sgRNA,Gene,D1.Pre,D2.Pre,Spleen.M6,Spleen.M7,Spleen.M8,Spleen.M9,Tumor.M6,Tumor.M7,Tumor.M8,Tumor.M9 NonTarget.CTRL2_TGCTACCTTCGGGACCACCA,NonTarget.CTRL2,8642,12171,1220,3190,1782,1907,5212,5733,2440,1722 PDCD1_CACCTACCTAAGAACCATCC,PDCD1,23124,26659,3166,6952,3846,4360,15194,14438,6794,4401 ARID1A_TTTGAATGCAAGATTGAACG,ARID1A,2065,1728,287,582,558,589,1937,1654,984,633 TMEM222_GACTCACTGAGACAAAGTAG,TMEM222,15958,21730,3545,7660,4600,3768,17999,13813,7205,3859 TNFRSF9_ACATTTAACGATCAGAAACG,TNFRSF9,10489,14894,1494,3709,2996,2671,8189,6808,4204,2976 MEF2D_CATCATCCCTCACGGACCCG,MEF2D,17219,15301,2719,5040,3210,3415,8469,7984,4513,3459 STAT6_CAGCCACCACAAAGGGCACG,STAT6,12143,15007,2040,3465,2744,2951,8381,8093,5190,2191 DGKZ_GAATAAGATGTTCTACGCCG,DGKZ,4254,3823,620,1265,819,779,3474,2079,1424,1346 CD3D_GACAATGATGCCAGCCACGG,CD3D,12650,9972,2442,4804,3191,3047,8803,5835,3103,2120 CD3D_ACTTCGATAATGAACTTGCA,CD3D,13759,14996,2962,6540,2845,3233,8655,5663,3555,2179 RASA2_AGATATCACACATTACAGTG,RASA2,14505,21763,2885,5819,2751,3516,10482,8342,5545,4873 STAT6_GCTGGAAGGCCTCCATACTG,STAT6,11018,11218,1787,4219,2210,2397,8846,8839,4143,2698 CBLB_TGCACAGAACTATCGTACCA,CBLB,23370,27715,3486,8069,4107,4333,16421,14649,6918,4967 NonTarget.CTRL2_CGAACTTCTGGCTGCAGTTT,NonTarget.CTRL2,15543,12771,2190,4578,3156,3389,9955,9026,5193,3127 NonTarget.CTRL1_CGTCGCCATATGCCGGTGGC,NonTarget.CTRL1,12763,11677,1701,4959,2248,2406,7032,7098,2835,1956 CD5_AAGCGTCAAAAGTCTGCCAG,CD5,15332,15741,2743,6535,2644,2890,12777,10564,5479,3941 TCEB2_CGAACTGAAGCGCATCGTCG,TCEB2,13062,17003,2255,4109,2519,3202,12346,8131,4220,3838 BTLA_TGTTAGCACAGTATTTCACA,BTLA,4626,3636,672,1766,811,961,2485,2660,2272,717 DGKZ_CTCTCCTCAGTACCACAGCA,DGKZ,30853,23956,5487,11363,7280,6788,23110,20047,11918,7414 PDCD1_AGGGCCCGGCGCAATGACAG,PDCD1,26553,27694,5172,11831,6586,6842,22822,18618,12178,6831 CBLB_TTCCGCAAAATAGAGCCCCA,CBLB,24494,26048,4181,8347,5562,5834,18188,15175,8553,5738 NonTarget.CTRL1_TAAGCCTCATGAAGGAGGGG,NonTarget.CTRL1,8479,7211,1456,3051,1949,1759,7844,7172,4126,2685 SOCS1_CGGCGTGCGAACGGAATGTG,SOCS1,23496,28368,4042,7521,4697,4703,21129,18308,10586,6153 C10orf54_CTCTGCCCTTTCAGACATCA,C10orf54,23796,22556,4493,8648,5604,5407,19353,16429,9193,5352 RASA2_TTAGCATCAAGGCATGCCAT,RASA2,3455,6185,699,1311,522,821,2978,2953,1643,515 NonTarget.CTRL1_ATTGCGCAATCCTTAGGATA,NonTarget.CTRL1,13276,19854,3026,6089,3686,3556,13299,10188,7517,4300 SOCS1_GGGCCCCCAGTAGAATCCGC,SOCS1,7213,15224,1777,2878,2291,1927,6776,6133,2521,1661 CD5_CGGCTCAGCTGGTATGACCC,CD5,16720,15093,1809,4710,2586,2627,8375,9062,4700,2756 HAVCR2_GTGAAGTCTCTCTGCCGAGT,HAVCR2,14109,16311,2752,5703,3826,3435,12958,10152,6531,4158 MEF2D_CAAGTACCGACGCGCCAGCG,MEF2D,33834,44324,4011,10319,6971,7238,21773,21185,9843,5231 CDKN1B_GGGCAAGTACGAGTGGCAAG,CDKN1B,9099,6928,1463,4248,2404,2155,7346,5865,4888,3927 TMEM222_ACGGACATGAAGCAATATCA,TMEM222,17838,19733,3371,7164,3981,3917,17425,13874,7726,4206 HAVCR2_TGTGTTTGAATGTGGCAACG,HAVCR2,25564,24122,4222,9558,6057,6227,21332,17436,10309,5467 LCP2_GGAGCTAATGAACGATCTAG,LCP2,9574,7654,1382,3712,2022,2243,6869,5426,3602,1918 NonTarget.CTRL1_CTCGGAATACTCCTATTACA,NonTarget.CTRL1,8619,10201,1717,3531,1838,1922,7845,6438,3228,1982 NonTarget.CTRL2_CTAAGTTTGTTAATGGGCCA,NonTarget.CTRL2,7214,7168,1343,3161,1716,1770,7054,5438,2466,1768 ARID1A_CAGCAGAACTCTCACGACCA,ARID1A,22001,17537,3267,6450,4433,4555,20183,15871,9888,7680 NonTarget.CTRL2_GCCCCGTAAATCTCATTACA,NonTarget.CTRL2,28884,29958,5190,11542,6151,6607,24975,20253,10589,7201 BTLA_TCACATAAAGAGTTGTTGAG,BTLA,36157,33945,7242,15087,8703,8951,31455,26482,17145,10222 TCEB2_GAGCAGCGGCTGTACAAGGT,TCEB2,5706,17174,1366,2573,1437,1756,5524,5454,2359,1253 TNFRSF9_TGTGCTTGTGAATGGGACGA,TNFRSF9,18911,15029,2301,5436,3670,3625,13407,10902,6491,3929 LAG3_CTCAGCACCGTGTAGCGGCG,LAG3,13157,12151,2167,5300,2593,2866,9895,7941,6019,2925 CDKN1B_TGGACCACGAAGAGTTAACC,CDKN1B,20725,26165,3572,7390,4577,4813,17681,13909,5668,4287 DGKA_CTCCCAAACTGACCTCTAGG,DGKA,22155,22509,4225,8382,4707,4512,17981,14273,7910,6051 LAG3_GTTCCGGAACCAATGCACAG,LAG3,13651,22501,2511,5908,3021,3437,12375,11110,7513,3620 DGKA_GATTGACAAAGACGAGAAGT,DGKA,43627,41922,6756,13981,9399,8916,29641,25946,15051,8246 C10orf54_GACATACAGGGAATACGGCG,C10orf54,11164,9886,1781,3574,2174,1960,8685,6404,4576,2116 LCP2_AGCCACGAAGAGGACAATGG,LCP2,24856,19115,5373,10736,7473,5677,16773,13873,7714,5902