FungiTremellalesTremellaceaeFanLong-FeiAlvarengaRenato Lúcio MendesGibertoniTatiana BaptistaWuFangDaiYu-ChengFour new species in the Tremellafibulifera complex (Tremellales, Basidiomycota)MycoKeys048202182335610.3897/mycokeys.82.63241 058D9F07-1D89-5218-994C-048A93A16083 T. neofibulifera Kobayasi, Scientific Report, Tokyo Bunrika Daigaku, Section 4: 15 (1939)Figs 3D, 8Basidioma.

Sessile, when fresh soft gelatinous, creamy-white to pale yellowish, irregularly cerebriform or slightly foliose, with undulate lobes, up to 4.5 cm long, 2.0 cm broad and 2.5 cm high from base, becoming firmly gelatinous and invisible yellowish when dry, broadly attached to substratum.

Internal features.

Hyphae hyaline, smooth thin- to slightly thick-walled, 2.0–6.0 µm in diameter, branched, interwoven, with abundant clamp connections, clamp complexes and anastomoses, slightly thick-walled hyphae usually present near to base of basidioma, sometimes swollen up to 8.5 μm; hyphidia hyaline, smooth, thin-walled, arranged in cluster, usually parallel; vesicles infrequent, thick-walled; swollen cells and haustoria absent; mature basidia thin-walled, ovoid to subglobose, with a basal clamp connection, 14.0–16.0 × 13.0–17.0 μm, L = 14.9 µm, W = 14.8 µm, Q = 1.01 (n = 30/1), sometimes their width greater than length, usually longitudinally septate, rarely obliquely septate, 2–4-celled, with obvious oil drops; sterigmata up to 70 μm long, 1.5–2.0 in diameter, slightly protuberant at apex; probasidia thin-walled, ellipsoid to subglobose, usually proliferating from terminal hyphae; basidiospores hyaline, thin-walled, ellipsoid to broadly ellipsoid, apiculate, with oil drops, 8.0–10.0 × 6.0–8.0 μm, L = 8.9 µm, W = 6.5 µm, Q = 1.37 (n = 30/1), germination by germ tubes or secondary spores; conidia absent.

10.3897/mycokeys.82.63241.figure8B5B480F3-496B-5DC7-A387-4E849B1D3676

Microscopic structures of Tremellaneofibulifera” (Wu 248) A basidiospores B germination tubes of basidiospores and secondary spores C–E basidia at different stages F probasidia G hyphae with clamp connections and clamp complexes H vesicles I parallel hyphidia in hymenium. Scale bars: 10 μm (A–I).

https://binary.pensoft.net/fig/574254
Specimens examined.

China Jilin, Helong, Quanshuidong Forest Farm, on stump of Quercus, 15 July 2017, F. Wu 243 (BJFC031046); F. Wu 244 (BJFC031047); F. Wu 248 (BJFC031051).

Notes.

Three specimens listed above from Northeast China together with LE303445 from Far East of Russia formed a distinct lineage closely related to T.guangxiensis in our phylogenies (Figs 1, 2). T.neofibulifera was originally described from Japan (Kobayasi 1939), and our studied East Asian samples have similar morphology to T.neofibulifera except bigger basidiospores (Table 3). We fail to loan the type of T.neofibulifera, and for the time being we treat our studied East Asia samples as T.neofibulifera”. The current T.neofibulifera” differs from other similar species of the Tremellafibulifera complex by the parallel hyphidia and the presence of vesicles. In addition, T.neofibulifera” are different from T.guangxiensis, T.australe, T.subfibulifera and T.fibulifera s.s. by 3.15%, 5.25%, 7.14%, and 8.19% sequence differences in the ITS sequences and 2.04%, 1.32%, 3.18%, and 2.41% in the partial nLSU sequences respectively.

10.3897/mycokeys.82.63241.figure3E0A1FEB9-2EF3-5796-BCB1-757C2D7BC6F4

Basidioma ATremellafibulifera (Alvarenga 471) BT.australe (Wu 154) CT.guangxiensis (Wu 3) DT.latispora (Dai 17568) ET.neofibulifera” (Wu 244) FT.subfibulifera (Alvarenga 334). Scale bars: 1 cm (A–F).

https://binary.pensoft.net/fig/574249
10.3897/mycokeys.82.63241.figure156EF2716-1257-5CC8-A0A6-2DFE49EF4B5F

The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s.s. based on the ITS + partial nLSU dataset. Bootstrap support values for MP and ML greater than 50% and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

https://binary.pensoft.net/fig/574247
10.3897/mycokeys.82.63241.figure2FAB98D23-54E8-52B6-A6C5-1FD981449F5A

The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s.s. based on the combined ITS + partial nLSU + mtSSU + TEF1 + RPB1 + RPB2 dataset. Bootstrap support values for MP and ML greater than 50% and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

https://binary.pensoft.net/fig/574248

A morphological comparison of taxa in the Tremellafibulifera complex.

TaxaBasidia (µm)Basidiospores (µm)Conidia (µm)HyphidiaDistributionReference
T. fibulifera 12.0–16.07.0–10.03.5UnknownBrazil Möller 1895
T. fibulifera 15.0–18.0 × 9.0–13.08.0–9.0 × 5.0–8.0Not observedUnknownBrazil Bandoni and Oberwinkler 1983
T.fibulifera s.s.13.0–18.0 × 9.0–16.07.0–10.0 × 6.0–7.02.0–3.0 × 1.0–2.5BranchedBrazilPresent study
T. australe 14.0–19.0 × 13.0–17.08.0–10.0 × 6–8.0AbsentPresentChinaPresent study
T. cheejenii 12.0–17.0 × 13.0–18.05.0–10.0 × 4.5–8.02.2–4.0 × 1.8–3.0BranchedChina Zhao et al. 2019
T. guangxiensis 14.0–17.0 × 14.0–16.08.0–9.5 × 6.0–7.52.0–3.2 × 1.8–3.0BranchedChinaPresent study
T. latispora 17.2–24.0 × 17.0–23.010.1–11.8 × 9.9–11.42.8–3.6 × 1.8–3.0PresentChinaPresent study
T. lloydiae-candidae 14.0–20.0 × 13.0–16.07.5–10AbsentUnknownJapan, Russia Malysheva et al. 2015
T. olens Unknown12.7–14.5AbsentUnknownAustralia Hooker 1860
T. neofibulifera 13.2–15.5 × 9–105.5–8.5 × 4.5–5.5AbsentUnknownJapan Kobayasi 1939
T.neofibulifera14.0–16.0 × 13.0–17.08.0–10.0 × 6.0–8.0AbsentParallelChina, RussiaPresent study
T. subfibulifera 14.4–20.3 × 12.8–16.35.4–9.8 × 4.2–6.02.0–3.0 × 0.5–1.0AbsentBrazilPresent study
KobayasiY (1939) On the genus Tremella and its allies from Japan.Science Report of the Tokyo Bunrika Diagaku4: 126.MöllerA (1895) Protobasidiomyceten.Botanische Mittheilungen aus den Tropen8: 1180.BandoniRJOberwinklerF (1983) On some species of Tremella described by Alfred Möller.Mycologia75: 854863. https://doi.org/10.1080/00275514.1983.12023760ZhaoYLiuXZBaiFY (2019) Four new species of Tremella (Tremellales, Basidiomycota) based on morphology and DNA sequence data.Mycokeys47: 7595. https://doi.org/10.3897/mycokeys.47.29180MalyshevaVFMalyshevaEFBulakhEM (2015) The genus Tremella (Tremellales, Basidiomycota) in Russia with description of two new species and proposal of one nomenclatural combination.Phytotaxa238: 4070. https://doi.org/10.11646/phytotaxa.238.1.2HookerJD (1860) Botany of the Antarctic Voyage.III Flora Tasmaniae2: 1422.