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Extended Data Figure 3b (right panel): Kruskal–Wallis test with Wilcoxon rank sum test (quantification of dsRed immunofluorescence with draper-RNAi)
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A: Sham Vehicle  
B: Severe dTBI Vehicle
C: Severe dTBI RU

  group1 group2    padjusted 
      B      A 1.346076e-06 
      C      A 2.393024e-06  
      C      B 4.744838e-02   


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Extended Data Figure 3c: Kruskal–Wallis test with Wilcoxon rank sum test and Holm adjustment (RT-qPCR for dsRed and draper at 1 dpi with draper-RNAi)
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A: Sham vehicle  
B: Sham RU
C: Severe dTBI RU
D: Severe dTBI Vehicle

   group1 group2  padjusted target.gene 
       B      A 0.01298701       dsRed 
       C      A 0.01298701       dsRed
       D      A 0.01298701       dsRed 
       C      B 0.01298701       dsRed 
       D      B 0.01298701       dsRed 
       C      B 0.01298701      draper   
       D      C 0.01298701      draper 


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Extended Data Figure 3e (right panel): Kruskal–Wallis test with Dunn’s multiple comparison test and Holm adjustment (quantification of lysotracker immunofluorescence in draper null flies)
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A: Sham, draper-null   
B: Severe dTBI, draper-null
C: Severe dTBI, WT (w1118)

  group1 group2  padjusted 
      B      A 0.00061703
      C      A 0.00061703
      C      B 0.48125095


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Extended Data Figure 3g: Kruskal–Wallis test with Wilcoxon rank sum test (quantification of dsRed immunofluorescence with basket-RNAi)
**********************************************************************
A: Sham Vehicle  
B: Severe dTBI Vehicle
C: Severe dTBI RU

  group1 group2    padjusted Condition y_pos stars
1      B      A 0.0002598021      Sham   5.5   ***
2      C      A 0.0009872480      Sham   5.0   ***
3      C      B 0.1127541189      Sham     4      


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Extended Data Figure 3h: Welch Two Sample t-test (RT-qPCR for dsRed and basket)
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dsRed mRNA
*****************************************************
  Welch Two Sample t-test

data:  Expression_fold_change by Treatment
t = 4.0426, df = 5.5322, p-value = 0.00803
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 0.2303991 0.9755733
sample estimates:
mean in group veh  mean in group RU 
         1.033077          0.430091 

*****************************************************
basket (JNK) mRNA
*****************************************************
  Welch Two Sample t-test

data:  Expression_fold_change by Treatment
t = 5.6945, df = 4.2595, p-value = 0.003876
alternative hypothesis: true difference in means is not equal to 0
95 percent confidence interval:
 0.4839611 1.3634691
sample estimates:
mean in group veh  mean in group RU 
        1.0400855         0.1163704