srr titles t.data.frame(identifiers_SRX) PRIMARY_ID specie PAIRED dates study_title 1 2 nreads read_length unlist(library_selection) unlist(library_source) study_abstract A C G T N age biomaterial_provider BioSampleModel isolate sex tissue text SRR7294023 single-cell RNA-seq of human adipose-derived mesenchymal stem cells SRX4119296 SRS3334255 Homo sapiens NA NA single-cell transcriptomic sequencing of 24,370 adipose-derived mesenchymal stem cells SRP148833 PRJNA472816 176545104 149.75 other TRANSCRIPTOMIC Adipose-derived mesenchymal stem cells (ADSCs) show considerable promise for clinical applications in regenerative medicine. However, our understanding of the intrapopulation heterogeneity and the pattern of lineage priming of cultured ADSCs has been impeded by technical challenges. The lack of a thorough understanding of the cellular heterogeneity of ADSCs has hampered the development of an efficient and reproducible clinical application. Single-cell RNA-seq gives us an unprecedented chance to understand these aspects of ADSCs in great detail. Here, we provide the first comprehensive study of the cellular heterogeneity and lineage priming in cultured ADSCs by large-scale single-cell transcriptomic sequencing. We aim to 1) dissect the heterogeneity of ADSCs in an unbiased manner, 2) unravel the pattern of lineage priming at a single-cell resolution, and 3) test the hypothesis that cell cycle phase affects cellular response to differentiation cues in multipotent stem cells. 15114165365 8753986704 10204307695 18840554730 2208689 44 Division of Plastic Surgery, Peking Union Medical College Hospital Human N8 female adipose tissue text.1 SRR7253635 single-cell RNA-seq of human adipose-derived mesenchymal stem cells SRX4119295 SRS3334253 Homo sapiens NA NA single-cell transcriptomic sequencing of 24,370 adipose-derived mesenchymal stem cells SRP148833 PRJNA472816 265719530 149.99 other TRANSCRIPTOMIC Adipose-derived mesenchymal stem cells (ADSCs) show considerable promise for clinical applications in regenerative medicine. However, our understanding of the intrapopulation heterogeneity and the pattern of lineage priming of cultured ADSCs has been impeded by technical challenges. The lack of a thorough understanding of the cellular heterogeneity of ADSCs has hampered the development of an efficient and reproducible clinical application. Single-cell RNA-seq gives us an unprecedented chance to understand these aspects of ADSCs in great detail. Here, we provide the first comprehensive study of the cellular heterogeneity and lineage priming in cultured ADSCs by large-scale single-cell transcriptomic sequencing. We aim to 1) dissect the heterogeneity of ADSCs in an unbiased manner, 2) unravel the pattern of lineage priming at a single-cell resolution, and 3) test the hypothesis that cell cycle phase affects cellular response to differentiation cues in multipotent stem cells. 23360092898 13013282504 14827553787 28504519256 296954 25 Division of Plastic Surgery, Peking Union Medical College Hospital Human N5 female adipose tissue text.2 SRR7363187 single-cell RNA-seq of human adipose-derived mesenchymal stem cells SRX4119294 SRS3334254 Homo sapiens NA NA single-cell transcriptomic sequencing of 24,370 adipose-derived mesenchymal stem cells SRP148833 PRJNA472816 352873537 149.98 other TRANSCRIPTOMIC Adipose-derived mesenchymal stem cells (ADSCs) show considerable promise for clinical applications in regenerative medicine. However, our understanding of the intrapopulation heterogeneity and the pattern of lineage priming of cultured ADSCs has been impeded by technical challenges. The lack of a thorough understanding of the cellular heterogeneity of ADSCs has hampered the development of an efficient and reproducible clinical application. Single-cell RNA-seq gives us an unprecedented chance to understand these aspects of ADSCs in great detail. Here, we provide the first comprehensive study of the cellular heterogeneity and lineage priming in cultured ADSCs by large-scale single-cell transcriptomic sequencing. We aim to 1) dissect the heterogeneity of ADSCs in an unbiased manner, 2) unravel the pattern of lineage priming at a single-cell resolution, and 3) test the hypothesis that cell cycle phase affects cellular response to differentiation cues in multipotent stem cells. 32070054339 17044939983 18469441126 38259345286 610284 28 Division of Plastic Surgery, Peking Union Medical College Hospital Human N7 female adipose tissue