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. Author manuscript; available in PMC: 2021 Mar 16.
Published in final edited form as: Cell Stem Cell. 2020 Dec 2;28(3):453–471.e8. doi: 10.1016/j.stem.2020.11.005

Figure 2. Lineage potency of FS cells and responsiveness to differentiation cues.

Figure 2

(A) Neural differentiation assayed by quantification of Sox1::GFP-positive cells. Error bars represent S.D. from 4 independent experiments. (B) Immunostaining of FS cells and EpiSCs during neural differentiation, DAPI in white. Scale bars, 100 μm. (C) Lateral plate mesoderm differentiation and representative quantifications of the Flk1 +Ecad- fractions by flow cytometry. (D) Average efficiency of Flk1 positive cell production from FS cells and EpiSCs. n = independent cell lines assayed. Error bars represent the S.D. **P<0.01. (E) Definitive endoderm differentiation protocol and representative quantifications of the Cxcr4+Ecad+ fraction. (F) Average proportion of Cxcr4+Ecad+ double positive cells from differentiation of FS and EpiSC lines. Error bars represent S.D., *P<0.05. (G) T expression analysed by RT-qPCR 6h and 24h after transfer into N2B27 medium with the indicated supplements; 2μM XAV939, 20ng/ml activin A, 10ng/ml BMP2, 12.5ng/ml Fgf2 and 3μM CH. Relative expression is normalised to GAPDH. Error bars are S.D. from two independent cell lines and two technical replicates.