GFP expression and phenotypic notes following knockdown of genes tested with piRNA reporter. All worms were screened as adults or 3 days post-L1 if adulthood was difficult to determine. ,,,,,,,,
"RNAi strategy for each gene is noted. The default RNAi strategy of L4 treatment and screening in subsequent generation is indicated with ""L4"". If this treatment procedure resulted in early developmentally arrested progeny, then an alternative strategy was used. Treatment from L1 to adulthood is indicated with ""L1"". Treatment from L2 to adult is indicated with ""L2"".",,,,,,,,
Gene name,Locus name,Wormbase ID,Description,% GFP expression,total worms screened,technical replicates,RNAi strategy,Phenotype
air-1,K07C11.2,WBGene00000098,"Cell cycle, P granule regulator",0,5,1,L4,Low broods
arx-5,Y37D8A.1,WBGene00000203,Nuclear pore component,0,33,1,L4,Some dead embryos
bir-1,T27F2.3,WBGene00000249,"Cell cycle, P granule regulator",0,12,1,L4,Possibly dumpy
cdc-25.1,K06A5.7,WBGene00000386,"Cell cycle, P granule regulator",43.9,41,2,L1,"Odd germ cell appearance, cell fate transformation likely"
cdl-1,R06F6.1,WBGene00000411,RNAi resistant,89.3,28,1,L4,Poorly developed germline
cpf-2,F56A8.6,WBGene00000774,RNAi resistant,0,23,1,L4,Burst vulvas
crn-3,C14A4.4,WBGene00000796,Exosome component,0,7,1,L1,Slow development
dcs-1,Y113G7A.9,WBGene00000940,Decapping enzyme homolog,0,32,1,L4,No obvious phenotype
dic-1/ints-6,F08B4.1,WBGene00000994,Integrator complex,67.7,48,4,L4,Poorly developed germline
egl-45,C27D11.1,WBGene00001209,"Translation initiation, P granule regulator",0,11,1,L2,"Very sick, poorly developled germline"
eif-3.F,D2013.7,WBGene00001229,"Translation initiation, P granule regulator",0,24,1,L2,Poorly developed germline
fib-1,T01C3.7,WBGene00001423,nucleolar protein,89.4,41,4,L4,Poorly developed germline
snpc-4,F32H2.1,WBGene00001568,piRNA biogenesis factor,92,>150,>10,L1,Poorly developed germline
hpl-2,K01G5.2,WBGene00001996,HP1-like protein,25.1,31,3,L4,Slightly delayed development
ima-1,T19B10.7,WBGene00002072,Nuclear transport,7.9,28,2,L4,Some dead embryos
ima-2,F26B1.3,WBGene00002073,Nuclear transport,12.5,28,2,L4,"Poorly developed germline, dead embryos"
ima-3,F32E10.4,WBGene00002074,Nuclear transport,100,30,2,L4,"Poorly developed germline, dead embryos"
imb-2,R06A4.4,WBGene00002076,Nuclear transport,36.1,29,2,L4,"Poorly developed germline, dead embryos"
imb-4/xpo-1,ZK742.1,WBGene00002078,Nuclear transport,0,19,1,L4,Poorly developed germline
imb-5/xpo-2,Y48G1A.5,WBGene00002079,Nuclear transport,67.6,36,2,L4,"Poorly developed germline, dead embryos"
mcm-2,Y17G7B.5,WBGene00003154,P granule regulator,0,8,1,L4,"Sick, low broods"
unc-32,ZK637.8,WBGene00003154,P granule regulator,0,9,1,L4,Motility defects
mel-28,C38D4.3,WBGene00003210,Nuclear pore component,0,9,1,L4,Some dead embryos
mes-3,F54C1.3,WBGene00003221,H3K27 histone methyl transferase,72.7,22,1,L4,"Poorly developed germline, dead embryos"
mes-4,Y2H9A.1,WBGene00003222,H3K36 histone methyl transferase,54.6,17,1,L4,Slightly reduced brood
mog-1,K03H1.2,WBGene00003389,pre-mRNA splicing,0,28,3,L1,Very pooly developed germline
mog-4,C04H5.6,WBGene00003392,pre-mRNA splicing,0,18,1,L4,Dead embryos
mog-5,EEED8.5,WBGene00003393,pre-mRNA splicing,0,21,3,L1,Very pooly developed germline
dcap-2,F52G2.1,WBGene00003582,"Decapping enzyme, present in P body and P granule",0,37,1,L4,Poorly developed germline
npp-1,K07F5.13,WBGene00003787,Nuclear pore component,57.9,23,3,L4,"Dead embryos, poorly developed germline"
npp-2,T01G9.4,WBGene00003788,Nuclear pore component,3.7,27,3,L4,"No embryos, poorly developed germline"
npp-3,K12D12.2,WBGene00003789,Nuclear pore component,3.3,25,3,L4,"Dead embryos, poorly developed germline"
npp-4,Y54E5A.4,WBGene00003790,Nuclear pore component,0,26,3,L4,"Dead embryos, poorly developed germline"
npp-5,F07A11.3,WBGene00003791,Nuclear pore component,0,33,3,L4,Some dead embryos
npp-6,F56A3.3,WBGene00003792,"Nuclear pore component, P granule regulator",67.5,40,4,L4,Poorly developed germline
npp-7,T19B4.2,WBGene00003793,"Nuclear pore component, P granule regulator",77.4,85,6,L4,Poorly developed germline
npp-9,F59A2.1,WBGene00003795,"Nuclear pore component, P granule regulator",58.1,43,4,L4,Some dead embryos
npp-10,ZK328.5,WBGene00003796,"Nuclear pore component, P granule regulator",9.1,28,3,L4,Some dead embryos
npp-14,C03D6.4,WBGene00003800,Nuclear pore component,8.9,29,3,L4,Some dead embryos
npp-15,C29E4.4,WBGene00003801,Nuclear pore component,0,30,3,L4,Some dead embryos
npp-16,Y56A3A.17,WBGene00003802,Nuclear pore component,6.7,28,3,L4,Poorly developed germline
npp-17/rae-1,F10G8.3,WBGene00003803,Nuclear pore component,0,31,1,L4,Some dead embryos
npp-18,Y43F4B.4,WBGene00003804,Nuclear pore component,6.7,26,3,L4,Some dead embryos
npp-19,R06F6.5,WBGene00003805,Nuclear pore component,0,28,3,L4,Some dead embryos
npp-20,Y77E11A.13,WBGene00003806,"Nuclear pore component, P granule regulator",10,21,3,L4,Some dead embryos
pas-4,C36B1.4,WBGene00003925,"Proteasome, P granule regulator",0,10,1,L2,"Very poor development, no obvious germ cells"
pas-5,F25H2.9,WBGene00003926,"Proteasome, P granule regulator",100,8,1,L2,"Sterile, possible cell fate transformation"
pbs-3,Y38A8.2,WBGene00003949,"Proteasome, P granule regulator",0,16,1,L2,Poorly developed germline
prp-21,W07E6.4,WBGene00004188,"pre-mRNA splicing, RNAi defective",56.7,30,3,L4,Dead embryos
rsp-7,D2089.1,WBGene00004704,"pre-mRNA splicing, RNAi defective",0,27,1,L4,Poorly formed embryos
smu-2,Y49F6B.4,WBGene00004896,pre-mRNA splicing,0,12,1,L4,No obvious phenotype
uaf-2,Y116A8C.35,WBGene00006698,pre-mRNA splicing,0,14,1,L4,Poorly developed germline
npp-22,B0240.4,WBGene00007118,Nuclear pore component,0,29,1,L4,Poorly developed germline
npp-23,C09G9.2,WBGene00007493,Nuclear pore component,0,30,3,L4,~25% worms with poorly developed germline
nrde-1,C14B1.6,WBGene00007577,hrde-1 cofactor,34.2,32,3,L4,No obvious phenotype
prp-4,C36B1.5,WBGene00007972,pre-mRNA splicing,5.6,33,3,L4,Dead embryos
ints-7,D1043.1,WBGene00008361,Integrator complex,0,47,3,L4,Poorly developed germline
E02H1.1,E02H1.1,WBGene00008455,RNAi resistant,0,26,1,L4,Mostly no embryos
ints-11,F10B5.8,WBGene00008642,Integrator complex,71.4,38,4,L1,"Poorly developed germline, dead embryos"
ncbp-2,F26A3.2,WBGene00009141,"pre-mRNA splicing, RNAi defective",0,27,1,L4,No embryos
snrp-27,R05D11.7,WBGene00011035,pre-mRNA splicing,0,15,1,L4,Slightly delayed development
paa-1,R186.7,WBGene00011308,"PP2A subunit A, RNAi resistant",0,30,1,L2,"Very poor development, no obvious germ cells"
nrde-2,T01E8.5,WBGene00011333,hrde-1 cofactor,44.3,22,3,L4,No obvious phenotype
T25G3.3,T25G3.3,WBGene00012030,"rRNA processing, RNAi resistant",0,26,1,L1,"No embryos, some burst vulvas"
tofu-5,W01A8.5,WBGene00012167,piRNA biogenesis factor,56.9,29,3,L4,Some dead embryos
ints-4,W04A4.5,WBGene00012234,Integrator complex,0,45,3,L4,"Very poor development, poorly developed germline"
set-25,Y43F4B.3,WBGene00012802,H3K9 histone methyl transferase,35.9,28,3,L4,No obvious phenotype
ints-5,Y51A2D.7,WBGene00013075,Integrator complex,0,30,3,L4,"Very poor development, poorly developed germline"
prp-40,ZK1098.1,WBGene00014218,pre-mRNA splicing,6.7,37,3,L4,Dead embryos
ints-1,C06A5.1,WBGene00015499,Integrator complex,68.8,50,4,L4,Poorly developed germline
ddx-23,F01F1.7,WBGene00017162,pre-mRNA splicing,6.1,32,3,L4,Some dead embryos
cpsf-2,F09G2.4,WBGene00017313,"mRNA processing, RNAi defective",0,23,1,L4,Burst vulvas
ints-9,F19F10.12,WBGene00017608,Integrator complex,63.6,32,4,L1,"Poorly developed germline, dead embryos"
tofu-2,F20A1.9,WBGene00017620,piRNA biogenesis factor,20.4,31,3,L4,Some dead embryos
csr-1,F20D12.1,WBGene00017641,P granule enriched Argonaute protein,45.7,36,3,L4,"Very poor development, poorly developed germline"
prp-17,F49D11.1,WBGene00018625,"pre-mRNA splicing, RNAi defective",77.4,27,3,L4,Poorly developed germline
pup-2,K10D2.2,WBGene00019628,polyU polymerase,0,7,1,L1,Poorly developed germline
sago-1,K12B6.1,WBGene00019666,RNAi resistant,0,26,1,L4,Some dead embryos
K12H4.5,K12H4.5,WBGene00019680,RNAi resistant,0,31,1,L4,Dead embryos
ints-13,R02D3.4,WBGene00019822,Integrator complex,0,40,3,L4,No obvious phenotype
nrde-3,R04A9.2,WBGene00019862,somatic nuclear Argonaute,2.8,34,3,L4,No obvious phenotype
npp-21,R07G3.3,WBGene00019940,Nuclear pore component,0,31,1,L4,"Dead embryos, poorly developed germline"
T19B4.5,T19B4.5,WBGene00020558,RNAi resistant,0,28,1,L4,Dead embryos
ints-12/phf-30,T23B12.1,WBGene00020716,Integrator complex,0,45,3,L4,"No obvious phenotype, slightly lower broods"
npp-24,ZK177.4,WBGene00022672,Nuclear pore component,3.3,32,3,L4,No obvious phenotype
ZK1127.5,ZK1127.5,WBGene00022852,"rRNA processing, RNAi resistant",0,28,1,L2,No obvious germ cells