence="ATCACCTTTAAATGCCGTCTG" /Transferred_From="oMS_524" /Transferred_Similarity="100.00%" /label="oMS_524" primer_bind complement(3294..3325) /Sequence="GGTCGACTCTAGATCATGCTGCCACCTTCTGC" /Transferred_From="oDK_062" /Transferred_Similarity="100.00%" /label="oDK_062" primer_bind complement(3294..3321) /Sequence="GACTCTAGATCATGCTGCCACCTTCTGC" /Transferred_From="oDK_047" /Transferred_Similarity="100.00%" /label="oDK_047" primer_bind complement(3375..3392) /Sequence="GATTTAATCTGTATCAGG" /Transferred_From="pBAD Reverse" /Transferred_Similarity="100.00%" /label="pBAD Reverse" terminator 3425..3582 /Imported_from="Pla sMapper" /X-Transferred_From="rrnB term" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_76 (pRSFRedGFP henry's v.nat plasmid)" /Transferred_Similarity="97.47%" /label="rrnB term" primer_bind 3525..3568 /Sequence="GCGAAATTTTGCCGACTGATCAAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0434" /X-Transferred_Similarity="65.91%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0434" primer_bind 3529..3568 /Sequence="GCGAAATTTTGCCGACTGAATAAAACGAAAGGCTCAGTCG" /X-Transferred_From="oMS_0433" /X-Transferred_Similarity="70.00%" /Transferred_From="pMS_31 (pAH_81 AY048720)" /Transferred_Similarity="100.00%" /label="oMS_0433" primer_bind 3548..3569 /Sequence="ATAAAACGAAAGGCTCAGTCGA" /Transferred_From="oMS_0388" /Transferred_Similarity="100.00%" /label="oMS_0388" primer_bind 4131..4157 /Sequence="AAGAACCTCAGATCCTTCCGTATTTAG" /Transferred_From="oMS_0460" /Transferred_Similarity="100.00%" /label="oMS_0460" CDS complement(4138..5088) /created_by="User" /label="repA101 CDS" primer_bind complement(4307..4327) /Sequence="CTACCTACGTAACGGACTAAG" /Transferred_From="repA_rev" /Transferred_Similarity="100.00%" /label="repA_rev" regulatory 5844..5949 /regulatory_class="terminator" /note="T0 terminator; terminator T0" /Transferred_From="KM521210, p ECG_MC3 KM521211 (mf-lon mcherry_pdt3)" /Transferred_Similarity="100.00%" /label="T0 terminator regulatory; terminator T0 regulatory" primer_bind 5921..5944 /Sequence="TTACTGGATCTATCAACAGGAGTC" /Transferred_From="oMS_0350" /Transferred_Similarity="100.00%" /label="oMS_0350" primer_bind complement(5967..5987) /Sequence="TGTCTAACAATTCGTTCAAGC" /Transferred_From="oMS_0337" /Transferred_Similarity="100.00%" /label="oMS_0337" misc_feature complement(5989..6775) /label="CmR" /note="Geneious type: Antibiotic Resistance" marker complement(5989..6648) /label="CAT marker" cds complement(5989..6648) /codon_start=1 /transl_table=11 /product="CmR" /protein_id="ADC42093.1" /db_xref="GI:288187151" /label="CmR CDS" primer_bind 6146..6167 /Sequence="GCGAAGAAGTTGTCCATATTGG" /Transferred_From="oMS_0383" /Transferred_Similarity="100.00%" /label="oMS_0383" primer_bind complement(6606..6632) /Sequence="CTGGATATACCACCGTTGATATATCCC" /Transferred_From="oMS_0382" /Transferred_Similarity="100.00%" /label="oMS_0382" primer_bind complement(6693..6714) /Sequence="GAAATAAGATCACTACCGGGCG" /Transferred_From="oMS_0222" /Transferred_Similarity="100.00%" /label="oMS_0222" primer_bind 6693..6714 /Sequence="CGCCCGGTAGTGATCTTATTTC" /Transferred_From="oMS_521" /Transferred_Similarity="100.00%" /label="oMS_521" ORIGIN 1 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc 61 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt 121 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 181 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 241 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 301 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 361 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 421 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 481 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 541 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 601 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 661 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 721 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 781 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 841 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 901 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 961 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 1021 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 1081 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 1141 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 1201 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 1261 ctactgtttc tccatacccg tttaattaaa tgcgcaccct tagcgagagg tttatcatta 1321 aggtcaacct ctggatgttg tttcggcatc ctgcattgaa tctgagttac tgtctgtttt 1381 cctgaattcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aattcaggaa acccgttttt 1501 tctgacgtaa gggtgcgcaa ctttcatgaa atccgctgaa tatttgaaca cttttagatt 1561 gagaaatctc ggcctacctg tcatgaacaa tttgcatgac atgtctaagg cgactcgcat 1621 atctgttgaa acacttcggt tgttaatcta tacagctgat tttcgctata ggatctacac 1681 tgtagaaaag aaaggcccag agaagagaat gagaaccatt taccaacctt ctcgagaact 1741 taaagcctta caaggatggg ttctacgtaa cattttagat aaactgtcgt catctccttt 1801 ttctattgga tttgaaaagc accaatctat tttgaataat gctaccccgc atattggggc 1861 aaactttata ctgaatattg atttggagga ttttttccca agtttaactg ctaacaaagt 1921 ttttggagtg ttccattctc ttggttataa tcgactaata tcttcagttt tgacaaaaat 1981 atgttgttat aaaaatctgc taccacaagg tgctccatca tcacctaaat tagctaatct 2041 aatatgttct aaacttgatt atcgtattca gggttatgca ggtagtcggg gcttgatata 2101 tacgagatat gccgatgacc tcaccttatc tgcacagtct atgaaaaagg ttgttaaagc 2161 acgtgatttt ttattttcta taatcccaag tgaaggattg gttattaact caaaaaaaac 2221 ttgtattagt gggcctcgta gtcagaggaa agttacaggt ttagttattt cacaagagaa 2281 agttgggata ggtagagaaa aatataaaga aattagagca aagatacatc atatattttg 2341 cggtaagtct tctgagatag aacacgttag gggatggttg tcatttattt taagtgtgga 2401 ttcaaaaagc cataggagat taataactta tattagcaaa ttagaaaaaa aatatggaaa 2461 gaacccttta aataaagcga agacctagga tcgaaataat tttgtttaac tttaaggagg 2521 tttggaatga gtactgcact cgcaacgctg gctgggaagc tggctgaacg tgtcggcatg 2581 gattctgtcg acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat 2641 gccagcgatg cgcagttcat cgcattactg atcgttgcca accagtacgg ccttaatccg 2701 tggacgaaag aaatttacgc ctttcctgat aagcagaatg gcatcgttcc ggtggtgggc 2761 gttgatggct ggtcccgcat catcaatgaa aaccagcagt ttgatggcat ggactttgag 2821 caggacaatg aatcctgtac atgccggatt taccgcaagg accgtaatca tccgatctgc 2881 gttaccgaat ggatggatga atgccgccgc gaaccattca aaactcgcga aggcagagaa 2941 atcacggggc cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag 3001 tgtgcccgtc tggccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt 3061 gtcgaaaata ctgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac 3121 gatgaaacca tgcaggagat taacactctg ctgatcgccc tggataaaac atgggatgac 3181 gacttattgc cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg 3241 acacaggccg aagcagtaaa agctcttgga ttcctgaaac agaaagccgc agagcagaag 3301 gtggcagcat gatctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 3361 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 3421 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 3481 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 3541 gcatcaaata aaacgaaagg ctcagtcgaa agactgaacg ctctcctgag taggacaaat 3601 ccgccgccct agacctaggg tacgggtttt gctgcccgca aacgggctgt tctggtgttg 3661 ctagtttgtt atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat 3721 ttcttccaga attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc 3781 ggcagctttg attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga 3841 gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt 3901 tcacccgttc tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg 3961 tgaacagctt tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca 4021 ccgttttcat ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt 4081 ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca 4141 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca 4201 tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa 4261 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg 4321 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt 4381 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt 4441 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc 4501 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc 4561 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt 4621 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa 4681 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa 4741 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca 4801 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag 4861 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg 4921 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt 4981 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata 5041 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg 5101 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt 5161 ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt 5221 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat 5281 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc 5341 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca 5401 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct 5461 cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc 5521 gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta 5581 aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa 5641 agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac 5701 tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc 5761 cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc 5821 ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa 5881 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg 5941 agtccaagcg agctcgatat ccgtcggctt gaacgaattg ttagacaatt acgccccgcc 6001 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc 6061 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata 6121 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc 6181 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc 6241 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag 6301 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc 6361 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat 6421 tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg 6481 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac 6541 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg 6601 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt 6661 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg 6721 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcg // LOCUS pBAD>_retron,_RT,_CspRecT_(sc101_cat) 8556 bp DNA circular UNA 29-FEB-2020 DEFINITION Gibson Assembly of mutL* and terminator (from 291) into pMS_369 extraction no rrnB. ACCESSION urn.local...5j-bmf2owc VERSION urn.local...5j-bmf2owc KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers terminator complement(1..85) /created_by="User" /label="araC native terminator" primer_bind 1..20 /Sequence="AATGTGCCTGTCAAATGGAC" /Transferred_From="oDK_052" /Transferred_Similarity="100.00%" /label="oDK_052" cds complement(86..1015) /codon_start=1 /transl_table=11 /product="araC" /protein_id="AIU94532.1" /Transferred_Translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSF NAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILL FPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFS DLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDH LADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIA TVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" /label="araC CDS" gene complement(86..964) /Imported_from="Pla sMapper" /X-Transferred_From="AraC" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraC" misc_feature 978..>1281 /created_by="User" /modified_by="User" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="segment of pBAD_33" regulatory 991..1276 /regulatory_class="promoter" /note="PBAD promoter" /Transferred_From="pECA102 KM521212" /Transferred_Similarity="100.00%" misc_feature 993..1010 /note="misc_feature; operator O2" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" misc_feature 994..1009 /Imported_from="Pla sMapper" /X-Transferred_From="araO2 reg; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="araO2 reg" /note="Geneious type: regulatory sequence" primer_bind 1088..1115 /Sequence="CATTCTGTAACAAAGCGGGACCAAAGCC" /Transferred_From="oMS_818" /Transferred_Similarity="100.00%" /label="oMS_818" promoter complement(1115..1143) /note="Pc promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; Pc promoter" misc_feature complement(1115..1143) /ApEinfo_revcolor="#b7e6d7" /ApEinfo_fwdcolor="#b7e6d7" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="Pc" primer_bind 1119..1140 /Sequence="ACAAAAACGCGTAACAAAAGTG" /Transferred_From="oMS_802" /Transferred_Similarity="100.00%" /label="oMS_802" primer_bind 1148..1166 /Sequence="TCACGGCAGAAAAGTCCAC" /Transferred_From="oMS_806" /Transferred_Similarity="100.00%" /label="oMS_806" misc_feature 1151..1172 /note="misc_feature; operator O1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" gene 1203..1241 /Imported_from="Pla sMapper" /X-Transferred_From="AraI1I2; pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="AraI1I2" misc_feature 1203..1241 /note="misc_feature; operator I2 + I1" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" primer_bind 1213..1272 /Sequence="TATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTC TCTACTGTTTCTC" /Transferred_From="oMS_808" /Transferred_Similarity="100.00%" /label="oMS_808" promoter 1238..1266 /Imported_from="Pla sMapper" /X-Transferred_From="ARA prom" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ARA prom" promoter 1240..1267 /note="PBAD promoter; promoter" /Transferred_From="pMS_39 (pTB273) pARA bla p21 plasmid (modified)" /Transferred_Similarity="100.00%" /label="promoter; PBAD promoter" misc_feature 1240..1267 /ApEinfo_revcolor="#ff9ccd" /ApEinfo_fwdcolor="#ff9ccd" /X-Transferred_From="pMS_118 (pKD46)" /X-Transferred_Similarity="100.00%" /Transferred_From="pMS_116 (pMA7CR_2.0), pMS_118 (pKD46)" /Transferred_Similarity="100.00%" /label="ParaB" primer_bind complement(1257..1281) /Sequence="ACGGGTATGGAGAAACAGTAGAGAG" /Transferred_From="oMS_801" /Transferred_Similarity="100.00%" /label="oMS_801" misc_feature 1289..1290 /label="AAGAATTCATTAAAGAGGAGAAAGGTACCTAGCTATGTTAGGAGCGTCTT TGATAGAAAGACACTTGACCGATTGCGGCTTGAGATTATGAATTTTGAAAACCGTAGA AAATCAACATTTAACTATGATAAGATTCCGT" /note="Geneious type: Editing History Deletion" misc_RNA 1290..1523 /product="msr" /function="primer for the Ec86 RT" /modified_by="Max_Schubert" /label="msr86" inv_rep 1291..1302 /label="a2" Branched_G 1303 primer_bind 1328..1348 /Sequence="CCTCTGGATGTTGTTTCGGCA" /Transferred_From="oMS_0409" /Transferred_Similarity="100.00%" /label="oMS_0409" primer_bind complement(1329..1348) /Sequence="TGCCGAAACAACATCCAGAG" /Transferred_From="oMS_539" /Transferred_Similarity="100.00%" /label="oMS_539" primer_bind 1350..1371 /Sequence="CCTGCATTGAATCTGAGTTACT" /Transferred_From="oMS_545" /Transferred_Similarity="100.00%" /label="oMS_545" msd complement(1361..1506) /created_by="Max_Schubert" primer_bind 1361..1389 /Sequence="TCTGAGTTACTGTCTGTTTTCCTGAATTC" /Transferred_From="oMS_0416" /Transferred_Similarity="100.00%" /label="oMS_0416" primer_bind complement(1362..1383) /Sequence="AGGAAAACAGACAGTAACTCAG" /Transferred_From="oMS_532" /Transferred_Similarity="100.00%" /label="oMS_532" primer_bind complement(1366..1389) /Sequence="GAATTCAGGAAAACAGACAGTAAC" /Transferred_From="oMS_486" /Transferred_Similarity="100.00%" /label="oMS_486" misc_feature 1390..1479 /Original_Bases="TGTCGTTGGTGACGTAATCGGTAAATACCATCCCCATGGTG ACTTGGCCGTCTATGACACGATCGTCCGCATGGCGCAGCCATTCTCGCT" /label="TGTCGTTGGTGACGTAATCGGTAAATACCATCCCCATGGTGACTTGGCCG TCTATGACACGATCGTCCGCATGGCGCAGCCATTCTCGCT" /note="Geneious type: Editing History Replacement" misc_feature complement(1390..1479) /created_by="Max_Schubert" /label="insert donor here (reverse orientation)" primer_bind complement(1480..1506) /Sequence="GTCAGAAAAAACGGGTTTCCTGAATTC" /Transferred_From="oMS_0417" /Transferred_Similarity="100.00%" /label="oMS_0417" primer_bind 1480..1502 /Sequence="GAATTCAGGAAACCCGTTTTTTC" /Transferred_From="oMS_487" /Transferred_Similarity="100.00%" /label="oMS_487" primer_bind 1485..1513 /Sequence="CAGGAAACCCGTTTTTTCTGACGTAAGGG" /Transferred_From="oMS_623" /Transferred_Similarity="100.00%" /label="oMS_623" primer_bind complement(1502..1519) /Sequence="TGCGCACCCTTACGTCAG" /Transferred_From="oMS_757" /Transferred_Similarity="100.00%" /label="oMS_757" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_668" /Transferred_Similarity="100.00%" /label="oMS_668" primer_bind complement(1504..1523) /Sequence="AAGTTGCGCACCCTTACGTC" /Transferred_From="oMS_696" /Transferred_Similarity="100.00%" /label="oMS_696" primer_bind 1507..1528 /Sequence="GTAAGGGTGCGCAACTTTCATG" /Transferred_From="oMS_570" /Transferred_Similarity="100.00%" /label="oMS_570" inv_rep complement(1508..1519) /label="a1" cds 1526..2488 /locus_tag="ECD_00831" /codon_start=1 /transl_table=11 /product="retron EC86 RNA-directed DNA polymerase-like protein" /protein_id="ACT42726.1" /db_xref="GI:253977056" /label="retron EC86 RNA-directed DNA polymerase-like protein CDS" primer_bind 1526..1551 /Sequence="ATGAAATCCGCTGAATATTTGAACAC" /Transferred_From="oMS_533" /Transferred_Similarity="100.00%" /label="oMS_533" primer_bind 1588..1610 /Sequence="CAATTTGCATGACATGTCTAAGG" /Transferred_From="oMS_540" /Transferred_Similarity="100.00%" /label="oMS_540" primer_bind complement(1588..1610) /Sequence="CCTTAGACATGTCATGCAAATTG" /Transferred_From="oMS_525" /Transferred_Similarity="100.00%" /label="oMS_525" misc_feature 2119 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_difference 2119 /created_by="User" /label="synonymous mutation?" primer_bind complement(2464..2487) /Sequence="TAGGTCTTCGCTTTATTTAAAGGG" /Transferred_From="oMS_522" /Transferred_Similarity="100.00%" /label="oMS_522" primer_bind complement(2465..2486) /Sequence="AGGTCTTCGCTTTATTTAAAGG" /Transferred_From="oMS_559" /Transferred_Similarity="100.00%" /label="oMS_559" misc_difference complement(2489..2520) /created_by="User" /label=933 gene 2527..3339 /created_by="Tim" /modified_by="Max_Schubert" /label="CspRecT" unsure complement(2820..2828) /created_by="User" /label="dnaA box" misc_feature 3367..5214 /note="mutL E32K" terminator 5215..5340 /created_by="Max_Schubert" /modified_by="Max_Schubert" /label="native terminator of lambda-RED" unsure complement(5344..5352) /created_by="User" /label="dnaA box" primer_bind 5912..5938 /Sequence="AAGAACCTCAGATCCTTCCGTATTTAG" /Transferred_From="oMS_0460" /Transferred_Similarity="100.00%" /label="oMS_0460" CDS complement(5919..6869) /created_by="User" /label="repA101 CDS" primer_bind complement(6062..6094) /Sequence="GACTAAGAAAAACACTACACGATGCTTTAACTG" /Transferred_From="oMS_813" /Transferred_Similarity="100.00%" /label="oMS_813" primer_bind 6095..6124 /Sequence="CGTTACGTAGGTAGGAATCTGATGTAATGG" /Transferred_From="oMS_812" /Transferred_Similarity="100.00%" /label="oMS_812" primer_bind 6154..6175 /Sequence="ATCTGGTTGTTCTCAAGTTCGG" /Transferred_From="oMS_918" /Transferred_Similarity="100.00%" /label="oMS_918" primer_bind 6594..6618 /Sequence="AAAGCCTTTAACCAAAGGATTCCTG" /Transferred_From="oMS_920" /Transferred_Similarity="100.00%" /label="oMS_920" primer_bind complement(6594..6618) /Sequence="CAGGAATCCTTTGGTTAAAGGCTTT" /Transferred_From="oMS_916" /Transferred_Similarity="100.00%" /label="oMS_916" unsure complement(6871..7127) /created_by="User" /label="origin_stuff" primer_bind complement(6961..6983) /Sequence="GTCTAGCAGAATTTACAGATACC" /Transferred_From="oMS_919" /Transferred_Similarity="100.00%" /label="oMS_919" primer_bind complement(7017..7037) /Sequence="GGTCTAGCGGAATTTACAGAG" /Transferred_From="oMS_917" /Transferred_Similarity="100.00%" /label="oMS_917" unsure complement(7128..7136) /created_by="User" /label="dnaA box" regulatory 7625..7730 /regulatory_class="terminator" /note="T0 terminator; terminator T0" /Transferred_From="KM521210, p ECG_MC3 KM521211 (mf-lon mcherry_pdt3)" /Transferred_Similarity="100.00%" /label="T0 terminator regulatory; terminator T0 regulatory" primer_bind 7702..7725 /Sequence="TTACTGGATCTATCAACAGGAGTC" /Transferred_From="oMS_0350" /Transferred_Similarity="100.00%" /label="oMS_0350" primer_bind complement(7746..7778) /Sequence="GGGGCGTAATTGTCTAACAATTCGTTCAAGCCG" /Transferred_From="oDK_049" /Transferred_Similarity="100.00%" /label="oDK_049" primer_bind complement(7748..7768) /Sequence="TGTCTAACAATTCGTTCAAGC" /Transferred_From="oMS_0337" /Transferred_Similarity="100.00%" /label="oMS_0337" primer_bind 7761..7785 /Sequence="GTTAGACAATTACGCCCCGCCCTGC" /Transferred_From="oDK_050" /Transferred_Similarity="100.00%" /label="oDK_050" misc_feature complement(7770..8556) /label="CmR" /note="Geneious type: Antibiotic Resistance" marker complement(7770..8429) /label="CAT marker" cds complement(7770..8429) /codon_start=1 /transl_table=11 /product="CmR" /protein_id="ADC42093.1" /db_xref="GI:288187151" /label="CmR CDS" primer_bind 7927..7948 /Sequence="GCGAAGAAGTTGTCCATATTGG" /Transferred_From="oMS_0383" /Transferred_Similarity="100.00%" /label="oMS_0383" unsure complement(8246..8254) /created_by="User" /label="dnaA box" primer_bind complement(8387..8413) /Sequence="CTGGATATACCACCGTTGATATATCCC" /Transferred_From="oMS_0382" /Transferred_Similarity="100.00%" /label="oMS_0382" primer_bind complement(8474..8495) /Sequence="GAAATAAGATCACTACCGGGCG" /Transferred_From="oMS_0222" /Transferred_Similarity="100.00%" /label="oMS_0222" primer_bind 8474..8495 /Sequence="CGCCCGGTAGTGATCTTATTTC" /Transferred_From="oMS_521" /Transferred_Similarity="100.00%" /label="oMS_521" ORIGIN 1 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc 61 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt 121 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg 181 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg 241 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc 301 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct 361 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag 421 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc 481 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt 541 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg 601 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg 661 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt 721 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc 781 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca 841 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac 901 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag 961 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac 1021 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta 1081 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 1141 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 1201 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 1261 ctactgtttc tccatacccg tttaattaaa tgcgcaccct tagcgagagg tttatcatta 1321 aggtcaacct ctggatgttg tttcggcatc ctgcattgaa tctgagttac tgtctgtttt 1381 cctgaattcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng aattcaggaa acccgttttt 1501 tctgacgtaa gggtgcgcaa ctttcatgaa atccgctgaa tatttgaaca cttttagatt 1561 gagaaatctc ggcctacctg tcatgaacaa tttgcatgac atgtctaagg cgactcgcat 1621 atctgttgaa acacttcggt tgttaatcta tacagctgat tttcgctata ggatctacac 1681 tgtagaaaag aaaggcccag agaagagaat gagaaccatt taccaacctt ctcgagaact 1741 taaagcctta caaggatggg ttctacgtaa cattttagat aaactgtcgt catctccttt 1801 ttctattgga tttgaaaagc accaatctat tttgaataat gctaccccgc atattggggc 1861 aaactttata ctgaatattg atttggagga ttttttccca agtttaactg ctaacaaagt 1921 ttttggagtg ttccattctc ttggttataa tcgactaata tcttcagttt tgacaaaaat 1981 atgttgttat aaaaatctgc taccacaagg tgctccatca tcacctaaat tagctaatct 2041 aatatgttct aaacttgatt atcgtattca gggttatgca ggtagtcggg gcttgatata 2101 tacgagatat gccgatgacc tcaccttatc tgcacagtct atgaaaaagg ttgttaaagc 2161 acgtgatttt ttattttcta taatcccaag tgaaggattg gttattaact caaaaaaaac 2221 ttgtattagt gggcctcgta gtcagaggaa agttacaggt ttagttattt cacaagagaa 2281 agttgggata ggtagagaaa aatataaaga aattagagca aagatacatc atatattttg 2341 cggtaagtct tctgagatag aacacgttag gggatggttg tcatttattt taagtgtgga 2401 ttcaaaaagc cataggagat taataactta tattagcaaa ttagaaaaaa aatatggaaa 2461 gaacccttta aataaagcga agacctagga tcgaaataat tttgtttaac tttaaggagg 2521 tttggaatga accaaatcgt gaagttcact gacgactctg gcctggcggt tcaagttact 2581 ccagacgatg ttcgccgtta tatctgtgag aacgctactg aaaaagaggt gggcctcttt 2641 ctgcaactct gtcagactca acgtctgaat ccgtttgtga aagacgctta cctggtgaaa 2701 tacggcggtg ctccagcttc tatgattact tcctatcaag tttttaaccg tcgcgcgtgt 2761 cgtgatgcta actatgatgg tatcaaatct ggtgtggttg ttctgcgtga cggtgatgtt 2821 gtgcataaac gtggtgctgc gtgctacaaa aaggcgggtg aggagctcat cggtggttgg 2881 gcggaagttc gctttaagga tggccgcgag actgcgtatg ctgaggtggc gctcgacgac 2941 tattccaccg gcaaatctaa ttgggcgaaa atgccgggtg ttatgatcga aaaatgcgcg 3001 aaggctgctg cttggcgcct cgcgttcccg gacacttttc agggcatgta cgctgcggag 3061 gaaatggatc aagcgcaaca gccagaacag gtgcgcgctc aggcggagca accagtggat 3121 ctccagccaa tccgcgaact cttcaagcca tattgcgaac acttcggcat cactccggct 3181 gagggtatga ctgctgtttg tggtgcggtg ggcgctgaag gcatgcactc tatgaccgag 3241 cagcaagctc gccgtgctcg cgcttggatg gaggaagaaa tggctgcgcc agctgtggaa 3301 gcggagtatg aggttgttga cgagggcgag gtgttttaaa tggcgatgag agaggaggat 3361 tacaaaatgc caattcaggt cttaccgcca caactggcga accagattgc cgcaggtgag 3421 gtggtcgagc gacctgcgtc ggtagtcaaa gaactagtga aaaacagcct cgatgcaggt 3481 gcgacgcgta tcgatattga tatcgaacgc ggtggggcga aacttatccg cattcgtgat 3541 aacggctgcg gtatcaaaaa agatgagctg gcgctggcgc tggctcgtca tgccaccagt 3601 aaaatcgcct ctctggacga tctcgaagcc attatcagcc tgggctttcg cggtgaggcg 3661 ctggcgagta tcagttcggt ttcccgcctg acgctcactt cacgcaccgc agaacagcag 3721 gaagcctggc aggcctatgc cgaagggcgc gatatgaacg tgacggtaaa accggcggcg 3781 catcctgtgg ggacgacgct ggaggtgctg gatctgttct acaacacccc ggcgcggcgc 3841 aaattcctgc gcaccgagaa aaccgaattt aaccacattg atgagatcat ccgccgcatt 3901 gcgctggcgc gtttcgacgt cacgatcaac ctgtcgcata acggtaaaat tgtgcgtcag 3961 taccgcgcag tgccggaagg cgggcaaaaa gaacggcgct taggcgcgat ttgcggcacc 4021 gcttttcttg aacaagcgct ggcgattgaa tggcaacacg gcgatctcac gctacgcggc 4081 tgggtggccg atccaaatca caccacgccc gcactggcag aaattcagta ttgctacgtg 4141 aacggtcgca tgatgcgcga tcgcctgatc aatcacgcga tccgccaggc ctgcgaagac 4201 aaactggggg ccgatcagca accggcattt gtgttgtatc tggagatcga cccacatcag 4261 gtggacgtca acgtgcaccc cgccaaacac gaagtgcgtt tccatcagtc gcgtctggtg 4321 catgatttta tctatcaggg cgtgctgagc gtgctacaac agcaactgga aacgccgcta 4381 ccgctggacg atgaacccca acctgcaccg cgttccattc cggaaaaccg cgtggcggcg 4441 gggcgcaatc actttgcaga accggcagct cgtgagccgg tagctccgcg ctacactcct 4501 gcgccagcat caggcagtcg tccggctgcc ccctggccga atgcgcagcc aggctaccag 4561 aaacagcaag gtgaagtgta tcgccagctt ttgcaaacgc ccgcgccgat gcaaaaatta 4621 aaagcgccgg aaccgcagga acctgcactt gcggcgaaca gtcagagttt tggtcgggta 4681 ctgactatcg tccattccga ctgtgcgttg ctggagcgcg acggcaacat ttcactttta 4741 tccttgccag tggcagaacg ttggctgcgt caggcacaat tgacgccggg tgaagcgccc 4801 gtttgcgccc agccgctgct gattccgttg cggctaaaag tttctgccga agaaaaatcg 4861 gcattagaaa aagcgcagtc tgccctggcg gaattgggta ttgatttcca gtcagatgca 4921 cagcatgtga ccatcagggc agtgccttta cccttacgcc aacaaaattt acaaatcttg 4981 attcctgaac tgataggcta cctggcgaag cagtccgtat tcgaacctgg caatattgcg 5041 cagtggattg cacgaaatct gatgagcgaa catgcgcagt ggtcaatggc acaggccata 5101 accctgctgg cggacgtgga acggttatgt ccgcaacttg tgaaaacgcc gccgggtggt 5161 ctgttacaat ctgttgattt acatccggcg ataaaagccc tgaaagatga gtgaaatggc 5221 gatgagcatc ctcacgataa tatccgggta ggcgcaatca ctttcgtcta ctccgttaca 5281 aagcgaggct gggtatttcc cggcctttct gttatccgaa atccactgaa agcacagcgg 5341 agatgtgtat aagagacagc tgcctcctga gtaggacaaa tccgccgccc tagacctagg 5401 gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc 5461 gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag aattgccatg 5521 attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa 5581 gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca 5641 ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacccgtt ctattaggtg 5701 ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac 5761 caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata 5821 tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg tttgttagtc 5881 ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc 5941 agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga 6001 tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt 6061 gcagttaaag catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta 6121 atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg 6181 agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc 6241 ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa 6301 acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta 6361 aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt 6421 aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga 6481 ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact 6541 aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct 6601 ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct 6661 aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg 6721 aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca 6781 cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca 6841 tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca 6901 ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct ttgagttgtg 6961 ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct 7021 gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat 7081 agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca 7141 ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta 7201 caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg 7261 ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat 7321 tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct 7381 tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc 7441 agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt 7501 cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac 7561 tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc 7621 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat 7681 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata 7741 tccgtcggct tgaacgaatt gttagacaat tacgccccgc cctgccactc atcgcagtac 7801 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc 7861 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa 7921 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc 7981 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg 8041 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg 8101 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa 8161 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga 8221 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt 8281 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat 8341 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg 8401 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat 8461 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt 8521 acgtgccgat caacgtctca ttttcgccag atatcg //