Fungi Distoseptisporales Distoseptisporaceae ShenHong-WeiBaoDan-FengHydeKevin D.SuHong-YanBhatDarbhe J.LuoZong-LongTwo novel species and two new records of Distoseptispora from freshwater habitats in China and ThailandMycoKeys08112021847910110.3897/mycokeys.84.71905 68BD6629-9E92-5AC8-BE36-175569F80A7A Distoseptispora thysanolaenae Index Fungorum Number No: IF555408 Facesoffungi Number No: FoF05011 Goonas., Dayarathne, Phookamsak & K.D.Hyde, in Phookamsak et al., Fungal Diversity 95: 126 (2019)Figure 5Description.

Saprobic on submerged wood in freshwater River. Sexual morph: Undetermined. Asexual morph: Colonies on the substratum superficial, effuse, scattered, hairy, dark brown. Mycelium partly immersed, composed of branched, septate, smooth, brown to dark brown hyphae. Conidiophores 41–59 × 4–5 μm (x¯ = 50 × 5 μm, n = 20) macronematous, mononematous, unbranched, single, erect, straight or slightly curved, smooth, 3–6-septate, pale brown to brown. Conidiogenous cells monoblastic, integrated, determinate, terminal, dark brown, cylindrical. Conidia 46–87 × 9–12 μm (x¯ = 67 × 10 μm, n = 25) acrogenous, solitary, dry, smooth, obclavate, elongated, straight or slightly curved, truncate at base, tapering towards apex, 6–19-septate, dark grayish-brown to light yellow-green, thick-walled.

10.3897/mycokeys.84.71905.figure5A8D41106-C22F-5701-9EC6-72543B0BD308

Distoseptisporathysanolaenae (KUN-HKAS 112710) A colonies on the substratum B-D conidiophores with conidia E, F conidiogenous cells G-N conidia O germinating conidium P, Q culture on PDA Scale bars: 30 μm (B-D); 10 μm (E, F); 20 μm (G-O).

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Culture characteristics.

Conidia cultivated on PDA within 12 h and germ tubes produced at the apex. Colonies on PDA, reaching 6 cm after 6 weeks at room temperature (25 ℃). Mycelium loose, flocculent, neat edges, convex in middle, pale brown to dark brown. Black, smooth on the back.

Material examined.

China, Yunnan Province, Lushui City, Nujiang River, 26°23'12"N; 98°53'94"E, on submerged decaying wood, 3 May 2016, Zonglong Luo and Songming Tang, S-876 (KUN-HKAS 112710), living culture (DLUCC 876 = KUNCC 21-10710)

Notes.

Our new collection is identical to Distoseptisporathysanolaenae in characters of the conidiophores, conidiogenous cell, and conidia (Phookamsak et al. 2019). Furthermore, our new isolate phylogenetically clusters with the ex-type strain of D.thysanolaenae (KUN-HKAS 102247) with 100%ML/1.00PP support (Figure 1). Distoseptisporathysanolaenae was collected from terrestrial habitats in China, while, our new isolate was collected from freshwater habitat in China. Therefore, we identified our new collection as D.thysanolaenae, and it is new to freshwater habitats in China.

10.3897/mycokeys.84.71905.figure1C2858D9A-6009-52A1-A07E-AFA5CE3900A2

Maximum likelihood (ML) tree is based on combined of LSU, ITS, SSU, TEF1-α, and RPB2 sequence data. Bootstrap support values with an ML greater than 70% and Bayesian posterior probabilities (PP) greater than 0.97 given above the nodes, shown as “ML/PP”. The tree is rooted with Pseudostanjehughesiaaquitropica (MFLUCC 16-0569) and P.lignicola (MFLUCC 15-0352). New species are indicated in red and type strains are in bold.

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PhookamsakRHydeKDJeewonRBhatDJJonesEBGMaharachchikumburaSSNRaspéOKarunarathnaSCWanasingheDNHongsananSDoilomMTennakoonDSMachadoARFirminoALGhoshAKarunarathnaAMešićADuttaAKThongbaiBDevadathaBNorphanphounCSenwannaCWeiDPemDAckahFKWangGNJiangHBMadridHLeeHBGoonasekaraIDManawasingheISKušanCano JGenéJLiJDasKAcharyaKRajKNALathaKPDChethanaKWTHeMQDueñasMJadanMMartínMPSamarakoonMCDayarathneMCRazaMParkMSTelleriaMTChaiwanNMatočecNde SilvaNIPereiraOLSinghPNManimohanPUniyalPShangQJBhattRPPereraRHAlvarengaRLMNogal-PrataSSinghSKVadthanaratSOhSYHuangSKRanaSKontaSPaloiSJayasiriSCJeonSJMehmoodTGibertoniTBNguyenTTTSinghUThiyagarajaVSarmaVVDongWYuXDLuYZLimYWChenYTkalčecZZhangZFLuoZLDaranagamaDAThambugalaKMTibprommaSCamporesiEBulgakovTDissanayakeAJSenanayakeICDaiDQTangLZKhanSZhangHPromputthaICaiLChomnuntiPZhaoRLLumyongSBoonmeeSWenTCMortimerPEXuJ (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungal taxa.Fungal Diversity95: 1273. https://doi.org/10.1007/s13225-019-00421-w