Tool,Type,Status,Sequence Input format,Requires external dependencies,Notes,Available at
PriMux,Command line based,Abandoned,FASTA file,Yes,Incomplete documentation and lack of support complicate using this package,https://sourceforge.net/projects/primux/
PrimerDesign,Web-application,Not available,Web form or alignment file,No,Application is persistently off-line,https://www.hiv.lanl.gov/tools/primer/main
DECIPHER,R package,Available,SQLite connection object,Yes,Input format and dependency requirements complicate using this package,http://www2.decipher.codes/
PrimerMapper,GUI,Abandoned,FASTA file or NCBIÕs GenBank file,Yes,GUI is poorly suited for highly automated primer design,https://github.com/dohalloran/PrimerMapper
openPrimeR,R package,Available,FASTA or CSV file,For some features,Does not scale to large input data,https://www.bioconductor.org/packages/release/bioc/html/openPrimeR.html
Prider,R package,Available,FASTA file,No,-,https://github.com/tamminenlab/prider