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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Leukemia. 2020 Jun 24;34(11):2951–2963. doi: 10.1038/s41375-020-0929-3

Figure 4. Loss-of-function shRNA-screening identifies functional dependencies in CMML#1-MN1 cells.

Figure 4

A) Experimental outline of loss-of-function shRNA screening showing lentiviral transduction of CMML#1-MN1 cells with a shRNA pool, transplantation into NSGS recipient mice, cell harvest after eight weeks and quantification of shRNAs by next-generation sequencing.

B) Representation of shRNAs in blood, bone marrow and spleen of NSGS recipient mice at eight weeks after transplantation compared to baseline shRNA representation (baseline: transduced cells kept in vitro and quantified on day 3), (each column represents one mouse, n=6).