LOCUS Exported 7091 bp ds-DNA circular SYN 21-SEP-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 7091) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Sep 21, 2020 from SnapGene 5.1.6 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7091 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 1..130 /label=AAV2 ITR (alternative) enhancer 223..526 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 528..805 /label=chicken beta-actin promoter intron 807..1824 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" protein_bind 1928..1961 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 1972..2005 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." CDS complement(2035..3747) /codon_start=1 /gene="Slc40a1" /gene_synonym="Dusg; Fpn1; IREG1; MTP; MTP1; Ol5; Pcm;Slc11a3; Slc39a1" /product="solute carrier family 40 member 1" /label=Slc40a1 C326S /note="ferroportin 1; metal transporter protein 1; iron-regulated transporter 1; solute carrier family 39 (iron-regulated transporter), member 1; duodenal-specific; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; metal transporting protein 1; polycythaemia; SLC11A3 iron transporter" /db_xref="CCDS:CCDS14933.1" /db_xref="GeneID:53945" /db_xref="MGI:MGI:1315204" /protein_id="NP_058613.2" /translation="MTKARDQTHQEGCCGSLANYLTSAKFLLYLGHSLSTWGDRMWHFA VSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVI LCGIILMMVFLHKNELLTMYHGWVLTVCYILIITIANIANLASTATAITIQRDWIVVVA GENRSRLADMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYFL LWKVYQKTPALAVKAALKVEESELKQLTSPKDTEPKPLEGTHLMGEKDSNIRELECEQE PTCASQMAEPFRTFRDGWVSYYNQPVFLAGMGLAFLYMTVLGFDSITTGYAYTQGLSGS ILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLFSGLAQLSCLILCVISVFMPGSPLD LSVSPFEDIRSRFVNVEPVSPTTKIPETVFTTEMHMSNMSNVHEMSTKPIPIVSVSLLF AGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLDLLHFIMVILAPNPE AFGLLVLISVSFVAMGHLMYFRFAQKTLGNQIFVCGPDEKEVTDENQPNTSVV" protein_bind complement(3764..3797) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(3808..3841) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." misc_feature 3861..4105 /label=WPRE3 /note="truncated WPRE element" polyA_signal 4124..4331 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 4354..4494 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 4569..5024 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5306..5410 /gene="bla" /label=AmpR promoter CDS 5411..6271 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6442..7030 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgaaacaat tctgcaggaa tctagttatt aatagtaatc 181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc 541 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 601 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg 661 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa 721 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta 781 taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg ccccgtgccc 841 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag 901 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg 961 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc 1021 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc 1081 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc 1141 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag 1201 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg 1261 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct 1321 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg 1381 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag 1441 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc 1501 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag 1561 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc 1621 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt 1681 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc 1741 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt 1801 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat 1861 cattttggca aagaattgat ttgataccgc gggccccgcg tggacggtac ccttcaggtc 1921 gacgtcgata acttcgtata gcatacatta tacgaagtta tccgaatcgc aataacttcg 1981 tataggatac tttatacgaa gttatttggc gcgccaaatt tgggccctct agacttatac 2041 aacagatgta ttcggttgat tttcatctgt aacttctttt tcatcaggac cacaaacaaa 2101 aatctggttg cccagagtct tctgggcaaa tcggaaatac ataagatgtc ccattgccac 2161 aaaggagact gaaatcaata ccagcaagcc aaaagcttca ggatttgggg ccaagatgac 2221 catgatgaaa tgcagaaggt caagaaggta gttcatggag ttctgcacac cattgataat 2281 gcctctttca gattcaatta cattttcttg cagcaactgt gtcaccgtca aatcaaagga 2341 ccaaagaccg attctagcag caatgactcc tgcaaacagc aggctgacag agactatggg 2401 gatgggttta gtactcatct catggacatt agacatgttg gacatatgca tttctgttgt 2461 aaagacggtc tcaggtattt tggtagttgg ggacactggc tccacattca caaacctaga 2521 acggatatct tcaaatggag aaacagacag gtccaagggg cttccaggca tgaatacgga 2581 gatcacacac aagattaaac aggaaagctg ggctagtcct gagaatagac cagtccgaac 2641 aaggccacat tttcgacgta gccaggtgaa ggccacagtt cccattattc cagttattgc 2701 tgatgctccc atcaaaatac taaggatgga tccactcagc ccctgagtgt aggcgtaccc 2761 tgtagtgatg gagtcaaagc ccaggactgt catatagagg aaagccaggc ccatgccagc 2821 cagaaacact ggctggttat agtaggagac ccatccatct cggaaagtgc ggaagggctc 2881 tgccatctgg gaggcacagg tgggctcttg ttcacattca agttcacgga tgttggagtc 2941 tttctcaccc attagatgag ttccctccaa aggttttggc tcagtatctt taggtgaggt 3001 cagctgcttc agttctgact cctctacctt gagagcagct tttacagcca gagcaggggt 3061 cttctggtaa accttccaga gcaagaagta ctccacacac atggacacca aattccaacc 3121 ggaaataaaa ccacagccaa tgactggaga accaaatgtc ataatctggc cgacagccat 3181 gggggccagg atgttggtta gctggtcaat ccttctaatg gtagcattca tgtctgctaa 3241 tctgctcctg ttttctcctg ccacaacaac aatccagtcc ctttggattg tgatcgcagt 3301 ggcagtactg gccaaatttg caatgtttgc aatagtgatg atcaggatgt agcagacagt 3361 aaggacccat ccatggtaca tggtcaggag ctcattcttg tgtaggaaaa ccatcatcag 3421 gatgattccg cagaggatga cggacacatt ctgaaccacc agtgacgtct gggccacttt 3481 aagtctggca ttcttatcca cccagtcacc aatgatggct cccaggacca gaacagagcc 3541 tgccaccacc agtccataga cagctgtcaa gagaaggctg tttccataga gttccaccag 3601 aaacacagac actgcaaagt gccacatccg atccccccaa gtggagagag agtggccaag 3661 gtagaggagg aattttgctg aggtcaggta gtttgctaag gatccacagc atccttcctg 3721 atgggtttga tctcttgcct tggtcatggt ggcgccggcc ttgataactt cgtataatgt 3781 atgctatacg aagttatccg aatcgcaata acttcgtata aagtatccta tacgaagtta 3841 tcgaagatca tcaagcaaac aatcaacctc tggattacaa aatttgtgaa agattgactg 3901 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 3961 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag 4021 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4081 tgttgggcac tgacaattcc gtggtgagat cgatctgcct cgactgtgcc ttctagttgc 4141 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 4201 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 4261 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 4321 catgctgggg acacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 4381 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 4441 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 4501 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca 4561 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4621 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt 4681 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 4741 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg 4801 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 4861 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt 4921 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca 4981 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag 5041 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 5101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 5161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 5221 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 5281 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 5341 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 5401 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 5461 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 5521 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 5581 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 5641 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 5701 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 5761 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 5821 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 5881 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 5941 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 6001 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 6061 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 6121 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 6181 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 6241 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 6301 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 6361 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 6421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 6481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 6541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 6601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 6661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 6721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 6781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 6841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 6901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 6961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 7021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 7081 tgctcacatg t //