Fungi Lecanorales Parmeliaceae SzczepańskaKatarzynaGuzow-KrzemińskaBeataUrbaniakJacekInfraspecific variation of some brown Parmeliae (in Poland) – a comparison of ITS rDNA and non-molecular charactersMycoKeys221220218512716010.3897/mycokeys.85.70552 Melanelia agnata (Nyl.) A. Thell Nova Hedwigia 60:416 (1995) ≡ Platysmaagnatum Nyl., Flora, Jena 60:562 (1877) ≡ Cetrariaagnata (Nyl.) Kristinsson, Lichenologist 6:144 (1974). Description.

M.agnata has foliose thallus with flat, smooth, 0.25–2 mm broad lobes which are thicker on the margins and rounded at the ends (Szczepańska and Kossowska 2017). The upper surface is glossy, olive-brown to dark brown. The lower surface is pale brown to dark brown in the centre, with single, dark rhizines. M.agnata possess pseudocyphellae which are larger on the lobe margins and smaller, punctiform on the upper surface of the lobes. Pycnidia are mainly marginal to laminal, partially immersed and globose with hyaline bacilliform conidia (4.5–5.5 × 1 µm). Apothecia are not seen in examined material.

Chemistry.

No secondary metabolites were detected by TLC.

Distribution.

M.agnata is a rare taxon occurring in arctic and boreal regions in North America and Europe, growing in open stands on siliceous and basalt rocks (Otte et al. 2005). Available molecular data concern samples collected only in North America (Greenland) and North Europe (Iceland, Norway).

Haplotypes differentiation.

Six different haplotypes were identified in M.agnata (n = 10), of which two Polish specimens, collected in the Karpaty Mountains, have the same, not previously known, haplotype (Fig. 3, Table 2). It differs from other haplotypes in at least seven positions. However, the remaining specimens originate from Greenland, Iceland or Norway and no other samples from Central Europe have been sequenced until now. Four Icelandic specimens have the same haplotype, which is similar to the haplotype from Norwegian specimens. In contrast, Icelandic haplotypes differ from Greenlandic haplotypes in at least eight positions. Whether their genetic diversity supports conclusions from previous papers suggesting potentially unrecognised species lineages in the M.agnata genus (Leavitt et al. 2014; Xu et al. 2017) remains unresolved and should be further studied.

10.3897/mycokeys.85.70552.figure3

Haplotype network, based on ITS rDNA sequences from specimens of Melaneliaagnata. Newly-generated sequences are described with isolate numbers preceding the species names. Sequences downloaded from GenBank are described with their accession numbers. Mutational changes are presented as numbers in brackets near lines between haplotypes.

https://binary.pensoft.net/fig/626674

List of haplotypes identified in this study and their geographical distribution. Nucleotide diversity for each species is also presented, and the newly generated sequences are in bold.

Haplotypes numberNorth AmericaNorth EuropeCentral EuropeWest EuropeAsiaNucleotide diversity
Cetrariacommixta
1KF257934 GreenlandAF451796 Finland 37 Poland
KF257937 GreenlandKY266843 Norway 97 Poland 0.00405
KF257938 GreenlandKC990132 Sweden 129 Germany
GU994554 Sweden
2 36 Poland
124 Poland
3AF451797 Canada
4KF257939 Greenland
5KF257936 Greenland
6KF257935 Greenland
7GU994555 Spain
Melaneliaagnata
1KY508672 Iceland
KY508673 Iceland0.01552
KY963373 Iceland
KY963374 Iceland
2 102 Poland
103 Poland
3KF257940 Greenland
4KF257941 Greenland
5KF257942 Greenland
6MK257942 Norway
Melaneliahepatizon
1KF257943 GreenlandKY508678 Iceland 98 Poland
KF257944 GreenlandKY508680 Iceland 111 Poland 0.01421
KY508679 Norway 128 Germany
MK812188 Norway
2KF257945 GreenlandKY508675 Iceland 109 Poland
KF257946 GreenlandKY508676 Iceland
KF257947 Greenland
KF257949 Greenland
KF257951 Greenland
3KY508674 Iceland 95 Poland
KY508677 Iceland 110 Poland
KY266879 Iceland
KY266879 Norway
DQ980016 Sweden
4 83 Poland AF451776 Italy
96 Poland
113 Poland
116 Slovakia
119 Poland
122 Poland
5KF257950 Greenland
KF257953 Greenland
6KF257952 Greenland
7KF257948 Greenland
8AF141369 Canada
9KY963376 Iceland
10MK812512 Norway
11 91 Poland
12 117 Slovakia
Melaneliastygia
1AY611097 FinlandAY611121 AustriaAF451775 Italy
MK812608 Norway 40 Poland 0.01418
42 Austria
94 Poland
104 Poland
108 Poland
112 Poland
127 Czech Republic
2KY508681 Island
KY508682 Island
KY508683 Island
KY963375 Island
3KF257954 Greenland
KF257955 Greenland
4AF115763 Finland
5MK812312 Norway
Montaneliadisjuncta
1KF257964 CanadaKY963378 IcelandAY611077 AustriaGU994556 India
KF257967 CanadaKF257961 Norway 50 Poland KF257972 India0.00380
KF257969 CanadaKP771829 Norway 51 Poland KP771837 India
KP771830 CanadaKP771834 Sweden 57 Poland
JX126181 USA 80 Poland
81 Poland
82 Poland
85 Poland
86 Poland
87 Poland
88 Poland
93 Poland
121 Poland
125 Poland
126 Poland
130 Czech Republic
2KF257962 CanadaKY963377 Iceland 90 Poland
KF257965 CanadaKY266910 Norway 120 Poland
KF257966 CanadaDQ980015 Sweden
KP771832 Greenland
KF257958 Greenland
KF257970 Greenland
KP771826 Greenland
3KF257957 GreenlandKY508684 Iceland
KF257971 GreenlandKY508685 Iceland
KP771825 GreenlandKY508686 Iceland
KP771833 Greenland
4- 78 Poland
JX974654 United Kingdom 79 Poland
KP771835 United Kingdom 89 Poland
92 Poland
123 Poland
5KF257956 Canada
KP771824 Canada
6JX974658 Canada
KP771836 Canada
7KF257963 Canada
8KF257959 Greenland
KP771827 Greenland
9KF257968 USA
KP771831 USA
10KF257960 USA
KP771828 USA
11MK811852 Norway
12MK811711 Norway
Montaneliasorediata
1MK811977 Norway 100 Poland
MK811965 Norway0.00830
GU994557 Sweden
KP771845 Sweden
2KF257978 USAKF257981 Russia
KP771846 USAKP771847 Russia
KM386101 Japan
3KF257980 USA 101 Poland
4KM386102 Canada
KF257982 Canada
5KF257979 Canada
6MK811963 Norway
SzczepańskaKKossowskaM (2017) Cetrariellacommixta and the genus Melanelia (Parmeliaceae, Ascomycota) in Poland.Herzogia30: 272288. https://doi.org/10.13158/heia.30.1.2017.272OtteVEsslingerTLLitterskiB (2005) Global distribution of the European species of the lichen genus Melanelia Essl.Journal of Biogeography32: 12211241. https://doi.org/10.1111/j.1365-2699.2005.01268.xLeavittSDEsslingerTLHansenESDivakarPKCrespoALoomisBFLumbschHT (2014) DNA barcoding of brown Parmeliae (Parmeliaceae) species: a molecular approach for accurate specimen identification, emphasizing species in Greenland.Organisms Diversity & Evolution14: 1120. https://doi.org/10.1007/s13127-013-0147-1XuMHeidmarssonSThorsteinsdottirMEirikssonFFOmarsdottirSOlafsdottirES (2017) DNA barcoding and LC-MS metabolite profiling of the lichen-forming genus Melanelia: Specimen identification and discrimination focusing on Icelandic taxa. PLоS ONE 12(5): e0178012. https://doi.org/10.1371/journal.pone.0178012