Figure 3.

IFN-λ4 confers a mixed phenotype on THP-1-derived macrophage-like cells. (A) Histogram from one representative experiment showing that IFN-λ4-treated macrophage-like cells had increased expression of CD80 but reduced expression of HLA-DR (left) when activated with LPS, whereas IFN-λ4 had no effect when activation was carried out with LPS and IFN-λ. The graph on the right shows the normalized (for mock IFN-λ4 treatment; error bars show _SD. The significance was calculated using a one-sample t-test (*P < 0.05). (B and C, right) mRNA expression of diftreatment) mean of positive events in the gate shown in the histogram and/or mean fluorescence intensity from two independent experiments with ferent genes from M1-activated (with LPS) (B) and M2-activated (with macrophage CSF [M-CSF], IL-4, and IL-10) (C) THP-1-derived macrophagereplicate experiments. *P < 0.05; **P < 0.01, ***P < 0.001. (C, left) Histogram of CD209-, CD206-, and CD163-positive cells as determined by flow like cells. The data show the mean from technical triplicates with error bars depicting _SD, and are representative of at least two separate biological cytometry of THP1-derived M2 macrophage-like cells differentiated in the absence or presence of IFN-λ4 (6 μg/ml). The data were obtained from a single experiment