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. Author manuscript; available in PMC: 2015 Jul 24.
Published in final edited form as: Cell Prolif. 2009 Jul 9;42(5):672–687. doi: 10.1111/j.1365-2184.2009.00629.x

Table 1.

Necrosis scoring of male and female mouse lung sections at different time points after treatment with three different doses of naphthalene

Time (days) after naphthalene injection Mean necrosis score/time point after naphthalene injection
Low dose (50 mg/kg)
Medium dose (100 mg/kg)
High dose (200 mg/kg)
Male Female Male Female Male Female
0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0
2 0.33 ± 0.03 1.03 ± 0.03a,b 1.20 ± 0.0b 2.03 ± 0.09a,b 2.30 ± 0.06b 2.93 ± 0.05a,b
14 0.07 ± 0.03 0.17 ± 0.03 0.07 ± 0.03 0.17 ± 0.03 0.17 ± 0.03 1.00 ± 0.05a,b
21 0.0 ± 0.0 0.03 ± 0.03 0.0 ± 0.0 0.07 ± 0.03 0.05 ± 0.03 0.20 ± 0.04

Histopathological analysis of male and female mouse lung sections at 0, 2, 14, and 21 days after injection with low (50 mg/kg), medium (100 mg/kg), or high dose (200 mg/kg) of naphthalene, as shown in Fig. 1, was performed and the extent of bronchiolar epithelial (Clara) cell necrosis was scored. See the Materials and methods for details. Data are presented as the mean necrosis score ± standard error of three to seven mice per gender at each time point after treatment with each dose of naphthalene.

a

Significantly different from that of male mice at same time point after treatment with same dose of naphthalene (P < 0.05).

b

Significantly different from the 0 days control group (P < 0.05). The degree of bronchiolar epithelial (Clara) cell necrosis was estimated semi-quantitatively, and was expressed for each mouse as the mean of 10 random fields within each section (one section per mouse) classified on a scale of 0–3. Scoring criteria were as follows: 0, no necrosis, defined as no detection of necrotic bronchiolar epithelial cells exfoliated within the airway lumen; 1, mild, defined as only occasional detection of necrotic bronchiolar epithelial cells exfoliated within the airway lumen; 2, moderate, defined as more frequent detection of necrotic bronchiolar epithelial cells exfoliated within the airway lumen; 3, severe, defined as very frequent detection of numerous necrotic bronchiolar epithelial cells within the airway lumen.