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. Author manuscript; available in PMC: 2010 May 20.
Published in final edited form as: J Neurosci. 2005 Aug 31;25(35):8037–8047. doi: 10.1523/JNEUROSCI.1903-05.2005

Figure 4.

Figure 4

Src tyrosine kinase inhibits the cation channel. A, Top trace, An excised control channel in the presence of 1 mM ATP (PO = 0.020). Bottom trace, Application of 10 U/ml recombinant Src tyrosine kinase to the cytoplasmic face of the patch decreases the activity of the channel (PO = 0.002). Representative of 11 experiments. The patch was held at −60 mV. B, Top trace, Another excised cation channel again in the presence of ATP (PO = 0.0254). Bottom trace, Application of a mixture of 10 U/ml recombinant Src tyrosine kinase and 5 μM of the Src-family inhibitor PP1 does not result in any significant change in activity (PO = 0.0286). PP1 and Src were mixed for ~1 min before their addition. Representative of seven experiments. The patch was held at −60 mV. Amplitude and time base calibration bars also apply to A.