"Supplementary Data File 3: Location and annotation of single nucleotide polymorphisms (SNPs) across the mitogenome of the Atlantic Puffin detected by sequencing 71 individuals across 12 colonies. Genotypes were jointly called with GATK v4.1.4 by using the HaplotypeCaller, CombineGVCFs and GenotypeGVCFs tool. Genotypes were filtered with BCFtools v1.9 according to GATKs Best Practices. Indels and non-biallelic SNPs were removed and only SNPs present in all individuals were kept. ",,,,,,, Position,Reference,Alternate,Annotation,Gene Name,Amino Acid Change,, 56,T,C,non_coding_transcript_variant,trnF,,, 209,A,G,non_coding_transcript_variant,rrnS,,, 241,C,T,non_coding_transcript_variant,rrnS,,, 342,A,G,non_coding_transcript_variant,rrnS,,, 437,T,C,non_coding_transcript_variant,rrnS,,, 500,C,T,non_coding_transcript_variant,rrnS,,, 857,C,T,non_coding_transcript_variant,rrnS,,, 888,T,C,non_coding_transcript_variant,rrnS,,, 963,C,T,non_coding_transcript_variant,rrnS,,, 979,C,T,non_coding_transcript_variant,rrnS,,, 1128,A,G,non_coding_transcript_variant,rrnL,,, 1247,C,T,non_coding_transcript_variant,rrnL,,, 1511,C,T,non_coding_transcript_variant,rrnL,,, 1850,T,C,non_coding_transcript_variant,rrnL,,, 2268,G,A,non_coding_transcript_variant,rrnL,,, 2285,C,T,non_coding_transcript_variant,rrnL,,, 2303,G,A,non_coding_transcript_variant,rrnL,,, 2480,T,C,non_coding_transcript_variant,rrnL,,, 2583,A,G,non_coding_transcript_variant,rrnL,,, 2657,T,C,non_coding_transcript_variant,rrnL,,, 3273,A,G,missense_variant,nad1,p.Ile161Val,, 3284,C,T,synonymous_variant,nad1,p.Ser164Ser,, 3317,C,T,synonymous_variant,nad1,p.Thr175Thr,, 3323,G,A,synonymous_variant,nad1,p.Glu177Glu,, 3416,G,A,synonymous_variant,nad1,p.Thr208Thr,, 3599,A,G,synonymous_variant,nad1,p.Lys269Lys,, 3618,G,A,missense_variant,nad1,p.Gly276Ser,, 3849,A,G,intergenic_region,trnI-trnQ,,, 4044,A,G,missense_variant,nad2,p.Ile19Val,, 4115,C,A,synonymous_variant,nad2,p.Pro42Pro,, 4274,C,T,synonymous_variant,nad2,p.Cys95Cys,, 4418,C,T,synonymous_variant,nad2,p.Phe143Phe,, 4472,A,C,synonymous_variant,nad2,p.Ser161Ser,, 4491,A,G,missense_variant,nad2,p.Met168Val,, 4550,G,A,synonymous_variant,nad2,p.Leu187Leu,, 4559,G,A,synonymous_variant,nad2,p.Met190Met,, 4573,T,C,missense_variant,nad2,p.Ile195Thr,, 4608,A,G,missense_variant,nad2,p.Ile207Val,, 4760,C,T,synonymous_variant,nad2,p.Leu257Leu,, 4788,A,G,missense_variant,nad2,p.Ile267Val,, 5187,C,T,non_coding_transcript_variant,trnN,,, 5483,C,T,synonymous_variant,cox1,p.Thr32Thr,, 5700,C,T,synonymous_variant,cox1,p.Leu105Leu,, 5702,A,G,synonymous_variant,cox1,p.Leu105Leu,, 5723,G,A,synonymous_variant,cox1,p.Leu112Leu,, 5753,A,G,synonymous_variant,cox1,p.Gly122Gly,, 5759,T,C,synonymous_variant,cox1,p.Gly124Gly,, 5804,C,T,synonymous_variant,cox1,p.His139His,, 5972,A,G,synonymous_variant,cox1,p.Leu195Leu,, 5996,C,T,synonymous_variant,cox1,p.Leu203Leu,, 5999,T,C,synonymous_variant,cox1,p.Ala204Ala,, 6035,A,G,synonymous_variant,cox1,p.Leu216Leu,, 6146,G,A,synonymous_variant,cox1,p.Gly253Gly,, 6221,G,A,synonymous_variant,cox1,p.Met278Met,, 6222,C,T,synonymous_variant,cox1,p.Leu279Leu,, 6242,A,G,synonymous_variant,cox1,p.Gly285Gly,, 6299,T,G,synonymous_variant,cox1,p.Ala304Ala,, 6326,T,C,synonymous_variant,cox1,p.Ile313Ile,, 6362,C,T,synonymous_variant,cox1,p.Leu325Leu,, 6569,T,C,synonymous_variant,cox1,p.Phe394Phe,, 6585,T,C,synonymous_variant,cox1,p.Leu400Leu,, 6674,G,A,synonymous_variant,cox1,p.Gln429Gln,, 6836,A,T,synonymous_variant,cox1,p.Val483Val,, 6891,C,T,missense_variant,cox1,p.Pro502Ser,, 6990,C,T,non_coding_transcript_variant,trnS2,,, 7222,A,G,synonymous_variant,cox2,p.Lys49Lys,, 7300,T,C,synonymous_variant,cox2,p.Leu75Leu,, 7440,T,C,missense_variant,cox2,p.Ile122Thr,, 7453,G,A,synonymous_variant,cox2,p.Glu126Glu,, 7499,G,A,missense_variant,cox2,p.Val142Ile,, 7525,C,T,synonymous_variant,cox2,p.Arg150Arg,, 7543,C,T,synonymous_variant,cox2,p.Asp156Asp,, 7564,T,C,synonymous_variant,cox2,p.Ala163Ala,, 7579,G,A,synonymous_variant,cox2,p.Gly168Gly,, 7606,A,G,synonymous_variant,cox2,p.Arg177Arg,, 7939,G,A,synonymous_variant,atp8,p.Leu36Leu,, 7965,T,C,missense_variant,atp8,p.Ile45Thr,, 8026,T,C,missense_variant,atp6,p.Tyr13His,, 8067,T,C,synonymous_variant,atp6,p.Phe26Phe,, 8289,C,T,synonymous_variant,atp6,p.Ser100Ser,, 8309,T,C,missense_variant,atp6,p.Phe107Ser,, 8370,C,T,synonymous_variant,atp6,p.Gly127Gly,, 8546,T,C,missense_variant,atp6,p.Ile186Thr,, 8622,G,A,synonymous_variant,atp6,p.Gln211Gln,, 8738,C,T,synonymous_variant,cox3,p.Ala22Ala,, 8765,A,G,synonymous_variant,cox3,p.Leu31Leu,, 8813,G,A,synonymous_variant,cox3,p.Leu47Leu,, 9026,T,C,synonymous_variant,cox3,p.Pro118Pro,, 9041,C,T,synonymous_variant,cox3,p.Pro123Pro,, 9096,G,A,missense_variant,cox3,p.Val142Ile,, 9113,G,A,synonymous_variant,cox3,p.Ala147Ala,, 9170,G,A,synonymous_variant,cox3,p.Thr166Thr,, 9254,G,A,synonymous_variant,cox3,p.Gly194Gly,, 9294,G,A,missense_variant,cox3,p.Val208Ile,, 9320,G,A,synonymous_variant,cox3,p.Ser216Ser,, 9615,G,A,synonymous_variant,nad3,p.Pro25Pro,, 9627,G,A,synonymous_variant,nad3,p.Lys29Lys,, 9874,A,G,splice_region_variant&synonymous_variant,nad3,p.???112???,, 10004,T,C,synonymous_variant,nad4l,p.Leu19Leu,, 10017,G,A,missense_variant,nad4l,p.Arg23Gln,, 10045,C,T,synonymous_variant,nad4l,p.Cys32Cys,, 10468,A,G,missense_variant,nad4,p.Met77Val,, 10500,G,A,synonymous_variant,nad4,p.Glu87Glu,, 10506,G,A,synonymous_variant,nad4,p.Leu89Leu,, 10507,A,G,missense_variant,nad4,p.Thr90Ala,, 10528,C,A,missense_variant,nad4,p.Leu97Met,, 10716,G,A,synonymous_variant,nad4,p.Pro159Pro,, 10726,G,A,missense_variant,nad4,p.Ala163Thr,, 10737,T,C,synonymous_variant,nad4,p.Tyr166Tyr,, 10744,A,G,missense_variant,nad4,p.Thr169Ala,, 10824,G,A,synonymous_variant,nad4,p.Ser195Ser,, 11079,T,C,synonymous_variant,nad4,p.Thr280Thr,, 11082,T,C,synonymous_variant,nad4,p.Asp281Asp,, 11223,T,C,synonymous_variant,nad4,p.Cys328Cys,, 11224,C,T,synonymous_variant,nad4,p.Leu329Leu,, 11247,T,C,synonymous_variant,nad4,p.Arg336Arg,, 11337,T,C,synonymous_variant,nad4,p.Asn366Asn,, 11448,T,C,synonymous_variant,nad4,p.Thr403Thr,, 11460,C,T,synonymous_variant,nad4,p.Thr407Thr,, 11509,G,A,missense_variant,nad4,p.Ala424Thr,, 11511,A,G,synonymous_variant,nad4,p.Ala424Ala,, 11697,T,C,non_coding_transcript_variant,trnS1,,, 11829,G,A,missense_variant,nad5,p.Ala10Thr,, 11831,T,C,synonymous_variant,nad5,p.Ala10Ala,, 11964,G,A,missense_variant,nad5,p.Ala55Thr,, 11978,G,A,synonymous_variant,nad5,p.Pro59Pro,, 12008,G,A,synonymous_variant,nad5,p.Glu69Glu,, 12058,C,T,missense_variant,nad5,p.Pro86Leu,, 12060,G,A,missense_variant,nad5,p.Val87Ile,, 12086,G,A,synonymous_variant,nad5,p.Ser95Ser,, 12110,A,T,synonymous_variant,nad5,p.Leu103Leu,, 12237,C,A,missense_variant,nad5,p.Leu146Met,, 12315,G,A,missense_variant,nad5,p.Glu172Lys,, 12320,C,T,synonymous_variant,nad5,p.Ala173Ala,, 12368,T,C,synonymous_variant,nad5,p.Ile189Ile,, 12527,C,T,synonymous_variant,nad5,p.Pro242Pro,, 12784,A,G,missense_variant,nad5,p.Asn328Ser,, 12798,G,A,missense_variant,nad5,p.Ala333Thr,, 12839,G,A,synonymous_variant,nad5,p.Met346Met,, 12857,A,G,synonymous_variant,nad5,p.Gly352Gly,, 12869,C,T,synonymous_variant,nad5,p.His356His,, 12881,A,C,synonymous_variant,nad5,p.Gly360Gly,, 12960,G,A,missense_variant,nad5,p.Ala387Thr,, 12973,C,T,missense_variant,nad5,p.Thr391Ile,, 13070,T,C,synonymous_variant,nad5,p.Ala423Ala,, 13170,G,A,missense_variant,nad5,p.Ala457Thr,, 13256,C,A,synonymous_variant,nad5,p.Thr485Thr,, 13302,G,A,missense_variant,nad5,p.Val501Ile,, 13529,C,T,synonymous_variant,nad5,p.Asp576Asp,, 13664,T,C,missense_variant,cob_0,p.Phe5Ser,, 13674,C,T,synonymous_variant,cob_0,p.Ser8Ser,, 13719,C,T,synonymous_variant,cob_0,p.Pro23Pro,, 13968,T,C,synonymous_variant,cob_0,p.Gly106Gly,, 14001,C,T,synonymous_variant,cob_0,p.Gly117Gly,, 14052,G,A,synonymous_variant,cob_0,p.Leu134Leu,, 14244,T,C,synonymous_variant,cob_0,p.Leu198Leu,, 14298,C,T,synonymous_variant,cob_0,p.Cys216Cys,, 14346,G,A,synonymous_variant,cob_0,p.Gly232Gly,, 14354,T,C,missense_variant,cob_0,p.Ile235Thr,, 14385,A,G,synonymous_variant,cob_0,p.Leu245Leu,, 14440,G,A,missense_variant,cob_0,p.Val264Ile,, 14552,T,C,missense_variant,cob_0,p.Val301Ala,, 14571,G,A,synonymous_variant,cob_0,p.Leu307Leu,, 14593,G,A,missense_variant,cob_0,p.Ala315Thr,, 14609,C,T,missense_variant,cob_0,p.Pro320Leu,, 14641,G,A,missense_variant,cob_0,p.Ala331Thr,, 14751,C,T,synonymous_variant,cob_0,p.Phe367Phe,, 14790,C,T,synonymous_variant,cob_0,p.Tyr380Tyr,, 14810,T,C,non_coding_transcript_variant,trnT,,, 14866,T,C,intergenic_region,trnT-trnP,,, 14969,G,A,synonymous_variant,nad6,p.Ala169Ala,, 14989,G,A,missense_variant,nad6,p.Arg163Cys,, 15038,T,C,synonymous_variant,nad6,p.Val146Val,, 15039,A,C,missense_variant,nad6,p.Val146Gly,, 15041,T,C,synonymous_variant,nad6,p.Leu145Leu,, 15164,G,A,synonymous_variant,nad6,p.Val104Val,, 15166,C,T,missense_variant,nad6,p.Val104Ile,, 15172,A,G,synonymous_variant,nad6,p.Leu102Leu,, 15176,C,T,synonymous_variant,nad6,p.Met100Met,, 15200,C,T,synonymous_variant,nad6,p.Val92Val,, 15203,C,T,synonymous_variant,nad6,p.Gly91Gly,, 15251,C,T,synonymous_variant,nad6,p.Ala75Ala,, 15293,T,C,synonymous_variant,nad6,p.Gly61Gly,, 15323,T,C,synonymous_variant,nad6,p.Val51Val,, 15332,G,A,synonymous_variant,nad6,p.Val48Val,, 15435,A,C,missense_variant,nad6,p.Leu14Trp,, 15713,C,T,intergenic_region,Control Region,,, 15728,A,G,intergenic_region,Control Region,,, 15797,C,T,intergenic_region,Control Region,,, 15858,T,C,intergenic_region,Control Region,,, 16034,G,A,intergenic_region,Control Region,,, 16366,A,G,intergenic_region,Control Region,,,