Figure 9.

Comparison of fold changes in cytokine secretion in cocultures of CD4⁺ cells and monocyte-derived dendritic cells (MoDCs) differentiated in the presence of IFN-λ3 or IFN-λ4. MoDCs were differentiated from CD14⁺ cells obtained from four donors in the absence or presence of IFN-λ3 or IFN-λ4; coculture experiments were set up with CD4⁺ cells from 20 allogenic donors and cytokines were collected from supernatants ated in the presence of IFN-λ3 (left) or IFN-λ4 (right) and allogenic CD4⁺ cells. The color key at the bottom shows row Z-score. The comparisons and measured by ELISA. (A) Heatmap showing the fold changes of five cytokines collected from coculture supernatants involving MoDCs generComparison of cytokine profiles between 20 allogenic CD4⁺ donors in coculture experiments involving MoDCs derived in presence of IFN-λ3 or shown below the heatmap are between any two cytokines by paired t-tests. Only the comparisons that were statistically significant are shown. (B) IFN-λ4. The comparison is between IFN-λ3 and IFN-λ4 for a given cytokine. The bars depict mean values and error bars depict _SD. A 2-tailed t-test significant differences in their means for IFN-λ3; for IFN-λ4, however, a 1-tailed t-test showed significance in some new comparisons: IFN-γ vs. IL-4 for dependent means was used for all statistical comparisons in (A) and (B); a 1-tailed t-test did not show any new groups of cytokines that had (P = 0.03) and IL-4 vs. IL-17 (P = 0.03). For (A) and (B) *P < 0.05, **P < 0.01; ns, not significant