LOCUS F2978_2J17_mu_1-2 1997 bp mRNA linear PRI 27-JAN-2022 DEFINITION Homo sapiens 2J17 monoclonal IgM antibody heavy chain mRNA, complete cds. ACCESSION F2978_2J17_mu_1-2 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1997) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1997) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (27-JAN-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:2. FEATURES Location/Qualifiers source 1..1997 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 67..1848 /codon_start=1 /product="immunoglobulin mu chain" /translation="MDWTWRILFLVAAATGAHSQVQLVQSGAEVKKPGASVKVSCKAS GYTFTGYYMHWVRQAPGQGLEWMGWINPNSGGTNYAQNFQGRVTMTRDTSISTVYMGL SRLRSDDTAVYYCARSNHYTNPVVRLDYWGQGTLVTVSSGSASAPTLFPLVSCENSPS DTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKD VMQGTDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQ ATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQS MFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTT YDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTD LPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQ RGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVT ERTVDKSTGKPTLYNVSLVMSDTAGTCY" BASE COUNT 456 a 644 c 543 g 354 t ORIGIN 1 gggggagcat cacccagcaa ccacatctgt cctctagaga atcccctgag agctccgttc 61 ctcaccatgg actggacctg gaggatcctc ttcttggtgg cagcagccac aggagcccac 121 tcccaggtgc agctggtgca gtctggggct gaggtgaaga agcctggggc ctcagtgaag 181 gtctcctgca aggcttctgg atacaccttc accggctact atatgcactg ggtgcgacag 241 gcccctggac aagggcttga gtggatggga tggatcaacc ctaacagtgg tggcacaaac 301 tatgcacaga actttcaggg cagggtcacc atgaccaggg acacgtccat cagcacagtc 361 tacatggggc tgagcaggct gagatctgac gacacggccg tatattactg tgcgaggtct 421 aaccactaca ctaaccccgt cgtgaggctt gactactggg gccagggaac cctggtcacc 481 gtctcctcag ggagtgcatc cgccccaacc cttttccccc tcgtctcctg tgagaattcc 541 ccgtcggata cgagcagcgt ggccgttggc tgcctcgcac aggacttcct tcccgactcc 601 atcactttct cctggaaata caagaacaac tctgacatca gcagcacccg gggcttccca 661 tcagtcctga gagggggcaa gtacgcagcc acctcacagg tgctgctgcc ttccaaggac 721 gtcatgcagg gcacagacga acacgtggtg tgcaaagtcc agcaccccaa cggcaacaaa 781 gaaaagaacg tgcctcttcc agtgattgcc gagctgcctc ccaaagtgag cgtcttcgtc 841 ccaccccgcg acggcttctt cggcaacccc cgcaagtcca agctcatctg ccaggccacg 901 ggtttcagtc cccggcagat tcaggtgtcc tggctgcgcg aggggaagca ggtggggtct 961 ggcgtcacca cggaccaggt gcaggctgag gccaaagagt ctgggcccac gacctacaag 1021 gtgaccagca cactgaccat caaagagagc gactggctca gccagagcat gttcacctgc 1081 cgcgtggatc acaggggcct gaccttccag cagaatgcgt cctccatgtg tgtccccgat 1141 caagacacag ccatccgggt cttcgccatc cccccatcct ttgccagcat cttcctcacc 1201 aagtccacca agttgacctg cctggtcaca gacctgacca cctatgacag cgtgaccatc 1261 tcctggaccc gccagaatgg cgaagctgtg aaaacccaca ccaacatctc cgagagccac 1321 cccaatgcca ctttcagcgc cgtgggtgag gccagcatct gcgaggatga ctggaattcc 1381 ggggagaggt tcacgtgcac cgtgacccac acagacctgc cctcgccact gaagcagacc 1441 atctcccggc ccaagggggt ggccctgcac aggcccgatg tctacttgct gccaccagcc 1501 cgggagcagc tgaacctgcg ggagtcggcc accatcacgt gcctggtgac gggcttctct 1561 cccgcggacg tcttcgtgca gtggatgcag agggggcagc ccttgtcccc ggagaagtat 1621 gtgaccagcg ccccaatgcc tgagccccag gccccaggcc ggtacttcgc ccacagcatc 1681 ctgaccgtgt ccgaagagga atggaacacg ggggagacct acacctgcgt ggtggcccat 1741 gaggccctgc ccaacagggt caccgagagg accgtggaca agtccaccgg taaacccacc 1801 ctgtacaacg tgtccctggt catgtccgac acagctggca cctgctactg accctgctgg 1861 cctgcccaca ggctcggggc ggctggccgc tctgtgtgtg catgcaaact aaccgtgtca 1921 acggggtgag atgttgcatc ttataaaatt agaaataaaa agatccattc aaaaaaaaaa 1981 aaaaaaaaaa aaaaaaa // LOCUS F2978_2J17_kappa_1-33 954 bp mRNA linear PRI 27-JAN-2022 DEFINITION Homo sapiens 2J17 monoclonal IgM antibody kappa chain mRNA, complete cds. ACCESSION F2978_2J17_kappa_1-33 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 954) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 954) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (27-JAN-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:2. FEATURES Location/Qualifiers source 1..954 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 30..743 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MDMRVPAQLLGLLLLWLSGARCDIQMTQSPSSLSASVGDRVTIT CQASQDISNYLNWYQQKPGKAPKLLIYDASNLDTGVPSRFSGSGSGTDFTFTISSLQP EDIATYYCQQYDNLPPKLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLL NNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYAC EVTHQGLSSPVTKSFNRGEC" BASE COUNT 246 a 277 c 221 g 210 t ORIGIN 1 gggggagtca gtcccaacca ggacacagca tggacatgag ggtccctgct cagctcctgg 61 ggctcctgct gctctggctc tcaggtgcca gatgtgacat ccagatgacc cagtctccat 121 cctccctgtc tgcatctgta ggagacagag tcaccatcac ttgccaggcg agtcaagaca 181 ttagcaacta tttaaattgg tatcagcaga aaccagggaa agcccctaag ctcctgatct 241 acgatgcatc caatttggac acaggggtcc catcaaggtt cagtggaagt ggatctggga 301 cagattttac tttcaccatc agcagcctgc agcctgaaga tattgcaaca tattactgtc 361 aacagtatga taatctccct ccaaagctca ctttcggcgg agggaccaag gtggagatca 421 aacgaactgt ggctgcacca tctgtcttca tcttcccgcc atctgatgag cagttgaaat 481 ctggaactgc ctctgttgtg tgcctgctga ataacttcta tcccagagag gccaaagtac 541 agtggaaggt ggataacgcc ctccaatcgg gtaactccca ggagagtgtc acagagcagg 601 acagcaagga cagcacctac agcctcagca gcaccctgac gctgagcaaa gcagactacg 661 agaaacacaa agtctacgcc tgcgaagtca cccatcaggg cctgagctcg cccgtcacaa 721 agagcttcaa caggggagag tgttagaggg agaagtgccc ccacctgctc ctcagttcca 781 gcctgacccc ctcccatcct ttggcctctg accctttttc cacaggggac ctacccctat 841 tgcggtcctc cagctcatct ttcacctcac ccccctcctc ctccttggct ttaattatgc 901 taatgttgga ggagaatgaa taaataaagt gaatctttgc acctacgtaa aaaa // LOCUS F3015_1B17_alpha_1-69 1482 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1B17 monoclonal IgA antibody heavy chain mRNA, complete cds. ACCESSION F3015_1B17_alpha_1-69 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1482) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1482) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1482 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1479 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDWTWRFLFVVAAATGVQSQVQLVQSGAEVKKPGSSVKVSCKAS GGTFSNYAVSWVRQAPGQGLEWMGGTVPIFGTPNYAQKFQGRVTITADESTSTAYMEL NSLRSEDTAMYYCATWSDVDTDMFFDYWGPGTLVTVSSASPTSPKVFPLSLCSTQPDG NVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPATQCL AGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALED LLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCAE PWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCLA RGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDT FSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY" BASE COUNT 298 a 492 c 424 g 268 t ORIGIN 1 atggactgga cctggaggtt cctctttgtg gtggcagcag ctacaggtgt ccagtcccag 61 gtgcagctgg tgcagtctgg ggctgaggtg aagaagcctg ggtcctcggt gaaggtctcc 121 tgcaaggctt ctggaggcac cttcagcaac tatgctgtca gctgggtgcg acaggcccct 181 ggacaagggc ttgagtggat gggagggacc gtccctatct ttggtacacc aaattacgca 241 cagaagttcc agggcagagt cacgattacc gcggacgaat ccacgagcac agcctacatg 301 gagctgaaca gcctgagatc tgaggacacg gccatgtatt actgtgcgac atggagtgac 361 gtggatacag atatgttttt tgactactgg ggcccgggaa ccctggtcac cgtctcctca 421 gcatccccga ccagccccaa ggtcttcccg ctgagcctct gcagcaccca gccagatggg 481 aacgtggtca tcgcctgcct ggtccagggc ttcttccccc aggagccact cagtgtgacc 541 tggagcgaaa gcggacaggg cgtgaccgcc agaaacttcc cacccagcca ggatgcctcc 601 ggggacctgt acaccacgag cagccagctg accctgccgg ccacacagtg cctagccggc 661 aagtccgtga catgccacgt gaagcactac acgaatccca gccaggatgt gactgtgccc 721 tgcccagttc cctcaactcc acctacccca tctccctcaa ctccacctac cccatctccc 781 tcatgctgcc acccccgact gtcactgcac cgaccggccc tcgaggacct gctcttaggt 841 tcagaagcga acctcacgtg cacactgacc ggcctgagag atgcctcagg tgtcaccttc 901 acctggacgc cctcaagtgg gaagagcgct gttcaaggac cacctgagcg tgacctctgt 961 ggctgctaca gcgtgtccag tgtcctgccg ggctgtgccg agccatggaa ccatgggaag 1021 accttcactt gcactgctgc ctaccccgag tccaagaccc cgctaaccgc caccctctca 1081 aaatccggaa acacattccg gcccgaggtc cacctgctgc cgccgccgtc ggaggagctg 1141 gccctgaacg agctggtgac gctgacgtgc ctggcacgcg gcttcagccc caaggacgtg 1201 ctggttcgct ggctgcaggg gtcacaggag ctgccccgcg agaagtacct gacttgggca 1261 tcccggcagg agcccagcca gggcaccacc accttcgctg tgaccagcat actgcgcgtg 1321 gcagccgagg actggaagaa gggggacacc ttctcctgca tggtgggcca cgaggccctg 1381 ccgctggcct tcacacagaa gaccatcgac cgcttggcgg gtaaacccac ccatgtcaat 1441 gtgtctgttg tcatggcgga ggtggacggc acctgctact ga // LOCUS F3015_1B17_kappa_3-11 708 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1B17 monoclonal IgA antibody kappa chain mRNA, complete cds. ACCESSION F3015_1B17_kappa_3-11 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 708) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 708) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..708 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..705 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MEAPAQLLFLLLLWLPDTTGEIVLTQSPATLSLSPGERATLSCR ASQSVSSFLAWYQQKPGQTPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPED VAVYYCQQRSNWPPYTFGQGTNLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNF YPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC" BASE COUNT 176 a 212 c 174 g 146 t ORIGIN 1 atggaagccc cagctcagct tctcttcctc ctgctactct ggctcccaga taccaccgga 61 gaaattgtgt tgacacagtc tccagccacc ctgtctttgt ctccagggga aagagccacc 121 ctctcctgca gggccagtca gagtgttagc agcttcttag cctggtacca acagaaacct 181 ggccagactc ccaggctcct catctatgat gcatccaata gggccactgg catcccagcc 241 aggttcagtg gcagtgggtc tgggacagac ttcactctca ccatcagcag cctagagcct 301 gaagatgttg cagtttatta ctgtcagcag cgtagcaact ggcctccgta cacttttggc 361 caggggacca acctggagat caaacgaact gtggctgcac catctgtctt catcttcccg 421 ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 481 tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc 541 caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg 601 acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag 661 ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgttag // LOCUS F3005_3L11_mu_4-59 1928 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3L11 monoclonal IgM antibody heavy chain mRNA, complete cds. ACCESSION F3005_3L11_mu_4-59 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1928) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1928) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1928 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 41..1801 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MKHLWFFLLLVAAPRWVLSQVQLQESGPGLVKPSETLSLTCTVS GGSISSYYWTWIRQSPGKGLECIGFIYYSGSTNYNPSLKSRVTISVDTSKNHFSLKLG SVTAADTAVYYCARGTSYWSYWGQGTLVTVSSGSASAPTLFPLVSCENSPSDTSSVAV GCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDE HVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPR QIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVD HRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTIS WTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQ TISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSP EKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKS TGKPTLYNVSLVMSDTAGTCY" BASE COUNT 414 a 634 c 519 g 361 t ORIGIN 1 gaaatacttt ctgagagtcc tggacctcct gtgcaagaac atgaaacatc tgtggttctt 61 ccttctcctg gtggcagctc ccagatgggt cctgtcccag gtgcagctgc aggagtcggg 121 cccaggactg gtgaagcctt cggagaccct gtccctcacc tgcactgtct ctggtggctc 181 catcagtagt tactactgga cctggatccg gcagtcccca gggaagggac tggagtgcat 241 tgggtttatc tattacagtg ggagcaccaa ctacaacccc tccctcaaga gtcgagtcac 301 catatcagta gacacgtcca agaaccactt ctccctgaag ctgggctctg tgaccgctgc 361 ggacacggcc gtgtattatt gtgcgagagg tacatcctac tggtcctact ggggccaggg 421 aaccctggtc accgtctcct cagggagtgc atccgcccca acccttttcc ccctcgtctc 481 ctgtgagaat tccccgtcgg atacgagcag cgtggccgtt ggctgcctcg cacaggactt 541 ccttcccgac tccatcactt tctcctggaa atacaagaac aactctgaca tcagcagcac 601 ccggggcttc ccatcagtcc tgagaggggg caagtacgca gccacctcac aggtgctgct 661 gccttccaag gacgtcatgc agggcacaga cgaacacgtg gtgtgcaaag tccagcaccc 721 caacggcaac aaagaaaaga acgtgcctct tccagtgatt gccgagctgc ctcccaaagt 781 gagcgtcttc gtcccacccc gcgacggctt cttcggcaac ccccgcaagt ccaagctcat 841 ctgccaggcc acgggtttca gtccccggca gattcaggtg tcctggctgc gcgaggggaa 901 gcaggtgggg tctggcgtca ccacggacca ggtgcaggct gaggccaaag agtctgggcc 961 cacgacctac aaggtgacca gcacactgac catcaaagag agcgactggc tcagccagag 1021 catgttcacc tgccgcgtgg atcacagggg cctgaccttc cagcagaatg cgtcctccat 1081 gtgtgtcccc gatcaagaca cagccatccg ggtcttcgcc atccccccat cctttgccag 1141 catcttcctc accaagtcca ccaagttgac ctgcctggtc acagacctga ccacctatga 1201 cagcgtgacc atctcctgga cccgccagaa tggcgaagct gtgaaaaccc acaccaacat 1261 ctccgagagc caccccaatg ccactttcag cgccgtgggt gaggccagca tctgcgagga 1321 tgactggaat tccggggaga ggttcacgtg caccgtgacc cacacagacc tgccctcgcc 1381 actgaagcag accatctccc ggcccaaggg ggtggccctg cacaggcccg atgtctactt 1441 gctgccacca gcccgggagc agctgaacct gcgggagtcg gccaccatca cgtgcctggt 1501 gacgggcttc tctcccgcgg acgtcttcgt gcagtggatg cagagggggc agcccttgtc 1561 cccggagaag tatgtgacca gcgccccaat gcctgagccc caggccccag gccggtactt 1621 cgcccacagc atcctgaccg tgtccgaaga ggaatggaac acgggggaga cctacacctg 1681 cgtggtggcc catgaggccc tgcccaacag ggtcaccgag aggaccgtgg acaagtccac 1741 cggtaaaccc accctgtaca acgtgtccct ggtcatgtcc gacacagctg gcacctgcta 1801 ctgaccctgc tggcctgccc acaggctcgg ggcggctggc cgctctgtgt gtgcatgcaa 1861 actaaccgtg tcaacggggt gagatgttgc atcttataaa attagaaata aaaagatcca 1921 tccaaaaa // LOCUS F3005_3L11_kappa_1-5 826 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3L11 monoclonal IgM antibody kappa chain mRNA, complete cds. ACCESSION F3005_3L11_kappa_1-5 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 826) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 826) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..826 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 25..732 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MDMRVPAQLLGLLLLWLPGAKCDIQMTQSPSTLSASVGDRVTIT CRASQSISSWLAWYRQKPGKAPKLLIYKASSLESGVPSRFSGSGSGTEFTLTISSLQP DDFATYYCQQYNSYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNN FYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEV THQGLSSPVTKSFNRGEC" BASE COUNT 204 a 244 c 207 g 171 t ORIGIN 1 agtcagaccc agtcaggaca cagcatggac atgagggtcc ccgctcagct cctggggctc 61 ctgctgctct ggctcccagg tgccaaatgt gacatccaga tgacccagtc tccttccacc 121 ctgtctgcat ctgtaggaga cagagtcacc atcacttgcc gggccagtca gagtattagt 181 agttggttgg cctggtatcg gcagaaacca gggaaagccc ctaagctcct gatctataag 241 gcgtctagtt tagaaagtgg ggtcccatca aggttcagcg gcagtggatc tgggacagaa 301 ttcactctca ccatcagcag cctgcagcct gatgattttg caacttatta ctgccaacag 361 tataatagtt atccgtggac gttcggccaa gggaccaagg tggaaatcaa acgaactgtg 421 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgcc 481 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg 541 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac 601 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa 661 gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac 721 aggggagagt gttagaggga gaagtgcccc cacctgctcc tcagttccag cctgaccccc 781 tcccatcccc ttggcctctg accctttttc cacaggggac ctaccc // LOCUS F3005_2E14_mu_3-15 1998 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2E14 monoclonal IgM antibody heavy chain mRNA, complete cds. ACCESSION F3005_2E14_mu_3-15 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1998) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1998) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1998 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 83..1897 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWIFLGAILKGVQCEVQLVESGGGLVKPGGSLRLSCAAS GFTFSNAWMSWVRQAPGKGLEWVGRIKSKTDGGTTDYAAPVKGRFTISRDDSKNTLYL QMNSLKTEDTAVYYCIPRPSWYCSGDNCPSYGSAAEHDYWGQGTLVTVSSGSASAPTL FPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYA ATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFF GNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTL TIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKST KLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSG ERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGF SPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCV VAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY" BASE COUNT 432 a 628 c 560 g 378 t ORIGIN 1 ggagctctgg gagaggagcc ccagccttgg gattcccaag tgttttcatt cagtgatcag 61 gactgaacac agaggactca ccatggagtt tgggctgagc tggattttcc ttggtgctat 121 tttaaaaggt gtccagtgtg aggtgcagct ggtggagtct gggggaggct tggtaaagcc 181 tggggggtcc cttagactct cctgtgcagc ctctggattc actttcagta acgcctggat 241 gagttgggtc cgccaggctc cagggaaggg gctggagtgg gttggccgta ttaaaagcaa 301 aactgatggt gggacaacag actacgctgc acccgtgaaa ggcagattca ccatctcaag 361 agatgattca aaaaacacgc tgtatctgca aatgaacagc ctgaaaaccg aggacacagc 421 cgtgtattac tgtatcccac gaccctcttg gtattgtagt ggtgataact gcccgtccta 481 cgggtccgcg gccgaacatg actactgggg ccagggaacc ctggtcaccg tctcctcagg 541 gagtgcatcc gccccaaccc ttttccccct cgtctcctgt gagaattccc cgtcggatac 601 gagcagcgtg gccgttggct gcctcgcaca ggacttcctt cccgactcca tcactttctc 661 ctggaaatac aagaacaact ctgacatcag cagcacccgg ggcttcccat cagtcctgag 721 agggggcaag tacgcagcca cctcacaggt gctgctgcct tccaaggacg tcatgcaggg 781 cacagacgaa cacgtggtgt gcaaagtcca gcaccccaac ggcaacaaag aaaagaacgt 841 gcctcttcca gtgattgccg agctgcctcc caaagtgagc gtcttcgtcc caccccgcga 901 cggcttcttc ggcaaccccc gcaagtccaa gctcatctgc caggccacgg gtttcagtcc 961 ccggcagatt caggtgtcct ggctgcgcga ggggaagcag gtggggtctg gcgtcaccac 1021 ggaccaggtg caggctgagg ccaaagagtc tgggcccacg acctacaagg tgaccagcac 1081 actgaccatc aaagagagcg actggctcag ccagagcatg ttcacctgcc gcgtggatca 1141 caggggcctg accttccagc agaatgcgtc ctccatgtgt gtccccgatc aagacacagc 1201 catccgggtc ttcgccatcc ccccatcctt tgccagcatc ttcctcacca agtccaccaa 1261 gttgacctgc ctggtcacag acctgaccac ctatgacagc gtgaccatct cctggacccg 1321 ccagaatggc gaagctgtga aaacccacac caacatctcc gagagccacc ccaatgccac 1381 tttcagcgcc gtgggtgagg ccagcatctg cgaggatgac tggaattccg gggagaggtt 1441 cacgtgcacc gtgacccaca cagacctgcc ctcgccactg aagcagacca tctcccggcc 1501 caagggggtg gccctgcaca ggcccgatgt ctacttgctg ccaccagccc gggagcagct 1561 gaacctgcgg gagtcggcca ccatcacgtg cctggtgacg ggcttctctc ccgcggacgt 1621 cttcgtgcag tggatgcaga gggggcagcc cttgtccccg gagaagtatg tgaccagcgc 1681 cccaatgcct gagccccagg ccccaggccg gtacttcgcc cacagcatcc tgaccgtgtc 1741 cgaagaggaa tggaacacgg gggagaccta cacctgcgtg gtggcccatg aggccctgcc 1801 caacagggtc accgagagga ccgtggacaa gtccaccggt aaacccaccc tgtacaacgt 1861 gtccctggtc atgtccgaca cagctggcac ctgctactga ccctgctggc ctgcccacag 1921 gctcggggcg gctggccgct ctgtgtgtgc atgcaaacta accgtgtcaa cggggtgaga 1981 tgttgcatct tataaaat // LOCUS F3005_2E14_lambda_3-1 856 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2E14 monoclonal IgM antibody lambda chain mRNA, complete cds. ACCESSION F3005_2E14_lambda_3-1 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 856) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 856) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..856 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 40..732 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWIPLFLGVLAYCTGSVASYELTQPPSVSVSPGQTASITCSGD KLGDKYASWYQQKPGQSPVVVIYQDTKRPSGIPERFSGSNSGNTATLTISVTQAMDEA DYYCQAWDSSTEAFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYP GAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEG STVEKTVAPTECS" BASE COUNT 200 a 274 c 227 g 155 t ORIGIN 1 ggctgggcct caggaagcag catcggaggt gcctcagcca tggcatggat ccctctcttc 61 ctcggcgtcc ttgcttactg cacaggatcc gtggcctcct atgagctgac tcagccaccc 121 tcagtgtccg tgtccccagg acagacagcc agcatcacct gctctggaga taaattgggg 181 gataaatatg cttcctggta tcagcagaag ccaggccagt cccctgtggt ggtcatctat 241 caagatacca agcggccctc agggatccct gagcgattct ctggctccaa ctctgggaac 301 acagccactc tgaccatcag cgtgacccag gctatggatg aggctgacta ttactgtcag 361 gcgtgggaca gcagcactga ggcgttcggc ggagggacca agctgaccgt cctaggtcag 421 cccaaggctg ccccctcggt cactctgttc ccgccctcct ctgaggagct tcaagccaac 481 aaggccacac tggtgtgtct cataagtgac ttctacccgg gagccgtgac agtggcctgg 541 aaggcagata gcagccccgt caaggcggga gtggagacca ccacaccctc caaacaaagc 601 aacaacaagt acgcggccag cagctatctg agcctgacgc ctgagcagtg gaagtcccac 661 agaagctaca gctgccaggt cacgcatgaa gggagcaccg tggagaagac agtggcccct 721 acagaatgtt cataggttct aaaccctcac cccccccacg ggagactaga gctgcaggat 781 cccaggggag gggtctctcc tcccacccca aggcatcaag cccttctccc tgcactcaat 841 aaaccctcaa taaata // LOCUS F2978_1G22_alpha_1-69 1485 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1G22 monoclonal IgA antibody heavy chain mRNA, complete cds. ACCESSION F2978_1G22_alpha_1-69 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1485) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1485) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1485 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1482 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDWTWRFLFVVAAATGVQSQVQLVQSGAEVKKPGSSVKVSCKAS GGTSSSYAISWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVAVTADESTNTAYMEL SSLRSEDTAVYYCAITSDYDSSGYYSDYWGQGTLVTVSSASPTSPKVFPLSLCSTQPD GNVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPATQC LAGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALE DLLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCA EPWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCL ARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGD TFSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY" BASE COUNT 299 a 495 c 424 g 267 t ORIGIN 1 atggactgga cctggaggtt cctctttgtg gtggcagcag ctacaggtgt ccagtcccag 61 gtgcagctgg tgcagtctgg ggctgaggtg aagaagcctg ggtcctcggt gaaggtctcc 121 tgcaaggctt ctggaggcac ctccagcagc tatgctatca gctgggtgcg acaggcccct 181 ggacaagggc ttgagtggat gggagggatc atccctatct ttggtacagc aaactacgca 241 cagaagttcc agggcagagt cgcggttacc gcggacgaat ccacaaacac agcctacatg 301 gagctgagca gcctgagatc tgaggacacg gccgtgtatt actgtgcgat cacctccgac 361 tatgatagta gtggttatta cagtgactac tggggccagg gaaccctggt caccgtctcc 421 tcagcatccc cgaccagccc caaggtcttc ccgctgagcc tctgcagcac ccagccagat 481 gggaacgtgg tcatcgcctg cctggtccag ggcttcttcc cccaggagcc actcagtgtg 541 acctggagcg aaagcggaca gggcgtgacc gccagaaact tcccacccag ccaggatgcc 601 tccggggacc tgtacaccac gagcagccag ctgaccctgc cggccacaca gtgcctagcc 661 ggcaagtccg tgacatgcca cgtgaagcac tacacgaatc ccagccagga tgtgactgtg 721 ccctgcccag ttccctcaac tccacctacc ccatctccct caactccacc taccccatct 781 ccctcatgct gccacccccg actgtcactg caccgaccgg ccctcgagga cctgctctta 841 ggttcagaag cgaacctcac gtgcacactg accggcctga gagatgcctc aggtgtcacc 901 ttcacctgga cgccctcaag tgggaagagc gctgttcaag gaccacctga gcgtgacctc 961 tgtggctgct acagcgtgtc cagtgtcctg ccgggctgtg ccgagccatg gaaccatggg 1021 aagaccttca cttgcactgc tgcctacccc gagtccaaga ccccgctaac cgccaccctc 1081 tcaaaatccg gaaacacatt ccggcccgag gtccacctgc tgccgccgcc gtcggaggag 1141 ctggccctga acgagctggt gacgctgacg tgcctggcac gcggcttcag ccccaaggac 1201 gtgctggttc gctggctgca ggggtcacag gagctgcccc gcgagaagta cctgacttgg 1261 gcatcccggc aggagcccag ccagggcacc accaccttcg ctgtgaccag catactgcgc 1321 gtggcagccg aggactggaa gaagggggac accttctcct gcatggtggg ccacgaggcc 1381 ctgccgctgg ccttcacaca gaagaccatc gaccgcttgg cgggtaaacc cacccatgtc 1441 aatgtgtctg ttgtcatggc ggaggtggac ggcacctgct actga // LOCUS F2978_1G22_kappa_3-11 934 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1G22 monoclonal IgA antibody kappa chain mRNA, complete cds. ACCESSION F2978_1G22_kappa_3-11 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 934) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 934) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..934 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 35..742 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MEAPAQLLFLLLLWLPDTTGEIVLTQSPATLSLSPGERATLSCR ASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPED FAVYYCHQRSNWPPMYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNN FYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEV THQGLSSPVTKSFNRGEC" BASE COUNT 225 a 288 c 220 g 201 t ORIGIN 1 ggaggaactg ctcagttagg acccagaggg aaccatggaa gccccagctc agcttctctt 61 cctcctgcta ctctggctcc cagataccac cggagaaatt gtgttgacac agtctccagc 121 caccctgtct ttgtctccag gggaaagagc caccctctcc tgcagggcca gtcagagtgt 181 tagcagctac ttagcctggt accaacagaa acctggccag gctcccaggc tcctcatcta 241 tgatgcatcc aacagggcca ctggcatccc agccaggttc agtggcagtg ggtctgggac 301 agacttcact ctcaccatca gcagcctaga gcctgaagat tttgcagttt attactgtca 361 ccagcgtagc aactggcctc cgatgtacac ttttggccag gggaccaagc tggagatcaa 421 acgaactgtg gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc 481 tggaactgcc tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca 541 gtggaaggtg gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga 601 cagcaaggac agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga 661 gaaacacaaa gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa 721 gagcttcaac aggggagagt gttagaggga gaagtgcccc cacctgctcc tcagttccag 781 cctgaccccc tcccatcctt tggcctctga ccctttttcc acaggggacc tacccctatt 841 gcggtcctcc agctcatctt tcacctcacc cccctcctcc tccttggctt taattatgct 901 aatgttggag gagaatgaat aaataaagtg aatc // LOCUS F3015_3M13_alpha_3-30 1482 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3M13 monoclonal IgA antibody heavy chain mRNA, complete cds. ACCESSION F3015_3M13_alpha_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1482) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1482) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1482 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1479 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWVFLVALLRGGQCQVQLVESGGGVVQPGRSLRLSCAAS GFTFSSYAIHWVRQAPGKGLEWVAVISYDGSNKYYPDAVKGRFTISRDNSKNTLYLQM NSLRTEDTAVYYCAKPRSGNYHDAFDIWGQGTVVTVSSASPTSPKVFPLSLCSTQPDG NVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPATQCL AGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALED LLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLPGCAE PWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCLA RGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDT FSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY" BASE COUNT 304 a 480 c 418 g 280 t ORIGIN 1 atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgg ccagtgtcag 61 gtgcagctgg tagagtctgg gggaggcgtg gtccagcctg ggaggtccct gagactctcc 121 tgtgcagcct ctggattcac ctttagtagt tatgccatac actgggtccg ccaggctcca 181 ggcaaggggc tggagtgggt ggcagttata tcatatgatg gaagtaataa atactatcca 241 gacgccgtga agggccgatt caccatctcc agagacaatt ccaagaacac gctgtatctg 301 caaatgaaca gcctgagaac tgaggacacg gctgtgtatt actgtgcgaa accgaggagt 361 gggaactacc atgatgcttt tgatatctgg ggccaaggga cagtagtcac cgtctcttca 421 gcatccccga ccagccccaa ggtcttcccg ctgagcctct gcagcaccca gccagatggg 481 aacgtggtca tcgcctgcct ggtccagggc ttcttccccc aggagccact cagtgtgacc 541 tggagcgaaa gcggacaggg cgtgaccgcc agaaacttcc cacccagcca ggatgcctcc 601 ggggacctgt acaccacgag cagccagctg accctgccgg ccacacagtg cctagccggc 661 aagtccgtga catgccacgt gaagcactac acgaatccca gccaggatgt gactgtgccc 721 tgcccagttc cctcaactcc acctacccca tctccctcaa ctccacctac cccatctccc 781 tcatgctgcc acccccgact gtcactgcac cgaccggccc tcgaggacct gctcttaggt 841 tcagaagcga acctcacgtg cacactgacc ggcctgagag atgcctcagg tgtcaccttc 901 acctggacgc cctcaagtgg gaagagcgct gttcaaggac cacctgagcg tgacctctgt 961 ggctgctaca gcgtgtccag tgtcctgccg ggctgtgccg agccatggaa ccatgggaag 1021 accttcactt gcactgctgc ctaccccgag tccaagaccc cgctaaccgc caccctctca 1081 aaatccggaa acacattccg gcccgaggtc cacctgctgc cgccgccgtc ggaggagctg 1141 gccctgaacg agctggtgac gctgacgtgc ctggcacgcg gcttcagccc caaggacgtg 1201 ctggttcgct ggctgcaggg gtcacaggag ctgccccgcg agaagtacct gacttgggca 1261 tcccggcagg agcccagcca gggcaccacc accttcgctg tgaccagcat actgcgcgtg 1321 gcagccgagg actggaagaa gggggacacc ttctcctgca tggtgggcca cgaggccctg 1381 ccgctggcct tcacacagaa gaccatcgac cgcttggcgg gtaaacccac ccatgtcaat 1441 gtgtctgttg tcatggcgga ggtggacggc acctgctact ga // LOCUS F3015_3M13_kappa_3-20 825 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3M13 monoclonal IgA antibody kappa chain mRNA, complete cds. ACCESSION F3015_3M13_kappa_3-20 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 825) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 825) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..825 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 31..732 /codon_start=1 /product="immunoglobulin kappa chain" /translation="METPAQLLFLLLLWLPDTTGEIVLTQSPGTLSLSPGERATLSCR ASQSVSSSYLAWYQQKPGQAPRLLIYGASNRATGIPDRFSGSGSGTDFTLTISRLEPE DFAVYYCQQYGSSYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFY PREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTH QGLSSPVTKSFNRGEC" BASE COUNT 197 a 252 c 208 g 168 t ORIGIN 1 gagctgctca gttaggaccc agagggaacc atggaaaccc cagcgcagct tctcttcctc 61 ctgctactct ggctcccaga taccaccgga gaaattgtgt tgacgcagtc tccaggcacc 121 ctgtctttgt ctccagggga aagagccacc ctctcctgca gggccagtca gagtgttagc 181 agcagctact tagcctggta ccagcagaaa cctggccagg ctcccaggct cctcatctat 241 ggtgcatcca acagggccac tggcatccca gacaggttca gtggcagtgg gtctgggaca 301 gacttcactc tcaccatcag cagactggag cctgaagatt ttgcagtgta ttactgtcag 361 cagtatggta gctcgtacac ttttggccag gggaccaagc tggagatcaa acgaactgtg 421 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgcc 481 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg 541 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac 601 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa 661 gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac 721 aggggagagt gttagaggga gaagtgcccc cacctgctcc tcagttccag cctgaccccc 781 tcccatcctt tggcctctga ccctttttcc acaggggacc taccc // LOCUS F3015_2K21_gamma1_4-4 1532 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2K21 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3015_2K21_gamma1_4-4 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1532) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1532) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1532 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 25..1407 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MKHLWFFLLLVAAPRWVLSQVHLQESGPGLVKPSETLVLTCTVS GGSISNYYWSWIRQSAGKGLEYIGRIYTSGSTDYNPSLKSRVTMSVDTSKNQFSLKLN SVTAADTAMYYCAMGFDPWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLV KDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNH KPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYK CKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIA VEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHY TQKSLSLSPGK" BASE COUNT 342 a 516 c 407 g 267 t ORIGIN 1 ctcatggacc tcctgcacaa gaacatgaaa cacctgtggt tcttcctcct gctggtggca 61 gctcccagat gggtcctgtc ccaggtgcac ctgcaggagt cgggcccagg actggtgaag 121 ccttcggaga ccctggtcct cacctgcact gtctctggtg gctccattag taattattat 181 tggagctgga tccggcagtc cgccgggaag ggactggagt acattgggcg tatctatacc 241 agtgggagca ccgactacaa cccctccctc aagagtcgag tcaccatgtc agtagacacg 301 tccaagaacc agttctccct gaagctgaac tctgtgaccg ccgcggacac ggccatgtat 361 tactgtgcga tggggttcga cccctggggc cagggaaccc tggtcaccgt ctcctcagcc 421 tccaccaagg gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc 481 acagcggccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 541 aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 601 ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac 661 atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagagagt tgagcccaaa 721 tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg 781 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 841 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac 901 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc 961 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag 1021 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa 1081 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 1141 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc 1201 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg 1261 gactccgacg gctccttctt cctctatagc aagctcaccg tggacaagag caggtggcag 1321 caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1381 aagagcctct ccctgtcccc gggtaaatga gtgcgacggc cggcaagccc ccgctccccg 1441 ggctctcgcg gtcgcacgag gatgcttggc acgtaccccg tctacatact tcccaggcac 1501 ccagcatgga aataaagcac ccaccactgc cc // LOCUS F3015_2K21_kappa_3-20 949 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2K21 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F3015_2K21_kappa_3-20 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 949) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 949) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..949 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 37..741 /codon_start=1 /product="immunoglobulin kappa chain" /translation="METPAQLLFLLLLWLPDTTGETVLTQSPGTLSLSPGERATLSCR ASQSVSSIYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPE DFAVYYCQQYGSSPWTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNF YPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC" BASE COUNT 236 a 285 c 228 g 200 t ORIGIN 1 ggggaagagc tgctcagtta ggacccagag ggaaccatgg aaaccccagc gcagcttctc 61 ttcctcctgc tactctggct cccagatacc accggagaaa ctgtgttgac gcagtctcca 121 ggcaccctgt ctttgtctcc aggggaaaga gccaccctct cctgcagggc cagtcagagt 181 gttagcagca tctacttagc ctggtaccag caaaaacctg gccaggctcc caggctcctc 241 atctatggtg catccagcag ggccactggc atcccagaca gattcagtgg cagtgggtct 301 gggacagact tcactctcac catcagcaga ctggagcctg aagattttgc agtgtattac 361 tgtcagcagt atggtagctc accctggacg ttcggccaag ggaccaagct ggaaatcaaa 421 cgaactgtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct 481 ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag 541 tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac 601 agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag 661 aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag 721 agcttcaaca ggggagagtg ttagagggag aagtgccccc acctgctcct cagttccagc 781 ctgaccccct cccatccttt ggcctctgac cctttttcca caggggacct acccctattg 841 cggtcctcca gctcatcttt cacctcaccc ccctcctcct ccttggcttt aattatgcta 901 atgttggagg agaatgaata aataaagtga atctttgcga aaaaaaaaa // LOCUS F3005_3N8_mu_3-23 1978 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3N8 monoclonal IgM antibody heavy chain mRNA, complete cds. ACCESSION F3005_3N8_mu_3-23 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1978) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1978) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1978 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 82..1851 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWLFLVAILKGVQCEVQLLESGGGSVQPGGSLRLSCVPS GFAFSYYAMSWVRQAPGKGLEWVSTISGGGSTEYADSVKGRFTISRDNSKNTLYLQMN SLRAEDTAVYYCARGGGPYWYFDLWGRGTLVTVSSGSASAPTLFPLVSCENSPSDTSS VAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQG TDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGF SPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTC RVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSV TISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSP LKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQP LSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTV DKSTGKPTLYNVSLVMSDTAGTCY" BASE COUNT 419 a 618 c 561 g 380 t ORIGIN 1 ggagctctga gagaggagcc cagccctggg attttcaggt gttttcattt ggtgatcagg 61 actgaacaga gagaactcac catggagttt gggctgagct ggctttttct tgtggctatt 121 ttaaaaggtg tccagtgtga ggtgcagctg ttggagtctg ggggaggctc ggtacagcct 181 ggggggtccc tgagactgtc ctgtgtaccc tctggattcg cctttagcta ctatgccatg 241 agctgggtcc gccaggctcc agggaagggg ctggagtggg tctcaactat tagtggtggt 301 ggtagcacag agtacgcaga ctccgtgaag ggccggttca ccatctccag agacaattcc 361 aagaacacgc tgtatctgca aatgaacagc ctgagagccg aggacacggc cgtatattac 421 tgtgcgaggg gcggtggtcc ctactggtac ttcgatctct ggggccgtgg caccctggtc 481 actgtctcct cagggagtgc atccgcccca acccttttcc ccctcgtctc ctgtgagaat 541 tccccgtcgg atacgagcag cgtggccgtt ggctgcctcg cacaggactt ccttcccgac 601 tccatcactt tctcctggaa atacaagaac aactctgaca tcagcagcac ccggggcttc 661 ccatcagtcc tgagaggggg caagtacgca gccacctcac aggtgctgct gccttccaag 721 gacgtcatgc agggcacaga cgaacacgtg gtgtgcaaag tccagcaccc caacggcaac 781 aaagaaaaga acgtgcctct tccagtgatt gccgagctgc ctcccaaagt gagcgtcttc 841 gtcccacccc gcgacggctt cttcggcaac ccccgcaagt ccaagctcat ctgccaggcc 901 acgggtttca gtccccggca gattcaggtg tcctggctgc gcgaggggaa gcaggtgggg 961 tctggcgtca ccacggacca ggtgcaggct gaggccaaag agtctgggcc cacgacctac 1021 aaggtgacca gcacactgac catcaaagag agcgactggc tcagccagag catgttcacc 1081 tgccgcgtgg atcacagggg cctgaccttc cagcagaatg cgtcctccat gtgtgtcccc 1141 gatcaagaca cagccatccg ggtcttcgcc atccccccat cctttgccag catcttcctc 1201 accaagtcca ccaagttgac ctgcctggtc acagacctga ccacctatga cagcgtgacc 1261 atctcctgga cccgccagaa tggcgaagct gtgaaaaccc acaccaacat ctccgagagc 1321 caccccaatg ccactttcag cgccgtgggt gaggccagca tctgcgagga tgactggaat 1381 tccggggaga ggttcacgtg caccgtgacc cacacagacc tgccctcgcc actgaagcag 1441 accatctccc ggcccaaggg ggtggccctg cacaggcccg atgtctactt gctgccacca 1501 gcccgggagc agctgaacct gcgggagtcg gccaccatca cgtgcctggt gacgggcttc 1561 tctcccgcgg acgtcttcgt gcagtggatg cagagggggc agcccttgtc cccggagaag 1621 tatgtgacca gcgccccaat gcctgagccc caggccccag gccggtactt cgcccacagc 1681 atcctgaccg tgtccgaaga ggaatggaac acgggggaga cctacacctg cgtggtggcc 1741 catgaggccc tgcccaacag ggtcaccgag aggaccgtgg acaagtccac cggtaaaccc 1801 accctgtaca acgtgtccct ggtcatgtcc gacacagctg gcacctgcta ctgaccctgc 1861 tggcctgccc acaggctcgg ggcggctggc cgctctgtgt gtgcatgcaa actaaccgtg 1921 tcaacggggt gagatgttgc atcttataaa attagaaata aaaagatcca ttcaaaag // LOCUS F3005_3N8_lambda_2-23 887 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3N8 monoclonal IgM antibody lambda chain mRNA, complete cds. ACCESSION F3005_3N8_lambda_2-23 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 887) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 887) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..887 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 41..748 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWALLLLTLLTQDTGSWAQSALTQPASESGSPGQSITISCTGT SSDVGSYNLVSWYQQHPGKAPKLMIYEGSKRPSGVSNRFSGSKSGNTASLTISGLQAE DEADYYCCSYAGSTTHVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLI SDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ VTHEGSTVEKTVAPTECS" BASE COUNT 200 a 287 c 228 g 172 t ORIGIN 1 ggggggtcac aagaggcagc gctctcggga cgtctccacc atggcctggg ctctgctgct 61 cctcactctc ctcactcagg acacagggtc ctgggcccag tctgccctga ctcagcctgc 121 ctccgagtct gggtctcctg gacagtcgat caccatctcc tgcactggaa ccagcagtga 181 tgttgggagt tataaccttg tctcctggta ccaacagcac ccaggcaaag cccccaaact 241 catgatttat gagggcagta agcggccctc aggggtttct aatcgcttct ctggctccaa 301 gtctggcaac acggcctccc tgacaatctc tgggctccag gctgaggacg aggctgatta 361 ttactgctgc tcatatgcag gaagtaccac tcatgtggta ttcggtggag ggaccaagct 421 gaccgtcctg ggtcagccca aggctgcccc ctcggtcact ctgttcccgc cctcctctga 481 ggagcttcaa gccaacaagg ccacactggt gtgtctcata agtgacttct acccgggagc 541 cgtgacagtg gcctggaagg cagatagcag ccccgtcaag gcgggagtgg agaccaccac 601 accctccaaa caaagcaaca acaagtacgc ggccagcagc tacctgagcc tgacgcctga 661 gcagtggaag tcccacagaa gctacagctg ccaggtcacg catgaaggga gcaccgtgga 721 gaagacagtg gcccctacag aatgttcata ggttctcaac cctcaccccc accacgggag 781 actagagctg caggatccca ggggaggggt ctctcctccc accccaaggc atcaagccct 841 tctccctgca ctcaataaac cctcaataaa tattctcatt gtcaatc // LOCUS F3007_1K11_gamma1_4-59 1606 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1K11 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3007_1K11_gamma1_4-59 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1606) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1606) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1606 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 38..1468 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MKHLWFFLLLVTAPRWVLSQVQLQESGPGLIKPSETLSLTCTVS GGSISYYYWSWIRQPPGKGLEWIGNVYFSGTTNYKSSLKSRVTISVDTSRNQFSLEVS SVTAADTAVYYCARVTRYCTKGVCQTDYFDGMDVWGQGTTVTVSSASTKGPSVFPLAP SSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTV PSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPK PKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 359 a 526 c 427 g 294 t ORIGIN 1 ggactttctg agagtcctgg acctcctgtg caagaacatg aaacatctgt ggttcttcct 61 tctcctggtg acagctccca gatgggtcct gtcccaagtg cagctgcagg agtcgggccc 121 aggactgata aagccttcgg agaccctgtc cctcacttgc actgtctctg gtggctccat 181 tagctattac tattggagct ggatccggca gcccccaggg aagggactgg agtggattgg 241 aaatgtctat tttagtggga ccaccaacta caaatcctcc ctcaagagtc gagtcaccat 301 ttcagtggac acgtccagaa accagttctc cctggaagtg agctctgtga ccgctgcgga 361 cacggccgtc tattactgtg cgagagtgac ccgatattgt actaaaggtg tctgtcaaac 421 ggactacttc gacggtatgg acgtctgggg ccaggggacc acggtcaccg tctcctcagc 481 ctccaccaag ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg 541 cacagcggcc ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg 601 gaactcaggc gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg 661 actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta 721 catctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa 781 atcttgtgac aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc 841 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga 901 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta 961 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag 1021 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga 1081 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa 1141 agccaaaggg cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct 1201 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc 1261 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct 1321 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca 1381 gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca 1441 gaagagcctc tccctgtctc cgggtaaatg agtgcgacgg ccggcaagcc cccgctcccc 1501 gggctctcgc ggtcgcacga ggatgcttgg cacgtacccc ctgtacatac ttcccgggcg 1561 cccagcatgg aaataaagca cccagcgctg ccctgggccc ctgcca // LOCUS F3007_1K11_lambda1_1-40 968 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1K11 monoclonal IgG antibody lambda chain mRNA, complete cds. ACCESSION F3007_1K11_lambda1_1-40 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 968) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 968) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..968 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 55..759 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWSPLLLTLLAHCTGSWAQSVLTQPPSVSGAPGQRVTISCTGG SSNIGAGYDVHWYQQIPGTAPRLLIYDNTNRPSGVPDRFSGSKSGTSASLAITGLLAE DEADYYCQSYDGSLGGVFGTGTKVTVLRQPKANPTVTLFPPSSEELQANKATLVCLIS DFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQV THEGSTVEKTVAPTECS" BASE COUNT 209 a 318 c 246 g 195 t ORIGIN 1 gaggctctgc ttcagctgtg ggcacaagag gcagcactca ggacaatctc cagcatggcc 61 tggtctcctc tcctcctcac tctcctcgct cactgcacag ggtcctgggc ccagtctgtg 121 ctgacgcagc cgccctcagt ttctggggcc ccagggcaga gggtcaccat ctcctgcact 181 gggggaagtt ccaacatcgg ggcgggttat gatgtacact ggtaccagca gattccagga 241 acagccccca gactcctcat ctatgataac accaatcggc cctcaggggt ccctgaccga 301 ttctctggct ccaagtctgg cacctccgcc tccctggcca tcactggtct cctggctgag 361 gatgaggctg attactactg ccagtcctat gacggcagcc tgggaggggt cttcggaact 421 gggaccaagg tcaccgtcct tcgtcagccc aaggccaacc ccactgtcac tctgttcccg 481 ccctcctctg aggagctcca agccaacaag gccacactag tgtgtctgat cagtgacttc 541 tacccgggag ctgtgacagt ggcctggaag gcagatggca gccccgtcaa ggcgggagtg 601 gagaccacca aaccctccaa acagagcaac aacaagtacg cggccagcag ctacctgagc 661 ctgacgcccg agcagtggaa gtcccacaga agctacagct gccaggtcac gcatgaaggg 721 agcaccgtgg agaagacagt ggcccctaca gaatgttcat aggttcccaa ctctaacccc 781 acccacggga gcctggagct gcaggatccc aggggagggg tctctctccc catcccaagt 841 catccagccc ttctccctgc actcatgaaa ccccaataaa tatcctcatt gacaaccaga 901 aatcttgttt tatctcattt tttttctcac ataaattgct agcctccccg gggttctcag 961 tgtggggt // LOCUS F2978_1M18_gamma1_1-2 1622 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1M18 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F2978_1M18_gamma1_1-2 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1622) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1622) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1622 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 64..1488 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDWTWRILFLVAAATGAHSQVQLVQSGAEVKKPGASVKVSCKAS GYTFTGYYMHWVRQAPGQGLEWMGWINPISGGTNYAQKFQGRVTMTRDTSISTAYMEL SRLRSDDTAVYYCARDERSGDFWTAYYYHFDYWGQGTLVTVSSASTKGPSVFPLAPSS KSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPS SSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 377 a 526 c 437 g 282 t ORIGIN 1 ggagcatcac ccagcaacca catctgtcct ctagagaatc ccctgagagc tccgttcctc 61 accatggact ggacctggag gatcctcttc ttggtggcag cagccacagg agcccactcc 121 caggtgcagc tggtgcagtc tggggctgag gtgaagaagc ctggggcctc agtgaaggtc 181 tcctgtaagg cttctggata caccttcacc ggctactata tgcactgggt gcgacaggcc 241 cctggacaag ggcttgagtg gatgggatgg atcaacccta tcagtggtgg cacaaactat 301 gcacagaagt ttcagggcag ggtcaccatg accagggaca cgtccatcag cacagcctac 361 atggagctca gcaggttgag atctgacgac acggccgtgt attactgtgc gagagacgaa 421 aggtcaggcg atttttggac tgcttattat taccactttg actactgggg ccagggaacc 481 ctggtcaccg tctcctcagc ctccaccaag ggcccatcgg tcttccccct ggcaccctcc 541 tccaagagca cctctggggg cacagcggcc ctgggctgcc tggtcaagga ctacttcccc 601 gaaccggtga cggtgtcgtg gaactcaggc gccctgacca gcggcgtgca caccttcccg 661 gctgtcctac agtcctcagg actctactcc ctcagcagcg tggtgaccgt gccctccagc 721 agcttgggca cccagaccta catctgcaac gtgaatcaca agcccagcaa caccaaggtg 781 gacaagagag ttgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccagca 841 cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc 901 atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct 961 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg 1021 cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag 1081 gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc 1141 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt gtacaccctg 1201 cccccatccc gggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc 1261 ttctatccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac 1321 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caagctcacc 1381 gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat gcatgaggct 1441 ctgcacaacc actacacgca gaagagcctc tccctgtccc cgggtaaatg agtgcgacgg 1501 ccggcaagcc cccgctcccc gggctctcgc ggtcgcacga ggatgcttgg cacgtacccc 1561 gtctacatac ttcccaggca cccagcatgg aaataaagca cccaccactg ccctggaaaa 1621 aa // LOCUS F2978_1M18_kappa_1-12 936 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1M18 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F2978_1M18_kappa_1-12 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 936) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 936) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..936 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 30..737 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MDMRVPAQLLGLLLLWFPGSRCDIQMTQSPSSVSASVGDRVTIT CRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQP EDFATYYCQQANSFPLTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNN FYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEV THQGLSSPVTKSFNRGEC" BASE COUNT 225 a 279 c 225 g 207 t ORIGIN 1 ggaggagtca gacccagtca ggacacagca tggacatgag ggtccccgct cagctcctgg 61 ggctcctgct gctctggttc ccaggttcca gatgcgacat ccagatgacc cagtctccat 121 cttccgtgtc tgcatctgta ggagacagag tcaccatcac ttgtcgggcg agtcagggta 181 ttagcagctg gttagcctgg tatcagcaga aaccagggaa agcccctaag ctcctgatct 241 atgctgcatc cagtttgcaa agtggggtcc catcaaggtt cagcggcagt ggatctggga 301 cagatttcac tctcaccatc agcagcctgc agcctgaaga ttttgcaact tactattgtc 361 aacaggctaa cagtttcccc ctcaccttcg gccaagggac acgactggag attaaacgaa 421 ctgtggctgc accatctgtc ttcatcttcc cgccatctga tgagcagttg aaatctggaa 481 ctgcctctgt tgtgtgcctg ctgaataact tctatcccag agaggccaaa gtacagtgga 541 aggtggataa cgccctccaa tcgggtaact cccaggagag tgtcacagag caggacagca 601 aggacagcac ctacagcctc agcagcaccc tgacgctgag caaagcagac tacgagaaac 661 acaaagtcta cgcctgcgaa gtcacccatc agggcctgag ctcgcccgtc acaaagagct 721 tcaacagggg agagtgttag agggagaagt gcccccacct gctcctcagt tccagcctga 781 ccccctccca tcctttggcc tctgaccctt tttccacagg ggacctaccc ctattgcggt 841 cctccagctc atctttcacc tcacccccct cctcctcctt ggctttaatt atgctaatgt 901 tggaggagaa tgaataaata aagtgaatct ttgcac // LOCUS F3005_3H9_gamma1_2-5 1511 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3H9 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3005_3H9_gamma1_2-5 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1511) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1511) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1511 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 23..1432 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDTLCSTLLLLTIPSWVLSQITLKESGPTLVKPTQTLTLTCTFS GFSLTTGGVGVGWIRQPPGKALEWLALIYWNDDKRYSPSLKSRLTITKDTSKNQVVLT MTNMDPVDTATYFCVHGYSWYYYGMDVWGQGTKVTVSSASTKGPSVFPLAPSSKSTSG GTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGT QTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSV MHEALHNHYTQKSLSLSPGK" BASE COUNT 343 a 504 c 391 g 273 t ORIGIN 1 ggagtgactc ctgtgcccca ccatggacac actttgctcc acgctcctgc tgctgaccat 61 cccttcatgg gtcttgtccc agatcacctt gaaggagtct ggtcctacgc tggtgaaacc 121 cacacagacc ctcacgctga cctgcacctt ctctgggttc tcactcacca ctggtggagt 181 gggtgtgggc tggatccgtc agcccccagg aaaggccctg gagtggcttg cactcattta 241 ttggaatgat gataagcgct acagcccatc tctgaagagc aggctcacca tcaccaagga 301 cacctccaaa aaccaggtgg tccttacaat gaccaacatg gaccctgtgg acacagccac 361 atatttctgt gtacatggat atagttggta ctattacggt atggacgtct ggggccaagg 421 gaccaaggtc accgtctcct cagcctccac caagggccca tcggtcttcc ccctggcacc 481 ctcctccaag agcacctctg ggggcacagc ggccctgggc tgcctggtca aggactactt 541 ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt 601 cccggctgtc ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc 661 cagcagcttg ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa 721 ggtggacaag aaagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc 781 agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac 841 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga 901 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa 961 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca 1021 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc 1081 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac 1141 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa 1201 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa 1261 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct 1321 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga 1381 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aatgagtgcg 1441 acggccggca agcccccgct ccccgggctc tcgcggtcgc acgaggatgc ttggcacgta 1501 ccccctgtac a // LOCUS F3005_3H9_lambda_2-14 826 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 3H9 monoclonal IgG antibody lambda chain mRNA, complete cds. ACCESSION F3005_3H9_lambda_2-14 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 826) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 826) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..826 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 44..751 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWALLFLTLLTQGTGSWAQSALTQPASVSGSPGQSITISCTGT SSDIGDYNSVSWYQQHPGKAPKLMIFDVINRPSGVSNRFSGSKSGNTASLTISGLQAD DEADYYCSSYTASSTLGVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLI SDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ VTHEGSTVEKTVAPTECS" BASE COUNT 178 a 271 c 217 g 160 t ORIGIN 1 gggcgggggt ctcaggaggc agcgctctcg ggacgtctcc accatggcct gggctctgct 61 attcctcacc ctcctcactc agggcacagg gtcctgggcc cagtctgccc tgactcagcc 121 tgcctccgtg tctgggtctc ctggacagtc gatcaccatc tcctgcactg gaaccagcag 181 tgacattggt gattataact ctgtctcctg gtaccaacaa cacccaggca aagcccccaa 241 actcatgatt tttgatgtca ttaatcggcc ctcaggggtt tctaatcgct tctctggctc 301 caagtctggc aacacggcct ccctgaccat ctctgggctc caggctgatg acgaggctga 361 ttattactgc agctcatata cagccagcag cactctcggg gtattcggcg gagggaccaa 421 gctgaccgtc ctaggtcagc ccaaggctgc cccctcggtc actctgttcc cgccctcctc 481 tgaggagctt caagccaaca aggccacact ggtgtgtctc ataagtgact tctacccggg 541 agccgtgaca gtggcctgga aggcagatag cagccccgtc aaggcgggag tggagaccac 601 cacaccctcc aaacaaagca acaacaagta cgcggccagc agctatctga gcctgacgcc 661 tgagcagtgg aagtcccaca gaagctacag ctgccaggtc acgcatgaag ggagcaccgt 721 ggagaagaca gtggccccta cagaatgttc ataggttcta aaccctcacc ccccccacgg 781 gagactagag ctgcaggatc ccaggggagg ggtctctcct cccacc // LOCUS F2978_2O17_gamma1_3-30 1636 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2O17 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F2978_2O17_gamma1_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1636) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1636) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1636 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 84..1493 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLNWVFLVALLRGVQCQVQLVESGGGVVQSGRSLRLSCAAS GFTFSSYGMHWVRQAPGKGLEWVAVISYDGSNKYYADSVRGRFTISRDNSKNTLYLQM NSLRAEDTAVYYCARPYSGSYWSAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSG GTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGT QTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSV MHEALHNHYTQKSLSLSPGK" BASE COUNT 371 a 506 c 459 g 300 t ORIGIN 1 ggggagctct gggagaggag cccagcacta gaagtcggcg gtgtttccat tcggtgatca 61 gcactgaaca cagaggactc accatggagt ttgggctgaa ctgggttttc ctcgttgctc 121 ttttaagagg tgtccagtgt caggtgcagc tggtggagtc tgggggaggc gtggtccagt 181 ctgggaggtc cctgagactc tcctgtgcag cctctggatt caccttcagt agctatggca 241 tgcactgggt ccgccaggct ccaggcaagg ggctggagtg ggtggcagtt atatcatatg 301 atggaagtaa taaatactat gcagactccg tgaggggccg attcaccatc tccagagaca 361 attccaagaa cacgctgtat ctgcaaatga acagcctgag agctgaggac acggctgtgt 421 attactgtgc gagaccgtat agtgggagct actggagtgc ttttgatatc tggggccaag 481 ggacaatggt caccgtctct tcagcctcca ccaagggccc atcggtcttc cccctggcac 541 cctcctccaa gagcacctct gggggcacag cggccctggg ctgcctggtc aaggactact 601 tccccgaacc ggtgacggtg tcgtggaact caggcgccct gaccagcggc gtgcacacct 661 tcccggctgt cctacagtcc tcaggactct actccctcag cagcgtggtg accgtgccct 721 ccagcagctt gggcacccag acctacatct gcaacgtgaa tcacaagccc agcaacacca 781 aggtggacaa gaaagttgag cccaaatctt gtgacaaaac tcacacatgc ccaccgtgcc 841 cagcacctga actcctgggg ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca 901 ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg agccacgaag 961 accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat gccaagacaa 1021 agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc accgtcctgc 1081 accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa gccctcccag 1141 cccccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca caggtgtaca 1201 ccctgccccc atcccgggat gagctgacca agaaccaggt cagcctgacc tgcctggtca 1261 aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag ccggagaaca 1321 actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc tacagcaagc 1381 tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg 1441 aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt aaatgagtgc 1501 gacggccggc aagcccccgc tccccgggct ctcgcggtcg cacgaggatg cttggcacgt 1561 accccctgta catacttccc gggcgcccag catggaaata aagcacccag cgctgccctg 1621 ggcccctgcg aaaaaa // LOCUS F2978_2O17_lambda_1-51 894 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2O17 monoclonal IgG antibody lambda chain mRNA, complete cds. ACCESSION F2978_2O17_lambda_1-51 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 894) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 894) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..894 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 55..759 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MTCSPLLLTLLIHCTGSWAQSVLTQPPSVSAAPGQRVTISCSGG YSNIETNYVSWYQRLPGTAPKLLIYDNDKRPSGIPDRFSGSRSGTSATLGITGLQTGD EADYYCAAWDTSLSGVVFGGGTKLTVLRQPKAAPSVTLFPPSSEELQANKATLVCLIS DFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQV THEGSTVEKTVAPTECS" BASE COUNT 204 a 294 c 225 g 171 t ORIGIN 1 gaagctctgc ttcagctgtg agcgcagaag gcaggactcg ggacaatctt catcatgacc 61 tgctcccctc tcctcctcac ccttctcatt cactgcacag ggtcctgggc ccagtctgtg 121 ttgacgcagc cgccctcagt gtctgcggcc ccgggacaga gggtcaccat ctcctgctct 181 ggaggctact ccaacattga gactaattat gtatcctggt accagcgact cccaggaaca 241 gcccccaaac tcctcattta tgacaatgat aagcgaccct cagggattcc tgaccgattc 301 tctggctcca gatctggcac gtcagccacc ctgggcatca ccggactcca gactggggac 361 gaggccgatt attactgcgc ggcatgggat accagcctga gtggtgtggt attcggcgga 421 gggaccaagc tgaccgtcct acgtcagccc aaggctgccc cctcggtcac tctgttcccg 481 ccctcctctg aggagcttca agccaacaag gccacactgg tgtgtctcat aagtgacttc 541 tacccgggag ccgtgacagt ggcctggaag gcagatagca gccccgtcaa ggcgggagtg 601 gagaccacca caccctccaa acaaagcaac aacaagtacg cggccagcag ctatctgagc 661 ctgacgcctg agcagtggaa gtcccacaga agctacagct gccaggtcac gcatgaaggg 721 agcaccgtgg agaagacagt ggcccctaca gaatgttcat aggttctaaa ccctcacccc 781 ccccacggga gactagagct gcaggatccc aggggagggg tctctcctcc caccccaagg 841 catcaagccc ttctccctgc actcaataaa ccctcaataa atattctcat tgtc // LOCUS F2978_2C20_alpha_1-2 1607 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2C20 monoclonal IgA antibody heavy chain mRNA, complete cds. ACCESSION F2978_2C20_alpha_1-2 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1607) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1607) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1607 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 22..1512 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDWTWRILFLVAAATGAHSQVQLVQSGAEVKKPGASVKVSCKAS GYTFTGYYMHWVRQAPGQGLEWMGWINPISGGTNYAQKFQGRVTMTRDTSISTAYMEL SRLRSDDTAVYYCARVSYFYDNSGYPGAFDIWGQGTMVTVSSASPTSPKVFPLSLCST QPDGNVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPA TQCLAGKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRP ALEDLLLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPERDLCGCYSVSSVLP GCAEPWNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTL TCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWK KGDTFSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY" BASE COUNT 361 a 529 c 433 g 284 t ORIGIN 1 ctgagagctc cgttcctcac catggactgg acctggagga tcctcttctt ggtggcagca 61 gccacaggag cccactccca ggtgcagctg gtgcagtctg gggctgaggt gaagaagcct 121 ggggcctcag tgaaggtctc ctgcaaggct tctggataca ccttcaccgg ctactatatg 181 cactgggtgc gacaggcccc tggacaaggg cttgagtgga tgggatggat caaccctatc 241 agtggtggca caaactatgc acagaagttt cagggcaggg tcaccatgac cagggacacg 301 tccatcagca cagcctacat ggagctgagc aggctgagat ctgacgacac ggccgtgtat 361 tactgtgcga gagtctcgta tttctatgat aatagtggtt accccggtgc ttttgatatc 421 tggggccaag ggacaatggt caccgtctct tcagcatccc cgaccagccc caaggtcttc 481 ccgctgagcc tctgcagcac ccagccagat gggaacgtgg tcatcgcctg cctggtccag 541 ggcttcttcc cccaggagcc actcagtgtg acctggagcg aaagcggaca gggcgtgacc 601 gccagaaact tcccacccag ccaggatgcc tccggggacc tgtacaccac gagcagccag 661 ctgaccctgc cggccacaca gtgcctagcc ggcaagtccg tgacatgcca cgtgaagcac 721 tacacgaatc ccagccagga tgtgactgtg ccctgcccag ttccctcaac tccacctacc 781 ccatctccct caactccacc taccccatct ccctcatgct gccacccccg actgtcactg 841 caccgaccgg ccctcgagga cctgctctta ggttcagaag cgaacctcac gtgcacactg 901 accggcctga gagatgcctc aggtgtcacc ttcacctgga cgccctcaag tgggaagagc 961 gctgttcaag gaccacctga gcgtgacctc tgtggctgct acagcgtgtc cagtgtcctg 1021 ccgggctgtg ccgagccatg gaaccatggg aagaccttca cttgcactgc tgcctacccc 1081 gagtccaaga ccccgctaac cgccaccctc tcaaaatccg gaaacacatt ccggcccgag 1141 gtccacctgc tgccgccgcc gtcggaggag ctggccctga acgagctggt gacgctgacg 1201 tgcctggcac gcggcttcag ccccaaggac gtgctggttc gctggctgca ggggtcacag 1261 gagctgcccc gcgagaagta cctgacttgg gcatcccggc aggagcccag ccagggcacc 1321 accaccttcg ctgtgaccag catactgcgc gtggcagccg aggactggaa gaagggggac 1381 accttctcct gcatggtggg ccacgaggcc ctgccgctgg ccttcacaca gaagaccatc 1441 gaccgcttgg cgggtaaacc cacccatgtc aatgtgtctg ttgtcatggc ggaggtggac 1501 ggcacctgct actgagccgc ccgcctgtcc ccacccctga ataaactcca tgctccccca 1561 aacaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa // LOCUS F2978_2C20_lambda_2-14 876 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2C20 monoclonal IgA antibody lambda chain mRNA, complete cds. ACCESSION F2978_2C20_lambda_2-14 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 876) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 876) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..876 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 44..748 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWALLLLTLLTQGTGSWAQSALTQPASVSGSPGQSITISCTGT SSDVGSYNFVSWYQQHPGKAPKLMIYEVSNRPSGVSNRFSGSKSGDTASLTISGLQAG DEADYYCSSYTSSSTWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLIS DFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQV THEGSTVEKTVAPTECS" BASE COUNT 194 a 289 c 224 g 169 t ORIGIN 1 gggctggggt ctcaggaggc agcgctctca ggacatctcc accatggcct gggctctgct 61 gctcctcacc ctcctcactc agggcacagg gtcctgggcc cagtctgccc tgactcagcc 121 tgcctccgtg tctgggtctc ctggacagtc gatcaccatc tcctgcactg gaaccagcag 181 tgacgttggt agttataact ttgtctcctg gtaccaacag cacccaggca aagcccccaa 241 actcatgatt tatgaggtca gtaatcggcc ctcaggggtt tctaatcgct tctctggctc 301 caagtctggc gacacggcct ccctgaccat ctctgggctc caggctgggg acgaggctga 361 ttattactgc agctcatata caagcagcag cacttgggtg ttcggcggag ggaccaagct 421 gaccgtccta ggtcagccca aggctgcccc ctcggtcact ctgttcccac cctcctctga 481 ggagcttcaa gccaacaagg ccacactggt gtgtctcata agtgacttct acccgggagc 541 cgtgacagtg gcctggaagg cagatagcag ccccgtcaag gcgggagtgg agaccaccac 601 accctccaaa caaagcaaca acaagtacgc ggccagcagc tacctgagcc tgacgcctga 661 gcagtggaag tcccacaaaa gctacagctg ccaggtcacg catgaaggga gcaccgtgga 721 gaagacagtg gcccctacag aatgttcata ggttctcatc cctcaccccc caccacggga 781 gactagagct gcaggatccc aggggagggg tctctcctcc caccccaagg catcaagccc 841 ttctccctgc actcaataaa ccctcaataa atattc // LOCUS F2978_1I12_gamma1_3-30 1446 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1I12 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F2978_1I12_gamma1_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1446) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1446) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1446 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1443 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAAS GFTFSRNAMHWVRQAPGKGLEWVAVISYHGSNQYYADSVKGRFTISRDNSKNTLYLQM NSLRAEDTAVYYCAKDQVEVEPILMMFGGLTTASVGDVWGQGTPVTVSSASTKGPSVF PLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSS VVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTY RVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDEL TKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSR WQQGNVFSCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 332 a 444 c 400 g 270 t ORIGIN 1 atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgt ccagtgtcag 61 gtgcagctgg tggagtctgg gggaggcgtg gtccagcctg ggaggtccct gagactctcc 121 tgtgcagcct ctggattcac cttcagtagg aatgctatgc actgggtccg ccaggctcca 181 ggcaaggggc tggagtgggt ggcagttata tcatatcatg gaagtaatca atactatgca 241 gactccgtga agggccgatt caccatctcc agagacaatt ccaagaacac gctgtatctg 301 caaatgaaca gcctgagagc tgaagacacg gctgtgtatt actgtgcaaa agatcaagtg 361 gaagttgagc ccattttgat gatgtttggg ggacttacga ccgcatccgt gggggacgtc 421 tggggccaag ggaccccggt caccgtctcc tcagcctcca ccaagggccc atcggtcttc 481 cccctggcac cctcctccaa gagcacctct gggggcacag cggccctggg ctgcctggtc 541 aaggactact tccccgaacc ggtgacggtg tcgtggaact caggcgccct gaccagcggc 601 gtgcacacct tcccggctgt cctacagtcc tcaggactct actccctcag cagcgtggtg 661 accgtgccct ccagcagctt gggcacccag acctacatct gcaacgtgaa tcacaagccc 721 agcaacacca aggtggacaa gaaagttgag cccaaatctt gtgacaaaac tcacacatgc 781 ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt ccccccaaaa 841 cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg 901 agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat 961 gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc 1021 accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa 1081 gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca 1141 caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt cagcctgacc 1201 tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag 1261 ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc 1321 tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc 1381 gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt 1441 aaatga // LOCUS F2978_1I12_kappa_3-15 938 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1I12 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F2978_1I12_kappa_3-15 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 938) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 938) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..938 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 34..738 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MEAPAQLLFLLLLWLPETTGEIVMTQSPATLSVSPGERATLSCR ASQSVSGNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQSED FAVYYCQQYNNWPPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNF YPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC" BASE COUNT 222 a 287 c 228 g 201 t ORIGIN 1 gaggaactgc tcagttagga cccagacgga accatggaag ccccagcgca gcttctcttc 61 ctcctgctac tctggctccc agagaccact ggagaaatag tgatgacgca gtctccagcc 121 accctgtctg tgtctccagg ggaaagagcc accctctcct gcagggccag tcagagtgtt 181 agcggcaact tagcctggta ccagcagaaa cctggccagg ctcccaggct cctcatctat 241 ggtgcatcca ccagggccac tggtatccca gccaggttca gtggcagtgg gtctgggaca 301 gagttcactc tcaccatcag cagcctgcag tctgaagatt ttgcagttta ttactgtcag 361 cagtataata actggcctcc gctcactttc ggcgggggga ccaaggtgga gatcaaacga 421 actgtggctg caccatctgt cttcatcttc ccgccatctg atgagcagtt gaaatctgga 481 actgcctctg ttgtgtgcct gctgaataac ttctatccca gagaggccaa agtacagtgg 541 aaggtggata acgccctcca atcgggtaac tcccaggaga gtgtcacaga gcaggacagc 601 aaggacagca cctacagcct cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa 661 cacaaagtct acgcctgcga agtcacccat cagggcctga gctcgcccgt cacaaagagc 721 ttcaacaggg gagagtgtta gagggagaag tgcccccacc tgctcctcag ttccagcctg 781 accccctccc atcctttggc ctctgaccct ttttccacag gggacctacc cctattgcgg 841 tcctccagct catctttcac ctcacccccc tcctcctcct tggctttaat tatgctaatg 901 ttggaggaga atgaataaat aaagtgaatc tttgcacc // LOCUS F3015_1J12_gamma1_3-30 1422 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1J12 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3015_1J12_gamma1_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1422) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1422) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1422 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1419 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAAS GFTFSFFGMHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQM NSLRAEDTAVYYCAKAKDIVVLPALNWFDPWGQGTLVTVSSASTKGPSVFPLAPSSKS TSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 324 a 442 c 390 g 266 t ORIGIN 1 atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgt ccagtgtcag 61 gtgcagctgg tggagtctgg gggaggcgtg gtccagcctg ggaggtccct gagactctcc 121 tgtgcagcct ctggattcac cttcagtttc tttggcatgc actgggtccg ccaggctcca 181 ggcaaggggc tggagtgggt ggcagttatt tcgtatgatg gaagtaataa atactatgca 241 gactccgtga agggccgatt caccatctcc agagacaatt ccaagaacac gctgtatctg 301 caaatgaaca gcctgagagc tgaggacacg gctgtctatt actgtgcgaa agcgaaggat 361 attgtagtac taccagcact caactggttc gacccctggg gccagggaac cctggtcacc 421 gtctcctcag cctccaccaa gggcccatcg gtcttccccc tggcaccctc ctccaagagc 481 acctctgggg gcacagcggc cctgggctgc ctggtcaagg actacttccc cgaaccggtg 541 acggtgtcgt ggaactcagg cgccctgacc agcggcgtgc acaccttccc ggctgtccta 601 cagtcctcag gactctactc cctcagcagc gtggtgaccg tgccctccag cagcttgggc 661 acccagacct acatctgcaa cgtgaatcac aagcccagca acaccaaggt ggacaagaga 721 gttgagccca aatcttgtga caaaactcac acatgcccac cgtgcccagc acctgaactc 781 ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc 841 cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag 901 ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag 961 cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg 1021 aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa 1081 accatctcca aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc 1141 cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc 1201 agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg 1261 cctcccgtgc tggactccga cggctccttc ttcctctata gcaagctcac cgtggacaag 1321 agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc tctgcacaac 1381 cactacacgc agaagagcct ctccctgtcc ccgggtaaat ga // LOCUS F3015_1J12_lambda_2-14 813 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1J12 monoclonal IgG antibody lambda chain mRNA, complete cds. ACCESSION F3015_1J12_lambda_2-14 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 813) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 813) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..813 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 39..746 /codon_start=1 /product="immunoglobulin lambda chain" /translation="MAWALLLLTLLTQGTGSWAQSALTQPASVSGSPGQSITISCTGT SSDVGGYNYVSWYQQHPGKAPKLMIYEVSNRPSGVSNRFSGSKSGNTASLTISGLQAE DEADYYCSSYTSSSTPFVFGTGTKVTVLGQPKANPTVTLFPPSSEELQANKATLVCLI SDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ VTHEGSTVEKTVAPTECS" BASE COUNT 180 a 266 c 213 g 154 t ORIGIN 1 ggggtctcag gaggcagcgc tctcaggaca tctccaccat ggcctgggct ctgctgctcc 61 tcaccctcct cactcagggc acagggtcct gggcccagtc tgccctgact cagcctgcct 121 ccgtgtctgg gtctcctgga cagtcgatca ccatctcctg cactggaacc agcagtgacg 181 ttggtggtta taactatgtc tcctggtacc aacaacaccc aggcaaagcc cccaaactca 241 tgatttatga ggtcagtaat cggccctcag gggtttctaa tcgcttctct ggctccaagt 301 ctggcaacac ggcctccctg accatctctg ggctccaggc tgaggacgag gctgattatt 361 actgcagctc atatacaagc agcagcaccc cttttgtctt cggaactggg accaaggtca 421 ccgtcctagg tcagcccaag gccaacccca ctgtcactct gttcccgccc tcctctgagg 481 agctccaagc caacaaggcc acactagtgt gtctgatcag tgacttctac ccgggagctg 541 tgacagtggc ctggaaggca gatggcagcc ccgtcaaggc gggagtggag accaccaaac 601 cctccaaaca gagcaacaac aagtacgcgg ccagcagcta cctgagcctg acgcccgagc 661 agtggaagtc ccacagaagc tacagctgcc aggtcacgca tgaagggagc accgtggaga 721 agacagtggc ccctacagaa tgttcatagg ttcccaactc taaccccacc cacgggagcc 781 tggagctgca ggatcccagg ggaggggtct ctc // LOCUS F2978_2J12_alpha_3-30 1479 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2J12 monoclonal IgA antibody heavy chain mRNA, complete cds. ACCESSION F2978_2J12_alpha_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1479) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1479) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1479 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1476 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWVFLVALLRGVQCQVQLAESGGGVVQPGRSLRLSCAAS GFTFNSYAVHWVRQAPGKGLEWVAVISYDGSNKYYADSVKGRFTISRDNSKNTLYLQM NSLRAEDTAVYYCARDRSGSYTYFDYWGQGTLVTVSSASPTSPKVFPLSLCSTQPDGN VVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYATSSQLTLPATQCLA GKSVTCHVKHYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPRLSLHRPALEDL LLGSEANLTCTLTGLRDASGVTFTWTPSSGKSAVQGPPDRDLCGCYSVSSVLSGCAEP WNHGKTFTCTAAYPESKTPLTATLSKSGNTFRPEVHLLPPPSEELALNELVTLTCLAR GFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTTTFAVTSILRVAAEDWKKGDTF SCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEVDGTCY" BASE COUNT 296 a 485 c 420 g 278 t ORIGIN 1 atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgt ccagtgtcag 61 gtgcagctgg cggagtctgg gggaggcgtg gtccagcctg ggaggtccct gagactctcc 121 tgtgcagcct ctggattcac cttcaatagc tatgctgtgc actgggtccg ccaggctcca 181 ggcaaggggc tggagtgggt ggcagttata tcatatgatg gaagcaataa atactacgca 241 gactccgtga agggccgatt caccatctcc agagacaatt ccaagaacac gctgtatctg 301 caaatgaaca gcctgagagc tgaggacacg gctgtgtatt actgtgcgag agatcggagt 361 gggagctaca cctactttga ctactggggc cagggaaccc tggtcaccgt ctcctcagca 421 tccccgacca gccccaaggt cttcccgctg agcctctgca gcacccagcc agatgggaac 481 gtggtcatcg cctgcctggt ccagggcttc ttcccccagg agccactcag tgtgacctgg 541 agcgaaagcg gacagggcgt gaccgccaga aacttcccac ccagccagga tgcctccggg 601 gacctgtacg ccacgagcag ccagctgacc ctgccggcca cacagtgcct agccggcaag 661 tccgtgacat gccacgtgaa gcactacacg aatcccagcc aggatgtgac tgtgccctgc 721 ccagttccct caactccacc taccccatct ccctcaactc cacctacccc atctccctca 781 tgctgccacc cccgactgtc actgcaccga ccggccctcg aggacctgct cttaggttca 841 gaagcgaacc tcacgtgcac actgaccggc ctgagagatg cctcaggtgt caccttcacc 901 tggacgccct caagtgggaa gagcgctgtt caaggaccac ctgaccgtga cctctgtggc 961 tgctacagcg tgtccagtgt cctgtcgggc tgtgccgagc catggaacca tgggaagacc 1021 ttcacttgca ctgctgccta ccccgagtcc aagaccccgc taaccgccac cctctcaaaa 1081 tccggaaaca cattccggcc cgaggtccac ctgctgccgc cgccgtcgga ggagctggcc 1141 ctgaacgagc tggtgacgct gacgtgcctg gcacgtggct tcagccccaa ggatgtgctg 1201 gttcgctggc tgcaggggtc acaggagctg ccccgcgaga agtacctgac ttgggcatcc 1261 cggcaggagc ccagccaggg caccaccacc ttcgctgtga ccagcatact gcgcgtggca 1321 gccgaggact ggaagaaggg ggacaccttc tcctgcatgg tgggccacga ggccctgccg 1381 ctggccttca cacagaagac catcgaccgc ttggcgggta aacccaccca tgtcaatgtg 1441 tctgttgtca tggcggaggt ggacggcacc tgctactga // LOCUS F2978_2J12_kappa_1-13 927 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2J12 monoclonal IgA antibody kappa chain mRNA, complete cds. ACCESSION F2978_2J12_kappa_1-13 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 927) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 927) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..927 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 32..733 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MRVPAQLLGLLLLWLPGARCAIQLTQSPSSLSASVGDRVTITCR ASQGISSALAWYQQKPGKAPKLLIYDASSLESGVPSRFSGSGSGTDFTLTISSLQPED FATYYCQQFNSYPLTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFY PREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTH QGLSSPVTKSFNRGEC" BASE COUNT 223 a 278 c 218 g 208 t ORIGIN 1 gagtcagacc cactcaggac acagcatgga catgagggtc cccgctcagc tcctggggct 61 tctgctgctc tggctcccag gtgccagatg tgccatccag ttgacccagt ctccatcctc 121 cctgtctgca tctgtaggag acagagtcac catcacttgc cgggcaagtc agggcattag 181 cagtgcttta gcctggtatc agcagaaacc agggaaagct cctaagctcc tgatctatga 241 tgcctccagt ttggaaagtg gggtcccatc aaggttcagc ggcagtggat ctgggacaga 301 tttcactctc accatcagca gcctgcagcc tgaagatttt gcaacttatt actgtcaaca 361 gtttaatagt taccccctca ccttcggcca agggacacga ctggagatta aacgaactgt 421 ggctgcacca tctgtcttca tcttcccgcc atctgatgag cagttgaaat ctggaactgc 481 ctctgttgtg tgcctgctga ataacttcta tcccagagag gccaaagtac agtggaaggt 541 ggataacgcc ctccaatcgg gtaactccca ggagagtgtc acagagcagg acagcaagga 601 cagcacctac agcctcagca gcaccctgac gctgagcaaa gcagactacg agaaacacaa 661 agtctacgcc tgcgaagtca cccatcaggg cctgagctcg cccgtcacaa agagcttcaa 721 caggggagag tgttagaggg agaagtgccc ccacctgctc ctcagttcca gcctgacccc 781 ctcccatcct ttggcctctg accctttttc cacaggggac ctacccctat tgcggtcctc 841 cagctcatct ttcacctcac ccccctcctc ctccttggct ttaattatgc taatgttgga 901 ggagaatgaa taaataaagt gaatctt // LOCUS F2978_1G4_gamma1_5-51 1612 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1G4 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F2978_1G4_gamma1_5-51 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1612) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1612) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1612 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 58..1482 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MGSTAILALLLAVLQGVCAEVQLVQSGAEVKKPGESLKISCKGS GYSFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRYSPSFQGQVTISADKSISTAYLQW SSLKASDTAMYYCARLNFDYLSGYYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSS KSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPS SSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNV FSCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 353 a 533 c 432 g 294 t ORIGIN 1 ggtctccctc actgcccagc tgggatctca gggcttcatt ttctgtcctc caccatcatg 61 gggtcaaccg ccatcctcgc cctcctcctg gctgttctcc aaggagtctg tgccgaggtg 121 cagctggtgc agtctggagc agaggtgaaa aagcccgggg agtctctgaa gatctcctgt 181 aagggttctg gatacagctt taccagctac tggatcggct gggtgcgcca gatgcccggg 241 aaaggcctgg agtggatggg gatcatctat cctggtgact ctgataccag atacagtccg 301 tccttccaag gccaggtcac catctcagcc gacaagtcca tcagcaccgc ctacttgcag 361 tggagcagcc tgaaggcctc ggacaccgcc atgtattact gtgcgagact caattttgac 421 tacttatcgg ggtattatta ctactacggt atggacgtct ggggccaagg gaccacggtc 481 accgtctcct cagcctccac caagggccca tcggtcttcc ccctggcacc ctcctccaag 541 agcacctctg ggggcacagc ggccctgggc tgcctggtca aggactactt ccccgaaccg 601 gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt cccggctgtc 661 ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc cagcagcttg 721 ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa ggtggacaag 781 aaagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa 841 ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc 901 tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc 961 aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag 1021 gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg 1081 ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag 1141 aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca 1201 tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat 1261 cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc 1321 acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac 1381 aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac 1441 aaccactaca cgcagaagag cctctccctg tctccgggta aatgagtgcg acggccggca 1501 agcccccgct ccccgggctc tcgcggtcgc acgaggatgc ttggcacgta ccccctgtac 1561 atacttcccg ggcgcccagc atggaaataa agcacccagc gctgccctgg gc // LOCUS F2978_1G4_kappa_3-20 940 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1G4 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F2978_1G4_kappa_3-20 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 940) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 940) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..940 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 36..740 /codon_start=1 /product="immunoglobulin kappa chain" /translation="METPAQLLFLLLLWLPDTTGEIVLTQSPGTLSLSPGERATLSCR ASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPE DFAVYYCQQYGSSRGTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNF YPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC" BASE COUNT 223 a 282 c 235 g 200 t ORIGIN 1 gggaagagct gctcagttag gacccagagg gaaccatgga aaccccagcg cagcttctct 61 tcctcctgct actctggctc ccagatacca ccggagaaat tgtgttgacg cagtctccag 121 gcaccctgtc tttgtctcca ggggaaagag ccaccctctc ctgcagggcc agtcagagtg 181 ttagcagcag ctacttagcc tggtaccagc agaaacctgg ccaggctccc aggctcctca 241 tctatggtgc atccagcagg gccactggca tcccagacag gttcagtggc agtgggtctg 301 ggacagactt cactctcacc atcagcagac tggagcctga agattttgca gtgtattact 361 gtcagcagta tggtagctca cgggggactt tcggcggagg gaccaaggtg gagatcaaac 421 gaactgtggc tgcaccatct gtcttcatct tcccgccatc tgatgagcag ttgaaatctg 481 gaactgcctc tgttgtgtgc ctgctgaata acttctatcc cagagaggcc aaagtacagt 541 ggaaggtgga taacgccctc caatcgggta actcccagga gagtgtcaca gagcaggaca 601 gcaaggacag cacctacagc ctcagcagca ccctgacgct gagcaaagca gactacgaga 661 aacacaaagt ctacgcctgc gaagtcaccc atcagggcct gagctcgccc gtcacaaaga 721 gcttcaacag gggagagtgt tagagggaga agtgccccca cctgctcctc agttccagcc 781 tgaccccctc ccatcctttg gcctctgacc ctttttccac aggggaccta cccctattgc 841 ggtcctccag ctcatctttc acctcacccc cctcctcctc cttggcttta attatgctaa 901 tgttggagga gaatgaataa ataaagtgaa tctttgcacc // LOCUS F3005_2N5_gamma1_3-30 1643 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2N5 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3005_2N5_gamma1_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1643) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1643) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1643 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 82..1503 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLTWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAAS GFTFRTYGMHWVRQAPGKGLEWVAVISYDGSNKYYADSIKGRFTISRDNSKNTLYLQM NSLRAEDTAVYYCAKEGRGPYYYDSSGYFDYWGQGTLVTVSSASTKGPSVFPLAPSSK STSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSS SLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSL TCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 377 a 513 c 454 g 299 t ORIGIN 1 ggagctctgg gagaggagcc cagcactaga agtcggcggt gtttccattc ggtgatcagc 61 actgaacaca gaggactcac catggagttt gggctgacct gggttttcct cgttgctctt 121 ttaagaggtg tccagtgtca ggtgcagctg gtggagtctg ggggcggcgt ggtccagcct 181 gggaggtccc tgagactctc ctgtgcagcc tctggattca ccttcaggac ctatggcatg 241 cactgggtcc gccaggctcc aggcaagggg ctggagtggg tggcagtcat atcatatgat 301 ggaagtaata aatactatgc agactccata aagggccgat tcaccatctc cagagacaat 361 tccaagaaca cgctgtatct gcaaatgaac agcctgagag ctgaggacac ggctgtatat 421 tactgtgcga aggaagggag aggcccatat tactatgata gtagtggtta ttttgactac 481 tggggccagg gaaccctggt caccgtctcc tcagcctcca ccaagggccc atcggtcttc 541 cccctggcac cctcctccaa gagcacctct gggggcacag cggccctggg ctgcctggtc 601 aaggactact tccccgaacc ggtgacggtg tcgtggaact caggcgccct gaccagcggc 661 gtgcacacct tcccggctgt cctacagtcc tcaggactct actccctcag cagcgtggtg 721 accgtgccct ccagcagctt gggcacccag acctacatct gcaacgtgaa tcacaagccc 781 agcaacacca aggtggacaa gaaagttgag cccaaatctt gtgacaaaac tcacacatgc 841 ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt ccccccaaaa 901 cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt ggtggacgtg 961 agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga ggtgcataat 1021 gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt cagcgtcctc 1081 accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt ctccaacaaa 1141 gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc ccgagaacca 1201 caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt cagcctgacc 1261 tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag 1321 ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc 1381 tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc 1441 gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt 1501 aaatgagtgc gacggccggc aagcccccgc tccccgggct ctcgcggtcg cacgaggatg 1561 cttggcacgt accccctgta catacttccc gggcgcccag catggaaata aagcacccag 1621 cgctgccctg ggcccctgaa aaa // LOCUS F3005_2N5_kappa_1-33 936 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 2N5 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F3005_2N5_kappa_1-33 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 936) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 936) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..936 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 33..731 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MRVPAQLLGLLLLWLSGARCDIQMTQSPSSLSASVGDRVTITCQ ASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPSRFSGSGSGTDFTFTISSLQPED IATYYCQQYDNLPTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYP REAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQ GLSSPVTKSFNRGEC" BASE COUNT 244 a 268 c 217 g 207 t ORIGIN 1 ggagtcagtc ccaaccagga cacagcatgg acatgagggt ccctgctcag ctcctggggc 61 tcctgctgct ctggctctca ggtgccagat gtgacatcca gatgacccag tctccatcct 121 ccctgtctgc atctgtagga gacagagtca ccatcacttg ccaggcgagt caggacatta 181 gcaactattt aaattggtat cagcagaaac cagggaaagc ccctaaactc ctgatctacg 241 atgcatccaa tttggaaaca ggggtcccat caaggttcag tggaagtgga tctgggacag 301 attttacttt caccatcagc agcctgcagc ctgaagatat tgcaacatat tactgtcaac 361 agtatgataa tctgcccact ttcggcggag ggaccaaggt ggagatcaaa cgaactgtgg 421 ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct ggaactgcct 481 ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag tggaaggtgg 541 ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac agcaaggaca 601 gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag aaacacaaag 661 tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag agcttcaaca 721 ggggagagtg ttagagggag aagtgccccc acctgctcct cagttccagc ctgaccccct 781 cccatccttt ggcctctgac cctttttcca caggggacct acccctattg cggtcctcca 841 gctcatcttt cacctcaccc ccctcctcct ccttggcttt aattatgcta atgttggagg 901 agaatgaata aataaagtga atctttgtaa aaaaaa // LOCUS F3010_1I7_gamma1_1-18 1452 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1I7 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3010_1I7_gamma1_1-18 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1452) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1452) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1452 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1449 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MDWTWSILFLVAAATSAHFQVQLVQSGAEVKKPGASVKVSCKAS GYSFTNYGISWVRQAPGQGLEWMGWISAYNGNTNYAQKLQGRVTMTTDTSTSTAYMEL RSLRSDDTAVYYCARDGPFTVVVPTAIRDQVDYYYYGMDVWGQGTTVTVSSASTKGPS VFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSL SSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRE EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK" BASE COUNT 349 a 456 c 392 g 255 t ORIGIN 1 atggactgga cctggagcat ccttttcttg gtggcagcag caacaagtgc ccacttccag 61 gttcagctgg tgcagtctgg agctgaggtg aagaagcctg gggcctcagt gaaggtctcc 121 tgcaaggctt ctggttacag ttttaccaac tacggtatca gctgggtgcg acaggcccct 181 ggacaagggc ttgagtggat gggatggatc agcgcttaca atggtaacac aaactatgca 241 cagaaactcc agggcagagt caccatgacc acagacacat ccacgagcac agcctacatg 301 gagctgagga gcctgagatc tgacgacacg gccgtgtatt actgtgcgag agatggcccc 361 tttactgtag tagtaccaac tgctataagg gaccaagtag actactacta ctacggtatg 421 gacgtctggg gccaagggac cacggtcacc gtctcctcag cctccaccaa gggcccatcg 481 gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc 541 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 601 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc 661 gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac 721 aagcccagca acaccaaggt ggacaagaga gttgagccca aatcttgtga caaaactcac 781 acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 841 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 901 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 961 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1021 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1081 aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 1141 gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc 1201 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1261 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1321 ttcctctata gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1381 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtcc 1441 ccgggtaaat ga // LOCUS F3010_1I7_kappa_2-30 723 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1I7 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F3010_1I7_kappa_2-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 723) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 723) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..723 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..720 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MRLPAQLLGLLMLWVPGSSGDVVMTQSPLSLPVTLGQPASISCR SSQTLVYSDGSTYLSWFQQRPGQSPRRLIYKVSNRDSGVPDRFSGSGSGTDFTLKINR VEAEDVGVYYCMQGTHWPPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVC LLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVY ACEVTHQGLSSPVTKSFNRGEC" BASE COUNT 177 a 201 c 192 g 153 t ORIGIN 1 atgaggctcc ctgctcagct cctggggctg ctaatgctct gggtcccagg atccagtggg 61 gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccttggaca gccggcctcc 121 atctcctgca ggtctagtca aaccctcgta tacagtgatg gaagcaccta cttgagttgg 181 tttcaacaga ggccaggcca atctccaagg cgcctaattt ataaggtttc taaccgggac 241 tctggggtcc cagacagatt cagcggcagt gggtcaggca ctgatttcac actgaaaatc 301 aacagggtgg aggctgagga tgttggggtt tattactgca tgcaaggtac acactggccc 361 ccgtacactt ttggccaggg gaccaagctg gagatcaaac gaactgtggc tgcaccatct 421 gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcctc tgttgtgtgc 481 ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga taacgccctc 541 caatcgggta actcccagga gagtgtcaca gagcaggaca gcaaggacag cacctacagc 601 ctcagcagca ccctgacgct gagcaaagca gactacgaga aacacaaagt ctacgcctgc 661 gaagtcaccc atcagggcct gagctcgccc gtcacaaaga gcttcaacag gggagagtgt 721 tag // LOCUS F3010_1B11_gamma1_3-30 1410 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1B11 monoclonal IgG antibody heavy chain mRNA, complete cds. ACCESSION F3010_1B11_gamma1_3-30 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 1410) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 1410) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..1410 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..1407 /codon_start=1 /product="immunoglobulin heavy chain" /translation="MEFGLSWVFLVALLRGVQCQVQLVESGGGVVQPGRSLRLSCAAS GFTFSTYAMHWVRQAPGKGLEWVAVISYDGINKYYADSVKGRFSISRDNSKNTLYLQM NSLRAEDTAVYSCAQADSGGYYSSYYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGG TAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQ TYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMIS RTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQD WLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVK GFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSLSLSPGK" BASE COUNT 319 a 441 c 387 g 263 t ORIGIN 1 atggagtttg ggctgagctg ggttttcctc gttgctcttt taagaggtgt ccagtgtcag 61 gtgcagctgg tggagtctgg gggaggcgtg gtccagcctg ggaggtccct gagactctcc 121 tgtgcagcct ctggattcac cttcagtacc tatgccatgc actgggtccg ccaggctcca 181 ggcaaggggc tggagtgggt ggcagttata tcatatgatg gaattaataa atactatgca 241 gactccgtga agggccgatt cagcatctcc agagacaatt ccaagaacac tctttatctg 301 caaatgaaca gcctgagagc tgaggacacg gctgtgtatt cctgtgcgca ggccgatagt 361 gggggctact actcgtccta ctactggggc cagggaaccc tggtcaccgt ctcctcagcc 421 tccaccaagg gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc 481 acagcggccc tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg 541 aactcaggcg ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga 601 ctctactccc tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac 661 atctgcaacg tgaatcacaa gcccagcaac accaaggtgg acaagagagt tgagcccaaa 721 tcttgtgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg 781 tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag 841 gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac 901 gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc 961 acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag 1021 tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa 1081 gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg 1141 accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc 1201 gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg 1261 gactccgacg gctccttctt cctctatagc aagctcaccg tggacaagag caggtggcag 1321 caggggaacg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag 1381 aagagcctct ccctgtcccc gggtaaatga // LOCUS F3010_1B11_kappa_6-21 702 bp mRNA linear PRI 14-FEB-2022 DEFINITION Homo sapiens 1B11 monoclonal IgG antibody kappa chain mRNA, complete cds. ACCESSION F3010_1B11_kappa_6-21 VERSION KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 702) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Potent virus neutralization by human IgM antibodies against SARS-CoV-2 is eliminated by class switch JOURNAL unpublished REFERENCE 2 (bases 1 to 702) AUTHORS Callegari,I., Derfuss,T. and Sanderson,N.S.R. TITLE Direct Submission JOURNAL Submitted (14-FEB-2022) Department of Biomedicine, University of Basel, Hebelstrasse 20, Basel, 4031, Switzerland COMMENT Bankit Comment: ALT EMAIL:tobias.derfuss@usb.ch Bankit Comment: TOTAL # OF SEQS:38 ##Assembly-Data-START## Assembly Method :: EGassembler v. 2021 Sequencing Technology :: Illumina; Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..702 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 1..699 /codon_start=1 /product="immunoglobulin kappa chain" /translation="MLPSQLIGFLLLWVPASRGEIVLTQSPDFQSVTPKEKVTITCRA SQSIGNSLHWYQQKPDQSPKLLIKYASQSFSGVPSRFSGSGSGTDFTLTINSLEAEDA ATYYCHQSSSLPHTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYP REAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQ GLSSPVTKSFNRGEC" BASE COUNT 182 a 193 c 175 g 152 t ORIGIN 1 atgttgccat cacaactcat tgggtttctg ctgctctggg ttccagcctc caggggtgaa 61 attgtgctga ctcagtctcc agactttcag tctgtgactc caaaggagaa agtcaccatc 121 acctgccggg ccagtcagag cattggtaat agcttacact ggtaccagca gaaaccagat 181 cagtctccaa agctcctcat caagtatgct tcccagtcct tctcaggggt cccctcgagg 241 ttcagtggca gtggatctgg gacagatttc accctcacca tcaatagcct ggaagctgaa 301 gatgctgcga cgtattactg tcatcagagt agtagtttac cacacacttt cggcggaggg 361 accaaggtgg agatcaaacg aactgtggct gcaccatctg tcttcatctt cccgccatct 421 gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc tgctgaataa cttctatccc 481 agagaggcca aagtacagtg gaaggtggat aacgccctcc aatcgggtaa ctcccaggag 541 agtgtcacag agcaggacag caaggacagc acctacagcc tcagcagcac cctgacgctg 601 agcaaagcag actacgagaa acacaaagtc tacgcctgcg aagtcaccca tcagggcctg 661 agctcgcccg tcacaaagag cttcaacagg ggagagtgtt ag //