########################################################################################################################## #####################################################RaxML scripts######################################################## ########################################################################################################################## #Model test: GTRGAMMA: (100 runs) raxmlHPC-PTHREADS-SSE3 -s 60_teleosts_nt_aligned.fasta -p 76565454343434 -m GTRGAMMA -N 20 -T 30 -n ModelTest GTRCAT: (100 runs) raxmlHPC-PTHREADS-SSE3 -s 60_teleosts_nt_aligned.fasta -p 1767656545454552 -m GTRCAT -N 20 -T 30 -n ModelTest_4 #Initial rearrangement setting optimization: (20 runs each) raxmlHPC-PTHREADS-SSE3 -f d -i 10 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n FI_10 raxmlHPC-PTHREADS-SSE3 -f d -i 20 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n FI_20 raxmlHPC-PTHREADS-SSE3 -f d -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n AI6 #Number of categories optimization: (20 runs each) raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 10 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C10 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 40 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C40 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 45 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C45 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 50 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C50 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 55 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C55 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 60 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -N 10 -T 60 -n C60 raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 75 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -t RAxML_parsTree -T 40 -n C75 #Finding the best-known likelihood tree (BKT): raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 55 -p 767655454323 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 10 -T 40 -n BT0 raxmlHPC-PTHREADS-SSE3 -f d -d -i 10 -c 55 -p 987700011127 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 10 -T 40 -n BT10 raxmlHPC-PTHREADS-SSE3 -f o -i 10 -c 55 -p 443326776565000 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 10 -T 40 -n BT20 raxmlHPC-PTHREADS-SSE3 -f o -d -i 10 -c 55 -p 44335000 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 10 -T 40 -n BT30 #Bootstrapping: raxmlHPC-PTHREADS-SSE3 -f d -i 10 -c 55 -p 8121123 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 100 -b 76543434 -T 40 -n BS0 raxmlHPC-PTHREADS-SSE3 -f o -i 10 -c 55 -p 8776429 -m GTRCAT -s 60_teleosts_nt_aligned.fasta -N 100 -b 81010101 -T 40 -n BS20 Ancestral sequence prediction: Based on the calculated BKT: raxmlHPC-PTHREADS-SSE3 -f A -t 60_teleost_BT_rooted_nt_newick.txt -s 60_teleosts_nt_aligned.fasta -m GTRCAT -i 10 -c 55 -n ASR_nt Based on the species tree published previously: raxmlHPC-PTHREADS-SSE3 -f A -t 73g_nucl_conc_fossils.combined_latinnames.nex -s 60_teleosts_nt_aligned.fas -m GTRCAT -i 10 -c 55 -n ASR_nt_species ########################################################################################################################## ##################################################MrBayes input files##################################################### ########################################################################################################################## #NEXUS begin taxa; dimensions ntax=75; taxlabels Acanthochaenus_luetkenii Anabas_testudineus Antennarius_striatus Arctogadus_glacialis Astyanax_mexicanus Bathygadus_melanobranchus Benthosema_glaciale Beryx_splendens Boreogadus_saida Borostomias_antarcticus Brosme_brosme Brotula_barbata Carapus_acus Chaenocephalus_aceratus Chatrabus_melanurus Chromis_chromis Cyttopsis_roseus Danio_rerio Gadiculus_argenteus Gadus_morhua Lampetra_tridentata Gasterosteus_aculeatus Guentherus_altivela Helostoma_temminckii Holocentrus_rufus Laemonema_laureysi Lampris_guttauts Lamprogrammus_exutus Lesueurigobius_cf_sanzoi Lota_lota Macrourus_berglax Malacocephalus_occidentalis Melanogrammus_aeglefinus Melanonus_zugmayeri Merlangius_merlangus Merluccius_merluccius Merluccius_polli Molva_molva Monocentris_japonica Mora_moro Muraenolepis_marmoratus Myoxocephalus_scorpius Myripristis_jacobus Neoniphon_sammara Oreochromis_niloticus Oryzias_latipes Osmerus_eperlanus Parablennius_parvicornis Parasudis_fraserbrunneri Perca_fluviatilis Percopsis_transmontana Phycis_blennoides Phycis_phycis Poecilia_formosa Pollachius_virens Polymixia_japonica Pseudochromis_fuscus Rondeletia_loricata Salmo_salar_1 Salmo_salar_2 Sebastes_norvegicus Selene_dorsalis Spondyliosoma_cantharus Stylephorus_chordatus Symphodus_melops Takifugu_rubripes Tetraodon_nigroviridis Theragra_chalcogramma Thunnus_albacares Trachyrincus_murrayi Trachyrincus_scabrus Trisopterus_minutus Typhlichthys_subterraneus Xiphophorus_maculatus Zeus_faber ; end; begin characters; dimensions nchar=711; format datatype=dna missing=? gap=-; matrix Acanthochaenus_luetkenii ATGATTACAAAACTA---------GACCGTGTGCTTTTGGCCAAGGAAACGTTCATCTTCCATTAT GAGAACATGCGCTGGGCAAAAGGTCGGCATGAGACATACCTCTGCTTTGTAGTGAAGAGGCGGGTGGGGCCAGACTCCCTGTCCTTTGACTTTGGACACCTCCGCAAC------------------CGCACT GGCTGCCAT GTAGAGCTGCTGTTCCTGCGCCACCTG------GGAACCTTGTGCCCTGGACTGT GGGGGTACGGAGGCGCTGGAGAG---AGGAGGCTCAGTTACTCCATCACCTGGTTCTGC TCC TGGTCCCCCTGCGCTGACTGCGCCTTCAGAGTGGCCCAGTTAATCGGCCGGACG---------CCC AACCTCCGCCTCAGGATCTTCGTCTCTCGCCTCTACTTCTGCGACC TGGAGGACAGCCGCG AGAGAGGGGGCCTGAGGTTGCTGAAGAAAGCTGGCGTGCAGATCACTGTCATGAGCTACAAAGACTTTTTCTATTGCTGGCAGACCTTTGTGGCTAATGGAGGGAGCAGCTTCAAGGCCTGGGACGAGATGCACCAAAACTCTGTTCGCCTGGCCAGC---------CAA---CTCAACCACATCCTG CAGCCATGTGATACAGAGGAC TTAAGAGATGCATTCAAGCTTCTTGGTCTG------------------TGA Anabas_testudineus ATGATTACAAAGCTA---------GACAGTGTGCTTTTGCCCCGAAAGAAGTTTATCTACCATTAC AAGAATGTGCGCTGGGCGAGGGGTCGTCATGAAACATACCTCTGTTTCGTAGTGAAGAGGCGGGTGGGCCCAGACTCCTTGACCTTTGACTTTGGACACCTCCGCAAT------------------CGCAAT GGCTGCCATGTGGAGATGCTGTTCTTGCGCTATCTG------GGAGCCTTATGTCCTGGTATTTG GGGGTACGGAGGTGCTGGAGAG---AAAAGGCTCAGTTACTCAATTACCTGGTTCTGTTCCTG GTCTCCTTGTGCCAACTGCTCCCTTAGGCTGACCCAGTTCCTCAGTCAGACC---------CCCAAC CTCCGCCTCAGGATCTTTGTGTCCCGCCTTTACTTCTGTGACATGGAGGACAGCCGCGAGCGGGAGGGTCTGAGGATACTGAAAAATGCTGGCGTGCAGATCACAGTCATGACTTACAAAGACTTCTTCTATTGCTGGCAGACCTTTGTGGATCGTAAACAGAGCAGCTTCAAAGCGTGGGATGAGCTGCACCAAAACTCTGTTCGCCTCACCAGA---------AAA---CTCTACCGCATCCTTCAGCCC TGTGAAATAGAAGATTTAAGAGATGCCTTCAAGCTTCTTGGGCTG------------------TGA Antennarius_striatus ATGATTACGAAGCTT---------GACAGCGTGCTTTTGCCCCGAAAAAAGTTCATCTACCATTAT AAGAACATGCGCTGGGCGAGAGGCCGGTGTGAGACGTACCTCTGCTTTGTAGTGAAGAGACGAGAGGGGCCAGACACCTTAACTTTTGACTTTGGACACCTCCGTAAT------------------CGCAAT GGCTGTCATGTGGAGCTACTTTTCTTACGCTATCTG------GGGGCCTTGTGCCCTGGATTGTG GGGCAGTGGGGGTACTGGGGAG---AAGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGTGCCAACTGTTCCATCAGACAGTGTGAATTCCTGAGCCGAACG---------CCCAA CCTTCGCCTCAGGATCTTTGTCTCTCGTTTGTACTTCTGCGACCTGGAGGATAGCCGTGAAAGGGAAGGCCTAAGAATGCTGAAGAAAGCCGGCGTGCAGATCTCAGTCATGAGTTACAAAGACTTCTTCTACTGCTGGCAGACCTTTGTGGCTAGTAAACAAAGTAGTTTCAAGGCTTGGGAAGAGCTGCATCAAAATTCAGTACGCCTTGCCAGA---------AAA---CTGAACCGCATCCTCCAGC CGTGTGAAGCTGAAGATTTAAGAGATGCCTTTAAGCTTCTTGGACTG------------------TGA Arctogadus_glacialis ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAAAATAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAATGAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGCTGCGCAGACGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGACCCGGTTCCTGAGGCAGACA---------CCAA ACCTGCGACTCAGGATCTTCGTGTCTCGCCTCTACTTTTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAAAA?C?AAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Astyanax_mexicanus ATGACGAGCAAGCTG---------GACAGCATTCTGCTCACCCAGAAGAAGTTTATCTATCACTAC AAGAACGTGCGCTGGGCTCGTGGGAGGCATGAGACTTACCTCTGCTTCGTGGTGAAGAGGCGAATCGGACCAAACTCGCTGTCCTTCGACTTCGGGCACCTGCGCAAC------------------CGCTCC GGCTGCCACGTGGAGCTCCTCTTCCTGCGCTACCTG------GGGGCACTGTGCCCGGGCCTGG GGGGTCTGGGTGTGGACGGAGTG------AAGGTGGGCTACGCTGTGACCTGGTTCTGCTCATG GTCGCCCTGCTCTAACTGCGCCCAGCGAATCGCCCACATCCTGTCCCAGACG---------CCCAG CCTGCGACTCCGCATCTTCGTCTCCCGCCTGTACTTCTGCGACAACGAGGACAGCCTGGAGCGGGAGGGGCTGCGGCACCTGCTGAGGGCAGGGGTGCAGATTACAGTCATGACGTATAAAGATTTTTTCTACTGTTGGCAGACGTTTGTGGCTCGCAGGGAGAGTCGCTTTAAAGCCTGGGACGGTCTTCACCAAAACTCTGTCAGACTGTCCCGC---------AAA---CTCAAACGCATCCTCCAGCC CTGTCAGACTGAAGATCTGAGGGACGTCTTCGCTCTGCTGGGTCTC------------------TGA Bathygadus_melanobranchus ATGATTAGTAAGCTC---------GACAGTGTGCTTTTGGCCCAGAAAAAATTCATGTACAATTAC AAGAACGTGCGCTGGGCAAAAGGCCGCCACGAGACCTACCTCTGCTTCGTAGTGAGGAGAAGGCTCGGACCAAATTCCCTGTCTTTTGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCGCTCTGCCCAGGCCTCT GGGGGTGCGTAGGTGATGACAAC---AGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGCGCCAACTGTGCGGCCACACTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGACAGTCCGAATATAGAGGGCTTGAGAGAGCTGAGGAGGGCAGGAGTCCAGGTCATCGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACATTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGAGTTATTGGGCTGTTAAGC------------TGA Benthosema_glaciale ATGATTACTAAACTA---------GACAGTGTGCTTTTGGGTCAGAAGAAGTTCCTCTTCCACTAT AAGAACGTGCGCTGGGCGTGGGGTCGAAATGAAACGTACCTCTGCTTTGTGGTGAAGAGGAGAGTAGGACCAAACTCCCTCTCCTTTGACTTTGGACATCTCCGCAAC------------------CGCTCC AGCTGCCACGCGGAGCTGCTGTTCCTTCGCCACCTG---GGGGGCGCCCTGTGCCCTGGTCTG TGGGGCTACGGAGGTGACGGGGGAGAGGGGAGGTTCAACTACTCGGTCACCTGGTTCTGCTCGTGGTCTCCGTGCGCCGACTGTTCTCTGAGACTGGCCCAGTTCCTCAGCCGGACC---------C CCAACCTGCGCCTCCGCATCTTCGTCTCTCGCCTCTACTTCTGTGACGCGGAGGACAGCCGGGAGAGGGAGGGTCTGAGGACGCTGAAAAGGGCAGGTGTACAGATCACCGTCATGAACTACAAAGACTACTACTATTGTTGGCAGACCTTTGTGGCTCACAGACAGAGCAGCTTCAAGGCCTGGGCTGATCTGCACCAGAACTCTGTCCGTCTGGCCAGG---------AAA---CTCCACCGCATCCTC CAGCCTTGTGAGACAGAGGATTTTAGAGACGCATTCAAGCTTCTTGGGTTG------------------TGA Beryx_splendens ATGATTACAAAACTA---------GACAGTGTGCTTTTGGCCAAGAAAAAGTTCATCTACCATTAC AAGAACATGCGCTGGGCAAAGGGCCGGCATGAGACATACCTCTGCTTTGTGGTGAAGAGGCGAGTGGGGCCAGACTCCCTGTCCTTCGACTTCGGACACCTCCGCAAC------------------CGCGC TGGCTGCCATGTAGAGCTGCTGTTCCTGCGCCACCTG------GGAGCCCTGTGCCCTGGACTGT GGGGGCATGGAGGCAGCGGAGAG---AGGAAGCTGAGTTACTCCATCACCTGGTTCTGCTCC TGGTCTCCCTGCGCTGACTGCTCCTTCAGACTGGCCCAGTTCCTCAACCGGACG---------CCC AACCTCCGCCTCAGGATCTTCGTCTCCCGCCTCTACTTCTGCGACCAGGAGGACAGCCGCGAGAGAGACGGCCTGAGGCTGCTGAAAAAGGCCGGCGTGAACATCACTGTCATGAGCTACAAAGACTTCTTCTACTGCTGGCAGACCTTTGTGGCTAACAGAACGAGCAGATTCAAGGCCTGGGATTTGCTGCACCAAAACTCTGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCA GCCTTATGAGATAGAAGATTTAAGAGATGCCTTCAGACTTCTTGGTTTT------------------TGA Boreogadus_saida ATGATTAGGAAGCTA---------GACAGTGTGCTCTTGGCCCAGAATAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTCGACTTCGGACACCTACACAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGCTGCGCAGACGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGACCCGGTTCCTGAGGCAGACA---------CCAA ACCTGCGACTCAGGATCTTCGTGTCTCGCCTCTACTTTTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCCGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGC?TCTGTCAAGA---------AAA---CTAAACCGCATCCTCCAG CCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Borostomias_antarcticus ATGATCAGTAAACTA---------GACAGTGTTCTCCTGGCCCAGAAGAAGTTCCTCTTCCACTAC AAGAACGTGCGCTGGGCCCGAGGACGACACGAGACGTACCTGTGCTTCGTGGTGAAGAGGAGGGTGGGACCCGACTCGCTTACCTTCGACTTCGGACACCTGCGCAAT------------------CGCA CCGGCTGCCACGTTGAGCTGCTGTTCCTGCGCCATCTA------GGGGTGCTGTGTCCGGGCCTG TCGGCGTCTGGAGGTGCTGGAGGGGGCAGGGGGCTGAACTACTCCATCACCTGGTTCTGCTCATGGTCCCCCTGCTTCGACTGCTCGGCCCGGCTGGCCCAGTTCCTGAGACGGACC---------C CCAACCTCAGGCTCCGCCTCTTCGTCTCCCGCCTCTACTTCTGTGACCCGGAGGACCGCCACGAGAGAGAGGGGCTCCGGGCGCTGAAGAGAGCCGGAGTCCACATCACCGTCATGAGCTATAAAGATTATTTTTACTGCTGGCAGACGTTTGTAGCTCACAGACAGAGGGCCTTCAAAGCCTGGGAAGATCTTCAGCAGAACTCCGTCCGCCTGGCCAGG---------AAG---CTCAACAGCATCCT GCTGCCCTGTGAGACGGAGGATCTGAGAGACCCGTTCAGGCTGCTTGGACTG------------------TGA Brosme_brosme ATGATGAGTAAGCTA---------GACAGTGTGCTCTTAGCCCAGAAGAAATTCATATACAATTAC AAGAACCTGCGATGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTCGGACCTGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGTAC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCTACCTG------GGGGCGCTGTGCCCAGGCCTCT GGGGGTGCGGTGGCGACAGAAAC---CAAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCACACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATATAGAGGGCTTGAGGGACCTGAGGAGAGCAGGGGTCCAGGTCAAAGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGACTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCAAGA---------GCT---CTAAACCGCATCCTCCAG CCATGTGAAACAGAAGATTTAAGAGATCCTTTCAGACTTTTTGGACTGTTAACC------------TGA Brotula_barbata ATGATTGCGAAACTA---------GACAGTGTACTTTTACCACGGAAAAAGTTCATCTACCATTTC AAGAACATGCGCTGGGCTAAGGGTCGGCATGAGACGTACCTGTGCTTTGTGGTGAAGAGGCGAGTAGGGCCGGACTCGCTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCAAT GGCTGCCACGTAGAGCTACTGTTCTTACGCTACCTA------GGAGCTTTATGCCCTGGACTGTG GGGCTGTGGGAATTCTGGACAG------AGGTTGTGTTACTCCATCACTTTGTTCTGCTCTTGGT CCCCCTGTGCCAACTGTTCCGAGAGACTGGCCAAGTTCCTCGGCCGGACA---------CCCAACC TTCGCCTCAGGATCTTTGTCTCTCGCCTCTACTTCTGCGACATGGAAGACAGCCGTGAAAGAGAGGGTCTGAGGATGCTGAAAAATGCTGGCGTAAACATCACAGTCATGAGCTACAAAGACTATTTCTATTGCTGGCAAACCTTTGTGGCTCGTGGCGCAAGCAACTTCAAAGCCTGGGATGGGCTGCAAGAGAATTCAATTCGCCTTGCCAGG---------AAA---CTCACCCACATCCTACAGCCA GGTGAGACGGAAGATTTAAGGGACGCATTCAAACTTCTGGGTATG------------------TGA Carapus_acus ATGACTGCCAAGCTA---------GACAGGGTCCTTTTGCCACGGAAAAAGTTCCTCTTCCATTAC AAGAACGTGCGCTGGGCGAAGGGCCGCCACGAGACGTACCTCTGCTTCGTGGTGAAGAGGCGAGTGGGTCCAGACTCCATGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCAG TGGCTGCCACGTAGAGCTCTTGTTCCTGCGCTACCTG------GGAGCTCTGTGTCCTGGACTGT GGGGGTATGAAGGTTCTGGACAG---AGGAGACTCAGCTACTCCATCACCTGGTTCTGCTCTT GGTCCCCGTGCGCCAACTGCTCGGAGCGACTCGCCCAGTTCCTCAATCGGACC---------CCCA ACCTCCGCCTCAGGATCTTCGTCTCTCGCCTCTACTTCTGCGACCTGGAGGACAGCCGTGAGAGGGAGGGCCTGAGGACGCTGGAGAAAGCTGGCGTGCACATCACCATCATGAGCTACAAAGACTATTTCTACTGCTGGCAAACCTTTGTGGCTTGTGGAACTTCAAAATTCAAAGCCTGGGATGAGCTCCACCAAAACACCACTCGTCTCAAGAGA---------AAA---CTGAATCGGATCCTCCAG CCATGTGAGACAGAAGATTTAAGGGACGCATTCAAACTTCTAGGGTTGCTG---------------TGA Chaenocephalus_aceratus ATGATCACAAAGCTT---------GACAGCATGCTTTTGCCTCGAAAAAAGTTCATCTACCATTAC AAGAACATGCGCTGGGCAAGGGGCCGGTGTGAGACATACCTCTGCTTTGTAGTGAAGAGGCGGGTGGGACCAGACTCCTTAACCTTTGACTTCGGACACCTTCGCAAT------------------CGCAAT GGCTGCCATGTAGAGATGCTGTTCCTGCGCTACCTG------GACGCCCTGTGCCCTGGTCTGTT GGGATGTGAAGGTACTGGAGAG---AAGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCCCCCTGTGCAAACTGCTCCATCAGGCTGTCCCAGTTCCTCAGCCAGACG---------CCCAA TCTTCGCCTCAGGATCTTCGTCTCTCGTCTTTACTTCTGTGACATGGAGAATAGCCCTGCAAGAGACGGCCTAATAATGCTGAAAAAAGCTGGCGTGCAGACTTCAGTCATGAGTTACAAAGACTTTTTCTATTGCTGGCATAACTTTGTGGATTGTAAACAGAGTAAATTCAAGCCATGGGAAGATCTGCACCAAAACTCTGTTCGCCTTGCCAGA---------AAA---CTCAAACGCATCCTTCAGCT GTGTGAAACTGAAGATTTGAGAGATGCCTTCAAGCTTCTTGGACTG------------------TAA Chatrabus_melanurus ATGATTACAAAACTA---------GACAGTGTGCTTTTGCCACGGAAGAAGTTCATCTACCATTAC AAGAACATGCGCTGGGCAAAGGGCCGGCACGAGACATACCTCTGCTTTGTGGTGAAGAGACGAATGGGGCCAGACTCCCTGTCCTTTGATTTCGGACACCTCCGCAAT------------------CGCAA CGGCTGCCATGTAGAGCTGCTGTTCCTGCGTTACCTG------GGAGCCTTGTGCCCTGGTCTGT GGGGGTATGGAATTGCTGGAGAG---AGGAAGCTTAGTTACTCCGTCACCTGGTTCTGCTCCT GGTCCCCCTGTGTCAACTGCTCCCTCAGACTGACACAGTTCCTCATGCAGACG---------CCTA ATCTTCGCCTCAGGATCTTCGTCTCTCGCCTTTACTTCTGTGATATGGAAGACAGCCGTGAGAGAGAAGGTCTGAGGATGCTGAAAAAAGCCGGCGTGCACATCACAGTGATGAGTTACAAAGACTTCTTCTACTGCTGGCAGACCTTTGTGGCTTGTAAAGAGAGCAAATTCAAGGCATGGGAGGCGCTGCACCAAAACTCTGTTCGTCTGGCTAGA---------AAG---CTCAACCGCATCCTCCA GCCCTGTGAGACAGAAGACTTCAGAGATGCCTTCAAGCTTCTTGGACTG------------------TGA Chromis_chromis ATGATCACAAAACTC---------GACAGTGTGCTTTTGCCCCAGAAGAAGTTCATCTACCATTAT AAGAACATGCGCTGGGCGAGAGGCCGCTGTGAGACGTACCTCTGCTTCGTGATTAAGAAAAGAGCCGGTCCAGATTCTATATCCTTCGACTTCGGACATCTACGGAAC------------------CGCAAC GGCTGCCATGTAGAGCTGCTGTTCCTGCGCTACCTG------GGCGCCTTGTGTCCTGGTCTCTG GGGTTATGGACAG------------AACCGGATCAGCTACTCCATCACCTGGTTCTGCTCCTGGTCTC CCTGCGCTAACTGCTCCCTCAGACTGGCCCAGTTCCTGAACCAGACG---------CCCAACCTTC GTCTCCGGATCTTCGTCTCTCGGCTCTACTTCTGCGACATGGAGGACAGCCGGGAGAGGGAAGGTCTGAGGATCCTGAAGAAGGCCGGCGTTAACATCACCGTCATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTGGCTCGGAGGCTGAGTAAGTTCAAACCGTGGGACGGGCTGCAACAGAACTACGTCCGTCTGTCCAGA---------AAA---CTGAACCGCATCCTGCAGCCCTG TGAGACTGAAGACTTTCGAGACGCCTTCAGGCTCCTTGGACTC------------------TGA Cyttopsis_roseus ATGATTACTAAACTA---------GACAGTGTGCTTCTGGCTCGGAAGACATTCATTTACCACTAT AAGAACATGCGCTGGGCAAAAGGCCGGCATGAGACATACCTCTGCTTCGTCGTCAAGAGAAGAGTTGGACCCGATTCCTTGTCCTTTGACTTTGGACACCTTCGCAAT------------------CGGAC TGGCTGCCATGTAGAGCTCCTGTTTCTACGTCACCTG------GGGGCCCTGTGCCCTGGACTGT GGGGACAAGGAGGCGCTGATGAA---AGAAGGCTCAGTTACTCGGTCACCTGGTTCTGCTCC TGGTCTCCCTGCGCCAACTGCTCCCTCAGACTGGTCCAATTCCTCGGGCAGACG---------CCC AACCTCCGTCTCAGGATCTTCGTCTCCCGTCTCTACTACTGTGACCTTGAAGACAGCCGCGAGAGAGAGGGCTTAAGAACCCTGAAAAGAGCCGGAGTCCAAATCACAGTCATGAGCTACAAAGACTATTTCTATTGCTGGCAGACGTTCGTGGCTCGCCGACAGACCCGCTTCAAGGCGTGGGATGAGCTGCACCAAAACTCAGTTCGTCTGGCCAGG---------AAA---CTAAACCGCATCCTCC AGCCTTGTGAAACGGAAGATTTAAGAGATGCTTTCAAACTTCTCGGGTTCTTG---------------TAA Danio_rerio ATGATCTGCAAGCTG---------GACAGTGTGCTCATGACCCAGAAGAAATTCATCTTCCACTAT AAGAATGTGCGCTGGGCTCGAGGGAGACACGAAACCTACCTTTGTTTTGTAGTAAAGCGACGCATCGGCCCTGATTCCCTCTCTTTTGACTTTGGACACCTGCGCAAT------------------CGCTCC GGATGCCATGTAGAGCTTCTCTTTCTGCGTCACTTG------GGTGCGTTGTGTCCGGGCCTGAG CGCTTCCAGTGTGGACGGTGCA------AGATTGTGTTACTCAGTGACCTGGTTCTGCTCCTGGT CGCCCTGCTCTAAATGCGCTCAACAGCTCGCCCACTTCCTGTCACAGACG---------CCCAATC TGAGGCTGAGGATCTTTGTGTCACGCCTGTACTTCTGTGATGAAGAGGACAGCGTGGAGAGAGAAGGTCTGCGACACCTGAAGAGGGCAGGAGTTCAGATCTCGGTCATGACTTATAAAGACTTTTTCTACTGCTGGCAAACGTTTGTTGCGAGGAGGGAGCGGAGTTTTAAAGCCTGGGATGGACTTCATGAAAACTCTGTCCGGCTTGTTCGG---------AAA---CTCAATCGGATTCTGCAGCCT TGCGAGACTGAGGATCTGAGGGATGTTTTTGCTCTTCTTGGGTTA------------------TGA Gadiculus_argenteus ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATATACAATTAC AATAACATGCGATGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTCGTCGTGAAGAGAAGGCTTGGACCTGACTCCCTCTCCTTCGACTTCGGACACCTACGCAAT------------------CGCAC CGGCTGCCACGCAGAGGTGCTGTTCCTGAGCTACCTC------GGGGCACTGTGTCCGGGCCTCT GGGGCTGCGCAGGCGACAGAAGC---CTAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTCGCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTGGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTGCGTCTGTCAAGG---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Gadus_morhua ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAAAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAGTAAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTTGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTTTTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGCTGCGCAGACGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGACCCGGTTCCTGAGGCAGACA---------CCCA ACCTGCGACTCAGGATCTTCGTGTCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Lampetra_tridentata ATGGCCAACGATGAGTACGTGAGAGTCGGCGATAAGTTGGACAGCTGCACGTTTAGGACGCAGTTTTTTAACTTTAAAAGATCCACGTCG---CATATATGCTGCGTTCTCTTTGAATTAAAACA GCAGGATAGCGTCGCT------------TTTTGGGGCTATGCTGTGAATAAACCACGGAGCAATGCA GACCTAGGAATTCACGCCGAAATTTTTTGCATTAAAAAAATC------AGAGAGTAC---------------------------CTGCACGAA---AACCCTGGAATATACACGATAAATTGGTACTCATCCTGGAGTTCG TGTGCAGATTGCGCTGAAGAGATCTTAACATGGTATAAGAAGGAGGTGATGAAGATGGGCCACACTTTGAATATCTGGGCTTGCAAACTCTATTTCGAGAACATT------ACGCGGAATCAAAT TGGGTTGTGGAACCTCAGAAAAATCGGGGTTGGGTTGGAAATAATGCTTGGTGAACACTACCAATGGTGCTGGAACAACTACATCCAAACGTTGGACAGCAATTTGAATGAAAATAGATGGCTTCAGAAGACTTCGAATCGAGCTCTTACACGACAGAACGAG---TTGTCCATTATGATTCAG------------------------------GTAAAAAGACTCCACACCGCTAAGACTCCTGCTGTTTAG Gasterosteus_aculeatus ATGATTGCAAAGCTT---------GACAGTGTGCTTCTGCCCCGAAAAAAGTTCATCTACCACTAC ACGAACATGCGCTGGGCGAGGGGCCGACACGAGACTTACCTCTGCTTTGTTGTGAAAAGGCGAGTGGGGCCGGATTCCTTGTCCTTCGACTTTGGACACCTGCGCAAT------------------CGCAGT GGCTGCCATGTCGAGTTGTTGTTCCTGCGCCACCTC------GGAGCCTTGTGCCCTGGTTTCTT GGGTTGTGGAGACACCGGAGGG---AGGAGGCTGAGTTACTCCATCACCTGGTTCTGCTCGTG GTCTCCCTGCGTAAACTGCTCCATCAGTCTGTCCCAGTTCCTCAGCCGGACG---------CCCAA CCTCCGCCTCAGGATCTTCGTCTCTCGCCTTTACTTTTGTGACATGGAGAACAGTCGGGAAAGAGACGGCCTGAGAATGCTGAAAAAAGCTGGCGTGCAGGTCACAGTCATGAGTTACAAAGATTTCTTCTATTGCTGGCAGACTTTTGTAGATCGCAAACAAAGCCAGTTCAAGGCCTGGAAAGAGCTTCACCAAAACTCTGTTCGCCTTTCCAGA---------AAG---CTCAAGCGCATCCTCCAGCC TTGTGAAACAGAAGATTTAAGGGATGCCTTCAAGCTGCTTGGACTG------------------TGA Guentherus_altivela ATGATTACTAAACTA---------GACAGCATACTTATGGCCCAGAAGAAGTTCATCTTCCACTAT AAGAACATGCGATGGGCCAAGGGTCGAAATGAGACACACCTCTGCTTTGTGGTGAAGAGAAGGCTGGGACCAAACTCCCTGTCCTTTGACTTTGGACACCTGCGTAAT------------------CGCAC TGGCTGCCATGTAGAGCTACTCTTCTTGCGCCACCTG------GGATTCCTGTGCCCTGGCTTGT GGGGGTACGGAGAGCCAGGTGAA---GGGAGGCTGAATTACTCTGTCACCTGGTTCTGCTCCT GGTCCCCCTGTGCAGATTGTTCCTTCACGCTGACCCACTTCCTCAGAGAGACT---------CCCA ACCTCCGTCTTAGAATCTTTGTGTCTCGCCTCTACTTCTGTGACGAGGAGGACAGCAGTGCAAGGGAAGGCCTGCGAATGTTGAAGAAAGCCGGTGTGAACATCACTGTCATGAGCTACAAAGACTACTTCTATTGCTGGAAGACCTTTGTGGCTCACAGACAAAGGAACTTCAAGGCCTGGGATGGGCTAGACCAGAACTCTGTTCACCTAGCCAGG---------AAA---CTCAGCCACATCCTCCA GCCCTGGGAAACAGCAGATTTAAGAGATGCCTTTAAACTTCTTGGACTG------------------TGA Helostoma_temminckii ATGATTACAAAGCTA---------GACAGTGTGCTTTTGCCCCGAAAAAAGTTCATCTACCATTAC AAAAATGTGCGCTGGGCAAGGGGTCGGCATGAGACATACCTCTGTTTTGTAGTGAAGAGGCGGGTGGGCCCAGACTCCTTGACCTTTGACTTTGGGCATCTCCGCAAT------------------CGCAAT GGTTGCCATGTAGAGATGCTGTTCTTGCGATATCTG------GGAGCTTTGTGCCCTGGACTTTG GGGGTGTGGAGGTACTGGAGAG---AGAAGGCTCAGTTACTCTATTACCTGGTTCTGCTCCTG GTCTCCTTGTTCTAACTGCTCCCTTAGACTGGCCCAGTTCCTCAGTCAGACC---------CCAAAC CTCCGCCTCAGGATCTTTGTGTCTCGCCTATACTTCTGTGACATGGAGGACAGTCGCGAGAGGGAGGGTCTCAGGATCCTGAAAAACGCTGGAGTGCAGATCACAGTCATGAGTTACAAAGACTTCTTCTACTGCTGGCAGACATTTGTGGCACGTAAGCAGAGCAACTTCAAAGCATGGGAGGAGCTGCACCAAAACTCTGTTCGCCTTACCAGA---------AAA---CTCCATCGCATCCTTCAGCC TTGTGAAACAGAAGATTTAAGAGATGCTTTCAAGCTCCTTGGACTG------------------TGA Holocentrus_rufus ATGATTACAAAACTA---------GACAGTGTGCTTTTGGCCAAGAAAAAGTTCATCTACCATTAT AAGAACTTGCGCTGGGCAAAAGGCCGGCATGAGACATACCTCTGCTTTGTCGTGAAGAGGCGGGCGGGGCCGGACTCCATCGCCTTCGACTTTGGACACCTCCGCAAC------------------CGTGCT GGCTGCCATGTAGAGCTGCTATTCCTTCGCTACCTG------GGAGCCTTGTGCCCTGGACTGTG GGGCTACGGAGGAACTGGTGAG---AGGAAGATGAGCTACTCCATCACATGGTTCTGCTCCT GGTCTCCTTGTGCCAACTGCTCCTACAGACTCGCCCAGTTCCTCAACCGGACG---------CCCA ACCTCCGCCTCAGGCTCTTCGTCGCTCGCCTCTATTTCTGTGACATCGAGGACAGCCGTGAGAGAGAGGGCCTGAGAATGCTGAAGAATGCCGGTGTGCACATCACTGTCATGAGCTACAAAGACTACTTCTACTGCTGGCAGACATTTGTGGCTCGTAAAACGAGCAACTTCAAGGCCTGGGATGGGCTGCACCAAAACTATGTTCGTCTGGCCAGG---------AAA---CTCAACCGCATCCTCCA GCCTTGTGAGACAGAAGATTTAAGAGATGCATTCAGGCTTCTTGGCTTG------------------TGA Laemonema_laureysi ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATGTTCAATTAC AAGAACATGCGCTGGGCAAGAGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCAATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC AGGCTGCCATGTAGAGCTGCTGTTTTTGAGCTATCTG------GGGGCACTGTGCCCAGGCCTGT GGGGGTGCAGAGGCGACGAAAAC---AGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCC TGGTCTCCATGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCC AACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGACAGTCCCCATATAGAGGGCTTGAGGGACCTGAGGAGAGCAGGGGTGCGGGTCACCGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGGCTGTTAACC------------TGA Lampris_guttauts ATGATCAGCAAACTA---------GACAGTGTGCTTCTGACCCAGAAGAAGTTCCTCTACCATTAT AAGAACGTGCGTTGGGCAAAAGGTCGGCATGAGACATATCTCTGCTTTGTGGTGAAGAGGAGGGTGGGACCGGACTCCATGTCCTTCGATTTTGGACACCTCCGCAAT------------------CGAGCT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCTACCTG------GGGGCCCTGTGTCCTGGACTGTG GGGCTACGGGGACACCGGAGAC---AGGAGGCTCAGTTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGCGCCAACTGCTCCTTCAGACTGGCCCAGTTCCTCCAAAGGACG---------CCCA ACTTCCGCCTCAGGCTCTTTGTCTCCCGTCTGTACTTCTGTGACATGGAGGACAGCAGTGAGAGGGACGGCCTGAGGTTGCTGAAAAACGCAGGGGTGCAGATCACCGTCATGAGCTACAAAGACTACTTCTATTGCTGGCAGACTTTTGTGGCTCACAGAAAGAGCAGTTTCAAGGCCTGGGATGGGCTGCACCAAAACACTGTTCGCTTGGCCCGG---------TTA---CTCAACCGCATCCTCCAG CCTTGTGAGGCAGAGGATTTGCGGGATGCGTTCAAACTTCTCGGGTTT------------------TGA Lamprogrammus_exutus ATGATTGCAAAACTA---------GACAGTGTGCTTTTGCCCCGCAAAAAGTTCATCTTCCATTAC AAGAACATGCGCTGGGCTAAGGGTCGGCACGAGACATACCTCTGCTTTGTAGTGAAGAGACGAGTGGGTCCAGACTCCCTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCAAT GGCTGCCATGTAGAGCTACTGTTCCTGCGCTACCTG------GGAGCTCTATGCCCTGGACTGTG GGGGTGTGGAGGTTCTGGTGAG---AGGAGACTCAGTTACTCCATCACCTGGTTCTGCTCTTG GTCCCCCTGTGCCAACTGCTCCCAGAGACTATCCCAATTCCTCAGCCAGACA---------CCCAA CCTTCGCCTCAGGATCTTTGTCTCTCGCCTCTACTTCTGTGACATGGAGAACAGCCGTGAGAGAGAGGGCCTGAGGATGCTGAAAAATGCTGGTGTGCAAATCACAGTCATGAGCTACAAAGACTTTTTCTATTGCTGGCAAACCTTTGTGGCTTGTGGGAAAAGCAAATTCAAGGCCTGGGATGAGCTGCACCGAAACTCTGTTCGCCTCACCAGG---------AAA---CTGAACCGCATCCTCCAGC CATGGGAGACAGAAGATTTAAGAGATGCATTCAGACTTCTTGGATTT------------------TGA Lesueurigobius_cf_sanzoi ATGATTACCAAGCTA---------GACAGTGTACTTTTACCAAAGAAGAAGTTTATCTTCCATTAC AAGAACGTGCGCTGGGCGAAGGGTCGGCATGAGACGTACCTCTGCTTTGTGGTCAAGAGGCGCGTGGGGCCAAATTCTATGTCCTTTGACTTTGGACATCTTCGCAAT------------------CGCAGC GGCTGCCATGTGGAGATTCTGTTCCTGCGTTACCTT------GGTGCTCTGTGCCCTGGACTCTG GGGGGCTGGAGGCTCGGAGGAG---AGGCGACTGAGTTACTCCATCACTTGGTTCTGCTCCT GGTCTCCATGCGCCAACTGCTCCACGAAACTGTCGCAGTTCCTCGCCAAAACC---------CCAA ACTTGCGTCTGCGGATATTTGTCTCACGCCTTTACTTCTGCGACCTGGAGGACAGCATAGAACGAGAGGGTCTGAGGATGCTAAAGAGAGCAGGCGTGCAGTTAACGGTCATGAAATACAAAGACTACTTTTACTGCTGGCACACGTTTGTGGCTCGAAACCAAAGCAACTTCAAGGCCTGGGAAGAGCTTCACCAAAACTCAGTGCGACTGACCAGG---------AAA---CTCAGTCGCATCCTTCA GCCATGTGAGACAGAGGATTTAAGAGATGCCTTCAGACTTCTTGGTTTG------------------TGA Lota_lota ATGATAAGTAAGCTA---------GACAGTGTGCTCTTAGCCCAGAAGAAATTCATATACAATTAC AAGAACATAAGATGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGGTGCGGAGGCGACAGAAAC---CGAAGACTCAGCTACTCGGTCACCTGGTTTTGCTCC TGGTCTCCCTGTGCCAACTGTGCGGCTACACTGGCCCGGTTCCTGAGGCAGACG---------CCC AACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATATAGAGGGCTTGAGGGACCTGAGGAGAGCCGGGGTCCAGGTCAAAGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCATGGGAAGGGCTGCATACCAATTCGGTCCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTTTTGGACTGCTAACC------------TGA Macrourus_berglax ATGATTAGTAAGCTT---------GACAGCATACTCTTGGCCCAGAAGAAATTCAAGTACAATTAC AATAACATGCGATGGGCAAAGGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTCGGACCCAATTCACTGTCCTTTGACTTCGGACACCTACGCAAT------------------CGTGC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCGCTGTGCCCGGGCCTGT GGGGCTTTGGAGGGGCAGAAAAC---ATAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGCGCCAACTGTGCGGCCACACTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGAACTGGCGGACAGTCCGCACTCAGAGGGCTTGAGGGAGCTGAGGAGAGCAGGGGTCCAGGTCAACGTTATGACCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCTCATCCTCCAG CCATGTGAAACAGAAGATTTAAGAGACGCTTTCAGACTTATTGGCCTGTTAACC------------TGA Malacocephalus_occidentalis ATGATTAGTAAGCTC---------GACAGCGTGCTCTTGGCCCAGAAGAAATTCATATACAATTAC AAGAACATACGCTGGGCAAAGGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCAATTCACTGTCCTTCGACTTCGGACACCTACGCAAC------------------CGCAC TGGCTGCCATGTAGAGCTGCTGTTTCTGAGCTACTTG------GGGGCGCTGTGCCCGGGCCTGT GGGGCTGTGGAGGTGCAGATAAC---AGAAGACTCAACTACTCGGTCACCTGGTTCTGCTCC TGGTCTCCCTGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCC AACCTGCGCCTCAGGATCTTTGTGGCTCGCCTCTACTTCTGCGACCTGGACGACAGTCCACACACAGAGGGCTTAAGGGAGCTGAGGAGAGCAGGGGTCCAGTTCACCGTAATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGGCTGTTATCC------------TGA Melanogrammus_aeglefinus ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAGGGCCGCAACGAGACCTATCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTCGACTTTGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCACTGTGCCCAGGCCTCT GGGGCTGTGCAGGCGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGACCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGTCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGATCTAAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Melanonus_zugmayeri ATGATTAGTAACCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATGTACAATTAC AAGAACATGCATTGGGCAAAAGGCCGCAACGCGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCGATTCCCTGTCTTTCGACTTCGGACACCTACACAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTTCTCAGCCACCTG------GGGGCACTGTGCCCAGGCCTGT GGGG?TGCGGAGGCGACAAAAAC---AGAAGACTCAGCTATTCGGTTACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGCTTTCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCGTCTACTTCTGTGAACAGGAGGACAGTCCGCATATAGAGGGCTTGAGGGATCTGAGGAGAGCAGGGGTCCAGGTCACCGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGACCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGACTGTTAACC------------TGA Merlangius_merlangus ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATCTACAATTAC AAGAACATGCGGTGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTTTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCACTGTGCCCAGGCCTCT GGGGCTGCGCAGGCGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGAGCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGTCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Merluccius_merluccius ATGATTAGTAAGCTC---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATGTACAATTAC AAGAACATGCGCTGGGCAAAAGGCCGCAACCAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCGATTCCCTGTCTTTCGACTTCGGACACCTACACAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCCACCTA------GGGGCGCTGTGCCCGGGTCTG TGGGGGTGCGGAGGTGACGAAAAC---CGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTC CTGGTCTCCCTGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGACTCACG---------CC CAACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACGTGGAGGACAGTCCGCACAGGGAGGGCTTGAGGAACCTGAGGAGAGCAGGGGTCCTGGTCAACGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------ACA---CTAAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGACGCTTTCAGACTTATTGGGCTGTTAACC------------TGA Merluccius_polli ATGATTAGTAAGCTC---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATGTACAATTAC AAGAACATGCGCTGGGCAAAAGGCCGCAACCAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCGATTCCCTGTCTTTCGACTTCGGACACCTACACAAT------------------CGCAC AGGCTGCCACGCAGAGCTGCTGTTCCTGAGCCACCTA------GGGGCGCTGTGCCCGGGTCTG TGGGGGTGCGGAGGTGACGAAAAC---CGAAGACTCAGCTACTCTGTCACCTGGTTCTGCTCC TGGTCTCCCTGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGACTCACG---------CCC AACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACGTGGAGGACAGTCCGCACAGGGAGGGCTTGAGGAACCTGAGGAGAGCAGGGGTCCTGGTCAACGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------ACA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGACGCTTTCAGACTTATTGGGCTGTTAACC------------TGA Molva_molva ATGATTAGTAAGCTA---------GACAGTGTGCTCTTAGCCCAGAAGAAATTCATATACAACTAC AAGAACATGCGATGGGCAAAAGGCCGCAATGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTCGGACCTGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGGTGCGGAGGCGACACTAAC---CGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCACACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATATAGAGGGCTTAAGGGACCTGAGGAGAGCAGGGGTCCAGGTCAAAGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAAGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTCCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTTTTGGACTGTTAACC------------TGA Monocentris_japonica ATGATTACAAAACTA---------GACAGTGTGCTTTTGGCGCAGAAAAAGTTCATCTACCATTAT AAGAACATGCGCTGGGCAAGGGGTCGGCATGAGACATACCTCTGCTTTGTAGTGAAGAGGAGAGTGGGACCAGACTCCCTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCTC TGGCTGCCATGTAGAGCTGCTGTTCCTGCGCCACCTG------GGAGCCTTGTGCCCTGGACTGT GGGGGTATGGAGGCACTGGTGAG---AGGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCT GGTCTCCCTGCGCTGACTGCTCCTTTAGATTGGTCCAGTTCCTCGGCCGGACG---------CCCAA CCTCCGCCTCAGGATCTTCGTCTCTCGCCTCTACTTCTGTGACGTGGAGGACAGCCGCGAGAGACAGGGCCTGAGAATGCTGAAAAAAGCCGGCGTGCAAATCACTGTCATGAGCTACAAAGACTACTTCTATTGCTGGCAGACCTTCGTGGCTCACAGACAGAGCAGTTTCAAGGCCTGGGATGAGCTGCACCAAAACTCTGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCAG CCTTGTGAGACAGAAGATTTAAGAGATGCGTTCAAGCTTCTTGGGTTG------------------TGA Mora_moro ATGATTAGTACACTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATGTACAATTAC AAGAACATGCGTTGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCCGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCACTGTGCCCAGGCCTGT GGGGGTGCGGAGGCGATGAAAAC---AGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCC TGGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCC AACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTATTTCTGTGACCTGGAGGACAGTCCGCATATAGAGGGCTTGAGGGACCTGAGGAGAGCAGGGGTGCAGGTCACTGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCC AGCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGGCTGTTAACC------------TGA Muraenolepis_marmoratus ATGATTAGCAAACTA---------GACAGTGTGCTCTTGGGCCAGAAGAAATTCATATACAATTAC AAGAACATGCGTTGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTCGTGGTGAAGAGAAGGCTCGGACCCGATTCCATGTCTTTCGACTTCGGGCACCTACGCAAT------------------CGCGC AGGCTGCCACGTGGAGCTGCTGTTTCTCAGCCACCTG------GGGGCGCTGTGCCCGGGTCTGT GGGGTTGCGGAGGCGACGAGAAC---AGACGGCTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCCCCCTGTGCCAACTGTGCCGCCACGCTGGCCCGGCTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCCCGCCTGTACTTCTGTGACCTGGAGGGCAGTCCGCACTCAGAGGGCCTGAGGGACCTGAGGAGGGCCGGGGTCCAGGTCAACGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCGCACAGGGTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCCA GCCACGCGAAACAGACGATTTAAGAGATGCCTTCAGACTTATTGGTCTGTTAACC------------TAA Myoxocephalus_scorpius ATGATTACAAAGCTA---------GACAGTGTGCTATTGCAGCAAAAAAAGTTCATCTACCATTAC AAGAACATGCGCTGGGCAAGGGGCCGACATGAGACTTACCTCTGCTTTGTAGTGAAGAGGCGAGTGGGGCCAGACTCCTTATCCTTTGACTTTGGACACCTCCGCAAT------------------CGCACT GGCTGCCATGTAGAGCTGTTGTTCCTACGCTACCTG------GGAGCCTTGTGCCCTGGTTTGTG GGGTTACGGAGGCACTGGAGAG---AAGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGCATAAACTGCTCCATCAGTTTGTCCCAGTTCCTCAACCGGACG---------CCCAAC CTTCGCCTCAGGATCTTTGTCTCTCGTCTTTACTTCTGTGACAAGGAGAACAGCCGGGAAAGAGATGGCCTGAGAATGCTGAAAAATGCTGGCGTGCAGATCACAGTCATGAGTTACAAAGACTTCTTCTATTGCTGGCAGACATTTGTGGATCGCAAGAAAAGCAACTTCAAGGCCTGGGAAGAGCTGCACCAGAACTCTGTTCGCCTTGCCAGA---------AAA---CTCAACCGCATCCTCCAGC CTTGTGAAGCAGAAGATTTAAGGGATGCCTTCAAGCTTCTTGGACTG------------------TGA Myripristis_jacobus ATGATTACAAAGCTA---------GACAGCATGCTTTTGGCCAAGAAAAAGTTCATTTACCATTAT AAGAACATGCGCTGGGCTAAAGGTCGGCATGAGACATACCTGTGCTTTGTAGTGAAGAGACGAGTGGGGCCAGACTCCATGTCCTTTGACTTTGGACATCTCCGCAAT------------------CGTGCT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCTACCTG------GGAGCGCTTTGCCCTGGACTGTG GGGGTGTGGAGGCAACACTGAG---AAGAAGCTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGCGCCGACTGCTCTTTCAGACTGGCCCAGTTCCTCAACCGGACG---------CCCAA CCTCCGCCTCAGGATCTTTGTCTCTCGCCTCTATTTCTGCGACCTGGAGGACAGCCGTGAGAGAGAGGGCCTGAGGATGCTGAAAAAAGCCGGCGTGCAAATCACTGTTATGAGTTACAAAGATTACTTCTATTGCTGGCAGACATTTGTGGCACATAGAATGAGCAGCTTCAAGGCTTGGGATGGGCTGCACCAAAACTATGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCAGG CTAGTGAGACAGAAGATTTAAGAGATGCATTCAAGCTTCTTGGATTG------------------TGA Neoniphon_sammara ATGATTACAAAGCTA---------GACAGTGTGCTTTTGGCCAAGAAAAAGTTCATCTACCATTAT AAGAACTTGCGCTGGGCAAAAGGCCGGCATGAGACATACCTCTGCTTTGTCGTGAAGAGGCGGGTGGGGCCAGACTCCATTGCCTTCGACTTTGGACACCTCCGCAAT------------------CGTGCT GGCTGCCATGTAGAGCTGCTATTCCTTCGCTACCTG------GGAGCCTTGTGCCCTGGACTGTG GGGGTATGGAGGAACTGGGGAG---AGGAAGCTGAGTTACTCCATCACGTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGCTCCTTCAGACTCGCCCAGTTCCTCAACCGGACG---------CCCA ACCTCCGCCTCAGGATCTTTGTCTCTCGCCTCTATTTCTGTGACGTGGAGGACAGCCGTGAGAGAGAGGGCCTGAGAATGCTGAAAAATGCCGGCGTGCACATCACTGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACATTTGTGGCTCGTAAAACGAGCAGCTTCAAGGCTTGGGATGGGCTGCACCAAAACTATGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCA GCCTTGTGACACAGAAGATTTAAGAGATGCATTCAGGCTTCTTGGATTG------------------TGA Oreochromis_niloticus ATGATTGCAAAGCTA---------GACAGTATGCTTTTGCCCAGAAAAAAGTTCCTCTATCATTAC AAGAATGTGCGCTGGGCGAGGGGCCGGAATGAAACATACCTCTGTTTTGTAGTAAAAAGACGAGTAGGGCCTGACTCCTTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCAAT GGTTGCCACGTTGAGCTGCTGTTCCTGCGCCAACTT------GGTACATTATGCCCTGGCCTGTC TGGGTATGGATTTCATGGGGAG---AGGAGGGTCAGCTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGTGCAAACTGCTCTTCCAGACTGGCCCAGTTCCTCAAACAGACA---------CCCAA CCTTCGCCTCAGGATCTTTGTCTCTCGTCTTTACTTCTGTGACATGGAGGACAGTCGTGAAAGAGAGGGTCTCAGGCTGCTTAAAAAAGTCGGCGTGCACATCACAGTCATGAGTTACAAAGACTTCTTCTACTGCTGGGAGAATTTTGTGGCC---CAGCAAAGCAAATTTAAGGCCTGGGAAGG TCTGCATCAAAACACAGTACGCCTGGCCAGA---------AAA---CTCAACCGCATCCTCCAGCCC TGTGACACAGAAGATTTAAGAGATGCCTTCAAGCTTCTTGGACTG------------------TAA Oryzias_latipes ATGATTACTAAGCTC---------GACAGTGTGCTTCTTCCCAAAAAAAAGTTCATCTATCATTAC AAGAACATGCGCTGGGCAAGAGGCAGACATGAAACATACCTCTGCTTTGTGGTGAAAAGGAGAGTGGGCCCAGAATCTCTGTCCTTTGACTTCGGACATCTCCGCAAT------------------CGTAA TGGCTGCCATGTGGAGCTGTTGTTCCTGCGCCACCTG------AGTGCATTGTGCCCTGGTTTAT GGGGATATGGAGCAACCGGACAG---GGAAGGGTCAGCTACTCCATCACCTGGTTTTGCTCTT GGTCTCCTTGTGCTAACTGCTCTTTCAGACTAGCACAGTTTCTCAGCCAGACC---------CCCAA CCTGCGCCTCAGGATTTTCGTCTCCCGTCTGTATTTCTGTGACTTGGAGGACAGCCGTGAAAGAGAAGGTTTAAGGATGCTGAAAAAAGTTGGCGTGCACATCACAGTCATGAGTTACAAAGATTACTTCTATTGCTGGCAGACCTTTGTGGCTCGGAAACAAAGCAAATTCAAACCCTGGGATGGGCTGCACCAAAACTCTGTTCGGCTTTCAAGA---------AAA---CTCAACCGCATCCTACAG CCATGTGAGACAGAAGATTTCAGAGATGCATTCAAGCTTCTTGGACTG------------------TGA Osmerus_eperlanus ATGATCAGTACGCTA---------GACGGCGTGCTTCTGGCCCAGAAGAAGTTCATCTACCACTAC AAGAACATGCGCTGGGCCAGAGGTCGACACGAGACCTACCTGTGCTTTGTGATCAAGAGGAGGGTTGGGCCCGACTCGCTCTCCTTTGACTTCGGACACCTGCGCAAT------------------CGCACC GGCTGCCATGTAGAGCTGCTGTTTCTACGCCACCTG------GGGGCTCTGTGTCCCGGCCTGTG GGGTACGGGTGGTGCCGGTGGTGGGGTGAGGTTGAGCTACTCCATCACCTGGTTCTGCTCCTGGTCTCCCTGCTCCGCCTGCTCCCACAGGCTGTCTGACTTCCTCAGCCGGACC---------CCCA ACCTCCGCCTCCGGATCTTCGTGTCTCGTCTCTACTTCTGCGACCCGGAAGACAGCCTGGAGAGGGAGGGGCTCCGTATGCTGAAGAGAGCCGGAGTAAACATCACTGTCATGAGTTATAAAGACTATTTCTACTGCTGGGAGACTTTTGTAGCTCGCAGAAAGACAGGCTTCAAGGCCTGGGACGGGCTTCACCACAACTCGGTTCGCCTGGCCAGG---------AAG---CTCTACCGTATCCTACA GCCTTGTGAGACAGAAGATCTGAGAGATGCTTTCACGCTGCTGGGACTG------------------TGA Parablennius_parvicornis ATGATTGCCAAGCTC---------GACAGTATGCTCCTGCCCAGAAAAAAGTTCATCTATCATTAC AAGAACATGCGCTGGGCGAAGGGTCGGCATGAGACTTACCTCTGCTTCGTGGTGAAGCGGCGACTGGGCCCAGACTCTTTGTCCTTTGACTTCGGGCATCTCCGAAAT------------------CGCAAT GGTTGCCATGTAGAGTTGCTGTTCCTGCGCCACCTG------GGGACTTTGTGCCCTGGTCTGTC GGGGTACGGAGTACATGGAGAA---AAAAGGCTTAGCTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGTTCCAACTGTTCTCACCGACTAGCCCAGTTCCTGAGCCGAACG---------CCCAA CATTCGACTCAGAATCTTTGTCTCCCGCCTGTACTTCTGCGACTTGGAGGACAGCCGCGAGAGAGAGGGTCTCCGGCTGCTGAAAAAAACTGGCGTGCATATCACGGTCATGAGCTACAAAGATTATTTCTATTGCTGGCAAACTTTTGTGGCAAGTAATCAGAGCAGGTTTAAGCCTTGGGATGAGCTGCAGCGAAACTCCATCCGCCTCACCAGA---------AAA---CTCAACCGCATCCTCCAGC CCTGCGAAACAGAAGATTTAAGAGATGCCTTCAAGCTTCTTGGACTC------------------TGA Parasudis_fraserbrunneri ATGATTACTAATCTA---------GACAGTGTGCTTCTGGCCCAGAAGAAGTTCATCTACCATTAC AAGAACATGCGGTGGGCAAGGGGCCGGCATGAGACTTATCTCTGCTTTGTAGTGAAGAGAAGGTTGGGTCCAGACTCCTTGTCCTTCGACTTTGGACACCTTCGCAAT------------------CGCTCT GGCTGCCATGTAGAGCTGCTTTTCCTGCGTCACCTG------GGCGCCCTTTGCCCTGGCCTGTG GGGATATGGAGGAGAG---------AAGAGGCTGAGCTACTCTGTCACCTGGTTCTGCTCCTGGTC GCCCTGCGCCGACTGCTCCACCAGACTGTCCCAGTTCCTCAGCAGGACG---------CCCAACCT CCGCCTGAGGATCTTCGTCTCGCGCCTCTACTTCTGCGACCTGGAGGACAGCCTCGCAAGAGAGGGCCTGAGGACACTGAAGAGAGTCGGCGTGCAGGTCACTGTCATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTGGCTCGCAGACAGAGCAGCTTCAAGGCTTGGGATGGGCTGCAGCAGAACTCTGTCCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCAGCC TTGTGAGACAGAAGACTTACGAGATGCATTCAAGCTTCTTGGACTG------------------TGA Perca_fluviatilis ATGATTACAAAGCTA---------GACAGTGTGCTTTTGCCCCGAAAAAAGTTCATCTACCATTAC AAGAACATGCGCTGGGCAAGGGGTCGCCATGAGACATATCTCTGCTTTGTAGTGAAGAGGCGAGTGGGGCCAGACTCCTTATCCTTTGACTTTGGACACCTCCGCAAT------------------CGCAAT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCTACATT------GGAGCCTTGTGCCCTGGTTTGTG GGGATGCAGCGGTACTGGAGAG---AGGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCT GGTCTCCTTGTGCCAACTGCTCCATCAGACTGTCCCAGTTCCTCAGCCAGACG---------CCCA ACCTTCGCCTAAGGATTTTCGTCTCTCGCCTTTACTTCTGTGACACGGAGAACAGCCCTGAAAGAGACGGCCTAAGAATGCTGAAAAAAGCTGGCGTGCAGATCACAGTCATGAGTTACAAAGACTTCTTTTATTGCTGGCAGACCTTTGTGGATCGTAAGCAAAGCAACTTCAAGGCCTGGGAAGAGCTGCACTCAAACTCTGTTCGCCTTTCCAGA---------AAA---CTCAACCGCATCCTCCAGC CTTTTGAAACAGAAGATTTAAGAGATGCCTTCAAGCTTCTTGGACTG------------------TGA Percopsis_transmontana ATGATTACCAAGCTA---------GACAGTGTGCTTCTGGCGCAGAAGAAATTCATCTTCCACTAC AAGAACATGCGCTGGGCAAGGGGTCGCCATGAGACATATCTCTGCTTTGTCATTAAGAGGAGAGTGGGGCCAAACTCCCTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCTCC GGTTGCCATGTAGAGATCCTGTTCCTGCGCCACTTG------GGAGCGCTGTGCCCTGGACTGTG GGGAGAGGGGGGTACTGGTGAG---AGAAGATTAAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGTGCCAACTGCTCCCTCAGACTGGCCCAGATCCTCAGACAGCTG---------CCCAA CCTCCGCCTGAGGATCTTTGTGTCCCGCCTCTACTTCTGTGACCTGGAGGACAGCAAAGAGAGAGATGGCCTCAGAATGCTGAAGAACGTGGGTGTGCAGATCACCGTCATGAGCTACAAAGACTATTTCTATTGCTGGCAGACCTTTGTAGCTCACAGAAAGAGTAACTTCAAAGCCTGGGACGGGCTGCACCAAAACTCTGTTCGCCTGGCTCGG---------AAA---CTCAACCGCATCCTCCAG CCTTGTGAGATAGAAGATTTAAGAGATGCCTTCAAACTTCTTGGGTTT------------------TGA Phycis_blennoides ATGATTAGTAAGCTA---------GACAGTGTGCTCTTAGCCCAGAAGAAATTCATATACAATTAC AAGAACATACGATGGGCAAAAGGCCGCAACGAGACCTACCTCTGCTTTGTAGTGAAGAGAAGGCTCGGACCCAATTCCCTGTCCTTCGACTTCGGTCACCTACGCAAT------------------CGCGC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGGTGCGTGGATGACAGCAAC---AGGAGACTGAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTACGGATGACA---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGACAGTCCGCATATTGAGGGCTTGAGGCACCTGAGGAGAGCAGGGGTTGAGGTCAAAGTTATGAGCTACAAAGACTACTTCTACTGTTGGCAGACCTTCGTAGCTCACAGGCTGAGTCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCAG CCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTTTTGGACTGTTAACC------------TGA Phycis_phycis ATGATTAGTAAGCTA---------GACAGTGTGCTCTTAGCCCAGAAGAAATTCCTATACAATTAC AAGAACATACGATGGGCAAAAGGCCGCAACGAGACCTTCCTCTGCTTTGTAGTGAAGAGAAGGCTCGGACCCAATTCCTTGTCCTTCGACTTCGGTCACCTACGCAAT------------------CGCGC TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGGTGCGTAGATGACAGCAAC---AGGAGACTGAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCATGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTCAGGATGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAAGACAGTCCGCATATTGAGGGCTTGAGGCACTTGAGGAGAGCGGGGGTCGAGGTCAAAGTTATGAGCTATAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGTCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTTTTGGACTGTTAACC------------TGA Poecilia_formosa ATGATTACAAAGCTA---------GACAGGGCACTATTACCCAGAAAAAAATTCATCTATCATTAC AAGAACTTGCGCTGGGCAAGAGGTCGATGTGAGACGTACCTCTGTTTTGTGGTGAAGAAGCGAGTGGGACCAGACTCCCTGTCCTTTGACTTTGGGCATCTCCGCAAC------------------CGCAAC AACTGCCATGTGGAGCTGCTGTTCCTGCGCCACCTG------GGAGCGTTGTGCCCTGGCCTGTG GGGTTATGGAGTCACTGGTGAA---AGAAAAGTCAGCTACTCTGTCACCTGGTTTTGCTCCTG GTCTCCCTGTGCAAACTGCTCCATCCGACTGGCTCAGTTCCTCCACCAGACC---------CCCAA CCTCCGCCTCAGGATCTTTGTATCCCGGCTTTATTTCTGCGACTTGGAGGACAGCCGTGAAAGAGAGGGACTTAGAATACTGAAAAAAGCTGGCGTGCACATCACAGTCATGAGTTACAAAGATTACTTTTACTGCTGGCAGACCTTTGTGGCAAAAAGCCAAAGCAAGTTCAAGCCGTGGGATGGGCTGCACCAAAACTATATCCGGCTGTCAAGG---------AAA---CTCAACCGCATTCTTCAG CCATGTGAGACAGAAGATTTAAGAGATGCCTTCAGGCTTCTTGGACTG------------------TGA Pollachius_virens ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCGCTGTGCCCAGGCCTCT GGGGCTGCGCAGACGACAGAAAC---CGAAGACTAATTTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGTCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCGGACTTTTTGGACTGTTAACT------------TGA Polymixia_japonica ATGATTACTAAACTA---------GACAGTGTGCTTTTGGCCCAGAAGAAATTCATCTACCATTAT AAGAACATGCGCTGGGCGAAGGGTCGACACGAGACGTATCTCTGCTTTGTAGTCAAGAGGAGGGTGGGACCGGACTCCATGTCCTTTGATTTTGGACACCTACGCAAT------------------CGCTCT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCCACCTG------GGGGCCTTGTGCCCTGGACTGTG GGGATACGGAGGTACTGGTGAG---AAGAGGCTCAGTTACTCCGTCACCTGGTTCTGCTCCTG GTCGCCCTGCTCCAACTGCTCCTACAGACTGGCCCAGTTCCTCAGCCAGACG---------CCCAA CCTCCGCCTCAGGATCTTCGTCTCTCGACTTTACTTCTGCGACCTGGAGGACAGCCGGGAGCGAGACGGCCTCAGAATGCTCAAAAGGGCTGGAGTGCAAATCACAGTCATGACCTACAAAGACTACTTCTATTGCTGGCAGACCTTTGTGGCTCACAGAACAAGCAAGTTCAAGGCCTGGGATGAGCTGCACCGGAACTCTGTCCGCCTGTCCAGG---------ATA---CTCAACCGCATCCTCCAGC CTTGTGAGACAGAAGATTTAAGAGATGCCTTCAGACTTCTTGGGTTG------------------TGA Pseudochromis_fuscus ATGATTGCAAAGCTT---------GACAGTGTGCTTTTGCCAAAAAAGAAATTCATCTTTCATTAC AAGAACATGCGCTGGGCAAGGGGCCGACATGAGACATACCTCTGCTTTGTGGTGAAAAGGCGAAGGGGCCCAGACTCTCTGTCCTTTGACTTTGGACATCTCCGCAAT------------------CGCAA CGGCTGCCATGTAGAGCTGCTATTCCTACGGTACCTG------GGAGCCTTGTGCCCTGGTCTGT GGGGGTATGGGGCTACTGGGGCG---AGCAGGCTCAGCTACTCCATCACGTGGTTCTGCTCCT GGTCTCCTTGTGCCAACTGCTCTTTCAGACTGGCCCAGTTCCTCAGCCAGACG---------CCCA ATCTTCGCCTCAGGATCTTCGTCTCTCGCCTTTACTTTTGTGACATGGAGGACAGCCGTGAAAGAGAGGGTCTAAGGCAGCTGAAAAAAGCCGGAGTGCACATCACAGTCATGAGTTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTGGCTCGTAATCAAAGCAAATTCAAGCCCTGGGATGAATTGCACCAAAACTCTGTCCGCCTGTCCAGA---------AAA---CTCAACCGCATCCTCCA GCCTTGTGAGACAGAAGATTTAAGAGATGCCTTCAAGCTTCTTGGACTG------------------TGA Rondeletia_loricata ATGATTACAAAACTA---------GACAGTGTGCTTTTGGCCAAGAAAAAGTTCATCTACCATTAT AAGAACATGCGCTGGGCAAGGGGTCGGCATGAGACATACCTCTGCTTTGTAGTGAAGAGGCGAGTGGGGCCAGACTCCCTGTCCTTCGACTTTGGACACCTCCGCAAC------------------CGCACT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCCACCTG------GGAGCCTTGTGCCCTGGACTGTG GGGGCATGGAGGCACTGGAGAG---AGGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGCGCTGACTGCTCCTTCAGACTGGCCCAGTTCCTCGGCCGGATG---------CCCAA CCTCCGCCTCAGGATCTTCGTCTCTCGCCTCTACTTCTGCGACCTGGAGGACAGCCGCGAGAGAGAGGGCCTGAGGTTGCTGAAAAAAGCCGGCGTGCAGATCACTGTCATGAGCTACAAAGACTTCTTCTATTGCTGGCAGACCTTTGTGGCTCATAGAAATTGCAGCTTCAAGGCCTGGGATGAGATGCATCAAAACTCTGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTGCAGC CTTGTGAGACAGAAGATTTAAGAGATGCGTTCAAGCTTCTTGGGTTG------------------TGA Salmo_salar_1 ATGATCAACAAATTT---------GACAGTGTTCTGTTGGCCCAGAAGAAGTTTATCTACCACTAT AAGAACATGCGCTGGGCCAAGGGCCGACACGAAACCTACCTGTGCTTCGTGGTCAAGAGGCGGGTGGGACCAAACTCACTCTCCTTCGACTTTGGACACCTGCGCAAC------------------CGGTC CGGCTGTCATGTTGAGCTGCTGTTCCTGCGCCTCTTGGAAGCAGGCGCCCTGTGTCCAGGCCTGTGGGGTTATGGAGCTCCAGACAGT---GTGGGACTGTGTTACTCTGTCACCTGGTTCTGTTC CTGGTCCCCCTGCTCAGACTGCTCCTACAGGCTGGCCCAGTTCCTCAGCCAGACC---------CC CAACCTCCGCCTCAGGATCTACGTCTCCAGGCTCTACTTCTGTGACCCGGAGGACAGCAGTGCTAGAGAGGGTCTCCGCATGCTGCAGAGAGCCGGGGTGCAGATCACTGTCATGAACTATGAAGACTATTTCTACTGTTGGCAGACCTTTGTGGCTTGCAGACAGCGTGTTTTTAAGGCCTGGGATGGACTGCATCAGAACTCTGTTCAACTGGCTAGG---------AAA---CTTAACGACATCCTCC AGCCTGGAGAGGCAGAAGATTGGGGAGATGCTTTCGAGCTACTTGGACTG------------------TGA Salmo_salar_2 ATGATCAACAAATTT---------GACAGTGTTCTGTTGGCCCAGAAGAAGTTTATCTACCACTAT AAGAACATGCGCTGGGCCAAGGGCCGACACGAAACCTACCTGTGCTTCGTGGTCAAGAGGCGGGGGGGACCAAACTCACTCTCCTTCGACTTTGGACACCTGCGCAAC------------------CGGT CCGGCTGTCATGTTGAGTTGCTGTTCCTGCGCCTCCTGGAAGCAGGCGCCCTGTGTCCAGGCCTGTGGGGTTATGGAGCTCCAGACAGT---GTGGGACTGTGTTACTCTGTCACCTGGTTCTGTT CCTGGTCCCCCTGCTCAGACTGCTCCTACAGGCTGGCCCAGTTCCTCAGCCAGACC---------C CCAACCTCCGCCTCAGGATCTACGTCTCCAGGCTCTACTTCTGTGACCCGGAGGACAGCAGTGCTAGAGAGGGTCTCCGCATGCTGCAGAGAGCCGGGGTGCAGATCACTGTCATGAACTATGAAGACTATTTCTACTGTTGGCAGACTTTTGTAGCTTGCAGACAGCGTGTGTTTAAGGCCTGGGACGGACTGCATCAAAACTCTGTTCAACTGGCCAGG---------AAA---CTTAACGACATCCTCC AGCCTGGTGAGGCAGAAGATTGGGGAGATGCTTTCGAGCTACTTGGACTG------------------TGA Sebastes_norvegicus ATGATTACAAAGCTA---------GACAGTGTGCTTTTGCCTCGAAAAAAGTTCATCTTCCATTAC AAGAACATGCGCTGGGCAAGAGGCCGGCATGAGACATACCTCTGCTTCGTAGTGAAGAGGCGAGTGGGGCCAGACTCCTTAACCTTTGACTTTGGACACCTCCGCAAT------------------CGCAA TGGCTGCCATGTAGAGCTGCTGTTCATGCGCTACCTG------GGAGCCTTGTGCCCTGGTTTGT GGGGGCAGGGAGTCCCCGGAGAG---AAGAGGCTCAGTTACTCCATCACCTGGTTTTGCTCCT GGTCTCCCTGCGTCAACTGCTCCGTCACACTGTCCCAGTTCCTCAGCAAAACG---------CCCA ACCTTCGCCTCAGGATCTTCGTCTCTCGCCTTTACTTCTGTGACATGGAGAACAGCCGTGAAAGAGATGGACTAAGAATGCTGAAAAAAGCTGGCGTGCAGATCTCAGTCATGAGTTACAAAGACTACTTCTATTGCTGGCAGACCTTTGTGGATCGGAAGCAGAGCAAGTTCAAGGCCTGGGATGAGATGCACCAAAACTCTGTTCGCCTTACCAGA---------AAA---CTCAGCCGCATCCTCCA GCCTAGTGAAACAGAAGATTTAAGGGATGCCTTCAAGCTTCTTGGACTG------------------TGA Selene_dorsalis ATGATTACTAAGCTA---------GACAGTGTGCTTTTGCCCCGCAAAAAGTTTATCTTCCATTAC AAGAATGTGCGCTGGGCGAAGGGCCGGCATGAGACATACCTCTGCTTTGTTGTGAAGAGACGAGTGGGCCCAGACTCCATGACTTTTGACTTTGGACACCTCCGCAAT------------------CGTAAT GGCTGCCATGTAGAGATTCTGTTCCTGCGTTACCTT------GGTGCCTTGTGTCCTGGTCTATG GGGTTATGGGGTTGGTGGAGAG---AAGAGACTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCTCCCTGTGCCAACTGCTCCAGCAGGCTGGCCCAGTTCTTAAAGCAGACG---------CCCAA CCTTCGCCTAAGGATCTTCGTTTCACGCCTTTATTTCTGTGACTTGGAGGACAGCCAAGAGAGAGAGGGCCTGAGGATATTGAAAAAAGCTGGAGTGCACATAACAGTCATGACTTACAAAGACTTCTTCTATTGCTGGCAGACCTTTGTGGCTCGTAAACAGAGTAGCTTCAAAGCCTGGGATGAGCTGCACCAAAATTCTGTTCGTCTTGCTAGA---------AAA---CTTCAGCGTATCCTCCAGCC ATGTGAAACAGAAGATTTGAGGGATGCCTTCAAACTTCTTGGACTG------------------TGA Spondyliosoma_cantharus ATGATTACAAAGCTA---------GACAGTGTGCTTTTGCCTAAAAAAAAATTCATCTACCACTAC AAGAATGTGCGCTGGGCAAGGGGCCGACATGAGACTTACCTGTGCTTTGTAGTGAAGAGGCGAGTGGGGCCAGACACCTTAACCTTTGACTTCGGACACCTCCGCAAT------------------CGCAA CGGCATCCATGTCGAGTTGCTGTTCCTGCGCTATCTG------GGAGCCTTGTGCCCTGGTTTGT GGGGGTATGGAGGCACTGGAGAG---AAGAGGCTGAGTTACTCTATCACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGCTCACTCAGACTGTGCCAGTTCCTCAGCCAGACT---------CCCA ACCTTCGCCTTAGGATCTTCGTCTCTCGCCTCTACTTCTGTGACATGGAGGACAGCCGTGAAAGAGAGGGCCTAAGAATGCTGAAAAAAGCCGGCGTGCAGATCACAGTCATGAGTTACAAAGACTTCTTCTATTGCTGGCAGACATTTGTGGCTCGTAGGGCGAGCCAATTCAAGGCCTGGGAAGAGCTGCAACGTAACTCTGTTCGCCTTACCAGA---------AAA---CTGAACCGCATCCTCCA GCCCTGTGAAACAGAAGATTTAAGAGATGCCTTTAAGCTTCTTGGACTG------------------TGA Stylephorus_chordatus ATGATTGCAAAACTA---------GACAGTGTGCTTCTGGCCCGGAATAAATTCATCTACCATTAT AAGAACATGCGCTGGGCGAAAGGGCGCAACGAGACCTACCTCTGCTTTGTAGTGAAGAGAAGGGTTGGACCTGATTCCCTGGCTTTCGACTTTGGACACCTCCGCAAT------------------CGTAC CGGTTGCCACGTAGAGCTCCTGTTCCTGCGCCACCTG------GGGGCCCTGTGCCCTGGACTGT GGGGA---GGTGCTGCTGGTGAT---AAAAGGCTCAGCTATTCAGTCACCTGGTTCTGCTCTTGG TCTCCCTGTGCCAACTGTGCTTCCACGCTGGCCCAATTCCTGAGACAGACG---------CCAAAC CTCCGTCTCAGGCTCTTTGTGGCTCGTCTCTACTTCTGTGACCTGGAGGATAGTCCTGACAGAGAGGGCCTACGGATTTTGAGAAGAGCTGGGGTGCATATCACAGTTATGAGATACAAAGACTACTTCTACTGCTGGCAGACCTTTGTGGCTCACAACCAGAGCCGCTTCAAAGCCTGGGAAGGACTGCACCCAAACTCTGTCCGTTTGTCCAGA---------ACA---TTAAACCGCATCCTCCAGC CTTGTGAAACAGAAGATTTAAGAGATGCTTTCAAACTCCTTGGATTG------------------TAA Symphodus_melops ATGAATACAAAACTC---------GACAGCGTGCTTTTGCCACGAAAGAAGTTCATTTACCATTAC AAGAACGTGCGCTGGGCAAGGGGCCGGCATGAGACATACCTTTGCTTTGTAATCAAGAGACGGGTGGGGCCGGACACCTTAACCTTTGATTTTGGACACCTCCGCAAT------------------CGCAAT GGCTGCCATGTAGAGCTGCTGTTCCTGCGCTACCTG------GGGGCCTTGTGTCCTGGTTTATT GGGGTATGGAGGCGCCGGAGAG---AAGAGGCTCAGCTACTCTATCACCTGGTTCTGCTCCTG GTCTCCATGCTCCAACTGCTCCACAATACTTTGCCAGTTCCTCAGTAAGATG---------CCCAA CCTTCGCCTCCGGCTCTTCGTCTCTCGCCTTTACTTCTGTGACATGGAGGATAGTCGTGAAAGAGAGGGCTTAAGAATGCTGAAAAAAGTCGGGGTGCAGATCACAATCATGAGTTACAAAGATTTCTTCTATTGTTGGCAGAAATTTGTGGCACGTAGGCAAAGCAACTTCAAGGCATGGGAAGAGCTGCACCAGAACTCTGTTCGTCTTTCCAGG---------AAA---CTCAACCGCATCCTACAG CCCTGTGAAACAGAAGACTTGAGAGATGCGTTCAAGCTTCTTGGACTT------------------TGA Takifugu_rubripes ATGATCACCAAGCTA---------GACAGTATGCTTTTGCCAAGAAAGAAGTTCATCTACCATTAC AAGAATGTGCGATGGGCACGGGGCCGGCACGAGACCTACCTCTGCTTTGTGGTGAAGAGGCGAGTGGGCCCAGACACGCTAACCTTTGACTTTGGACACCTCCGCAAT------------------CGCAG TGGCTGCCACGTAGAGCTGCTTTTCCTGCGTTACCTG------GGAGCCTTATGCCCTGGGTTGT GGGGTTACGGAGCCGCCGGAGAG---AAGAGGCTCAGTTACTCCGTTACCTGGTTCTGCTCCT GGTCTCCCTGTGTCAACTGCTCCATCCAACTCTGCCAGTTTCTCAATAACACC---------CCCAA CCTTCGCCTCAGGATCTTTGTCTCTCGCCTTTACTTTTGTGACCTGGAGGACAGCCTTGAAAGAGAAGGCCTGAGGATGCTGACCAAAGCCGGCGTGAGGATCTCAGTGATGAGCTACAAAGACTATTTCTATTGCTGGCAGAAATTTGTGGATTGCAAAAAGAGCAACTTCAAGGCCTGGGAAGAGCTGCATCAAAACTCTGTACGCCTCACCCGC---------AAA---CTCAATCGCATTCTGCAG GCCTGGGATTTAGAAGACTTACGAGATGCTCTTAAGCTCCTCGGATTC------------------TGA Tetraodon_nigroviridis ATGATTACCAAGCTA---------GACAGTATGCTTTTGCCAAGAAAAAAGTTCCTCTACCATTAC AAGAACGTGCGATGGGCGCGGGGCCGACACGAGACCTACCTCTGCTTTGTTGTGAAGCGGAGAGTGGGCCCAGACACGCTAACCTTTGACTTCGGGCACCTCCGCAAT------------------CGCAA CGGTTGCCACGTAGAGCTGCTCTTCCTGCGCTACCTG------GGGGCCCTGTGCCCGGGTTTGT GGGGTTATGGCGCTGCCGGGGAG---AAGAGGCTCAGTTACTCCATCACCTGGTTCTGCTCCT GGTCCCCCTGCGCCAACTGCTCCATCCAACTTTCCCAGTTTCTGAGGAACACG---------CCCA ACCTTCGCCTCAGAATCTTTGTCTCCCGCCTTTACTTCTGTGACATGGAGGACAGCCTTGAACGGGAAGGCCTGAGGATGCTGTCCAGGGCCGGCGTGAGGATTTCAGTGATGAGCTACAAAGACTTTTTCTATTGCTGGCAGAAATTTGTGGATAGCAAAACGAGCAGCTTTAAAGCCTGGGAAGAGCTGCACCAGAACTCTGTACGCCTCACTCGA---------AAA---CTCAACCGCATTCTCCA GAGCTGGGATTTAGAAGATTTACGAGACGCCCTTAAGCTTCTTGGACTC------------------TAA Theragra_chalcogramma ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAAAAATTCATCTACAATTAC AAGAACATGCGATGGGCAAAAGGCCGCAACGAGACCTATCTCTGCTTCGTAGTGAAGAGAAGGCTTGGACCTGATTCCCTCTCTTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGCAGAGCTGCTGTTCCTGAGCTACCTG------GGGGCGCTGTGCCCGGGCCTCT GGGGCTGCGCAGACGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCGCCCTGTGCCAACTGTGCGACCACGCTGACCCGGTTCCTGAGGCAGACA---------CCCA ACCTGCGACTCAGGATCTTCGTGTCTCGCCTCTACTTCTGTGACCTGGAGGGCAGTCCGCATGTAGAGGGCTTGAGGGACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTGATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTTGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTATGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCAGACTTTTTGGACTGTTAACC------------TGA Thunnus_albacares ATGATTACAAAACTA---------GACAGTGTGCTTTTGCCCCGGAAAAAGTTCATCTACCATTAC AAGAACGTGCGCTGGGCAAGAGGACGGCATGAAACATACCTCTGCTTTGTAGTGAAGAGGCGAGTGGGGCCAGACTCTTTATCCTTTGACTTTGGACACCTGCGCAAT------------------CGCAA TGGCTGCCATGTAGAGCTGCTGTTCCTGCGATATCTG------GGAGCCTTGTGCCCTGGTGTGT GGGGGTATGGAAATACTGGACAG------AGGATCAGTTACTCCATCACCTGGTTCTGCTCTTG GTCTCCCTGTGCCAACTGCTCTCGCAGACTGGCCCAGTTCCTCAGCCAGGTA---------CCCAA CGTTCGCCTTAGGATCTTCGTATCACGCCTCTACTTTTGTGACTTGGAGGACAGCCGTGAGAGAGACGGCCTGAGGTTGCTAAAAAACGCCGGCGTGCAGATCACAGTCATGAGTTACAAAGACTTCTTCTACTGCTGGCAGACTTTTGTAGCTCGTAATCAGAGCAAATTCAAGGCCTGGGAAGAGCTGCACCGAAACTCTGTTCGCCTAACAAGA---------ACA---CTCAACCGCATACTCCAGC CCTGTGACATTGATGATTTAAGAGATGCCTTCAAGCTTCTTGGGCTG------------------TGA Trachyrincus_murrayi ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTCATCTACAATTAC AAGAACATGCGTTGGGCAAAAGGCCGCAACGAGACCTACCTATGCTTTGTGGTGAAGAGAAGGCTTGGACCTGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCCT TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCGCTGTGCCCGGGCCTGT GGGGGTGTGGAGGCGACGTAAAC---AGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTCCT GGTCTCCCTGCGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGACAGTCCGCATATAGAGGGCTTGAGGGATCTGAGGAGAGCAGGGGTCCAGGTCACCGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGACTGTTAACC------------TGA Trachyrincus_scabrus ATGATAAGTAAGCTA---------GACAGTGTGCTCTTGGCTCAGAAGAAATTCATCTACAATTAC AAGAACATGCGTTGGGCAAAAGGCCGGAATGAGACCTACCTATGCTTTGTGGTGAAGAGAAGGCTTGGACCTGATTCCCTGTCTTTCGACTTCGGACACCTACGCAAT------------------CGCCT TGGCTGCCACGTAGAGCTGCTGTTTCTGAGCCACCTG------GGGGCACTGTGCCCGGGCCTG TGGGGGTGCGGAGGCGACGAAAAC---AGAAGACTCAGCTACTCGGTCACCTGGTTCTGCTC CTGGTCTCCCTGCGCCAACTGTGCGGCCACACTGGCCCGGTTCCTGAGGCACACG---------CC CAACCTGCGCCTCAGGATCTTCGTGGCTCGCCTCTACTTCTGTGACCTGGAGGACAGTCCGCATATAGAGGGCTTGAGGGATCTGAGGAGAGCAGGGGTCCAGGTCACTGTTATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTCCGTCTGTCCAGA---------AAA---CTAAACCGCATCCTC CAGCCATGTGAAACAGAAGATTTAAGAGATGCTTTCAGACTTATTGGACTGTTAACC------------TGA Trisopterus_minutus ATGATTAGTAAGCTA---------GACAGTGTGCTCTTGGCCCAGAAGAAATTTATATACAATTAC AAGAACCTACGATGGGCAAAAGGACGCAACGAGACCTACCTCTGCTACGTAGTGAAGAGGAGGCTCGGACCTGATTCCCTCTCCTTCGACTTCGGACACCTACGCAAT------------------CGCAC TGGCTGCCACGTAGAGCTGCTGTTCCTCAGCTACCTT------GGGGCACTATGCCCGGGCCTCT GGGGCTGCACCGATGACAGAAAC---CGAAGACTGAGCTACTCCGTCACCTGGTTCTGCTCCT GGTCTCCCTGTGCCAACTGTGCGGCCACGCTGGCCCGGTTCCTGAGGCAGACG---------CCCA ACCTGCGCCTCAGGATCTTCGTCGCCCGCCTCTACTTCTGCGACCTGGAGGGCAGTCCGCACATAGAGGGCTTGAGGCACCTGAGGAGGGCAGGGGTCCAGGTCAAAGTCATGAGCTACAAAGACTACTTCTACTGCTGGCAGACCTTCGTAGCTCACAGGCTGAGCCGCTTCAAGGCCTGGGAAGGGCTGCATACCAATTCTGTGCGTCTGTCAAGA---------AAA---CTAAACCGCATCCTCCA GCCATGTGAAACAGAAGATTTAAGAGATGTTTTCGGACTTTTTGGACTGTTAACC------------TGA Typhlichthys_subterraneus ATGATTAGCAAGCTA---------GACAGTGTCCTTCTGGCGCAGAAGAAATTCATCTTCCACTAT AAGAATATGCGCTGGGCAAGGGGGCGCAATGAGACATATCTCTGCTTTGTCATTAAGAGGAGAGTGGGGCCGGACTCCCTGTCCTTTGACTTTGGACACCTCCGCAAT------------------CGCTCC GGCTGCCATGTAGAGCTGCTGTTCCTGCGCCACTTG------GGGGCGCTGTGCCCTGGCCTGTG GGGACAGGGGGGTACAGGTGAC---AACAGACTCAGTTACTCCATCACCTGGTTCTGCTCCTG GTCCCCCTGTTCCAACTGCTCTCACAGACTGGCCCAGTTCCTCAGCCAGCTG---------CCCAA CCTCCGCCTGAGGATCTTTGTGTCCCGTCTGTACTTCTGTGACCTGGAGGACAGCAGGGAGAGAGAGGGCCTGAGAATGCTGAAGAATGCGGGCGTGCACATAACCGTCATGAGCTACAAAGACTATTACTATTGCTGGCAGACCTTTGTAGCTCGCAGAAAGAGTAAATTCAAAGCATGGGAAGGGCTGCACCAAAACTCTGTTCGCCTGGCCAGG---------AAA---CTCAACCGCATCCTCCAG CCGTGCGAGATAGAAGATTTAAGAGATGCCTTCAAACTTCTTGGGTTT------------------TGA Xiphophorus_maculatus ATGATTACAAAGCTA---------GACAGGGTACTATTACCCAAAAAAAAATTCATCTATCATTAC AAGAACATGCGCTGGGCAAGAGGTCGATGTGAGACATACCTCTGCTTTGTGGTGAAGAAGCGAGTGGGACCAGACTCCCTGTCCTTTGACTTTGGACATCTCCGCAAC------------------CGCAAC AACTGTCATGTGGAGCTGCTGTTCCTGCGCCACCTG------GGAGCGTTGTGCCCTGGCCTGTG GGGTTATGGAGTCACTGGTGAG---AGAAAAGTCAGCTACTCCATCACCTGGTTTTGCTCCTG GTCTCCCTGTGCAAACTGCTCCTTCCGACTGGCTCAGTTCCTCCACCAGACC---------CCCAA CCTCCGCCTCAGGATCTTTGTATCCCGGCTTTATTTCTGTGACTTGGAGGACAGCCGTGAAAGAGAGGGACTTAGAATGCTGAAAAAAGCTGGCGTGCACATCACAGTCATGAGTTACAAAGATTACTTTTACTGCTGGCAGACCTTTGTGGCAAAAAGTCAAAGCAAGTTCAAGCCGTGGGATGGGCTGCACCAAAACTGTATCCGGCTGACAAGG---------AAA---CTCAACCGCATACTTCAG CCATGTGAGACAGAAGATTTAAGAGATGCCTTCAGGCTTCTTGGACTG------------------TGA Zeus_faber ATGATAACTAAACTA---------GACAGTGTGCTTCTGGCTCGGAAGAAATTCATTTACCACTAT AAGAACATGCGCTGGGCAAAAGGCCGCTGTGAGACGTACCTCTGCTTTGTCGTCAAGAGGAGAGTTGGACCCAATTCCCTGTCCTTTGACTTTGGACACCTTCGCAAT------------------CGGACC GGCTGCCATGTAGAGCTCCTGTTTCTACGTCACCTG------GGAGCCTTGTGCCCTGGACTGTG GGGACACGGAGGCCCCTATGGA---GGGCGGCTCAGTTACTCAGTCACCTGGTTCTGCTCGTG GTCTCCCTGCGCCAACTGCTCCTTCAGACTGGCCCAATTCCTCGGGCAGACG---------CCCAA CCTCCGCCTCAGGATCTTTGTCTCCCGCCTCTACTACTGCGACCTTGAAGATAGCCGCGAGAGAGAGGGCTTACGGATCCTGAAAAGAGCCGGAGTCCAAATCACAGTCATGAGCTACAAAGACTACTTCTATTGCTGGCAAACCTTCGTGGCTCACAGACAGACCAGCTTCAAGGCGTGGGATGAGCTGCACCAAAACTCAGTTCGCCTGGCCAGG---------AAA---CTAAACCGCATCCTCCAG CCTTGTGAAACAGAAGATTTAAGAGATGCCTTCAAACTTCTTGGGTTCTTG---------------TGA ; end; begin trees; tree speciestree = (((Astyanax_mexicanus:121.77122741279602,Danio_rerio:121.77122741279602):101.99953637657163,((((((((((((((((Gasterosteus_aculeatus:55.40747769565583,Myoxocephalus_scorpius:55.40747769565583):22.627589007329938,(Sebastes_norvegicus:71.41027871780396,Chaenocephalus_aceratus:71.41027871780396):6.624787975823878):5.948298200333113,Perca_fluviatilis:83.98336487731933):27.234997279977804,((((Takifugu_rubripes:46.743550480651855,Tetraodon_nigroviridis:46.743550480651855):52.31449333152771,Antennarius_striatus:99.05804377441406):4.952094098567969,Spondyliosoma_cantharus:104.0101378944397):3.8940865391969623,Symphodus_melops:107.90422446670532):3.314137740588194):4.0983751179695105,(((Oreochromis_niloticus:90.12568147794833,(Oryzias_latipes:69.76327566986083,(Poecilia_formosa:18.384480726242064,Xiphophorus_maculatus:18.384480726242064):51.37879498329163):20.362405779966025):6.229750216332775,((Chromis_chromis:86.03637034606933,Pseudochromis_fuscus:86.03637034606933):6.271233430540818,Parablennius_parvicornis:92.30760380917813):4.047827894961586):15.657636823177327,((Helostoma_temminckii:64.19328314971924,Anabas_testudineus:64.19328314971924):42.49635722122191,Selene_dorsalis:106.6896403640747):5.32342815576196):3.303668787118795):2.6957501523196754,(Thunnus_albacares:103.80823429222107,Lesueurigobius_cf_sanzoi:103.80823429222107):14.204253155434131):5.149373299789431,Chatrabus_melanurus:123.16186078948975):4.574033951210964,((Lamprogrammus_exutus:66.95888729228973,Carapus_acus:66.95888729228973):23.925274275398266,Brotula_barbata:90.88416159934998):36.85173311395644):9.028980841016761,((Myripristis_jacobus:59.01862996520996,(Holocentrus_rufus:14.652058449554444,Neoniphon_sammara:14.652058449554444):44.366571516036984):70.12873428974152,((Rondeletia_loricata:89.71703486652375,Acanthochaenus_luetkenii:89.71703486652375):23.455124383091928,Beryx_splendens:113.17215923690796):15.97520504798888):7.61751127405762):3.0117428180396644,Monocentris_japonica:139.776618334198):7.268487315320982,Lampris_guttauts:147.04510569152832):3.839686785376074,(((((((Molva_molva:42.47743926963806,(Brosme_brosme:39.03891726341247,(((((Arctogadus_glacialis:5.222854929506778,Boreogadus_saida:5.222854929506778):2.4513389710009097,(Theragra_chalcogramma:3.346329225230217,Gadus_morhua:3.346329225230217):4.327864675396681):5.726030785477162,(Melanogrammus_aeglefinus:10.395505192184448,Merlangius_merlangus:10.395505192184448):3.0047194936364896):4.48665917098522,Pollachius_virens:17.886883866405487):9.021208262825013,(Trisopterus_minutus:22.696635680580137,Gadiculus_argenteus:22.696635680580137):4.211456434738636):12.130825152540204):3.4385220028579297):3.19094077802896,Lota_lota:45.6683800485611):13.098774013638497,(Phycis_blennoides:16.400560005474087,Phycis_phycis:16.400560005474087):42.366594044685364):12.221774325680741,((Merluccius_merluccius:5.7998921918630600,Merluccius_polli:5.7998921924829485):61.73268190526217,(Melanonus_zugmayeri:63.95622381646633,((((Macrourus_berglax:29.67249945344925,Malacocephalus_occidentalis:29.67249945344925):20.17204357004166,Bathygadus_melanobranchus:49.84454303512574):10.645187737723738,(Mora_moro:36.98188234682083,Laemonema_laureysi:36.98188234682083):23.5078484090443):1.939285897175786,(Muraenolepis_marmoratus:56.88453414344788,(Trachyrincus_scabrus:12.07161490740776,Trachyrincus_murrayi:12.07161490740776):44.81291924285889):5.5444825193190255):1.5272070992565432):3.576350340722499):3.4563542779281846):33.47187371263503,Stylephorus_chordatus:104.46080207824707):21.041643343019487,(Zeus_faber:32.85098531341553,Cyttopsis_roseus:32.85098531341553):92.65146017112733):19.170966317129142,(Polymixia_japonica:135.7494994041443,(Percopsis_transmontana:60.18133554153442,Typhlichthys_subterraneus:60.18133554153442):75.56816387424469):8.923912356662754):6.211380692934995):7.560750375115873,Benthosema_glaciale:158.44554285736086):10.299416859668469,(Parasudis_fraserbrunneri:161.78278560620342,Guentherus_altivela:161.78278560077345):6.962174173392896):25.783923850154878,(Osmerus_eperlanus:117.3724450843811,Borostomias_antarcticus:117.3724450843811):77.15643841142654):17.481817657327667,(Salmo_salar_1:0.00662978935994194395,Salmo_salar_2:0.00857699553333491141):212.0107011795044):11.760062609915053):10,Lampetra_tridentata:232); end; begin mrbayes; log start filename=log.out; charset 1st_pos=1-.\3; charset 2nd_pos=2-.\3; charset 3rd_pos=3-.\3; partition by_codon=3:1st_pos,2nd_pos,3rd_pos; set partition=by_codon; lset applyto=(all) nst=6 rates=gamma; unlink revmat=(all) statefreq=(all) shape=(all) ratemultiplier=(all); prset applyto=(all) ratepr=variable; constraint gadiformes = 32 31 6 26 40 70 71 41 34 36 37 20 68 9 4 35 33 55 19 72 11 38 30 53 52; prset applyto=(all) topologypr=constraints(gadiformes); report applyto=(all) ancstates=yes siterates=yes; outgroup 21; showmodel; taxastat; mcmcp samplefreq=5000 printfreq=5000 nruns=24 nchains=10 starttree=current nperts=4 nswaps=3 temp=0.01; mcmcp savebrlens=yes filename=asr_gadiformes_gtr_outgroup_tree ngen=6000000; sump; sumt; End; ########################################################################################################################## ##############################################ProtASR setting and input files############################################# ########################################################################################################################## ProtASR setting file ########################################################################################################################## ##### Settings file for ProtASR 2.0 ##### Ancestral sequence reconstruction of proteins under structurally constrained substitution models ##### Miguel Arenas, David Liberles & Ugo Bastolla ##### (c) 2014-2015 ##### Contact: miguelmmmab@gmail.com ##### ##### Parameters with an "*" are mandatory (must be specified) ##### Text with an "#" is not read. Parameter values must be introduced immediately after the "=" ########################################################################################################################## #################################################### #### Alignment of amino acid sequences and tree #### #################################################### ### Target alignment file with a rooted tree ### # nexus format with a rooted tree, see documentation and examples *NameOfNexusFile=60_teleosts.nex ############################# ### Settings for the ASR ### ############################# # Substitution model: MEANFIELD (requires specification of settings in the following section), Blosum62, cpREV64, Dayhoff, DayhoffDCMUT, G1974a, G1974c, G1974p, G1974v, Grantham, HIVb, HIVw, JTT, JonesDCMUT, LG, Miyata, MtArt, MtMam, MtRev24, MtZoa, RtRev, VT, WAG *SubsModel=MEANFIELD # Consider frequencies from the model (+F) (0: No, 1: Yes) *ModelFreqs=0 # Estimate (0) or fix (1) gamma shape parameter *TypeG=1 # Gamma shape parameter value. Initial or fixed alpha, 0:infinity (constant rate) *AlphaG=0 # Different alphas for genes, introduce a number *Malpha=0 # Estimate (0) or fix (1) rho (correlation parameter) *TypeRho=1 # Rho (correlation parameter). initial or fixed rho, 0:no correlation *RhoCorr=0 ############################################################################################### ### Settings to compute the substitution model based on the protein structure - MEANFIELD - ### ############################################################################################### ### Input files defining the protein ### # PDB file (must be placed in the current directory) *PDBfile=model3.pdb # Chain of the PDB file *CHAIN=A ### Thermodynamic model ### # Temperature *TEMP=0.5 # Configurational entropy per residue (unfolded) *SU1=0.065 # Configurational entropy per residue (misfolded) *SC1=0.065 # Configurational entropy offset (misfolded) *SC0=0.0 # Use up to 1,2,3 moments of misfolding energy? *REM=2 # Contacts map (must be placed in the ProtEvol directory) *FILE_STR=structures.in ### Mean field model ### # Number of substitutions to simulate data (0 by default, not required for ASR) *TMAX=0 # LAMBDA~ NPOP*exp(-DELTA G/TEMP) *LAMBDA_par=0.90 # Optimize LAMBDA? (0: No, 1: Yes, default) OPT_LAMBDA=1 # Target value of DeltaG if OPT_LAMBDA DG_OPT=-1 # Optimization criterion. Allowed: NAT ALL DG *MODEL=ALL ### Mutation model ### # Global matrix. Mean (0) or mean weighted by frequencies (1) *GLOBALMATRIX=0 # Exchangeability. Allowed: MUT, EXCH, FLUX, RATE *EXCHANGE=FLUX # Rate matrix. Allowed: JTT, WAG *MATRIX=JTT # Get nucleotide frequencies from sequence? (0: Use input nucleotide frequencies, 1: Fit nucleotide frequencies from prot sequences, 2: Fit amino acid frequencies from prot sequences) *GET_FREQ=2 ## DNA Parameters for model MUT ## # Frequency for A *fA=0.25 # Frequency for T *fT=0.25 # Frequency for C *fC=0.25 # Frequency for G *fG=0.25 # Transition transversion ratio (Kappa, >1) *TT_RATIO=1.3 # Ratio between 1-nuc and 2-nuc mutations (0-1) *TWONUCMUT=0.25 ########################################################################################################################## ##### Settings file for ProtASR 2.2 ##### Ancestral sequence reconstruction of proteins under structurally constrained substitution models ##### Miguel Arenas & Ugo Bastolla ##### (c) 2014-2018 ##### Contact: miguelmmmab@gmail.com ##### ##### Parameters with an "*" are mandatory (must be specified) ##### Text with an "#" is not read. Parameter values must be introduced immediately after the "=" ########################################################################################################################## #################################################### #### Alignment of amino acid sequences and tree #### #################################################### ### Target alignment file with a rooted tree ### # nexus format with a rooted tree, see documentation and examples *NameOfNexusFile=60_teleosts.nex ############################# ### Settings for the ASR ### ############################# # Substitution model: MEANFIELD (requires specification of settings in the following section), Blosum62, cpREV64, Dayhoff, DayhoffDCMUT, G1974a, G1974c, G1974p, G1974v, Grantham, HIVb, HIVw, JTT, JonesDCMUT, LG, Miyata, MtArt, MtMam, MtRev24, MtZoa, RtRev, VT, WAG *SubsModel=MEANFIELD # Consider frequencies from the model (+F) (0: No, 1: Yes) *ModelFreqs=0 # Estimate (0) or fix (1) gamma shape parameter *TypeG=0 # Gamma shape parameter value. Initial or fixed alpha, 0:infinity (constant rate) *AlphaG=0.5 # Different alphas for genes, introduce a number *Malpha=0 # Estimate (0) or fix (1) rho (correlation parameter) *TypeRho=1 # Rho (correlation parameter). initial or fixed rho, 0:no correlation *RhoCorr=0 ############################################################################################### ### Settings to compute the substitution model based on the protein structure - MEANFIELD - ### ############################################################################################### ### Input files defining the protein ### # PDB file (must be placed in the current directory) *PDBfile=Gadus_morhua.pdb # Chain of the PDB file *CHAIN=A ### Thermodynamic model ### # Temperature *TEMP=0.5 # Configurational entropy per residue (unfolded) *SU1=0.065 # Configurational entropy per residue (misfolded) *SC1=0.065 # Configurational entropy offset (misfolded) *SC0=0.0 # Use up to 1,2,3 moments of misfolding energy? *REM=2 # Coefficient of local interactions *A_LOC=0 # Contacts map (must be placed in the ProtEvol directory) *FILE_STR=structures.in ### Mean field model ### # Number of substitutions to simulate data (0 by default, not required for ASR) *TMAX=0 # LAMBDA~ NPOP*exp(-DELTA G/TEMP) *LAMBDA_par=0.90 # Optimize LAMBDA? (0: No, 1: Yes, default) OPT_LAMBDA=1 # Target value of DeltaG if OPT_LAMBDA DG_OPT=-1 # Optimization criterion. Allowed: NAT ALL DG *MODEL=ALL # WildType model: No (0) or yes (1) *WTmodel=1 ### Mutation model ### # Global matrix. Mean (0) or mean weighted by frequencies (1) *GLOBALMATRIX=0 # Exchangeability. Allowed: MUT, EXCH, FLUX, RATE *EXCHANGE=FLUX # Rate matrix. Allowed: JTT, WAG *MATRIX=JTT # Get nucleotide frequencies from sequence? (0: Use input nucleotide frequencies, 1: Fit nucleotide frequencies from prot sequences, 2: Fit amino acid frequencies from prot sequences) *GET_FREQ=2 # Improve mutation parameters after selection? *REMUT=0 ## DNA Parameters for model MUT ## # Frequency for A *fA=0.25 # Frequency for T *fT=0.25 # Frequency for C *fC=0.25 # Frequency for G *fG=0.25 # CpG transition ratio *kCpG=2 # Transition transversion ratio (Kappa, >1) *TT_RATIO=1.3 # Ratio between 1-nuc and 2-nuc mutations (0-1) *TWONUCMUT=0.25 ########################################################################################################################## ProtASR input file #NEXUS [ Real data set from NCBI PDBtaxa=Gadus_morhua ] Begin data; Dimensions ntax=74 nchar=217; Format datatype=protein gap=- missing=? matchchar=.; Matrix Acanthochaenus_luetkenii MITKLDRVLLAKETFIFHYENMRWAKGRHETYLCFVVKRRVGPDSLSFDFGHLRNRTGCHVE-LLFLRHL--GTLCPGLWGYGGAGE-RRLSYSITWFCSWSPCADCAFRVAQLIGRTPNLRLRIFVSRLYFCDLEDSRERGGLRLLKKAGVQITVMSYKDFFYCWQTFVANGGSSFKAWDEMHQNSVRLASQLNHILQPCDTEDLRDAFKLLGL-- Anabas_testudineus MITKLDSVLLPRKKFIYHYKNVRWARGRHETYLCFVVKRRVGPDSLTFDFGHLRNRNGCHVE-MLFLRYL--GALCPGIWGYGGAGE-KRLSYSITWFCSWSPCANCSLRLTQFLSQTPNLRLRIFVSRLYFCDMEDSREREGLRILKNAGVQITVMTYKDFFYCWQTFVDRKQSSFKAWDELHQNSVRLTRKLYRILQPCEIEDLRDAFKLLGL-- Antennarius_striatus MITKLDSVLLPRKKFIYHYKNMRWARGRCETYLCFVVKRREGPDTLTFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGSGGTGE-KRLSYSITWFCSWSPCANCSIRQCEFLSRTPNLRLRIFVSRLYFCDLEDSREREGLRMLKKAGVQISVMSYKDFFYCWQTFVASKQSSFKAWEELHQNSVRLARKLNRILQPCEAEDLRDAFKLLGL-- Arctogadus_glacialis MISKLDSVLLAQNKFIYNYKNMRWAKGRNETYLCFVMKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCADDRN-RRLSYSVTWFCSWSPCANCATTLTRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRK?NRILQPCETEDLRDVFRLFGLLT Astyanax_mexicanus MTSKLDSILLTQKKFIYHYKNVRWARGRHETYLCFVVKRRIGPNSLSFDFGHLRNRSGCHVE-LLFLRYL--GALCPGLGGLGVDGV--KVGYAVTWFCSWSPCSNCAQRIAHILSQTPSLRLRIFVSRLYFCDNEDSLEREGLRHLLRAGVQITVMTYKDFFYCWQTFVARRESRFKAWDGLHQNSVRLSRKLKRILQPCQTEDLRDVFALLGL-- Bathygadus_melanobranchus MISKLDSVLLAQKKFMYNYKNVRWAKGRHETYLCFVVRRRLGPNSLSFDFGHLRNRTGCHVE-LLFLSHL--GALCPGLWGCVGDDN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEDSPNIEGLRELRRAGVQVIVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRVIGLLS Benthosema_glaciale MITKLDSVLLGQKKFLFHYKNVRWAWGRNETYLCFVVKRRVGPNSLSFDFGHLRNRSSCHAE-LLFLRHL-GGALCPGLWGYGGDGGEGRFNYSVTWFCSWSPCADCSLRLAQFLSRTPNLRLRIFVSRLYFCDAEDSREREGLRTLKRAGVQITVMNYKDYYYCWQTFVAHRQSSFKAWADLHQNSVRLARKLHRILQPCETEDFRDAFKLLGL-- Beryx_splendens MITKLDSVLLAKKKFIYHYKNMRWAKGRHETYLCFVVKRRVGPDSLSFDFGHLRNRAGCHVE-LLFLRHL--GALCPGLWGHGGSGE-RKLSYSITWFCSWSPCADCSFRLAQFLNRTPNLRLRIFVSRLYFCDQEDSRERDGLRLLKKAGVNITVMSYKDFFYCWQTFVANRTSRFKAWDLLHQNSVRLARKLNRILQPYEIEDLRDAFRLLGF-- Boreogadus_saida MIRKLDSVLLAQNKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLHNRTGCHAE-LLFLSYL--GALCPGLWGCADDRN-RRLSYSVTWFCSWSPCANCATTLTRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDPRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYV?LSRKLNRILQPCETEDLRDVFRLFGLLT Borostomias_antarcticus MISKLDSVLLAQKKFLFHYKNVRWARGRHETYLCFVVKRRVGPDSLTFDFGHLRNRTGCHVE-LLFLRHL--GVLCPGLSASGGAGGGRGLNYSITWFCSWSPCFDCSARLAQFLRRTPNLRLRLFVSRLYFCDPEDRHEREGLRALKRAGVHITVMSYKDYFYCWQTFVAHRQRAFKAWEDLQQNSVRLARKLNSILLPCETEDLRDPFRLLGL-- Brosme_brosme MMSKLDSVLLAQKKFIYNYKNLRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCGGDRN-QRLSYSVTWFCSWSPCANCAATLARFLRHTPNLRLRIFVARLYFCDLEGSPHIEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRALNRILQPCETEDLRDPFRLFGLLT Brotula_barbata MIAKLDSVLLPRKKFIYHFKNMRWAKGRHETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGCGNSGQ--RLCYSITLFCSWSPCANCSERLAKFLGRTPNLRLRIFVSRLYFCDMEDSREREGLRMLKNAGVNITVMSYKDYFYCWQTFVARGASNFKAWDGLQENSIRLARKLTHILQPGETEDLRDAFKLLGM-- Carapus_acus MTAKLDRVLLPRKKFLFHYKNVRWAKGRHETYLCFVVKRRVGPDSMSFDFGHLRNRSGCHVE-LLFLRYL--GALCPGLWGYEGSGQ-RRLSYSITWFCSWSPCANCSERLAQFLNRTPNLRLRIFVSRLYFCDLEDSREREGLRTLEKAGVHITIMSYKDYFYCWQTFVACGTSKFKAWDELHQNTTRLKRKLNRILQPCETEDLRDAFKLLGLL- Chaenocephalus_aceratus MITKLDSMLLPRKKFIYHYKNMRWARGRCETYLCFVVKRRVGPDSLTFDFGHLRNRNGCHVE-MLFLRYL--DALCPGLLGCEGTGE-KRLSYSITWFCSWSPCANCSIRLSQFLSQTPNLRLRIFVSRLYFCDMENSPARDGLIMLKKAGVQTSVMSYKDFFYCWHNFVDCKQSKFKPWEDLHQNSVRLARKLKRILQLCETEDLRDAFKLLGL-- Chatrabus_melanurus MITKLDSVLLPRKKFIYHYKNMRWAKGRHETYLCFVVKRRMGPDSLSFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGYGIAGE-RKLSYSVTWFCSWSPCVNCSLRLTQFLMQTPNLRLRIFVSRLYFCDMEDSREREGLRMLKKAGVHITVMSYKDFFYCWQTFVACKESKFKAWEALHQNSVRLARKLNRILQPCETEDFRDAFKLLGL-- Chromis_chromis MITKLDSVLLPQKKFIYHYKNMRWARGRCETYLCFVIKKRAGPDSISFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGYGQ----NRISYSITWFCSWSPCANCSLRLAQFLNQTPNLRLRIFVSRLYFCDMEDSREREGLRILKKAGVNITVMSYKDYFYCWQTFVARRLSKFKPWDGLQQNYVRLSRKLNRILQPCETEDFRDAFRLLGL-- Cyttopsis_roseus MITKLDSVLLARKTFIYHYKNMRWAKGRHETYLCFVVKRRVGPDSLSFDFGHLRNRTGCHVE-LLFLRHL--GALCPGLWGQGGADE-RRLSYSVTWFCSWSPCANCSLRLVQFLGQTPNLRLRIFVSRLYYCDLEDSREREGLRTLKRAGVQITVMSYKDYFYCWQTFVARRQTRFKAWDELHQNSVRLARKLNRILQPCETEDLRDAFKLLGFL- Danio_rerio MICKLDSVLMTQKKFIFHYKNVRWARGRHETYLCFVVKRRIGPDSLSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGLSASSVDGA--RLCYSVTWFCSWSPCSKCAQQLAHFLSQTPNLRLRIFVSRLYFCDEEDSVEREGLRHLKRAGVQISVMTYKDFFYCWQTFVARRERSFKAWDGLHENSVRLVRKLNRILQPCETEDLRDVFALLGL-- Gadiculus_argenteus MISKLDSVLLAQKKFIYNYNNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-VLFLSYL--GALCPGLWGCAGDRS-LRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDVFRLFGLLT Theragra_chalcogramma MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCADDRN-RRLSYSVTWFCSWSPCANCATTLTRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDVFRLFGLLT Gadus_morhua MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCADDRN-RRLSYSVTWFCSWSPCANCATTLTRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDVFRLFGLLT Gasterosteus_aculeatus MIAKLDSVLLPRKKFIYHYTNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGFLGCGDTGG-RRLSYSITWFCSWSPCVNCSISLSQFLSRTPNLRLRIFVSRLYFCDMENSRERDGLRMLKKAGVQVTVMSYKDFFYCWQTFVDRKQSQFKAWKELHQNSVRLSRKLKRILQPCETEDLRDAFKLLGL-- Guentherus_altivela MITKLDSILMAQKKFIFHYKNMRWAKGRNETHLCFVVKRRLGPNSLSFDFGHLRNRTGCHVE-LLFLRHL--GFLCPGLWGYGEPGE-GRLNYSVTWFCSWSPCADCSFTLTHFLRETPNLRLRIFVSRLYFCDEEDSSAREGLRMLKKAGVNITVMSYKDYFYCWKTFVAHRQRNFKAWDGLDQNSVHLARKLSHILQPWETADLRDAFKLLGL-- Helostoma_temminckii MITKLDSVLLPRKKFIYHYKNVRWARGRHETYLCFVVKRRVGPDSLTFDFGHLRNRNGCHVE-MLFLRYL--GALCPGLWGCGGTGE-RRLSYSITWFCSWSPCSNCSLRLAQFLSQTPNLRLRIFVSRLYFCDMEDSREREGLRILKNAGVQITVMSYKDFFYCWQTFVARKQSNFKAWEELHQNSVRLTRKLHRILQPCETEDLRDAFKLLGL-- Holocentrus_rufus MITKLDSVLLAKKKFIYHYKNLRWAKGRHETYLCFVVKRRAGPDSIAFDFGHLRNRAGCHVE-LLFLRYL--GALCPGLWGYGGTGE-RKMSYSITWFCSWSPCANCSYRLAQFLNRTPNLRLRLFVARLYFCDIEDSREREGLRMLKNAGVHITVMSYKDYFYCWQTFVARKTSNFKAWDGLHQNYVRLARKLNRILQPCETEDLRDAFRLLGL-- Laemonema_laureysi MISKLDSVLLAQKKFMFNYKNMRWARGRNETYLCFVVKRRLGPNSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCRGDEN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEDSPHIEGLRDLRRAGVRVTVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLT Lampris_guttauts MISKLDSVLLTQKKFLYHYKNVRWAKGRHETYLCFVVKRRVGPDSMSFDFGHLRNRAGCHVE-LLFLRYL--GALCPGLWGYGDTGD-RRLSYSVTWFCSWSPCANCSFRLAQFLQRTPNFRLRLFVSRLYFCDMEDSSERDGLRLLKNAGVQITVMSYKDYFYCWQTFVAHRKSSFKAWDGLHQNTVRLARLLNRILQPCEAEDLRDAFKLLGF-- Lamprogrammus_exutus MIAKLDSVLLPRKKFIFHYKNMRWAKGRHETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGCGGSGE-RRLSYSITWFCSWSPCANCSQRLSQFLSQTPNLRLRIFVSRLYFCDMENSREREGLRMLKNAGVQITVMSYKDFFYCWQTFVACGKSKFKAWDELHRNSVRLTRKLNRILQPWETEDLRDAFRLLGF-- Lesueurigobius_cf_sanzoi MITKLDSVLLPKKKFIFHYKNVRWAKGRHETYLCFVVKRRVGPNSMSFDFGHLRNRSGCHVE-ILFLRYL--GALCPGLWGAGGSEE-RRLSYSITWFCSWSPCANCSTKLSQFLAKTPNLRLRIFVSRLYFCDLEDSIEREGLRMLKRAGVQLTVMKYKDYFYCWHTFVARNQSNFKAWEELHQNSVRLTRKLSRILQPCETEDLRDAFRLLGL-- Lota_lota MISKLDSVLLAQKKFIYNYKNIRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCGGDRN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEGSPHIEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLFGLLT Macrourus_berglax MISKLDSILLAQKKFKYNYNNMRWAKGRNETYLCFVVKRRLGPNSLSFDFGHLRNRAGCHVE-LLFLSHL--GALCPGLWGFGGAEN-IRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCELADSPHSEGLRELRRAGVQVNVMTYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNLILQPCETEDLRDAFRLIGLLT Malacocephalus_occidentalis MISKLDSVLLAQKKFIYNYKNIRWAKGRNETYLCFVVKRRLGPNSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCGGADN-RRLNYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLDDSPHTEGLRELRRAGVQFTVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLS Melanogrammus_aeglefinus MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCAGDRN-RRLSYSVTWFCSWSPCANCATTLTRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDVFRLFGLLT Melanonus_zugmayeri MISNLDSVLLAQKKFMYNYKNMHWAKGRNATYLCFVVKRRLGPDSLSFDFGHLHNRTGCHAE-LLFLSHL--GALCPGLWGCGGDKN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARVYFCEQEDSPHIEGLRDLRRAGVQVTVMSYKDYFYCWQTFVAHRLSRFKTWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLT Merlangius_merlangus MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCAGDRN-RRLSYSVTWFCSWSPCANCATTLSRFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDVFRLFGLLT Merluccius_merluccius MISKLDSVLLAQKKFMYNYKNMRWAKGRNQTYLCFVVKRRLGPDSLSFDFGHLHNRTGCHAE-LLFLSHL--GALCPGLWGCGGDEN-RRLSYSVTWFCSWSPCANCAATLARFLRLTPNLRLRIFVARLYFCDVEDSPHREGLRNLRRAGVLVNVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRTLNRILQPCETEDLRDAFRLIGLLT Merluccius_polli MISKLDSVLLAQKKFMYNYKNMRWAKGRNQTYLCFVVKRRLGPDSLSFDFGHLHNRTGCHAE-LLFLSHL--GALCPGLWGCGGDEN-RRLSYSVTWFCSWSPCANCAATLARFLRLTPNLRLRIFVARLYFCDVEDSPHREGLRNLRRAGVLVNVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRTLNRILQPCETEDLRDAFRLIGLLT Molva_molva MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCGGDTN-RRLSYSVTWFCSWSPCANCAATLARFLRHTPNLRLRIFVARLYFCDLEGSPHIEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHKLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDAFRLFGLLT Monocentris_japonica MITKLDSVLLAQKKFIYHYKNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGLWGYGGTGE-RRLSYSITWFCSWSPCADCSFRLVQFLGRTPNLRLRIFVSRLYFCDVEDSRERQGLRMLKKAGVQITVMSYKDYFYCWQTFVAHRQSSFKAWDELHQNSVRLARKLNRILQPCETEDLRDAFKLLGL-- Mora_moro MISTLDSVLLAQKKFMYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHVE-LLFLSHL--GALCPGLWGCGGDEN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEDSPHIEGLRDLRRAGVQVTVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLT Muraenolepis_marmoratus MISKLDSVLLGQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSMSFDFGHLRNRAGCHVE-LLFLSHL--GALCPGLWGCGGDEN-RRLSYSVTWFCSWSPCANCAATLARLLRQTPNLRLRIFVARLYFCDLEGSPHSEGLRDLRRAGVQVNVMSYKDYFYCWQTFVAHRVSRFKAWEGLHTNSVRLSRKLNRILQPRETDDLRDAFRLIGLLT Myoxocephalus_scorpius MITKLDSVLLQQKKFIYHYKNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRTGCHVE-LLFLRYL--GALCPGLWGYGGTGE-KRLSYSITWFCSWSPCINCSISLSQFLNRTPNLRLRIFVSRLYFCDKENSRERDGLRMLKNAGVQITVMSYKDFFYCWQTFVDRKKSNFKAWEELHQNSVRLARKLNRILQPCEAEDLRDAFKLLGL-- Myripristis_jacobus MITKLDSMLLAKKKFIYHYKNMRWAKGRHETYLCFVVKRRVGPDSMSFDFGHLRNRAGCHVE-LLFLRYL--GALCPGLWGCGGNTE-KKLSYSITWFCSWSPCADCSFRLAQFLNRTPNLRLRIFVSRLYFCDLEDSREREGLRMLKKAGVQITVMSYKDYFYCWQTFVAHRMSSFKAWDGLHQNYVRLARKLNRILQASETEDLRDAFKLLGL-- Neoniphon_sammara MITKLDSVLLAKKKFIYHYKNLRWAKGRHETYLCFVVKRRVGPDSIAFDFGHLRNRAGCHVE-LLFLRYL--GALCPGLWGYGGTGE-RKLSYSITWFCSWSPCANCSFRLAQFLNRTPNLRLRIFVSRLYFCDVEDSREREGLRMLKNAGVHITVMSYKDYFYCWQTFVARKTSSFKAWDGLHQNYVRLARKLNRILQPCDTEDLRDAFRLLGL-- Oreochromis_niloticus MIAKLDSMLLPRKKFLYHYKNVRWARGRNETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVE-LLFLRQL--GTLCPGLSGYGFHGE-RRVSYSITWFCSWSPCANCSSRLAQFLKQTPNLRLRIFVSRLYFCDMEDSREREGLRLLKKVGVHITVMSYKDFFYCWENFVA-QQSKFKAWEGLHQNTVRLARKLNRILQPCDTEDLRDAFKLLGL-- Oryzias_latipes MITKLDSVLLPKKKFIYHYKNMRWARGRHETYLCFVVKRRVGPESLSFDFGHLRNRNGCHVE-LLFLRHL--SALCPGLWGYGATGQ-GRVSYSITWFCSWSPCANCSFRLAQFLSQTPNLRLRIFVSRLYFCDLEDSREREGLRMLKKVGVHITVMSYKDYFYCWQTFVARKQSKFKPWDGLHQNSVRLSRKLNRILQPCETEDFRDAFKLLGL-- Osmerus_eperlanus MISTLDGVLLAQKKFIYHYKNMRWARGRHETYLCFVIKRRVGPDSLSFDFGHLRNRTGCHVE-LLFLRHL--GALCPGLWGTGGAGGGVRLSYSITWFCSWSPCSACSHRLSDFLSRTPNLRLRIFVSRLYFCDPEDSLEREGLRMLKRAGVNITVMSYKDYFYCWETFVARRKTGFKAWDGLHHNSVRLARKLYRILQPCETEDLRDAFTLLGL-- Parablennius_parvicornis MIAKLDSMLLPRKKFIYHYKNMRWAKGRHETYLCFVVKRRLGPDSLSFDFGHLRNRNGCHVE-LLFLRHL--GTLCPGLSGYGVHGE-KRLSYSITWFCSWSPCSNCSHRLAQFLSRTPNIRLRIFVSRLYFCDLEDSREREGLRLLKKTGVHITVMSYKDYFYCWQTFVASNQSRFKPWDELQRNSIRLTRKLNRILQPCETEDLRDAFKLLGL-- Parasudis_fraserbrunneri MITNLDSVLLAQKKFIYHYKNMRWARGRHETYLCFVVKRRLGPDSLSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGLWGYGGE---KRLSYSVTWFCSWSPCADCSTRLSQFLSRTPNLRLRIFVSRLYFCDLEDSLAREGLRTLKRVGVQVTVMSYKDYFYCWQTFVARRQSSFKAWDGLQQNSVRLARKLNRILQPCETEDLRDAFKLLGL-- Perca_fluviatilis MITKLDSVLLPRKKFIYHYKNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVE-LLFLRYI--GALCPGLWGCSGTGE-RRLSYSITWFCSWSPCANCSIRLSQFLSQTPNLRLRIFVSRLYFCDTENSPERDGLRMLKKAGVQITVMSYKDFFYCWQTFVDRKQSNFKAWEELHSNSVRLSRKLNRILQPFETEDLRDAFKLLGL-- Percopsis_transmontana MITKLDSVLLAQKKFIFHYKNMRWARGRHETYLCFVIKRRVGPNSLSFDFGHLRNRSGCHVE-ILFLRHL--GALCPGLWGEGGTGE-RRLSYSITWFCSWSPCANCSLRLAQILRQLPNLRLRIFVSRLYFCDLEDSKERDGLRMLKNVGVQITVMSYKDYFYCWQTFVAHRKSNFKAWDGLHQNSVRLARKLNRILQPCEIEDLRDAFKLLGF-- Phycis_blennoides MISKLDSVLLAQKKFIYNYKNIRWAKGRNETYLCFVVKRRLGPNSLSFDFGHLRNRAGCHVE-LLFLSHL--GALCPGLWGCVDDSN-RRLSYSVTWFCSWSPCANCAATLARFLRMTPNLRLRIFVARLYFCDLEDSPHIEGLRHLRRAGVEVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLFGLLT Phycis_phycis MISKLDSVLLAQKKFLYNYKNIRWAKGRNETFLCFVVKRRLGPNSLSFDFGHLRNRAGCHVE-LLFLSHL--GALCPGLWGCVDDSN-RRLSYSVTWFCSWSPCANCAATLARFLRMTPNLRLRIFVARLYFCDLEDSPHIEGLRHLRRAGVEVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLFGLLT Poecilia_formosa MITKLDRALLPRKKFIYHYKNLRWARGRCETYLCFVVKKRVGPDSLSFDFGHLRNRNNCHVE-LLFLRHL--GALCPGLWGYGVTGE-RKVSYSVTWFCSWSPCANCSIRLAQFLHQTPNLRLRIFVSRLYFCDLEDSREREGLRILKKAGVHITVMSYKDYFYCWQTFVAKSQSKFKPWDGLHQNYIRLSRKLNRILQPCETEDLRDAFRLLGL-- Pollachius_virens MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRTGCHAE-LLFLSYL--GALCPGLWGCADDRN-RRLIYSVTWFCSWSPCANCATTLARFLRQTPNLRLRIFVSRLYFCDLEGSPHVEGLRDLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNYVRLSRKLNRILQPCETEDLRDVFGLFGLLT Polymixia_japonica MITKLDSVLLAQKKFIYHYKNMRWAKGRHETYLCFVVKRRVGPDSMSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGLWGYGGTGE-KRLSYSVTWFCSWSPCSNCSYRLAQFLSQTPNLRLRIFVSRLYFCDLEDSRERDGLRMLKRAGVQITVMTYKDYFYCWQTFVAHRTSKFKAWDELHRNSVRLSRILNRILQPCETEDLRDAFRLLGL-- Pseudochromis_fuscus MIAKLDSVLLPKKKFIFHYKNMRWARGRHETYLCFVVKRRRGPDSLSFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGYGATGA-SRLSYSITWFCSWSPCANCSFRLAQFLSQTPNLRLRIFVSRLYFCDMEDSREREGLRQLKKAGVHITVMSYKDYFYCWQTFVARNQSKFKPWDELHQNSVRLSRKLNRILQPCETEDLRDAFKLLGL-- Rondeletia_loricata MITKLDSVLLAKKKFIYHYKNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRTGCHVE-LLFLRHL--GALCPGLWGHGGTGE-RRLSYSITWFCSWSPCADCSFRLAQFLGRMPNLRLRIFVSRLYFCDLEDSREREGLRLLKKAGVQITVMSYKDFFYCWQTFVAHRNCSFKAWDEMHQNSVRLARKLNRILQPCETEDLRDAFKLLGL-- Salmo_salar_1 MINKFDSVLLAQKKFIYHYKNMRWAKGRHETYLCFVVKRRVGPNSLSFDFGHLRNRSGCHVE-LLFLRLLEAGALCPGLWGYGAPDS-VGLCYSVTWFCSWSPCSDCSYRLAQFLSQTPNLRLRIYVSRLYFCDPEDSSAREGLRMLQRAGVQITVMNYEDYFYCWQTFVACRQRVFKAWDGLHQNSVQLARKLNDILQPGEAEDWGDAFELLGL-- Salmo_salar_2 MINKFDSVLLAQKKFIYHYKNMRWAKGRHETYLCFVVKRRGGPNSLSFDFGHLRNRSGCHVE-LLFLRLLEAGALCPGLWGYGAPDS-VGLCYSVTWFCSWSPCSDCSYRLAQFLSQTPNLRLRIYVSRLYFCDPEDSSAREGLRMLQRAGVQITVMNYEDYFYCWQTFVACRQRVFKAWDGLHQNSVQLARKLNDILQPGEAEDWGDAFELLGL-- Sebastes_norvegicus MITKLDSVLLPRKKFIFHYKNMRWARGRHETYLCFVVKRRVGPDSLTFDFGHLRNRNGCHVE-LLFMRYL--GALCPGLWGQGVPGE-KRLSYSITWFCSWSPCVNCSVTLSQFLSKTPNLRLRIFVSRLYFCDMENSRERDGLRMLKKAGVQISVMSYKDYFYCWQTFVDRKQSKFKAWDEMHQNSVRLTRKLSRILQPSETEDLRDAFKLLGL-- Selene_dorsalis MITKLDSVLLPRKKFIFHYKNVRWAKGRHETYLCFVVKRRVGPDSMTFDFGHLRNRNGCHVE-ILFLRYL--GALCPGLWGYGVGGE-KRLSYSITWFCSWSPCANCSSRLAQFLKQTPNLRLRIFVSRLYFCDLEDSQEREGLRILKKAGVHITVMTYKDFFYCWQTFVARKQSSFKAWDELHQNSVRLARKLQRILQPCETEDLRDAFKLLGL-- Spondyliosoma_cantharus MITKLDSVLLPKKKFIYHYKNVRWARGRHETYLCFVVKRRVGPDTLTFDFGHLRNRNGIHVE-LLFLRYL--GALCPGLWGYGGTGE-KRLSYSITWFCSWSPCANCSLRLCQFLSQTPNLRLRIFVSRLYFCDMEDSREREGLRMLKKAGVQITVMSYKDFFYCWQTFVARRASQFKAWEELQRNSVRLTRKLNRILQPCETEDLRDAFKLLGL-- Stylephorus_chordatus MIAKLDSVLLARNKFIYHYKNMRWAKGRNETYLCFVVKRRVGPDSLAFDFGHLRNRTGCHVE-LLFLRHL--GALCPGLWG-GAAGD-KRLSYSVTWFCSWSPCANCASTLAQFLRQTPNLRLRLFVARLYFCDLEDSPDREGLRILRRAGVHITVMRYKDYFYCWQTFVAHNQSRFKAWEGLHPNSVRLSRTLNRILQPCETEDLRDAFKLLGL-- Symphodus_melops MNTKLDSVLLPRKKFIYHYKNVRWARGRHETYLCFVIKRRVGPDTLTFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLLGYGGAGE-KRLSYSITWFCSWSPCSNCSTILCQFLSKMPNLRLRLFVSRLYFCDMEDSREREGLRMLKKVGVQITIMSYKDFFYCWQKFVARRQSNFKAWEELHQNSVRLSRKLNRILQPCETEDLRDAFKLLGL-- Takifugu_rubripes MITKLDSMLLPRKKFIYHYKNVRWARGRHETYLCFVVKRRVGPDTLTFDFGHLRNRSGCHVE-LLFLRYL--GALCPGLWGYGAAGE-KRLSYSVTWFCSWSPCVNCSIQLCQFLNNTPNLRLRIFVSRLYFCDLEDSLEREGLRMLTKAGVRISVMSYKDYFYCWQKFVDCKKSNFKAWEELHQNSVRLTRKLNRILQAWDLEDLRDALKLLGF-- Tetraodon_nigroviridis MITKLDSMLLPRKKFLYHYKNVRWARGRHETYLCFVVKRRVGPDTLTFDFGHLRNRNGCHVE-LLFLRYL--GALCPGLWGYGAAGE-KRLSYSITWFCSWSPCANCSIQLSQFLRNTPNLRLRIFVSRLYFCDMEDSLEREGLRMLSRAGVRISVMSYKDFFYCWQKFVDSKTSSFKAWEELHQNSVRLTRKLNRILQSWDLEDLRDALKLLGL-- Thunnus_albacares MITKLDSVLLPRKKFIYHYKNVRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVE-LLFLRYL--GALCPGVWGYGNTGQ--RISYSITWFCSWSPCANCSRRLAQFLSQVPNVRLRIFVSRLYFCDLEDSRERDGLRLLKNAGVQITVMSYKDFFYCWQTFVARNQSKFKAWEELHRNSVRLTRTLNRILQPCDIDDLRDAFKLLGL-- Trachyrincus_murrayi MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRLGCHVE-LLFLSHL--GALCPGLWGCGGDVN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEDSPHIEGLRDLRRAGVQVTVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLT Trachyrincus_scabrus MISKLDSVLLAQKKFIYNYKNMRWAKGRNETYLCFVVKRRLGPDSLSFDFGHLRNRLGCHVE-LLFLSHL--GALCPGLWGCGGDEN-RRLSYSVTWFCSWSPCANCAATLARFLRHTPNLRLRIFVARLYFCDLEDSPHIEGLRDLRRAGVQVTVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDAFRLIGLLT Trisopterus_minutus MISKLDSVLLAQKKFIYNYKNLRWAKGRNETYLCYVVKRRLGPDSLSFDFGHLRNRTGCHVE-LLFLSYL--GALCPGLWGCTDDRN-RRLSYSVTWFCSWSPCANCAATLARFLRQTPNLRLRIFVARLYFCDLEGSPHIEGLRHLRRAGVQVKVMSYKDYFYCWQTFVAHRLSRFKAWEGLHTNSVRLSRKLNRILQPCETEDLRDVFGLFGLLT Typhlichthys_subterraneus MISKLDSVLLAQKKFIFHYKNMRWARGRNETYLCFVIKRRVGPDSLSFDFGHLRNRSGCHVE-LLFLRHL--GALCPGLWGQGGTGD-NRLSYSITWFCSWSPCSNCSHRLAQFLSQLPNLRLRIFVSRLYFCDLEDSREREGLRMLKNAGVHITVMSYKDYYYCWQTFVARRKSKFKAWEGLHQNSVRLARKLNRILQPCEIEDLRDAFKLLGF-- Xiphophorus_maculatus MITKLDRVLLPKKKFIYHYKNMRWARGRCETYLCFVVKKRVGPDSLSFDFGHLRNRNNCHVE-LLFLRHL--GALCPGLWGYGVTGE-RKVSYSITWFCSWSPCANCSFRLAQFLHQTPNLRLRIFVSRLYFCDLEDSREREGLRMLKKAGVHITVMSYKDYFYCWQTFVAKSQSKFKPWDGLHQNCIRLTRKLNRILQPCETEDLRDAFRLLGL-- Zeus_faber MITKLDSVLLARKKFIYHYKNMRWAKGRCETYLCFVVKRRVGPNSLSFDFGHLRNRTGCHVE-LLFLRHL--GALCPGLWGHGGPYG-GRLSYSVTWFCSWSPCANCSFRLAQFLGQTPNLRLRIFVSRLYYCDLEDSREREGLRILKRAGVQITVMSYKDYFYCWQTFVAHRQTSFKAWDELHQNSVRLARKLNRILQPCETEDLRDAFKLLGFL- ; End; BEGIN TREES; TREE part_1 = ((Astyanax_mexicanus:121.77122741279602,Danio_rerio:121.77122741279602):101.99953637657163,((((((((((((((((Gasterosteus_aculeatus:55.40747769565583,Myoxocephalus_scorpius:55.40747769565583):22.627589007329938,(Sebastes_norvegicus:71.41027871780396,Chaenocephalus_aceratus:71.41027871780396):6.624787975823878):5.948298200333113,Perca_fluviatilis:83.98336487731933):27.234997279977804,((((Takifugu_rubripes:46.743550480651855,Tetraodon_nigroviridis:46.743550480651855):52.31449333152771,Antennarius_striatus:99.05804377441406):4.952094098567969,Spondyliosoma_cantharus:104.0101378944397):3.8940865391969623,Symphodus_melops:107.90422446670532):3.314137740588194):4.0983751179695105,(((Oreochromis_niloticus:90.12568147794833,(Oryzias_latipes:69.76327566986083,(Poecilia_formosa:18.384480726242064,Xiphophorus_maculatus:18.384480726242064):51.37879498329163):20.362405779966025):6.229750216332775,((Chromis_chromis:86.03637034606933,Pseudochromis_fuscus:86.03637034606933):6.271233430540818,Parablennius_parvicornis:92.30760380917813):4.047827894961586):15.657636823177327,((Helostoma_temminckii:64.19328314971924,Anabas_testudineus:64.19328314971924):42.49635722122191,Selene_dorsalis:106.6896403640747):5.32342815576196):3.303668787118795):2.6957501523196754,(Thunnus_albacares:103.80823429222107,Lesueurigobius_cf_sanzoi:103.80823429222107):14.204253155434131):5.149373299789431,Chatrabus_melanurus:123.16186078948975):4.574033951210964,((Lamprogrammus_exutus:66.95888729228973,Carapus_acus:66.95888729228973):23.925274275398266,Brotula_barbata:90.88416159934998):36.85173311395644):9.028980841016761,((Myripristis_jacobus:59.01862996520996,(Holocentrus_rufus:14.652058449554444,Neoniphon_sammara:14.652058449554444):44.366571516036984):70.12873428974152,((Rondeletia_loricata:89.71703486652375,Acanthochaenus_luetkenii:89.71703486652375):23.455124383091928,Beryx_splendens:113.17215923690796):15.97520504798888):7.61751127405762):3.0117428180396644,Monocentris_japonica:139.776618334198):7.268487315320982,Lampris_guttauts:147.04510569152832):3.839686785376074,(((((((Molva_molva:42.47743926963806,(Brosme_brosme:39.03891726341247,(((((Arctogadus_glacialis:5.222854929506778,Boreogadus_saida:5.222854929506778):2.4513389710009097,(Theragra_chalcogramma:3.346329225230217,Gadus_morhua:3.346329225230217):4.327864675396681):5.726030785477162,(Melanogrammus_aeglefinus:10.395505192184448,Merlangius_merlangus:10.395505192184448):3.0047194936364896):4.48665917098522,Pollachius_virens:17.886883866405487):9.021208262825013,(Trisopterus_minutus:22.696635680580137,Gadiculus_argenteus:22.696635680580137):4.211456434738636):12.130825152540204):3.4385220028579297):3.19094077802896,Lota_lota:45.6683800485611):13.098774013638497,(Phycis_blennoides:16.400560005474087,Phycis_phycis:16.400560005474087):42.366594044685364):12.221774325680741,((Merluccius_merluccius:5.7998921918630600,Merluccius_polli:5.7998921924829485):61.73268190526217,(Melanonus_zugmayeri:63.95622381646633,((((Macrourus_berglax:29.67249945344925,Malacocephalus_occidentalis:29.67249945344925):20.17204357004166,Bathygadus_melanobranchus:49.84454303512574):10.645187737723738,(Mora_moro:36.98188234682083,Laemonema_laureysi:36.98188234682083):23.5078484090443):1.939285897175786,(Muraenolepis_marmoratus:56.88453414344788,(Trachyrincus_scabrus:12.07161490740776,Trachyrincus_murrayi:12.07161490740776):44.81291924285889):5.5444825193190255):1.5272070992565432):3.576350340722499):3.4563542779281846):33.47187371263503,Stylephorus_chordatus:104.46080207824707):21.041643343019487,(Zeus_faber:32.85098531341553,Cyttopsis_roseus:32.85098531341553):92.65146017112733):19.170966317129142,(Polymixia_japonica:135.7494994041443,(Percopsis_transmontana:60.18133554153442,Typhlichthys_subterraneus:60.18133554153442):75.56816387424469):8.923912356662754):6.211380692934995):7.560750375115873,Benthosema_glaciale:158.44554285736086):10.299416859668469,(Parasudis_fraserbrunneri:161.78278560620342,Guentherus_altivela:161.78278560077345):6.962174173392896):25.783923850154878,(Osmerus_eperlanus:117.3724450843811,Borostomias_antarcticus:117.3724450843811):77.15643841142654):17.481817657327667,(Salmo_salar_1:0.00662978935994194395,Salmo_salar_2:0.00857699553333491141):212.0107011795044):11.760062609915053); END; BEGIN ASSUMPTIONS; CHARSET span_1 = 1-217; END;