Dataset S5. Phylogenetic tree of Tas2r genes in vertebrates. Vertebrate Tas2r sequences were obtained from the supplementary data of Li and Zhang (2014), except for those of the 20 frogs examined in this study.

(Fish_V1r:1.67488241455,((((zebrafish_Tas2r4:0.1145529273,zebrafish_Tas2r2:0.2501593146)100:1.1235153140,(Takifugu_Tas2r2:0.9497446433,Cod_Tas2r5:1.4499417808)85:0.3941406627)100:1.6503335770,((Stickleback_Tas2r3:0.3467047030,(Pufferfish_Tas2r1:0.3319592827,Takifugu_Tas2r1:0.1783872446)99:0.2494312157)100:2.3827849330,(zebrafish_Tas2r1:2.1055659877,(zebrafish_Tas2r3:1.5769995620,(((Pufferfish_Tas2r6:0.5911148721,Takifugu_Tas2r4:0.7055989306)100:1.1497883450,(Cod_Tas2r2:0.3056656580,(Cod_Tas2r4:0.2073042301,(Cod_Tas2r3:0.2634394590,Cod_Tas2r1:0.2340476262)64:0.0637182985)60:0.0676167925)100:0.8629640997)87:0.2883798004,((Stickleback_Tas2r2:0.0778212528,Stickleback_Tas2r1:0.1359565699)100:1.1322520070,(Takifugu_Tas2r3:0.6029983543,(Pufferfish_Tas2r3:0.4261069720,(Pufferfish_Tas2r4:0.1594893330,(Pufferfish_Tas2r5:0.0331632469,Pufferfish_Tas2r2:0.0488248368)100:0.2393973390)94:0.1448746796)100:0.3081506323)100:0.6695525265)100:0.6875792313)69:0.2261143187)73:0.2492027361)100:1.5860203640)75:0.2265809883)47:0.1116394529,((Coelacanth_Tas2r5:0.3953177474,(Coelacanth_Tas2r1:0.9207359525,(Coelacanth_Tas2r6:0.3982754245,(Coelacanth_Tas2r4:0.4897549872,Coelacanth_Tas2r3:1.2571820017)87:0.0943433239)98:0.1960254491)49:0.1337929829)100:1.3446523620,((((Yunnan_mustache_toad_Leptobrachium_ailaonicum_1635:0.0038359976,Leishan_spiny_toad_Leptobrachium_leishanense_871:0.0220809664)100:0.8085006909,(Yellow-bellied_toad_Bombina_variegata_1601:0.7008486653,Asiatic_toad_Bufo_gargarizans_206:0.8560382063)53:0.1324529888)92:0.2191813055,((Asiatic_toad_Bufo_gargarizans_234:0.9464383671,(Puerto_Rican_coqui_Eleutherodactylus_coqui_1797:0.7898580108,Asiatic_toad_Bufo_gargarizans_223:0.4539002266)89:0.2238840251)100:0.3944629482,(African_bullfrog_Pyxicephalus_adspersus_30:0.5533786079,(Tibetan_Plateau_frog_Nanorana_parkeri_1361:0.3423563726,((Common_frog_Rana_temporaria_408:0.4728740252,American_bullfrog_TAS2R101:0.1059854686)100:0.3741443039,(Common_frog_Rana_temporaria_377:0.1895530308,American_bullfrog_TAS2R100:0.0837763632)100:0.204155450)80:0.0741976566)80:0.1029541977)100:0.4442691193)100:0.4111179060)100:1.4617433540,(((anole_lizard_Tas2r121:0.7881460199,anole_lizard_Tas2r35:0.7525475337)75:0.1575281444,(anole_lizard_Tas2r43:0.5378870721,(anole_lizard_Tas2r40:1.0361685687,(anole_lizard_Tas2r13:0.3213290329,(anole_lizard_Tas2r16:0.3413680989,(anole_lizard_Tas2r20:0.3731807129,anole_lizard_Tas2r191:0.3480446377)98:0.11378445)35:0.0609159564)100:0.5286610507)21:0.0847968234)28:0.1085221180)100:1.6323366530,(((((((Common_toad_Bufo_bufo_508:0.0244586636,Asiatic_toad_Bufo_gargarizans_146:0.0114490513)89:0.0267205860,(Common_toad_Bufo_bufo_461:0.0344649070,Asiatic_toad_Bufo_gargarizans_138:0.0114816235)100:0.0813296655)100:0.3306938107,(Tibetan_Plateau_frog_Nanorana_parkeri_1343:0.0927606983,(African_bullfrog_Pyxicephalus_adspersus_70:0.1453296173,(Japanese_wrinkled_frog_Glandirana_rugosa_758:0.0316815269,(Common_frog_Rana_temporaria_321:0.0101665737,(American_bullfrog_TAS2R85:0.0154196440,American_bullfrog_TAS2R43:0.0104067119)99:0.0210164220)94:0.0102046303)100:0.2056039795)70:0.0437278437)100:0.1924074745)100:0.5047254989,(Yellow-bellied_toad_Bombina_variegata_1607:0.6605822193,((((Mexican_spadefoot_toad_Spea_multiplicata_1041:0.0000027079)100:0.4001904725,(Yunnan_mustache_toad_Leptobrachium_ailaonicum_1689:0.0081802573,Leishan_spiny_toad_Leptobrachium_leishanense_925:0.0133344048)100:0.3801681778)55:0.0581605043,(((Tungara_frog_Engystomops_pustulosus_1547:0.2455318801,(Strawberry_poison_frog_Oophaga_pumilio_1260:0.1279760525)100:0.2025622443)100:0.2779157297,(Ornate_burrowing_frog_Platyplectrum_ornatum_1142:0.2185291040,Eastern_banjo_frog_Limnodynastes_dumerilii_641:0.1503160084)100:0.2040510644)56:0.0248466833,(Tibetan_Plateau_frog_Nanorana_parkeri_1355:0.0870250572,(Common_frog_Rana_temporaria_418:0.0656819163,(Japanese_wrinkled_frog_Glandirana_rugosa_787:0.0787576638,American_bullfrog_TAS2R97:0.0426355514)96:0.0213390840)98:0.0958213614)100:0.2418041295)100:0.2189144964)41:0.0556474319,(Yellow-bellied_toad_Bombina_variegata_1574:0.4999731577,((Yellow-bellied_toad_Bombina_variegata_1569:0.3552437405,(Yunnan_mustache_toad_Leptobrachium_ailaonicum_1674:0.0086510074,Leishan_spiny_toad_Leptobrachium_leishanense_885:0.0027319413)100:0.2550722188)29:0.0646596170,(Mexican_spadefoot_toad_Spea_multiplicata_1007:0.3890452742,((Eastern_banjo_frog_Limnodynastes_dumerilii_591:0.2656136030,((Tungara_frog_Engystomops_pustulosus_1529:0.0000010081)100:0.1544310889,(Common_toad_Bufo_bufo_477:0.0426090222,Asiatic_toad_Bufo_gargarizans_209:0.0045967830)100:0.1080118906)100:0.0953178272)62:0.0464860046,(Tibetan_Plateau_frog_Nanorana_parkeri_1373:0.0873034044,(Japanese_wrinkled_frog_Glandirana_rugosa_683:0.0313470783,(Common_frog_Rana_temporaria_335:0.0409617547,American_bullfrog_TAS2R84:0.0249799047)97:0.0220907421)100:0.0585819651)99:0.0936827135)95:0.103970030)82:0.081987069)23:0.035360843)73:0.1007969367)52:0.0767542986)100:0.2310872014)100:0.5276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