Supplementary Table 2 Primers applied for RT-PCR amplification of SARS-CoV-2,,,,,
name,pool,seq,length,%gc,tm (use 65)
nCoV-2019_1_LEFT,nCoV-2019_1,ACCAACCAACTTTCGATCTCTTGT,24,41.67,60.69
nCoV-2019_1_RIGHT,nCoV-2019_1,CATCTTTAAGATGTTGACGTGCCTC,25,44,60.45
nCoV-2019_2_LEFT,nCoV-2019_2,CTGTTTTACAGGTTCGCGACGT,22,50,61.67
nCoV-2019_2_RIGHT,nCoV-2019_2,TAAGGATCAGTGCCAAGCTCGT,22,50,61.74
nCoV-2019_3_LEFT,nCoV-2019_1,CGGTAATAAAGGAGCTGGTGGC,22,54.55,61.32
nCoV-2019_3_RIGHT,nCoV-2019_1,AAGGTGTCTGCAATTCATAGCTCT,24,41.67,60.32
nCoV-2019_4_LEFT,nCoV-2019_2,GGTGTATACTGCTGCCGTGAAC,22,54.55,61.56
nCoV-2019_4_RIGHT,nCoV-2019_2,CACAAGTAGTGGCACCTTCTTTAGT,25,44,60.97
nCoV-2019_5_LEFT,nCoV-2019_1,TGGTGAAACTTCATGGCAGACG,22,50,61.39
nCoV-2019_5_RIGHT,nCoV-2019_1,ATTGATGTTGACTTTCTCTTTTTGGAGT,28,32.14,60.17
nCoV-2019_6_LEFT,nCoV-2019_2,GGTGTTGTTGGAGAAGGTTCCG,22,54.55,61.64
nCoV-2019_6_RIGHT,nCoV-2019_2,TAGCGGCCTTCTGTAAAACACG,22,50,61.18
nCoV-2019_7_LEFT,nCoV-2019_1,ATCAGAGGCTGCTCGTGTTGTA,22,50,61.73
nCoV-2019_7_RIGHT,nCoV-2019_1,TGCACAGGTGACAATTTGTCCA,22,45.45,60.95
nCoV-2019_8_LEFT,nCoV-2019_2,AGAGTTTCTTAGAGACGGTTGGGA,24,45.83,61
nCoV-2019_8_RIGHT,nCoV-2019_2,GCTTCAACAGCTTCACTAGTAGGT,24,45.83,60.56
nCoV-2019_9_LEFT,nCoV-2019_1,TCCCACAGAAGTGTTAACAGAGGA,24,45.83,61.18
nCoV-2019_9_RIGHT,nCoV-2019_1,ATGACAGCATCTGCCACAACAC,22,50,61.71
nCoV-2019_10_LEFT,nCoV-2019_2,TGAGAAGTGCTCTGCCTATACAGT,24,45.83,61.12
nCoV-2019_10_RIGHT,nCoV-2019_2,TCATCTAACCAATCTTCTTCTTGCTCT,27,37.04,60.31
nCoV-2019_11_LEFT,nCoV-2019_1,GGAATTTGGTGCCACTTCTGCT,22,50,61.66
nCoV-2019_11_RIGHT,nCoV-2019_1,TCATCAGATTCAACTTGCATGGCA,24,41.67,61.35
nCoV-2019_12_LEFT,nCoV-2019_2,AAACATGGAGGAGGTGTTGCAG,22,50,61.08
nCoV-2019_12_RIGHT,nCoV-2019_2,TTCACTCTTCATTTCCAAAAAGCTTGA,27,33.33,60.36
nCoV-2019_13_LEFT,nCoV-2019_1,TCGCACAAATGTCTACTTAGCTGT,24,41.67,60.56
nCoV-2019_13_RIGHT,nCoV-2019_1,ACCACAGCAGTTAAAACACCCT,22,45.45,60.36
nCoV-2019_14_LEFT,nCoV-2019_2,CATCCAGATTCTGCCACTCTTGT,23,47.83,60.62
nCoV-2019_14_RIGHT,nCoV-2019_2,AGTTTCCACACAGACAGGCATT,22,45.45,60.42
nCoV-2019_15_LEFT,nCoV-2019_1,ACAGTGCTTAAAAAGTGTAAAAGTGCC,27,37.04,61.32
nCoV-2019_15_RIGHT,nCoV-2019_1,AACAGAAACTGTAGCTGGCACT,22,45.45,60.16
nCoV-2019_16_LEFT,nCoV-2019_2,AATTTGGAAGAAGCTGCTCGGT,22,45.45,60.82
nCoV-2019_16_RIGHT,nCoV-2019_2,CACAACTTGCGTGTGGAGGTTA,22,50,61.32
nCoV-2019_17_LEFT,nCoV-2019_1,CTTCTTTCTTTGAGAGAAGTGAGGACT,27,40.74,60.69
nCoV-2019_17_RIGHT,nCoV-2019_1,TTTGTTGGAGTGTTAACAATGCAGT,25,36,60.11
nCoV-2019_18_LEFT,nCoV-2019_2,TGGAAATACCCACAAGTTAATGGTTTAAC,29,34.48,60.69
nCoV-2019_18_RIGHT,nCoV-2019_2,AGCTTGTTTACCACACGTACAAGG,24,45.83,61.51
nCoV-2019_19_LEFT,nCoV-2019_1,GCTGTTATGTACATGGGCACACT,23,47.83,61.18
nCoV-2019_19_RIGHT,nCoV-2019_1,TGTCCAACTTAGGGTCAATTTCTGT,25,40,60.4
nCoV-2019_20_LEFT,nCoV-2019_2,ACAAAGAAAACAGTTACACAACAACCA,27,33.33,60.68
nCoV-2019_20_RIGHT,nCoV-2019_2,ACGTGGCTTTATTAGTTGCATTGTT,25,36,60.28
nCoV-2019_21_LEFT,nCoV-2019_1,TGGCTATTGATTATAAACACTACACACCC,29,37.93,61.49
nCoV-2019_21_RIGHT,nCoV-2019_1,TAGATCTGTGTGGCCAACCTCT,22,50,60.83
nCoV-2019_22_LEFT,nCoV-2019_2,ACTACCGAAGTTGTAGGAGACATTATACT,29,37.93,61.25
nCoV-2019_22_RIGHT,nCoV-2019_2,ACAGTATTCTTTGCTATAGTAGTCGGC,27,40.74,60.73
nCoV-2019_23_LEFT,nCoV-2019_1,ACAACTACTAACATAGTTACACGGTGT,27,37.04,60.26
nCoV-2019_23_RIGHT,nCoV-2019_1,ACCAGTACAGTAGGTTGCAATAGTG,25,44,60.57
nCoV-2019_24_LEFT,nCoV-2019_2,AGGCATGCCTTCTTACTGTACTG,23,47.83,60.37
nCoV-2019_24_RIGHT,nCoV-2019_2,ACATTCTAACCATAGCTGAAATCGGG,26,42.31,61.19
nCoV-2019_25_LEFT,nCoV-2019_1,GCAATTGTTTTTCAGCTATTTTGCAGT,27,33.33,60.73
nCoV-2019_25_RIGHT,nCoV-2019_1,ACTGTAGTGACAAGTCTCTCGCA,23,47.83,61.3
nCoV-2019_26_LEFT,nCoV-2019_2,TTGTGATACATTCTGTGCTGGTAGT,25,40,60.28
nCoV-2019_26_RIGHT,nCoV-2019_2,TCCGCACTATCACCAACATCAG,22,50,60.42
nCoV-2019_27_LEFT,nCoV-2019_1,ACTACAGTCAGCTTATGTGTCAACC,25,44,60.8
nCoV-2019_27_RIGHT,nCoV-2019_1,AATACAAGCACCAAGGTCACGG,22,50,61.13
nCoV-2019_28_LEFT,nCoV-2019_2,ACATAGAAGTTACTGGCGATAGTTGT,26,38.46,60.13
nCoV-2019_28_RIGHT,nCoV-2019_2,TGTTTAGACATGACATGAACAGGTGT,26,38.46,60.91
nCoV-2019_29_LEFT,nCoV-2019_1,ACTTGTGTTCCTTTTTGTTGCTGC,24,41.67,61.39
nCoV-2019_29_RIGHT,nCoV-2019_1,AGTGTACTCTATAAGTTTTGATGGTGTGT,29,34.48,60.69
nCoV-2019_30_LEFT,nCoV-2019_2,GCACAACTAATGGTGACTTTTTGCA,25,40,61.19
nCoV-2019_30_RIGHT,nCoV-2019_2,ACCACTAGTAGATACACAAACACCAG,26,42.31,60.3
nCoV-2019_31_LEFT,nCoV-2019_1,TTCTGAGTACTGTAGGCACGGC,22,54.55,62.03
nCoV-2019_31_RIGHT,nCoV-2019_1,ACAGAATAAACACCAGGTAAGAATGAGT,28,35.71,60.69
nCoV-2019_32_LEFT,nCoV-2019_2,TGGTGAATACAGTCATGTAGTTGCC,25,44,61.09
nCoV-2019_32_RIGHT,nCoV-2019_2,AGCACATCACTACGCAACTTTAGA,24,41.67,60.56
nCoV-2019_33_LEFT,nCoV-2019_1,ACTTTTGAAGAAGCTGCGCTGT,22,45.45,61.58
nCoV-2019_33_RIGHT,nCoV-2019_1,TGGACAGTAAACTACGTCATCAAGC,25,44,61.08
nCoV-2019_34_LEFT,nCoV-2019_2,TCCCATCTGGTAAAGTTGAGGGT,23,47.83,61.02
nCoV-2019_34_RIGHT,nCoV-2019_2,AGTGAAATTGGGCCTCATAGCA,22,45.45,60.03
nCoV-2019_35_LEFT,nCoV-2019_1,TGTTCGCATTCAACCAGGACAG,22,50,61.39
nCoV-2019_35_RIGHT,nCoV-2019_1,ACTTCATAGCCACAAGGTTAAAGTCA,26,38.46,60.69
nCoV-2019_36_LEFT,nCoV-2019_2,TTAGCTTGGTTGTACGCTGCTG,22,50,61.44
nCoV-2019_36_RIGHT,nCoV-2019_2,GAACAAAGACCATTGAGTACTCTGGA,26,42.31,60.74
nCoV-2019_37_LEFT,nCoV-2019_1,ACACACCACTGGTTGTTACTCAC,23,47.83,60.93
nCoV-2019_37_RIGHT,nCoV-2019_1,GTCCACACTCTCCTAGCACCAT,22,54.55,61.48
nCoV-2019_38_LEFT,nCoV-2019_2,ACTGTGTTATGTATGCATCAGCTGT,25,40,60.86
nCoV-2019_38_RIGHT,nCoV-2019_2,CACCAAGAGTCAGTCTAAAGTAGCG,25,48,61.13
nCoV-2019_39_LEFT,nCoV-2019_1,AGTATTGCCCTATTTTCTTCATAACTGGT,29,34.48,61
nCoV-2019_39_RIGHT,nCoV-2019_1,TGTAACTGGACACATTGAGCCC,22,50,60.55
nCoV-2019_40_LEFT,nCoV-2019_2,TGCACATCAGTAGTCTTACTCTCAGT,26,42.31,61.25
nCoV-2019_40_RIGHT,nCoV-2019_2,CATGGCTGCATCACGGTCAAAT,22,50,62.09
nCoV-2019_41_LEFT,nCoV-2019_1,GTTCCCTTCCATCATATGCAGCT,23,47.83,60.75
nCoV-2019_41_RIGHT,nCoV-2019_1,TGGTATGACAACCATTAGTTTGGCT,25,40,60.75
nCoV-2019_42_LEFT,nCoV-2019_2,TGCAAGAGATGGTTGTGTTCCC,22,50,61.08
nCoV-2019_42_RIGHT,nCoV-2019_2,CCTACCTCCCTTTGTTGTGTTGT,23,47.83,60.69
nCoV-2019_43_LEFT,nCoV-2019_1,TACGACAGATGTCTTGTGCTGC,22,50,60.93
nCoV-2019_43_RIGHT,nCoV-2019_1,AGCAGCATCTACAGCAAAAGCA,22,45.45,61.14
nCoV-2019_44_LEFT,nCoV-2019_2,TGCCACAGTACGTCTACAAGCT,22,50,61.66
nCoV-2019_44_RIGHT,nCoV-2019_2,AACCTTTCCACATACCGCAGAC,22,50,60.87
nCoV-2019_45_LEFT,nCoV-2019_1,TACCTACAACTTGTGCTAATGACCC,25,44,60.57
nCoV-2019_45_RIGHT,nCoV-2019_1,AAATTGTTTCTTCATGTTGGTAGTTAGAGA,30,30,60.01
nCoV-2019_46_LEFT,nCoV-2019_2,TGTCGCTTCCAAGAAAAGGACG,22,50,61.38
nCoV-2019_46_RIGHT,nCoV-2019_2,CACGTTCACCTAAGTTGGCGTA,22,50,60.86
nCoV-2019_47_LEFT,nCoV-2019_1,AGGACTGGTATGATTTTGTAGAAAACCC,28,39.29,61.42
nCoV-2019_47_RIGHT,nCoV-2019_1,AATAACGGTCAAAGAGTTTTAACCTCTC,28,35.71,60.06
nCoV-2019_48_LEFT,nCoV-2019_2,TGTTGACACTGACTTAACAAAGCCT,25,40,61.09
nCoV-2019_48_RIGHT,nCoV-2019_2,TAGATTACCAGAAGCAGCGTGC,22,50,60.74
nCoV-2019_49_LEFT,nCoV-2019_1,AGGAATTACTTGTGTATGCTGCTGA,25,40,60.57
nCoV-2019_49_RIGHT,nCoV-2019_1,TGACGATGACTTGGTTAGCATTAATACA,28,35.71,61.05
nCoV-2019_50_LEFT,nCoV-2019_2,GTTGATAAGTACTTTGATTGTTACGATGGT,30,33.33,60.59
nCoV-2019_50_RIGHT,nCoV-2019_2,TAACATGTTGTGCCAACCACCA,22,45.45,60.95
nCoV-2019_51_LEFT,nCoV-2019_1,TCAATAGCCGCCACTAGAGGAG,22,54.55,61.34
nCoV-2019_51_RIGHT,nCoV-2019_1,AGTGCATTAACATTGGCCGTGA,22,45.45,61.14
nCoV-2019_52_LEFT,nCoV-2019_2,CATCAGGAGATGCCACAACTGC,22,54.55,61.83
nCoV-2019_52_RIGHT,nCoV-2019_2,GTTGAGAGCAAAATTCATGAGGTCC,25,44,60.62
nCoV-2019_53_LEFT,nCoV-2019_1,AGCAAAATGTTGGACTGAGACTGA,24,41.67,60.69
nCoV-2019_53_RIGHT,nCoV-2019_1,AGCCTCATAAAACTCAGGTTCCC,23,47.83,60.31
nCoV-2019_54_LEFT,nCoV-2019_2,TGAGTTAACAGGACACATGTTAGACA,26,38.46,60.18
nCoV-2019_54_RIGHT,nCoV-2019_2,AACCAAAAACTTGTCCATTAGCACA,25,36,60.11
nCoV-2019_55_LEFT,nCoV-2019_1,ACTCAACTTTACTTAGGAGGTATGAGCT,28,39.29,61.43
nCoV-2019_55_RIGHT,nCoV-2019_1,GGTGTACTCTCCTATTTGTACTTTACTGT,29,37.93,60.54
nCoV-2019_56_LEFT,nCoV-2019_2,ACCTAGACCACCACTTAACCGA,22,50,60.49
nCoV-2019_56_RIGHT,nCoV-2019_2,ACACTATGCGAGCAGAAGGGTA,22,50,61.21
nCoV-2019_57_LEFT,nCoV-2019_1,ATTCTACACTCCAGGGACCACC,22,54.55,61.16
nCoV-2019_57_RIGHT,nCoV-2019_1,GTAATTGAGCAGGGTCGCCAAT,22,50,61.26
nCoV-2019_58_LEFT,nCoV-2019_2,TGATTTGAGTGTTGTCAATGCCAGA,25,40,61.44
nCoV-2019_58_RIGHT,nCoV-2019_2,CTTTTCTCCAAGCAGGGTTACGT,23,47.83,61.06
nCoV-2019_59_LEFT,nCoV-2019_1,TCACGCATGATGTTTCATCTGCA,23,43.48,61.42
nCoV-2019_59_RIGHT,nCoV-2019_1,AAGAGTCCTGTTACATTTTCAGCTTG,26,38.46,60.02
nCoV-2019_60_LEFT,nCoV-2019_2,TGATAGAGACCTTTATGACAAGTTGCA,27,37.04,60.53
nCoV-2019_60_RIGHT,nCoV-2019_2,GGTACCAACAGCTTCTCTAGTAGC,24,50,60.44
nCoV-2019_61_LEFT,nCoV-2019_1,TGTTTATCACCCGCGAAGAAGC,22,50,61.5
nCoV-2019_61_RIGHT,nCoV-2019_1,ATCACATAGACAACAGGTGCGC,22,50,61.25
nCoV-2019_62_LEFT,nCoV-2019_2,GGCACATGGCTTTGAGTTGACA,22,50,61.91
nCoV-2019_62_RIGHT,nCoV-2019_2,GTTGAACCTTTCTACAAGCCGC,22,50,60.35
nCoV-2019_63_LEFT,nCoV-2019_1,TGTTAAGCGTGTTGACTGGACT,22,45.45,60.16
nCoV-2019_63_RIGHT,nCoV-2019_1,ACAAACTGCCACCATCACAACC,22,50,61.85
nCoV-2019_64_LEFT,nCoV-2019_2,TCGATAGATATCCTGCTAATTCCATTGT,28,35.71,60.11
nCoV-2019_64_RIGHT,nCoV-2019_2,AGTCTTGTAAAAGTGTTCCAGAGGT,25,40,60.1
nCoV-2019_65_LEFT,nCoV-2019_1,GCTGGCTTTAGCTTGTGGGTTT,22,50,61.92
nCoV-2019_65_RIGHT,nCoV-2019_1,TGTCAGTCATAGAACAAACACCAATAGT,28,35.71,60.9
nCoV-2019_66_LEFT,nCoV-2019_2,GGGTGTGGACATTGCTGCTAAT,22,50,61.21
nCoV-2019_66_RIGHT,nCoV-2019_2,TCAATTTCCATTTGACTCCTGGGT,24,41.67,60.45
nCoV-2019_67_LEFT,nCoV-2019_1,GTTGTCCAACAATTACCTGAAACTTACT,28,35.71,60.43
nCoV-2019_67_RIGHT,nCoV-2019_1,CAACCTTAGAAACTACAGATAAATCTTGGG,30,36.67,60.4
nCoV-2019_68_LEFT,nCoV-2019_2,ACAGGTTCATCTAAGTGTGTGTGT,24,41.67,60.14
nCoV-2019_68_RIGHT,nCoV-2019_2,CTCCTTTATCAGAACCAGCACCA,23,47.83,60.31
nCoV-2019_69_LEFT,nCoV-2019_1,TGTCGCAAAATATACTCAACTGTGTCA,27,37.04,61.43
nCoV-2019_69_RIGHT,nCoV-2019_1,TCTTTATAGCCACGGAACCTCCA,23,47.83,61.14
nCoV-2019_70_LEFT,nCoV-2019_2,ACAAAAGAAAATGACTCTAAAGAGGGTTT,29,31.03,60.13
nCoV-2019_70_RIGHT,nCoV-2019_2,TGACCTTCTTTTAAAGACATAACAGCAG,28,35.71,60.27
nCoV-2019_71_LEFT,nCoV-2019_1,ACAAATCCAATTCAGTTGTCTTCCTATTC,29,34.48,60.54
nCoV-2019_71_RIGHT,nCoV-2019_1,TGGAAAAGAAAGGTAAGAACAAGTCCT,27,37.04,60.8
nCoV-2019_72_LEFT,nCoV-2019_2,ACACGTGGTGTTTATTACCCTGAC,24,45.83,61.04
nCoV-2019_72_RIGHT,nCoV-2019_2,ACTCTGAACTCACTTTCCATCCAAC,25,44,60.97
nCoV-2019_73_LEFT,nCoV-2019_1,CAATTTTGTAATGATCCATTTTTGGGTGT,29,31.03,60.29
nCoV-2019_73_RIGHT,nCoV-2019_1,CACCAGCTGTCCAACCTGAAGA,22,54.55,62.45
nCoV-2019_74_LEFT,nCoV-2019_2,ACATCACTAGGTTTCAAACTTTACTTGC,28,35.71,60.68
nCoV-2019_74_RIGHT,nCoV-2019_2,GCAACACAGTTGCTGATTCTCTTC,24,45.83,60.85
nCoV-2019_75_LEFT,nCoV-2019_1,AGAGTCCAACCAACAGAATCTATTGT,26,38.46,60.24
nCoV-2019_75_RIGHT,nCoV-2019_1,ACCACCAACCTTAGAATCAAGATTGT,26,38.46,60.69
nCoV-2019_76_LEFT,nCoV-2019_2,AGGGCAAACTGGAAAGATTGCT,22,45.45,60.76
nCoV-2019_76_RIGHT,nCoV-2019_2,ACACCTGTGCCTGTTAAACCAT,22,45.45,60.42
nCoV-2019_77_LEFT,nCoV-2019_1,CCAGCAACTGTTTGTGGACCTA,22,50,60.75
nCoV-2019_77_RIGHT,nCoV-2019_1,CAGCCCCTATTAAACAGCCTGC,22,54.55,61.59
nCoV-2019_78_LEFT,nCoV-2019_2,CAACTTACTCCTACTTGGCGTGT,23,47.83,60.55
nCoV-2019_78_RIGHT,nCoV-2019_2,TGTGTACAAAAACTGCCATATTGCA,25,36,60.22
nCoV-2019_79_LEFT,nCoV-2019_1,GTGGTGATTCAACTGAATGCAGC,23,47.83,60.92
nCoV-2019_79_RIGHT,nCoV-2019_1,CATTTCATCTGTGAGCAAAGGTGG,24,45.83,60.62
nCoV-2019_80_LEFT,nCoV-2019_2,TTGCCTTGGTGATATTGCTGCT,22,45.45,60.89
nCoV-2019_80_RIGHT,nCoV-2019_2,TGGAGCTAAGTTGTTTAACAAGCG,24,41.67,60.02
nCoV-2019_81_LEFT,nCoV-2019_1,GCACTTGGAAAACTTCAAGATGTGG,25,44,61.24
nCoV-2019_81_RIGHT,nCoV-2019_1,GTGAAGTTCTTTTCTTGTGCAGGG,24,45.83,60.73
nCoV-2019_82_LEFT,nCoV-2019_2,GGGCTATCATCTTATGTCCTTCCCT,25,48,61.52
nCoV-2019_82_RIGHT,nCoV-2019_2,TGCCAGAGATGTCACCTAAATCAA,24,41.67,60.02
nCoV-2019_83_LEFT,nCoV-2019_1,TCCTTTGCAACCTGAATTAGACTCA,25,40,60.46
nCoV-2019_83_RIGHT,nCoV-2019_1,TTTGACTCCTTTGAGCACTGGC,22,50,61.33
nCoV-2019_84_LEFT,nCoV-2019_2,TGCTGTAGTTGTCTCAAGGGCT,22,50,61.61
nCoV-2019_84_RIGHT,nCoV-2019_2,AGGTGTGAGTAAACTGTTACAAACAAC,27,37.04,60.36
nCoV-2019_85_LEFT,nCoV-2019_1,ACTAGCACTCTCCAAGGGTGTT,22,50,61.03
nCoV-2019_85_RIGHT,nCoV-2019_1,ACACAGTCTTTTACTCCAGATTCCC,25,44,60.51
nCoV-2019_86_LEFT,nCoV-2019_2,TCAGGTGATGGCACAACAAGTC,22,50,61.07
nCoV-2019_86_RIGHT,nCoV-2019_2,ACGAAAGCAAGAAAAAGAAGTACGC,25,40,61.01
nCoV-2019_87_LEFT,nCoV-2019_1,CGACTACTAGCGTGCCTTTGTA,22,50,60.16
nCoV-2019_87_RIGHT,nCoV-2019_1,ACTAGGTTCCATTGTTCAAGGAGC,24,45.83,60.81
nCoV-2019_88_LEFT,nCoV-2019_2,CCATGGCAGATTCCAACGGTAC,22,54.55,61.58
nCoV-2019_88_RIGHT,nCoV-2019_2,TGGTCAGAATAGTGCCATGGAGT,23,47.83,61.4
nCoV-2019_89_LEFT,nCoV-2019_1,GTACGCGTTCCATGTGGTCATT,22,50,61.5
nCoV-2019_89_RIGHT,nCoV-2019_1,ACCTGAAAGTCAACGAGATGAAACA,25,40,60.91
nCoV-2019_90_LEFT,nCoV-2019_2,ACACAGACCATTCCAGTAGCAGT,23,47.83,61.58
nCoV-2019_90_RIGHT,nCoV-2019_2,TGAAATGGTGAATTGCCCTCGT,22,45.45,60.82
nCoV-2019_91_LEFT,nCoV-2019_1,TCACTACCAAGAGTGTGTTAGAGGT,25,44,60.93
nCoV-2019_91_RIGHT,nCoV-2019_1,TTCAAGTGAGAACCAAAAGATAATAAGCA,29,31.03,60.03
nCoV-2019_92_LEFT,nCoV-2019_2,TTTGTGCTTTTTAGCCTTTCTGCT,24,37.5,60.14
nCoV-2019_92_RIGHT,nCoV-2019_2,AGGTTCCTGGCAATTAATTGTAAAAGG,27,37.04,60.53
nCoV-2019_93_LEFT,nCoV-2019_1,TGAGGCTGGTTCTAAATCACCCA,23,47.83,61.59
nCoV-2019_93_RIGHT,nCoV-2019_1,AGGTCTTCCTTGCCATGTTGAG,22,50,60.55
nCoV-2019_94_LEFT,nCoV-2019_2,GGCCCCAAGGTTTACCCAATAA,22,50,60.56
nCoV-2019_94_RIGHT,nCoV-2019_2,TTTGGCAATGTTGTTCCTTGAGG,23,43.48,60.18
nCoV-2019_95_LEFT,nCoV-2019_1,TGAGGGAGCCTTGAATACACCA,22,50,61.1
nCoV-2019_95_RIGHT,nCoV-2019_1,CAGTACGTTTTTGCCGAGGCTT,22,50,61.95
nCoV-2019_96_LEFT,nCoV-2019_2,GCCAACAACAACAAGGCCAAAC,22,50,61.82
nCoV-2019_96_RIGHT,nCoV-2019_2,TAGGCTCTGTTGGTGGGAATGT,22,50,61.36
nCoV-2019_97_LEFT,nCoV-2019_1,TGGATGACAAAGATCCAAATTTCAAAGA,28,32.14,60.22
nCoV-2019_97_RIGHT,nCoV-2019_1,ACACACTGATTAAAGATTGCTATGTGAG,28,35.71,60.17
nCoV-2019_98_LEFT,nCoV-2019_2,AACAATTGCAACAATCCATGAGCA,24,37.5,60.5
nCoV-2019_98_RIGHT,nCoV-2019_2,TTCTCCTAAGAAGCTATTAAAATCACATGG,30,33.33,60.01